Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0661
(487 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g01690.1 68415.m00096 expressed protein 89 1e-18
At2g01690.2 68415.m00097 expressed protein 85 2e-17
At5g28830.1 68418.m03546 calcium-binding EF hand family protein ... 31 0.54
At1g79610.1 68414.m09282 sodium proton exchanger, putative (NHX6... 30 0.95
At5g24100.1 68418.m02830 leucine-rich repeat transmembrane prote... 29 1.3
At2g15440.1 68415.m01766 expressed protein contains Pfam profile... 29 1.3
At4g38200.1 68417.m05392 guanine nucleotide exchange family prot... 29 2.2
At4g16563.1 68417.m02506 aspartyl protease family protein contai... 29 2.2
At1g54370.1 68414.m06199 sodium proton exchanger, putative (NHX5... 29 2.2
At5g13760.1 68418.m01604 expressed protein similar to unknown pr... 28 2.9
At3g15980.3 68416.m02022 coatomer protein complex, subunit beta ... 28 3.8
At3g15980.2 68416.m02021 coatomer protein complex, subunit beta ... 28 3.8
At3g15980.1 68416.m02020 coatomer protein complex, subunit beta ... 28 3.8
At3g58460.1 68416.m06516 rhomboid family protein / ubiquitin-ass... 27 8.9
At3g42950.1 68416.m04511 glycoside hydrolase family 28 protein /... 27 8.9
At1g52360.1 68414.m05909 coatomer protein complex, subunit beta ... 27 8.9
At1g21750.2 68414.m02723 protein disulfide isomerase, putative s... 27 8.9
At1g21750.1 68414.m02722 protein disulfide isomerase, putative s... 27 8.9
At1g13420.1 68414.m01566 sulfotransferase family protein similar... 27 8.9
>At2g01690.1 68415.m00096 expressed protein
Length = 743
Score = 89.4 bits (212), Expect = 1e-18
Identities = 45/99 (45%), Positives = 62/99 (62%)
Frame = +1
Query: 190 YEKRSTYGSVYCSSGAWQGISSYLPELTNPIVACFSESESRVRYQAAEALFNVLKIVRGA 369
+ K G + G + YL ++ P++ FS+ +SRVRY A EAL+N+ K+VRG
Sbjct: 66 HRKGGLIGLAAVTVGLSTEAAQYLEQIVPPVINSFSDQDSRVRYYACEALYNIAKVVRGD 125
Query: 370 SLSQFPVIFDALAKLAADPEQQVKHAAELLDRLLKDIVT 486
+ F IFDAL KL+AD + V+ AA LLDRL+KDIVT
Sbjct: 126 FIIFFNKIFDALCKLSADSDANVQSAAHLLDRLVKDIVT 164
Score = 60.5 bits (140), Expect = 6e-10
Identities = 29/68 (42%), Positives = 44/68 (64%)
Frame = +2
Query: 35 RGLCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALMG 214
R L DKLYEKRK A +E+E +VK+ + + +I K+I++L ++ S N + G L+G
Sbjct: 14 RNLSDKLYEKRKNAALELENIVKNLTSSGDHDKISKVIEMLIKEFAKSPQANHRKGGLIG 73
Query: 215 LSTVAVGL 238
L+ V VGL
Sbjct: 74 LAAVTVGL 81
>At2g01690.2 68415.m00097 expressed protein
Length = 744
Score = 85.0 bits (201), Expect = 2e-17
Identities = 45/100 (45%), Positives = 62/100 (62%), Gaps = 1/100 (1%)
Frame = +1
Query: 190 YEKRSTYGSVYCSSGAWQGISSYLPELTNPIVACFSESESRVRYQAAEALFNVLKIVRGA 369
+ K G + G + YL ++ P++ FS+ +SRVRY A EAL+N+ K+VRG
Sbjct: 66 HRKGGLIGLAAVTVGLSTEAAQYLEQIVPPVINSFSDQDSRVRYYACEALYNIAKVVRGD 125
Query: 370 SLSQFPVIFDALAKLAADPEQQVKHAAELLDRLLK-DIVT 486
+ F IFDAL KL+AD + V+ AA LLDRL+K DIVT
Sbjct: 126 FIIFFNKIFDALCKLSADSDANVQSAAHLLDRLVKQDIVT 165
Score = 60.5 bits (140), Expect = 6e-10
Identities = 29/68 (42%), Positives = 44/68 (64%)
Frame = +2
Query: 35 RGLCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALMG 214
R L DKLYEKRK A +E+E +VK+ + + +I K+I++L ++ S N + G L+G
Sbjct: 14 RNLSDKLYEKRKNAALELENIVKNLTSSGDHDKISKVIEMLIKEFAKSPQANHRKGGLIG 73
Query: 215 LSTVAVGL 238
L+ V VGL
Sbjct: 74 LAAVTVGL 81
>At5g28830.1 68418.m03546 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 324
Score = 30.7 bits (66), Expect = 0.54
Identities = 15/38 (39%), Positives = 27/38 (71%), Gaps = 2/38 (5%)
Frame = +1
Query: 373 LSQFPVIFDALAKLAADPEQQVKHA--AELLDRLLKDI 480
LS+FP++ D + K AD ++++ A AELL ++L++I
Sbjct: 161 LSEFPILDDIIKKHDADSDEELGQAQFAELLQQVLQEI 198
>At1g79610.1 68414.m09282 sodium proton exchanger, putative (NHX6)
identical to Na+/H+ exchanger 6 [Arabidopsis thaliana]
gi|19919848|gb|AAM08407 (Plant J. (2002) In press);
contains similarity to Na+/H+ antiporter GI:1655701 from
[Xenopus laevis]; contains non-consensus AT/AC splice
sites; Member of The Monovalent Cation:Proton Antiporter
(CPA1) Family, PMID:11500563
Length = 535
Score = 29.9 bits (64), Expect = 0.95
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = -2
Query: 360 DNLQHVEQCLSCLVPYTRFALAE 292
DNLQ++E CL L PY + LAE
Sbjct: 253 DNLQNLECCLFVLFPYFSYMLAE 275
>At5g24100.1 68418.m02830 leucine-rich repeat transmembrane protein
kinase, putative
Length = 614
Score = 29.5 bits (63), Expect = 1.3
Identities = 17/41 (41%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
Frame = +1
Query: 214 SVYCSSGAWQGISSY-LPELTNPIVACFSESESRVRYQAAE 333
+V+ +S + IS LP LTNP+V S + S +RY+A E
Sbjct: 470 NVFMNSEGYGCISEAGLPLLTNPVVRADSSARSVLRYRAPE 510
>At2g15440.1 68415.m01766 expressed protein contains Pfam profile
PF04669: Protein of unknown function (DUF579)
Length = 329
Score = 29.5 bits (63), Expect = 1.3
Identities = 16/40 (40%), Positives = 25/40 (62%)
Frame = -2
Query: 141 FFIWLVFFASLKSFTIFSISTPATFLFSYNLSQSPRTQAM 22
F +L FF L SF++FS S +T S++LS SP + ++
Sbjct: 36 FIFFLSFFTLLFSFSLFSSSIHST-TSSHSLSSSPSSSSL 74
>At4g38200.1 68417.m05392 guanine nucleotide exchange family protein
similar to Brefeldin A-inhibited guanine
nucleotide-exchange protein 2 [Homo sapiens] SP|Q9Y6D5;
contains Pfam profile PF01369: Sec7 domain
Length = 1698
Score = 28.7 bits (61), Expect = 2.2
Identities = 15/38 (39%), Positives = 23/38 (60%)
Frame = +1
Query: 241 QGISSYLPELTNPIVACFSESESRVRYQAAEALFNVLK 354
+ IS ++P LT + S+S S +R + E LFN+LK
Sbjct: 1266 ENISYWVPLLTG-LSKLTSDSRSAIRKSSLEVLFNILK 1302
>At4g16563.1 68417.m02506 aspartyl protease family protein contains
Pfam profile: PF00026 eukaryotic aspartyl protease
Length = 499
Score = 28.7 bits (61), Expect = 2.2
Identities = 14/46 (30%), Positives = 24/46 (52%)
Frame = -2
Query: 156 KTLINFFIWLVFFASLKSFTIFSISTPATFLFSYNLSQSPRTQAML 19
KT + FF++ F++ S+STP S++LS S + + L
Sbjct: 2 KTCLIFFLYTTILQYYFHFSVSSLSTPLLLHLSHSLSTSKHSSSPL 47
>At1g54370.1 68414.m06199 sodium proton exchanger, putative (NHX5)
contains similarity to Na+/H+ antiporter GI:1655701 from
[Xenopus laevis]; Member of The Monovalent Cation:Proton
Antiporter (CPA1) Family, PMID:11500563; contains
non-consensus AT-AC splice sites at introns 3 and 10
Length = 521
Score = 28.7 bits (61), Expect = 2.2
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -2
Query: 363 SDNLQHVEQCLSCLVPYTRFALAE 292
++NLQ++E CL L PY + LAE
Sbjct: 252 TENLQNLECCLFVLFPYFSYMLAE 275
>At5g13760.1 68418.m01604 expressed protein similar to unknown
protein (gb AAF63775.1)
Length = 569
Score = 28.3 bits (60), Expect = 2.9
Identities = 12/32 (37%), Positives = 16/32 (50%), Gaps = 1/32 (3%)
Frame = -2
Query: 162 WPKTLINFFIWLVFFASLKS-FTIFSISTPAT 70
WP +I+F +W F SL S + PAT
Sbjct: 213 WPDFMIHFILWSTFLMSLSSGILLLCFQMPAT 244
>At3g15980.3 68416.m02022 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats
(PF00400) (1 weak); identical to coatomer protein
complex, beta prime (beta'-COP) protein {Arabidopsis
thaliana} (GI:9294445); similar to Coatomer beta'
subunit (Beta'-coat protein) (Beta'-COP) (p102)
(SP:P35606) [Homo sapiens]
Length = 918
Score = 27.9 bits (59), Expect = 3.8
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = +1
Query: 253 SYLPELTNPIVACFSESESRVRYQAAEAL 339
SYLP + IVA + E S+V +AAE+L
Sbjct: 761 SYLPSKVSEIVALWREDLSKVNPKAAESL 789
>At3g15980.2 68416.m02021 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats
(PF00400) (1 weak); identical to coatomer protein
complex, beta prime (beta'-COP) protein {Arabidopsis
thaliana} (GI:9294445); similar to Coatomer beta'
subunit (Beta'-coat protein) (Beta'-COP) (p102)
(SP:P35606) [Homo sapiens]
Length = 918
Score = 27.9 bits (59), Expect = 3.8
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = +1
Query: 253 SYLPELTNPIVACFSESESRVRYQAAEAL 339
SYLP + IVA + E S+V +AAE+L
Sbjct: 761 SYLPSKVSEIVALWREDLSKVNPKAAESL 789
>At3g15980.1 68416.m02020 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats
(PF00400) (1 weak); identical to coatomer protein
complex, beta prime (beta'-COP) protein {Arabidopsis
thaliana} (GI:9294445); similar to Coatomer beta'
subunit (Beta'-coat protein) (Beta'-COP) (p102)
(SP:P35606) [Homo sapiens]
Length = 909
Score = 27.9 bits (59), Expect = 3.8
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = +1
Query: 253 SYLPELTNPIVACFSESESRVRYQAAEAL 339
SYLP + IVA + E S+V +AAE+L
Sbjct: 761 SYLPSKVSEIVALWREDLSKVNPKAAESL 789
>At3g58460.1 68416.m06516 rhomboid family protein /
ubiquitin-associated (UBA)/TS-N domain-containing
protein contains Pfam profiles PF01694: Rhomboid family,
PF00627: UBA/TS-N domain
Length = 403
Score = 26.6 bits (56), Expect = 8.9
Identities = 9/26 (34%), Positives = 17/26 (65%)
Frame = +1
Query: 190 YEKRSTYGSVYCSSGAWQGISSYLPE 267
Y ++T S + + AW+ +SS+LP+
Sbjct: 255 YSAQNTTSSGFSTGNAWRSLSSWLPQ 280
>At3g42950.1 68416.m04511 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase precursor [Cucumis melo]
GI:3320460; contains PF00295: Glycosyl hydrolases family
28
Length = 484
Score = 26.6 bits (56), Expect = 8.9
Identities = 20/57 (35%), Positives = 26/57 (45%)
Frame = -2
Query: 183 LVEDIKSWPKTLINFFIWLVFFASLKSFTIFSISTPATFLFSYNLSQSPRTQAMLSG 13
+V S K+LI F W+ FASL + +ST FS S S T + SG
Sbjct: 1 MVRRFLSSHKSLITVF-WIATFASLFIWQFGGVSTNLYSGFSLFWSSSSTTTTVFSG 56
>At1g52360.1 68414.m05909 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats
(PF00400) (1 weak); similar to (SP:O55029) Coatomer
beta' subunit (Beta'-coat protein) (Beta'-COP) (p102)
(SP:O55029) [Mus musculus]; similar to GI:298096 from
[Homo sapiens]
Length = 926
Score = 26.6 bits (56), Expect = 8.9
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = +1
Query: 253 SYLPELTNPIVACFSESESRVRYQAAEAL 339
SYLP + IVA + + S+V +AAE+L
Sbjct: 761 SYLPSKVSEIVALWRKDLSKVNSKAAESL 789
>At1g21750.2 68414.m02723 protein disulfide isomerase, putative
similar to SP|P29828 Protein disulfide isomerase
precursor (PDI) (EC 5.3.4.1) {Medicago sativa}; isoform
contains non-consensus GA donor splice site at intron 9
Length = 487
Score = 26.6 bits (56), Expect = 8.9
Identities = 13/25 (52%), Positives = 14/25 (56%)
Frame = +2
Query: 47 DKLYEKRKVAGVEIEKMVKDFNDAK 121
DK Y K V +IE VKDF D K
Sbjct: 334 DKKYLKTNVEVDQIESWVKDFKDGK 358
>At1g21750.1 68414.m02722 protein disulfide isomerase, putative
similar to SP|P29828 Protein disulfide isomerase
precursor (PDI) (EC 5.3.4.1) {Medicago sativa}; isoform
contains non-consensus GA donor splice site at intron 9
Length = 501
Score = 26.6 bits (56), Expect = 8.9
Identities = 13/25 (52%), Positives = 14/25 (56%)
Frame = +2
Query: 47 DKLYEKRKVAGVEIEKMVKDFNDAK 121
DK Y K V +IE VKDF D K
Sbjct: 334 DKKYLKTNVEVDQIESWVKDFKDGK 358
>At1g13420.1 68414.m01566 sulfotransferase family protein similar to
steroid sulfotransferase 1 GI:3420004 from (Brassica
napus); contains Pfam profile PF00685: Sulfotransferase
domain
Length = 331
Score = 26.6 bits (56), Expect = 8.9
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = +1
Query: 343 NVLKIVRGASLSQFPVIFDALAKLAADPEQQVKHAAELLD 462
NVL RG+ V+F +L +P Q+K AE LD
Sbjct: 211 NVLGYWRGSLEDPKHVLFLRYEELKTEPRVQIKRLAEFLD 250
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,553,811
Number of Sequences: 28952
Number of extensions: 178006
Number of successful extensions: 539
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 527
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 539
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 838967680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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