Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0656
         (735 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr...    83   5e-17
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|...    54   2e-08
SPBC1734.03 ||SPBC337.19|dihydropteroatesynthase/2-amino-4-hydro...    26   4.8  
SPBC211.08c |||ribonuclease PH-like|Schizosaccharomyces pombe|ch...    25   8.5  
SPBC18E5.07 |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    25   8.5  
SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc...    25   8.5  
SPBC19G7.15 |nup44||nucleoporin Nup44|Schizosaccharomyces pombe|...    25   8.5  

>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 591

 Score = 82.6 bits (195), Expect = 5e-17
 Identities = 40/80 (50%), Positives = 52/80 (65%), Gaps = 5/80 (6%)
 Frame = +2

Query: 509 GHPGKTKK-----GELSIIPKNIKLLAPCLHMLPHLHFGLKDKETRFRKRYLDLILNDKV 673
           G+PG++       GELSI  +   LL+PCL MLP  H+GLKD E R R+RYLDLI+N   
Sbjct: 185 GYPGRSNPKGRADGELSIFARQCVLLSPCLRMLPKEHYGLKDLEIRHRQRYLDLIMNRST 244

Query: 674 RQIFYTRAKIIAYVRRFLDN 733
           R  F  R++II Y+R F D+
Sbjct: 245 RDRFVMRSRIIQYIRHFFDS 264



 Score = 72.5 bits (170), Expect = 6e-14
 Identities = 38/79 (48%), Positives = 51/79 (64%)
 Frame = +3

Query: 267 LEEFIEKYQNLNNGDVLENVTLSVAGRVHSIRESGAKLIFYDLRAEGAKIQVMANAKLYE 446
           L EFI KY+ L  G+    V ++VAGRV  +R +G KL FY++ A+G K+QVM  A+  +
Sbjct: 105 LPEFIAKYEGLARGETKPEVEVAVAGRVLGLRTAGNKLRFYEIHADGKKLQVMCQAQDAD 164

Query: 447 TEDKFFKDTDKLRRGDIIG 503
           T D F    + LRRGDIIG
Sbjct: 165 TVD-FAAQHEHLRRGDIIG 182



 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 20/46 (43%), Positives = 33/46 (71%)
 Frame = +1

Query: 121 VAAEKKPSKQEEEISPNEYYKLRSGAVAALKNGLKEDHPYPHKFKL 258
           VAA K  SK+EE++ P++Y++ RS  +  L+   K+ +PYPHKF++
Sbjct: 57  VAAPKSSSKKEEDLDPSQYFENRSRTIMELRQ-TKDPNPYPHKFQV 101



 Score = 25.4 bits (53), Expect = 8.5
 Identities = 7/28 (25%), Positives = 17/28 (60%)
 Frame = +1

Query: 547 YPEKYKIACTMPSYVAAFTFRSQRQRNP 630
           YP K+++  T+P ++A +   ++ +  P
Sbjct: 95  YPHKFQVTITLPEFIAKYEGLARGETKP 122


>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 531

 Score = 54.4 bits (125), Expect = 2e-08
 Identities = 29/73 (39%), Positives = 45/73 (61%), Gaps = 1/73 (1%)
 Frame = +2

Query: 509 GHPGKTKKGELSIIPKNI-KLLAPCLHMLPHLHFGLKDKETRFRKRYLDLILNDKVRQIF 685
           G+ G++  GELSI    + KLL+PCLH +P     L + E RF KR++D++ N K  ++ 
Sbjct: 148 GNVGRSGSGELSIYATELPKLLSPCLHPIP---VKLTNYEKRFEKRFVDMMSNTKSLELL 204

Query: 686 YTRAKIIAYVRRF 724
             R +II  +R+F
Sbjct: 205 EKRYRIIESIRKF 217



 Score = 52.4 bits (120), Expect = 6e-08
 Identities = 31/80 (38%), Positives = 44/80 (55%), Gaps = 4/80 (5%)
 Frame = +3

Query: 279 IEKYQNLNNGDVLENVTLSVAGRVHSIRESGAKLIFYDLRAEGAKIQVMANAKLYETED- 455
           IEK++N      +  V  +V GR+ SIR SG+KL F+D+     K+QV+ N K   TE+ 
Sbjct: 67  IEKWRNKITKSEIAMVRYTVCGRISSIRYSGSKLAFFDVLYGNKKLQVVFNKKNIGTEEE 126

Query: 456 ---KFFKDTDKLRRGDIIGC 506
              KF      L++GD I C
Sbjct: 127 MKGKFIPRLKALQKGDCIQC 146


>SPBC1734.03 ||SPBC337.19|dihydropteroatesynthase/2-amino-4-hydroxy-
           6-
           hydroxymethyldihydropteridinediphosphokinase/dihydroneop
           terinaldolase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 686

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 19/75 (25%), Positives = 36/75 (48%)
 Frame = +3

Query: 267 LEEFIEKYQNLNNGDVLENVTLSVAGRVHSIRESGAKLIFYDLRAEGAKIQVMANAKLYE 446
           L+E + KY++  N     +   ++  +   I+ + + L     + EG  I+V+  + LYE
Sbjct: 236 LQESLHKYESTKNKIAYLSFGSNIGDKFEQIQTALSMLH----KIEG--IRVLDVSPLYE 289

Query: 447 TEDKFFKDTDKLRRG 491
           TE  ++KD      G
Sbjct: 290 TEPMYYKDQPSFLNG 304


>SPBC211.08c |||ribonuclease PH-like|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 257

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 2/32 (6%)
 Frame = +1

Query: 538 TFYYPEKYKIACTM--PSYVAAFTFRSQRQRN 627
           +++  EK KIACT+  P     F FRS  + N
Sbjct: 52  SYFESEKIKIACTVSGPRPSKTFAFRSSAKLN 83


>SPBC18E5.07 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 615

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 15/41 (36%), Positives = 25/41 (60%), Gaps = 6/41 (14%)
 Frame = +1

Query: 124 AAEKKPSKQEEE------ISPNEYYKLRSGAVAALKNGLKE 228
           A+  KPS+  EE      + PN++   R+G VAAL++ L++
Sbjct: 456 ASSTKPSEAAEESTPRFSVRPNKFTGSRAGFVAALESRLQK 496


>SPBC4C3.12 |sep1||fork head transcription factor
           Sep1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 663

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 15/58 (25%), Positives = 29/58 (50%)
 Frame = +3

Query: 297 LNNGDVLENVTLSVAGRVHSIRESGAKLIFYDLRAEGAKIQVMANAKLYETEDKFFKD 470
           L NGD  + VTLS + +  S+ + G+   F +  +  A ++  +   + ++ D F  D
Sbjct: 536 LRNGDASKQVTLSESPKGDSLLDGGSLSYFTNNISSVAGLETPSKLPMSKSFDTFEDD 593


>SPBC19G7.15 |nup44||nucleoporin Nup44|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 403

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 15/46 (32%), Positives = 23/46 (50%)
 Frame = +2

Query: 548 IPKNIKLLAPCLHMLPHLHFGLKDKETRFRKRYLDLILNDKVRQIF 685
           + + I  LA  +H+L H  + LK  E   RK+  DL  +    +IF
Sbjct: 276 LSERILRLAIKVHVLRHRGYALKPNEEELRKKLDDLTKSLNNPEIF 321


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,722,302
Number of Sequences: 5004
Number of extensions: 51851
Number of successful extensions: 129
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)