Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0656
         (735 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin...    89   2e-18
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f...    62   3e-10
At3g52170.1 68416.m05727 expressed protein                             28   5.6  
At5g03970.1 68418.m00377 F-box family protein predicted proteins...    27   9.8  
At3g24600.1 68416.m03090 hypothetical protein                          27   9.8  

>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
           lysine--tRNA ligase, putative similar to SP|Q43776
           Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
           {Lycopersicon esculentum}; contains Pfam profile
           PF00152: tRNA synthetases class II (D, K and N)
          Length = 626

 Score = 89.4 bits (212), Expect = 2e-18
 Identities = 50/96 (52%), Positives = 64/96 (66%), Gaps = 21/96 (21%)
 Frame = +2

Query: 509 GHPGKTKKGELSIIPKNIKLLAPCLHMLPHLH---------------------FGLKDKE 625
           G PGKTK+GELSI P++  LL+ CLHM+P                        + LKD+E
Sbjct: 201 GFPGKTKRGELSIFPRSFILLSHCLHMMPRKADNVNAKKPEIWVPGQTRNPEAYVLKDQE 260

Query: 626 TRFRKRYLDLILNDKVRQIFYTRAKIIAYVRRFLDN 733
           +R+R+R+LD+ILN +VRQIF TRAKII+YVRRFLDN
Sbjct: 261 SRYRQRHLDMILNVEVRQIFRTRAKIISYVRRFLDN 296



 Score = 75.4 bits (177), Expect = 4e-14
 Identities = 39/90 (43%), Positives = 56/90 (62%), Gaps = 1/90 (1%)
 Frame = +3

Query: 267 LEEFIEKYQNLNNGDVLENVTLSVAGRVHSIRESGAKLIFYDLRAEGAKIQVMANA-KLY 443
           + ++IE Y +LNNGD +EN   S+AGR+ S R S +KL FYDL  +  K+QVMA+A K  
Sbjct: 119 IPKYIETYGSLNNGDHVENAEESLAGRIMSKRSSSSKLFFYDLHGDDFKVQVMADASKSG 178

Query: 444 ETEDKFFKDTDKLRRGDIIGCVAIQVKLRK 533
             E +F K     +RGDI+G +    K ++
Sbjct: 179 LDEAEFLKLHSNAKRGDIVGVIGFPGKTKR 208



 Score = 34.7 bits (76), Expect = 0.064
 Identities = 17/51 (33%), Positives = 30/51 (58%)
 Frame = +1

Query: 109 QQXVVAAEKKPSKQEEEISPNEYYKLRSGAVAALKNGLKEDHPYPHKFKLA 261
           Q    +++K  +  +EE+   +YY+ R   +AA K   K ++PYPHKF ++
Sbjct: 68  QAPKASSQKAVAADDEEMDATQYYENRLKYLAAEK--AKGENPYPHKFAVS 116


>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
           family protein similar to SP|Q9RHV9 Lysyl-tRNA
           synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
           stearothermophilus}; contains Pfam profile: PF00152 tRNA
           synthetases class II (D, K and N)
          Length = 602

 Score = 62.5 bits (145), Expect = 3e-10
 Identities = 29/75 (38%), Positives = 48/75 (64%)
 Frame = +2

Query: 509 GHPGKTKKGELSIIPKNIKLLAPCLHMLPHLHFGLKDKETRFRKRYLDLILNDKVRQIFY 688
           G   +T+KGELSI   +  +L   L  LP  + GL D + R+R+RY+D+I N +V  +F 
Sbjct: 194 GSMKRTEKGELSICVNSFSILTKSLLPLPDKYHGLTDIDKRYRQRYVDMIANPEVADVFR 253

Query: 689 TRAKIIAYVRRFLDN 733
            RAKI++ +R+ +++
Sbjct: 254 RRAKIVSEIRKTVES 268


>At3g52170.1 68416.m05727 expressed protein
          Length = 499

 Score = 28.3 bits (60), Expect = 5.6
 Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
 Frame = +3

Query: 267 LEEFIEKYQNLNNGDV--LENVTLSVAGRVHSIRESGAKLI 383
           +E FI+K+Q LNNG    L      V G  ++IRE   ++I
Sbjct: 39  VESFIKKHQKLNNGSFPSLSLTHKEVGGSFYTIREIVREII 79


>At5g03970.1 68418.m00377 F-box family protein predicted proteins,
           Arabidopsis thaliana
          Length = 418

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 11/36 (30%), Positives = 20/36 (55%)
 Frame = +3

Query: 369 GAKLIFYDLRAEGAKIQVMANAKLYETEDKFFKDTD 476
           G K+  YD  +E  +I V+   K Y+ +++   +TD
Sbjct: 242 GGKIAIYDSNSEEKRIDVIKLPKTYDYDEQVLGETD 277


>At3g24600.1 68416.m03090 hypothetical protein
          Length = 506

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 13/41 (31%), Positives = 21/41 (51%)
 Frame = +3

Query: 507 VAIQVKLRKENFLLSRKI*NCLHHAFICCRIYISVSKTKKP 629
           V ++ ++R    LL + +    H   + C  +IS SKT KP
Sbjct: 454 VKLEFEIRSRGNLLGKLV-KSKHENHVSCSFFISSSKTSKP 493


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,836,370
Number of Sequences: 28952
Number of extensions: 257809
Number of successful extensions: 571
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 549
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 569
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)