Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0652
(647 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 128 3e-30
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 83 2e-16
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 80 1e-15
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 78 4e-15
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 78 4e-15
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 60 2e-09
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 46 2e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 46 2e-05
At5g41090.1 68418.m04995 no apical meristem (NAM) family protein... 31 0.50
At2g47500.1 68415.m05929 kinesin motor protein-related 30 1.2
At5g26210.1 68418.m03119 PHD finger family protein contains Pfam... 30 1.5
At3g42790.1 68416.m04474 PHD finger family protein contains PHD-... 28 6.1
At1g55540.1 68414.m06356 proline-rich family protein contains pr... 28 6.1
At5g38500.1 68418.m04654 hypothetical protein contains Pfam prof... 27 8.1
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 27 8.1
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 27 8.1
At2g46240.1 68415.m05750 IQ domain-containing protein / BAG doma... 27 8.1
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 128 bits (309), Expect = 3e-30
Identities = 65/92 (70%), Positives = 75/92 (81%), Gaps = 2/92 (2%)
Frame = +1
Query: 1 VGVDQFLVKTGTITTFK--NAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKS 174
VG+DQF+ K T+T K +AH ++ MKFSVSPVVRVAV+ K +DLPKLVEGLKRLAKS
Sbjct: 455 VGLDQFITKNATLTNEKEVDAHPIRAMKFSVSPVVRVAVQCKVASDLPKLVEGLKRLAKS 514
Query: 175 DPMVQCINEESGEQIVAGAGELHLEICLRILR 270
DPMV C EESGE IVAGAGELHLEICL+ L+
Sbjct: 515 DPMVVCTMEESGEHIVAGAGELHLEICLKDLQ 546
Score = 104 bits (249), Expect = 6e-23
Identities = 50/103 (48%), Positives = 69/103 (66%), Gaps = 1/103 (0%)
Frame = +3
Query: 255 LKDLEEDH-ACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPED 431
LKDL++D I KSDPVVS+RETV + S + +SKSPNKHNRL+M+A+PM +GL E
Sbjct: 542 LKDLQDDFMGGAEIIKSDPVVSFRETVCDRSTRTVMSKSPNKHNRLYMEARPMEEGLAEA 601
Query: 432 IDEGRVNPRDDFKTRARYLTEKYEYELPKPVRFGALAPRVPAP 560
ID+GR+ PRDD K R++ L E++ ++ + A P P
Sbjct: 602 IDDGRIGPRDDPKIRSKILAEEFGWDKDLAKKIWAFGPETTGP 644
Score = 81.8 bits (193), Expect = 4e-16
Identities = 35/43 (81%), Positives = 39/43 (90%)
Frame = +2
Query: 518 ARKIWCFGPEGTGPNILVDCSKGVQYLNEIKDSVVAGFQWAAK 646
A+KIW FGPE TGPN++VD KGVQYLNEIKDSVVAGFQWA+K
Sbjct: 631 AKKIWAFGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASK 673
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 82.6 bits (195), Expect = 2e-16
Identities = 35/89 (39%), Positives = 61/89 (68%)
Frame = +1
Query: 4 GVDQFLVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPM 183
G+ ++ K+ T+++ +N + M+F VSP +RVA+EP +PAD+ L++GL+ L ++DP
Sbjct: 508 GLGPYISKSATLSSTRNCWPLASMEFQVSPTLRVAIEPSDPADMSALMKGLRLLNRADPF 567
Query: 184 VQCINEESGEQIVAGAGELHLEICLRILR 270
V+ GE ++A AGE+HLE C++ L+
Sbjct: 568 VEITVSARGEHVLAAAGEVHLERCVKDLK 596
Score = 31.9 bits (69), Expect = 0.38
Identities = 14/33 (42%), Positives = 22/33 (66%)
Frame = +3
Query: 255 LKDLEEDHACIPIKKSDPVVSYRETVAEESDQL 353
+KDL+E A + ++ S P+VSYRET+ + L
Sbjct: 592 VKDLKERFAKVNLEVSPPLVSYRETIEGDGSNL 624
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 79.8 bits (188), Expect = 1e-15
Identities = 38/92 (41%), Positives = 61/92 (66%), Gaps = 3/92 (3%)
Frame = +1
Query: 4 GVDQFLVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKS 174
GVD ++KT T+ ++ + + +KF+ PVV+ A EP NP++LPK+VEGL++++KS
Sbjct: 554 GVDASIMKTATLCNASYDEDVYIFRALKFNTLPVVKTATEPLNPSELPKMVEGLRKISKS 613
Query: 175 DPMVQCINEESGEQIVAGAGELHLEICLRILR 270
P+ EESGE + G GEL+L+ ++ LR
Sbjct: 614 YPLAITKVEESGEHTILGTGELYLDSIIKDLR 645
Score = 70.9 bits (166), Expect = 7e-13
Identities = 34/87 (39%), Positives = 55/87 (63%)
Frame = +3
Query: 252 LLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPED 431
++KDL E ++ + +K +DPVVS+ ETV E S C +++PNK N+L M A+P+ GL ED
Sbjct: 640 IIKDLRELYSEVQVKVADPVVSFCETVVESSSMKCFAETPNKKNKLTMIAEPLDRGLAED 699
Query: 432 IDEGRVNPRDDFKTRARYLTEKYEYEL 512
I+ G V+ + + KY+++L
Sbjct: 700 IENGVVSIDWNRVQLGDFFRTKYDWDL 726
Score = 45.2 bits (102), Expect = 4e-05
Identities = 21/47 (44%), Positives = 29/47 (61%), Gaps = 4/47 (8%)
Frame = +2
Query: 518 ARKIWCFGPEGTGPNILVDCSKGVQ----YLNEIKDSVVAGFQWAAK 646
AR IW FGP+ G NIL+D + + + +KDS+V GFQW A+
Sbjct: 729 ARSIWAFGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGAR 775
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 78.2 bits (184), Expect = 4e-15
Identities = 37/92 (40%), Positives = 61/92 (66%), Gaps = 3/92 (3%)
Frame = +1
Query: 4 GVDQFLVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKS 174
GVD ++KT T+ ++ + + ++F+ PVV+ A EP NP++LPK+VEGL++++KS
Sbjct: 568 GVDASIMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKS 627
Query: 175 DPMVQCINEESGEQIVAGAGELHLEICLRILR 270
P+ EESGE + G GEL+L+ ++ LR
Sbjct: 628 YPLAITKVEESGEHTILGTGELYLDSIMKDLR 659
Score = 73.7 bits (173), Expect = 9e-14
Identities = 37/103 (35%), Positives = 59/103 (57%)
Frame = +3
Query: 252 LLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPED 431
++KDL E ++ + +K +DPVVS+ ETV E S C +++PNK N++ M A+P+ GL ED
Sbjct: 654 IMKDLRELYSEVEVKVADPVVSFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAED 713
Query: 432 IDEGRVNPRDDFKTRARYLTEKYEYELPKPVRFGALAPRVPAP 560
I+ G V+ + K + KY+++L A P P
Sbjct: 714 IENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGP 756
Score = 48.8 bits (111), Expect = 3e-06
Identities = 22/47 (46%), Positives = 30/47 (63%), Gaps = 4/47 (8%)
Frame = +2
Query: 518 ARKIWCFGPEGTGPNILVDCSKGVQ----YLNEIKDSVVAGFQWAAK 646
AR IW FGP+ GPNIL+D + + + +KDS+V GFQW A+
Sbjct: 743 ARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAR 789
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 78.2 bits (184), Expect = 4e-15
Identities = 37/92 (40%), Positives = 61/92 (66%), Gaps = 3/92 (3%)
Frame = +1
Query: 4 GVDQFLVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKS 174
GVD ++KT T+ ++ + + ++F+ PVV+ A EP NP++LPK+VEGL++++KS
Sbjct: 568 GVDASIMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKS 627
Query: 175 DPMVQCINEESGEQIVAGAGELHLEICLRILR 270
P+ EESGE + G GEL+L+ ++ LR
Sbjct: 628 YPLAITKVEESGEHTILGTGELYLDSIMKDLR 659
Score = 73.7 bits (173), Expect = 9e-14
Identities = 37/103 (35%), Positives = 59/103 (57%)
Frame = +3
Query: 252 LLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPED 431
++KDL E ++ + +K +DPVVS+ ETV E S C +++PNK N++ M A+P+ GL ED
Sbjct: 654 IMKDLRELYSEVEVKVADPVVSFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAED 713
Query: 432 IDEGRVNPRDDFKTRARYLTEKYEYELPKPVRFGALAPRVPAP 560
I+ G V+ + K + KY+++L A P P
Sbjct: 714 IENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGP 756
Score = 48.8 bits (111), Expect = 3e-06
Identities = 22/47 (46%), Positives = 30/47 (63%), Gaps = 4/47 (8%)
Frame = +2
Query: 518 ARKIWCFGPEGTGPNILVDCSKGVQ----YLNEIKDSVVAGFQWAAK 646
AR IW FGP+ GPNIL+D + + + +KDS+V GFQW A+
Sbjct: 743 ARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAR 789
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 59.7 bits (138), Expect = 2e-09
Identities = 32/81 (39%), Positives = 50/81 (61%), Gaps = 1/81 (1%)
Frame = +1
Query: 34 TITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ-CINEESG 210
T++ +N ++ M F PV++VA+EPK AD+ K+ GL +LA+ DP +EE
Sbjct: 475 TLSDPENPVVLERMDFP-DPVIKVAIEPKTKADIDKMATGLIKLAQEDPSFHFSRDEEMN 533
Query: 211 EQIVAGAGELHLEICLRILRR 273
+ ++ G GELHLEI + L+R
Sbjct: 534 QTVIEGMGELHLEIIVDRLKR 554
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 46.0 bits (104), Expect = 2e-05
Identities = 23/62 (37%), Positives = 38/62 (61%), Gaps = 1/62 (1%)
Frame = +1
Query: 91 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEQIVAGAGELHLEICLRIL 267
PV+ +AV+P + + + L R K DP + ++ ESG+ I++G GELHL+I + +
Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERM 520
Query: 268 RR 273
RR
Sbjct: 521 RR 522
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 46.0 bits (104), Expect = 2e-05
Identities = 23/62 (37%), Positives = 38/62 (61%), Gaps = 1/62 (1%)
Frame = +1
Query: 91 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEQIVAGAGELHLEICLRIL 267
PV+ +AV+P + + + L R K DP + ++ ESG+ I++G GELHL+I + +
Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERM 520
Query: 268 RR 273
RR
Sbjct: 521 RR 522
>At5g41090.1 68418.m04995 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
similar to unknown protein (emb|CAB88055.1)
Length = 212
Score = 31.5 bits (68), Expect = 0.50
Identities = 18/58 (31%), Positives = 27/58 (46%)
Frame = -3
Query: 603 SLRYWTPLEQSTRMLGPVPSGPKHQILRASVTHIRTFL*DTERES*SHRGDSRDPHQC 430
S W P + T +L P +G + ++ S T I + E ES + GD +P QC
Sbjct: 80 SYNLWIPTGEKTDVLDPKKNGGELVGIKHSFTFIEN---EEEEESDNKNGDEEEPPQC 134
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 49 KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGL 156
+N N S+S +VR + K P D+PKL+E L
Sbjct: 218 ENDSNKLSSPSSLSTLVRAVLSDKKPEDVPKLIESL 253
>At5g26210.1 68418.m03119 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 255
Score = 29.9 bits (64), Expect = 1.5
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +3
Query: 339 ESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDI 434
E + LCL PN+H + + A+ +P LPE +
Sbjct: 44 EKENLCLYGHPNEHWEVNLPAEEVPPELPEPV 75
>At3g42790.1 68416.m04474 PHD finger family protein contains
PHD-finger domain, INTERPRO:IPR001965
Length = 250
Score = 27.9 bits (59), Expect = 6.1
Identities = 15/60 (25%), Positives = 27/60 (45%)
Frame = +3
Query: 249 DLLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPE 428
++ KD + I + V + + E + LCL PN+ + + A+ +P LPE
Sbjct: 15 EVFKDFKGRRTAIVKALTTDVQEFYQQCDPEKENLCLYGLPNEEWEVNLPAEEVPPELPE 74
>At1g55540.1 68414.m06356 proline-rich family protein contains
proline rich extensin domain, INTERPRO:IPR002965
Length = 915
Score = 27.9 bits (59), Expect = 6.1
Identities = 16/30 (53%), Positives = 18/30 (60%)
Frame = -2
Query: 595 VLDSFGAIHQDVGAGTLGAKAPNLTGFGNS 506
VL SFG Q +GAG GA + TGFG S
Sbjct: 749 VLGSFGQSRQ-IGAGLPGATFGSPTGFGGS 777
>At5g38500.1 68418.m04654 hypothetical protein contains Pfam profile
PF03754: Domain of unknown function (DUF313)
Length = 411
Score = 27.5 bits (58), Expect = 8.1
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Frame = +3
Query: 267 EEDHACIPIKKSDPVVSYRETVAEESD--QLCLSKSPNKHNRLFMKAQPMPDGL 422
EE + +K + S ++TV+EE D + LS P K +K Q P+G+
Sbjct: 25 EEQRRRLLAEKREDSKSQKKTVSEEDDSEKRFLSHVPRKKRSSLVKRQQKPNGV 78
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 27.5 bits (58), Expect = 8.1
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +1
Query: 100 RVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEQIVAGAGELHLEICLRIL 267
+VA+ +P D+ K+ E L RLA D + +S E + G + E L L
Sbjct: 128 KVALISHSPGDIIKVGETLVRLAVEDSQDSLLTTDSSEIVTLGGSKQGTENLLGAL 183
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 27.5 bits (58), Expect = 8.1
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +1
Query: 100 RVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEQIVAGAGELHLEICLRIL 267
+VA+ +P D+ K+ E L RLA D + +S E + G + E L L
Sbjct: 128 KVALISHSPGDIIKVGETLVRLAVEDSQDSLLTTDSSEIVTLGGSKQGTENLLGAL 183
>At2g46240.1 68415.m05750 IQ domain-containing protein / BAG
domain-containing protein contains Pfam profiles
PF00612: IQ calmodulin-binding motif, PF02179: BAG
(Apoptosis regulator Bcl-2 protein) domain
Length = 1043
Score = 27.5 bits (58), Expect = 8.1
Identities = 22/64 (34%), Positives = 32/64 (50%), Gaps = 6/64 (9%)
Frame = +1
Query: 91 PVVRVAVEPKN-PADL-----PKLVEGLKRLAKSDPMVQCINEESGEQIVAGAGELHLEI 252
PV EP+N P L PK E KR+AK++P+ E+S + A +L +
Sbjct: 400 PVANHLQEPRNIPVKLSENHLPKPTEPTKRIAKNEPVKSTKKEQSSSS--SEASKLP-PV 456
Query: 253 CLRI 264
CLR+
Sbjct: 457 CLRV 460
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,302,820
Number of Sequences: 28952
Number of extensions: 339451
Number of successful extensions: 1012
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 969
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1005
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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