Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0646
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g39280.1 68415.m04823 RabGAP/TBC domain-containing protein co... 39 0.002
At3g55020.1 68416.m06110 RabGAP/TBC domain-containing protein lo... 35 0.036
At2g01750.1 68415.m00104 expressed protein 33 0.11
At2g12520.1 68415.m01354 hypothetical protein low similarity to ... 32 0.33
At3g47460.1 68416.m05161 SMC2-like condensin, putative similar t... 31 0.77
At4g25160.1 68417.m03622 protein kinase family protein contains ... 30 1.0
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 30 1.3
At1g49040.2 68414.m05499 stomatal cytokinesis defective / SCD1 p... 29 1.8
At1g49040.1 68414.m05498 stomatal cytokinesis defective / SCD1 p... 29 1.8
At5g01850.1 68418.m00104 protein kinase, putative similar to pro... 29 3.1
At5g43130.1 68418.m05265 transcription initiation factor IID (TF... 28 4.1
At5g16210.1 68418.m01894 HEAT repeat-containing protein contains... 28 4.1
At1g13220.2 68414.m01534 nuclear matrix constituent protein-rela... 28 4.1
At1g17070.1 68414.m02077 D111/G-patch domain-containing protein ... 28 5.4
At5g61550.1 68418.m07724 protein kinase family protein contains ... 27 7.2
At4g36520.1 68417.m05185 trichohyalin-related low similarity to ... 27 7.2
At4g00650.1 68417.m00089 FRIGIDA protein identical to Swiss-Prot... 27 7.2
At2g05940.1 68415.m00645 protein kinase, putative similar to aux... 27 7.2
At4g18130.1 68417.m02695 phytochrome E (PHYE) identical to SP|P4... 27 9.5
At1g02740.1 68414.m00227 MRG family protein member of Pfam PF057... 27 9.5
>At2g39280.1 68415.m04823 RabGAP/TBC domain-containing protein
contains Pfam profile PF00566: TBC domain
Length = 771
Score = 39.1 bits (87), Expect = 0.002
Identities = 20/77 (25%), Positives = 44/77 (57%)
Frame = +3
Query: 315 RLQVEVEELRRQLTDKQELLAAAAEAIDVLEQQGSVSTDSMEVSMNNSASMKXAADPHDE 494
+L+ V ELRR ++DK+E +A + + +EQ+ V+ D+ ++ ++A+ + AA+ E
Sbjct: 637 QLEQGVTELRRLVSDKREQESAMIQVLMRMEQEHKVTEDARRLAEQDAATQRYAAEVLQE 696
Query: 495 TTEQRTPSTIRVRNRSL 545
E+ + + R++
Sbjct: 697 KYEEAVAALAEMEERAV 713
>At3g55020.1 68416.m06110 RabGAP/TBC domain-containing protein low
similarity to SP|Q9BXI6 EBP50-PDZ interactor of 64 kDa
(EPI64 protein) {Homo sapiens}; contains Pfam profile
PF00566: TBC domain
Length = 777
Score = 35.1 bits (77), Expect = 0.036
Identities = 19/77 (24%), Positives = 41/77 (53%)
Frame = +3
Query: 315 RLQVEVEELRRQLTDKQELLAAAAEAIDVLEQQGSVSTDSMEVSMNNSASMKXAADPHDE 494
+L+ E+ E++R L+DKQE A + + +EQ+ V+ D+ + ++ + + AA E
Sbjct: 651 QLEQEMAEVQRLLSDKQEQEGAMLQVLMRVEQEQKVTEDARIFAEQDAEAQRYAAQVLQE 710
Query: 495 TTEQRTPSTIRVRNRSL 545
E+ + + R++
Sbjct: 711 KYEEAVAALAEMEKRAV 727
>At2g01750.1 68415.m00104 expressed protein
Length = 629
Score = 33.5 bits (73), Expect = 0.11
Identities = 23/62 (37%), Positives = 35/62 (56%)
Frame = +3
Query: 258 EKLGNGSPPAVQGLLEHNVRLQVEVEELRRQLTDKQELLAAAAEAIDVLEQQGSVSTDSM 437
EK + +V G+L H++ LQ EV LR+ DK + L EAI++L ++ T +M
Sbjct: 508 EKAAGTNNDSVPGVL-HDL-LQKEVITLRKAANDKDQSLRDKDEAIEMLAKKVETLTKAM 565
Query: 438 EV 443
EV
Sbjct: 566 EV 567
>At2g12520.1 68415.m01354 hypothetical protein low similarity to
protective antigen [Streptococcus pyogenes] GI:8996050,
paramyosin [Anisakis simplex] GI:8117843
Length = 356
Score = 31.9 bits (69), Expect = 0.33
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = +3
Query: 303 EHNVRLQVEVEELRRQLTDKQELLAAAAEAIDVLEQQ-GSVSTD 431
EH V Q E+E L+ +L+ ++L AE I ++E + G + D
Sbjct: 135 EHEVSFQAEIERLKMELSTSKDLEKGYAEKIGLMEMEIGGLQVD 178
>At3g47460.1 68416.m05161 SMC2-like condensin, putative similar to
SMC2-like condensin (TITAN3) [Arabidopsis thaliana]
GI:14279543; contains Pfam profiles PF02483: SMC family
C-terminal domain, PF02463: RecF/RecN/SMC N terminal
domain
Length = 1171
Score = 30.7 bits (66), Expect = 0.77
Identities = 12/32 (37%), Positives = 22/32 (68%)
Frame = +3
Query: 315 RLQVEVEELRRQLTDKQELLAAAAEAIDVLEQ 410
+L+ EVEE+R Q+ +K+ L + A+ + LE+
Sbjct: 742 KLEEEVEEMRSQIKEKEGLYKSCADTVSTLEK 773
>At4g25160.1 68417.m03622 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 835
Score = 30.3 bits (65), Expect = 1.0
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +3
Query: 291 QGLLEHNVRLQVEVEELRRQLTDKQELLAAA-AEAIDVLEQQGSVSTDSME 440
Q ++ V L EVE+LR +L QE+ A A E D + G ++ +E
Sbjct: 340 QSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGELNQRRLE 390
>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
putative / HSC70, putative / HSP70, putative strong
similarity to heat shock cognate 70 kd protein 1
SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
Length = 617
Score = 29.9 bits (64), Expect = 1.3
Identities = 18/101 (17%), Positives = 43/101 (42%)
Frame = +3
Query: 207 RIAEGEFSLEIEDLFFEEKLGNGSPPAVQGLLEHNVRLQVEVEELRRQLTDKQELLAAAA 386
++ EGE + I++ + + +G PPA +G+ + V ++ + ++
Sbjct: 447 QVYEGERARTIDNNILGQFVLSGIPPAPRGIPQFTVCFDIDSNGILN--VSAEDKATGKK 504
Query: 387 EAIDVLEQQGSVSTDSMEVSMNNSASMKXAADPHDETTEQR 509
I + +G +S D +E + + K + H + E +
Sbjct: 505 NKITITNDKGRLSKDDIEKMVQEAEKYKSEDEEHKKKVEAK 545
>At1g49040.2 68414.m05499 stomatal cytokinesis defective / SCD1
protein (SCD1) contains Pfam PF02141: DENN (AEX-3)
domain; contains Pfam PF00400: WD domain, G-beta repeat
(8 copies); identical to stomatal cytokinesis defective
[Arabidopsis thaliana] GI:19743728; supporting cDNA
gi|19743727|gb|AY082605.1|; PMID 12874123
Length = 909
Score = 29.5 bits (63), Expect = 1.8
Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = +1
Query: 64 SSKHASSIPSP-FSTSPRKLQEMAMPSTEGHDVTNISGISMKQYEEQLNGLRKENFHLKL 240
+S+ IP P FST + ++ P+ D G S++Q + L+ E+ L+L
Sbjct: 310 TSEEIPQIPEPEFSTLRNDILKLLHPNVVAIDQLKGFGNSVEQCPKSLSKPWGEDHDLQL 369
Query: 241 RIYFLK 258
R+ FLK
Sbjct: 370 RVIFLK 375
>At1g49040.1 68414.m05498 stomatal cytokinesis defective / SCD1
protein (SCD1) contains Pfam PF02141: DENN (AEX-3)
domain; contains Pfam PF00400: WD domain, G-beta repeat
(8 copies); identical to stomatal cytokinesis defective
[Arabidopsis thaliana] GI:19743728; supporting cDNA
gi|19743727|gb|AY082605.1|; PMID 12874123
Length = 1187
Score = 29.5 bits (63), Expect = 1.8
Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = +1
Query: 64 SSKHASSIPSP-FSTSPRKLQEMAMPSTEGHDVTNISGISMKQYEEQLNGLRKENFHLKL 240
+S+ IP P FST + ++ P+ D G S++Q + L+ E+ L+L
Sbjct: 310 TSEEIPQIPEPEFSTLRNDILKLLHPNVVAIDQLKGFGNSVEQCPKSLSKPWGEDHDLQL 369
Query: 241 RIYFLK 258
R+ FLK
Sbjct: 370 RVIFLK 375
>At5g01850.1 68418.m00104 protein kinase, putative similar to
protein kinase [Arabidopsis thaliana]
gi|1054633|emb|CAA63387; contains protein kinase domain,
Pfam:PF00069
Length = 333
Score = 28.7 bits (61), Expect = 3.1
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2
Query: 59 RTVLNMLRVYHHRLVQVLGSCK 124
R V M RV HH LV+ +G+CK
Sbjct: 65 REVNMMSRVQHHNLVKFIGACK 86
>At5g43130.1 68418.m05265 transcription initiation factor IID
(TFIID) component TAF4 family protein weak similarity to
SP|O00268 Transcription initiation factor TFIID 135 kDa
subunit {Homo sapiens}; contains Pfam profile PF05236:
Transcription initiation factor TFIID component TAF4
family
Length = 712
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/49 (32%), Positives = 25/49 (51%)
Frame = +1
Query: 79 SSIPSPFSTSPRKLQEMAMPSTEGHDVTNISGISMKQYEEQLNGLRKEN 225
SS P P M + +DVT +SG+++++ EEQL KE+
Sbjct: 403 SSPPPPSKKQKVAGNSMDQSIEQLNDVTAVSGVNLREEEEQLFSGAKED 451
>At5g16210.1 68418.m01894 HEAT repeat-containing protein contains
Pfam profile PF02985: HEAT repeat
Length = 1180
Score = 28.3 bits (60), Expect = 4.1
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = -2
Query: 423 RLNPAALERRWPQLRPRAAPACQLIAVVTLPLLL 322
R P+A+ R L+PR A A +L + LPLLL
Sbjct: 806 RFLPSAIHPRIKGLKPRTAVANRLATLCILPLLL 839
>At1g13220.2 68414.m01534 nuclear matrix constituent protein-related
similar to nuclear matrix constituent protein 1 (NMCP1)
[Daucus carota] GI:2190187
Length = 1128
Score = 28.3 bits (60), Expect = 4.1
Identities = 8/32 (25%), Positives = 24/32 (75%)
Frame = +3
Query: 318 LQVEVEELRRQLTDKQELLAAAAEAIDVLEQQ 413
LQ E+E++R ++T K+E++ +++++ +++
Sbjct: 484 LQQEIEKIRAEMTKKEEMIEEECKSLEIKKEE 515
>At1g17070.1 68414.m02077 D111/G-patch domain-containing protein
Similar to SP|Q9ERA6 Tuftelin-interacting protein 11
{Mus musculus}; contains Pfam profile PF01585: G-patch
domain
Length = 849
Score = 27.9 bits (59), Expect = 5.4
Identities = 23/82 (28%), Positives = 39/82 (47%)
Frame = +3
Query: 237 IEDLFFEEKLGNGSPPAVQGLLEHNVRLQVEVEELRRQLTDKQELLAAAAEAIDVLEQQG 416
+E+L EEK P + L+HN+RL V++ E Q D+ L E+ L+Q+
Sbjct: 327 LENLDAEEKAKEADVPMPE--LQHNLRLIVDLVEHEIQKIDRD--LRNERESALSLQQEK 382
Query: 417 SVSTDSMEVSMNNSASMKXAAD 482
+ + E + +M+ AD
Sbjct: 383 EMLINEEEKQKRHLENMEYIAD 404
>At5g61550.1 68418.m07724 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain;
protein kinase 1, PnPK1, Populus nigra, EMBL:AB041503
Length = 845
Score = 27.5 bits (58), Expect = 7.2
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 285 AVQGLLEHNVRLQVEVEELRRQLTDKQELLAAA 383
+V + ++ V L E+E+LR +L QE+ A A
Sbjct: 348 SVHSITDNQVNLNFEIEKLRAELKHVQEMYAMA 380
>At4g36520.1 68417.m05185 trichohyalin-related low similarity to
SP|Q07283 Trichohyalin {Homo sapiens}
Length = 1400
Score = 27.5 bits (58), Expect = 7.2
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +3
Query: 300 LEHNVRLQVEVEELRRQLTDKQELLAAAAEAIDVLEQ 410
LE ++ +E EE RQ+ ++QE A +VLEQ
Sbjct: 762 LEQRLKATLEQEEKERQIKERQEREENERRAKEVLEQ 798
>At4g00650.1 68417.m00089 FRIGIDA protein identical to
Swiss-Prot:Q9FDW0 FRIGIDA protein [Arabidopsis thaliana]
Length = 318
Score = 27.5 bits (58), Expect = 7.2
Identities = 18/65 (27%), Positives = 27/65 (41%), Gaps = 1/65 (1%)
Frame = +3
Query: 231 LEIEDLFFEEKLGNGSPPAVQGLLEHNVRLQVEVEELRRQLTDKQELLAAAAEAID-VLE 407
+E E + +G P ++ V VE +RQ D Q+ + + AID LE
Sbjct: 36 VETESTSMDITIGQSKQPQFLKSIDELAAFSVAVETFKRQFDDLQKHIESIENAIDSKLE 95
Query: 408 QQGSV 422
G V
Sbjct: 96 SNGVV 100
>At2g05940.1 68415.m00645 protein kinase, putative similar to
auxin-regulated dual specificity cytosolic kinase
[Lycopersicon esculentum] gi|14484938|gb|AAK62821
Length = 462
Score = 27.5 bits (58), Expect = 7.2
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = +1
Query: 67 SKHASSIPSPFSTSPRKLQEMAMPSTEGHDVTNISGIS 180
S H S PSP + SP + STE H T +G++
Sbjct: 413 SNHPRSSPSPTTKSPSPTAKSPRNSTENHRRTLRNGVN 450
>At4g18130.1 68417.m02695 phytochrome E (PHYE) identical to SP|P42498
Phytochrome E {Arabidopsis thaliana}
Length = 1112
Score = 27.1 bits (57), Expect = 9.5
Identities = 17/46 (36%), Positives = 23/46 (50%)
Frame = +3
Query: 201 VKRIAEGEFSLEIEDLFFEEKLGNGSPPAVQGLLEHNVRLQVEVEE 338
+K I EG+ LE E+ E L + L E N +L+VEV E
Sbjct: 928 LKSIEEGKLQLETEEFRLENILDTIISQVMIILRERNSQLRVEVAE 973
>At1g02740.1 68414.m00227 MRG family protein member of Pfam PF05712:
MRG; similar to Transcription factor-like protein MRG15
(MORF-related gene 15 protein) (MSL3-1 protein) (Protein
HSPC008/HSPC061) (SP:Q9UBU8) {Homo sapiens}
Length = 316
Score = 27.1 bits (57), Expect = 9.5
Identities = 20/59 (33%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = +3
Query: 252 FEEKLGNG-SPPAVQGLLEHNVRLQVEVEELRRQLTDKQELLAAAAE-AIDVLEQQGSV 422
+EE + G SP V G EH +RL V++ EL + +E L + +D+L + SV
Sbjct: 242 YEESVSGGVSPSTVYG-AEHLLRLFVKLPELLVHVNMAEETLKELQDNFVDILRKNQSV 299
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,231,686
Number of Sequences: 28952
Number of extensions: 237561
Number of successful extensions: 903
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 882
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 903
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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