Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0643
(480 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 137 9e-34
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 87 1e-18
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 86 2e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 70 2e-13
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.5
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 27 1.9
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 2.6
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 2.6
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 2.6
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 3.4
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 137 bits (331), Expect = 9e-34
Identities = 60/75 (80%), Positives = 68/75 (90%)
Frame = +2
Query: 254 LVDLEPGTMDSVRSGPFGQIFRPDNFVFRQSGAGNNWAKGHYTEGAELVDSVLDVVRKEA 433
LVDLEPGTMD+V+SG FG +FRPDN ++ QSGAGN WAKGHYTEGAEL D+VLDVVR+EA
Sbjct: 65 LVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREA 124
Query: 434 ESCDCLQGFQLTHSL 478
E+CD LQGFQLTHSL
Sbjct: 125 EACDALQGFQLTHSL 139
Score = 113 bits (273), Expect = 1e-26
Identities = 47/64 (73%), Positives = 55/64 (85%)
Frame = +3
Query: 63 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 242
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 243 PRAI 254
PRA+
Sbjct: 61 PRAV 64
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 87.4 bits (207), Expect = 1e-18
Identities = 38/78 (48%), Positives = 55/78 (70%), Gaps = 1/78 (1%)
Frame = +2
Query: 245 PRHL-VDLEPGTMDSVRSGPFGQIFRPDNFVFRQSGAGNNWAKGHYTEGAELVDSVLDVV 421
PR + VDLEP +D VR+GP+ +F P+ V + A NN+A+GHYT G E++DSVL+ +
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 422 RKEAESCDCLQGFQLTHS 475
R+ A++C LQGF + HS
Sbjct: 127 RRMADNCSGLQGFLVFHS 144
Score = 51.2 bits (117), Expect = 8e-08
Identities = 25/70 (35%), Positives = 37/70 (52%), Gaps = 6/70 (8%)
Frame = +3
Query: 63 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 224
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 225 SGGKYVPRAI 254
GK+VPR+I
Sbjct: 61 GQGKFVPRSI 70
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 86.2 bits (204), Expect = 2e-18
Identities = 38/78 (48%), Positives = 53/78 (67%), Gaps = 1/78 (1%)
Frame = +2
Query: 245 PRHL-VDLEPGTMDSVRSGPFGQIFRPDNFVFRQSGAGNNWAKGHYTEGAELVDSVLDVV 421
PR + VDLEP +D VR+GP+ +F P+ + + A NN+A+GHYT G ELVD V D +
Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKI 122
Query: 422 RKEAESCDCLQGFQLTHS 475
R+ A++C LQGF + HS
Sbjct: 123 RRIADNCSGLQGFLVFHS 140
Score = 57.2 bits (132), Expect = 1e-09
Identities = 28/66 (42%), Positives = 38/66 (57%), Gaps = 2/66 (3%)
Frame = +3
Query: 63 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 236
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 237 YVPRAI 254
YVPR+I
Sbjct: 61 YVPRSI 66
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 70.1 bits (164), Expect = 2e-13
Identities = 28/77 (36%), Positives = 51/77 (66%), Gaps = 2/77 (2%)
Frame = +2
Query: 254 LVDLEPGTMDSVRSGPFGQIFRPDNFVFRQSG--AGNNWAKGHYTEGAELVDSVLDVVRK 427
L+DLEP ++++ S +G ++ P+N + ++G AGNNWA G Y+ + + ++D++ +
Sbjct: 66 LIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDR 124
Query: 428 EAESCDCLQGFQLTHSL 478
EA+ D L+GF L HS+
Sbjct: 125 EADGSDSLEGFSLLHSI 141
Score = 68.1 bits (159), Expect = 6e-13
Identities = 28/63 (44%), Positives = 44/63 (69%)
Frame = +3
Query: 66 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 245
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 246 RAI 254
RAI
Sbjct: 63 RAI 65
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.5
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +3
Query: 102 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 203
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 26.6 bits (56), Expect = 1.9
Identities = 20/52 (38%), Positives = 26/52 (50%), Gaps = 4/52 (7%)
Frame = -1
Query: 462 WNPWRQSHDSASFRTTSKT----ESTSSAPSV*CPLAQLLPAPDCLKTKLSG 319
++P +S D+ S RT S + TSS PS AQLL P L K +G
Sbjct: 355 FSPSYRSSDTHSPRTRSPNVQTHKKTSSQPSDLSSFAQLLSPPQVLSPKPNG 406
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 2.6
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2
Query: 365 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 454
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 2.6
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -1
Query: 438 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCLKTKLSGRKICPK 301
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.2 bits (55), Expect = 2.6
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 296 GPFGQIFRPDNFVFRQSGAG-NNWAKGHYTEGAEL 397
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 3.4
Identities = 12/44 (27%), Positives = 21/44 (47%)
Frame = +2
Query: 317 RPDNFVFRQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 448
RP +F + G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIFYDN-CGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,897,544
Number of Sequences: 5004
Number of extensions: 35640
Number of successful extensions: 112
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 101
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 184020746
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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