Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0623
(765 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 108 3e-24
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 104 7e-23
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 104 7e-23
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 104 7e-23
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 103 1e-22
>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 2 (Methionine
adenosyltransferase 2, AdoMet synthetase 2)
[Catharanthus roseus] SWISS-PROT:Q96552
Length = 393
Score = 108 bits (260), Expect = 3e-24
Identities = 54/98 (55%), Positives = 69/98 (70%), Gaps = 2/98 (2%)
Frame = +1
Query: 256 GEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RN 429
GEIT+KANVDY+++VR+T + IG+ + G D C V++ ++QQSP+IA GVH + +
Sbjct: 55 GEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKK 114
Query: 430 DEEVGAGDQGLMFGYATDETEECMPLM*CLHTNSIRKL 543
EEVGAGDQG MFGYATDET E MPL L T KL
Sbjct: 115 PEEVGAGDQGHMFGYATDETPELMPLTHVLATKLGAKL 152
Score = 97.5 bits (232), Expect = 9e-21
Identities = 45/83 (54%), Positives = 58/83 (69%)
Frame = +3
Query: 513 VLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQHSEKIT 692
VLA KL K+ E+R+NG W RPD KTQVT EY+ GA VP RVHTV++S QH E +T
Sbjct: 143 VLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYINESGAMVPVRVHTVLISTQHDETVT 202
Query: 693 LETLRDEIREKVIKEVIPAQYLD 761
+ + +++E VIK VIP +YLD
Sbjct: 203 NDEIAADLKEHVIKPVIPEKYLD 225
Score = 91.9 bits (218), Expect = 4e-19
Identities = 44/70 (62%), Positives = 53/70 (75%), Gaps = 4/70 (5%)
Frame = +2
Query: 104 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVL----LLAKSHP 271
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT MV+ + K++
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63
Query: 272 KLTWIIKKLC 301
I++K C
Sbjct: 64 DYEQIVRKTC 73
>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
identical to S-adenosylmethionine synthetase 2
(Methionine adenosyltransferase 2, AdoMet synthetase 2)
[Arabidopsis thaliana] SWISS-PROT:P17562
Length = 393
Score = 104 bits (249), Expect = 7e-23
Identities = 49/92 (53%), Positives = 65/92 (70%), Gaps = 2/92 (2%)
Frame = +1
Query: 256 GEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RN 429
GEIT+KA +DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + +
Sbjct: 55 GEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKR 114
Query: 430 DEEVGAGDQGLMFGYATDETEECMPLM*CLHT 525
E++GAGDQG MFGYATDET E MPL L T
Sbjct: 115 PEDIGAGDQGHMFGYATDETPELMPLSHVLAT 146
Score = 93.5 bits (222), Expect = 1e-19
Identities = 43/65 (66%), Positives = 51/65 (78%)
Frame = +2
Query: 104 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLLAKSHPKLTW 283
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ + K T
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATI 63
Query: 284 IIKKL 298
+K+
Sbjct: 64 DYEKI 68
Score = 93.5 bits (222), Expect = 1e-19
Identities = 42/83 (50%), Positives = 57/83 (68%)
Frame = +3
Query: 513 VLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQHSEKIT 692
VLA K+ ++ E+R+NG W RPD KTQVT EY GA VP RVHTV++S QH E +T
Sbjct: 143 VLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVT 202
Query: 693 LETLRDEIREKVIKEVIPAQYLD 761
+ + +++E VIK +IP +YLD
Sbjct: 203 NDEIARDLKEHVIKPIIPEKYLD 225
>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 104 bits (249), Expect = 7e-23
Identities = 51/92 (55%), Positives = 65/92 (70%), Gaps = 2/92 (2%)
Frame = +1
Query: 256 GEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RN 429
GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + +
Sbjct: 55 GEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKC 114
Query: 430 DEEVGAGDQGLMFGYATDETEECMPLM*CLHT 525
EE+GAGDQG MFGYATDET E MPL L T
Sbjct: 115 PEEIGAGDQGHMFGYATDETPELMPLSHVLAT 146
Score = 93.5 bits (222), Expect = 1e-19
Identities = 43/65 (66%), Positives = 51/65 (78%)
Frame = +2
Query: 104 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLLAKSHPKLTW 283
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ + K T
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63
Query: 284 IIKKL 298
+K+
Sbjct: 64 DYEKI 68
Score = 93.5 bits (222), Expect = 1e-19
Identities = 44/83 (53%), Positives = 57/83 (68%)
Frame = +3
Query: 513 VLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQHSEKIT 692
VLA KL ++ E+R+NG W RPD KTQVT EY GA VP RVHTV++S QH E +T
Sbjct: 143 VLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLISTQHDETVT 202
Query: 693 LETLRDEIREKVIKEVIPAQYLD 761
+ + +++E VIK VIP +YLD
Sbjct: 203 NDEIARDLKEHVIKPVIPEKYLD 225
>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 104 bits (249), Expect = 7e-23
Identities = 51/92 (55%), Positives = 65/92 (70%), Gaps = 2/92 (2%)
Frame = +1
Query: 256 GEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RN 429
GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + +
Sbjct: 55 GEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKC 114
Query: 430 DEEVGAGDQGLMFGYATDETEECMPLM*CLHT 525
EE+GAGDQG MFGYATDET E MPL L T
Sbjct: 115 PEEIGAGDQGHMFGYATDETPELMPLSHVLAT 146
Score = 93.5 bits (222), Expect = 1e-19
Identities = 43/65 (66%), Positives = 51/65 (78%)
Frame = +2
Query: 104 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLLAKSHPKLTW 283
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ + K T
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63
Query: 284 IIKKL 298
+K+
Sbjct: 64 DYEKI 68
Score = 93.5 bits (222), Expect = 1e-19
Identities = 44/83 (53%), Positives = 57/83 (68%)
Frame = +3
Query: 513 VLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQHSEKIT 692
VLA KL ++ E+R+NG W RPD KTQVT EY GA VP RVHTV++S QH E +T
Sbjct: 143 VLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLISTQHDETVT 202
Query: 693 LETLRDEIREKVIKEVIPAQYLD 761
+ + +++E VIK VIP +YLD
Sbjct: 203 NDEIARDLKEHVIKPVIPEKYLD 225
>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 3 (Methionine
adenosyltransferase 3, AdoMet synthetase 3)
[Lycopersicon esculentum] SWISS-PROT:P43282
Length = 390
Score = 103 bits (247), Expect = 1e-22
Identities = 51/98 (52%), Positives = 67/98 (68%), Gaps = 2/98 (2%)
Frame = +1
Query: 256 GEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RN 429
GEIT+ A VDY+K+VR T + IG+ + G D C+V++ ++QQSP+IA GVH + +
Sbjct: 55 GEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKK 114
Query: 430 DEEVGAGDQGLMFGYATDETEECMPLM*CLHTNSIRKL 543
E++GAGDQG MFGYATDET E MPL L T KL
Sbjct: 115 PEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKL 152
Score = 95.5 bits (227), Expect = 3e-20
Identities = 45/83 (54%), Positives = 58/83 (69%)
Frame = +3
Query: 513 VLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQHSEKIT 692
VLA KL K+ E+R+N W RPD KTQVT EY GGA +P RVHTV++S QH E +T
Sbjct: 143 VLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDGGAMIPIRVHTVLISTQHDETVT 202
Query: 693 LETLRDEIREKVIKEVIPAQYLD 761
+ + +++E VIK VIPA+YLD
Sbjct: 203 NDEIAADLKEHVIKPVIPAKYLD 225
Score = 90.2 bits (214), Expect = 1e-18
Identities = 40/53 (75%), Positives = 46/53 (86%)
Frame = +2
Query: 104 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLLAK 262
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT MV++ +
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGE 56
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,344,873
Number of Sequences: 28952
Number of extensions: 423032
Number of successful extensions: 1220
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 1163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1214
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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