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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0622
         (768 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g25757.1 68418.m03055 expressed protein                             94   8e-20
At5g25754.1 68418.m03054 expressed protein                             94   8e-20
At2g40000.1 68415.m04915 expressed protein                             30   1.5  
At5g16500.1 68418.m01928 protein kinase family protein contains ...    28   6.0  
At5g27280.1 68418.m03256 zinc finger (DNL type) family protein c...    28   7.9  
At3g43300.1 68416.m04570 guanine nucleotide exchange family prot...    28   7.9  

>At5g25757.1 68418.m03055 expressed protein
          Length = 514

 Score = 94.3 bits (224), Expect = 8e-20
 Identities = 38/84 (45%), Positives = 61/84 (72%)
 Frame = +2

Query: 257 EKYFENTPWPDEDEVSPIVDNDNVFMILYKELYYRDIYARVPGGPKPEQRFHSFYNYCDL 436
           E+ F++TPWP  + ++P VDND+VF +LY+E+++R +YAR+   P  +QR  S+ NYC L
Sbjct: 59  ERLFKDTPWPSVEAIAPYVDNDHVFCLLYREMWFRHLYARL--SPTLKQRIDSYDNYCSL 116

Query: 437 FNYILSVTPVPLELPDQWLWELID 508
           F  +L    V ++LP+QWLW+++D
Sbjct: 117 FQVVLHGV-VNMQLPNQWLWDMVD 139



 Score = 57.2 bits (132), Expect = 1e-08
 Identities = 28/56 (50%), Positives = 37/56 (66%)
 Frame = +1

Query: 511 FVYQFQSFAQYRSRTTKLSPAEIEALNTENKAWNVLCILNVLHSLVDKSNIKRQLE 678
           FVYQFQSF Q+R++    +  EI  L   +KAWNV  +LN L +LV+KS I + LE
Sbjct: 141 FVYQFQSFCQFRAKLKNKTEEEIALLRQHDKAWNVYGVLNFLQALVEKSCIIQILE 196



 Score = 30.7 bits (66), Expect = 1.1
 Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
 Frame = +3

Query: 96  AQSTEHYEPEYDRSSYYK---MPDMVKKFLVYFRNMINEGVTYEILNLYENTFPTLLRNT 266
           A S E+ E   +R S Y    +PD VK F+ +    I +   YEI  + E +F ++    
Sbjct: 2   ASSNEYEEGPGERESGYDPNMVPDSVKSFVSHMYRHIRDKNVYEIHQMCETSFQSISERL 61

Query: 267 LK 272
            K
Sbjct: 62  FK 63


>At5g25754.1 68418.m03054 expressed protein
          Length = 514

 Score = 94.3 bits (224), Expect = 8e-20
 Identities = 38/84 (45%), Positives = 61/84 (72%)
 Frame = +2

Query: 257 EKYFENTPWPDEDEVSPIVDNDNVFMILYKELYYRDIYARVPGGPKPEQRFHSFYNYCDL 436
           E+ F++TPWP  + ++P VDND+VF +LY+E+++R +YAR+   P  +QR  S+ NYC L
Sbjct: 59  ERLFKDTPWPSVEAIAPYVDNDHVFCLLYREMWFRHLYARL--SPTLKQRIDSYDNYCSL 116

Query: 437 FNYILSVTPVPLELPDQWLWELID 508
           F  +L    V ++LP+QWLW+++D
Sbjct: 117 FQVVLHGV-VNMQLPNQWLWDMVD 139



 Score = 57.2 bits (132), Expect = 1e-08
 Identities = 28/56 (50%), Positives = 37/56 (66%)
 Frame = +1

Query: 511 FVYQFQSFAQYRSRTTKLSPAEIEALNTENKAWNVLCILNVLHSLVDKSNIKRQLE 678
           FVYQFQSF Q+R++    +  EI  L   +KAWNV  +LN L +LV+KS I + LE
Sbjct: 141 FVYQFQSFCQFRAKLKNKTEEEIALLRQHDKAWNVYGVLNFLQALVEKSCIIQILE 196



 Score = 30.7 bits (66), Expect = 1.1
 Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
 Frame = +3

Query: 96  AQSTEHYEPEYDRSSYYK---MPDMVKKFLVYFRNMINEGVTYEILNLYENTFPTLLRNT 266
           A S E+ E   +R S Y    +PD VK F+ +    I +   YEI  + E +F ++    
Sbjct: 2   ASSNEYEEGPGERESGYDPNMVPDSVKSFVSHMYRHIRDKNVYEIHQMCETSFQSISERL 61

Query: 267 LK 272
            K
Sbjct: 62  FK 63


>At2g40000.1 68415.m04915 expressed protein
          Length = 435

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 13/40 (32%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
 Frame = +1

Query: 601 KAWNVLCILNVLHSLVDKSN---IKRQLEVYASGGDPDFC 711
           K W +L  +  LH L+D  +   +K+QL++ ++G +  FC
Sbjct: 270 KIWKILAEIEDLHMLMDPEDFLKLKKQLQIKSTGKNDAFC 309


>At5g16500.1 68418.m01928 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 636

 Score = 28.3 bits (60), Expect = 6.0
 Identities = 12/32 (37%), Positives = 17/32 (53%)
 Frame = +3

Query: 36  TMYSSDDYNEGGYESYGDYEAQSTEHYEPEYD 131
           ++YS DD  E G  S    EA+  EH   ++D
Sbjct: 605 SVYSDDDAGESGESSLHRIEAKEEEHISSDHD 636


>At5g27280.1 68418.m03256 zinc finger (DNL type) family protein
           contains Pfam profile PF05180: DNL zinc finger
          Length = 212

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 12/39 (30%), Positives = 19/39 (48%)
 Frame = +1

Query: 592 TENKAWNVLCILNVLHSLVDKSNIKRQLEVYASGGDPDF 708
           T+   +   C  NV H LVD  N+  +++ Y S    D+
Sbjct: 144 TDGTVFVQCCGCNVFHKLVDNLNLFHEVKYYVSSSSFDY 182


>At3g43300.1 68416.m04570 guanine nucleotide exchange family protein
           similar to SP|Q9Y6D5 Brefeldin A-inhibited guanine
           nucleotide-exchange protein 2 {Homo sapiens}; contains
           Pfam profile PF01369: Sec7 domain
          Length = 1756

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 12/26 (46%), Positives = 18/26 (69%)
 Frame = +1

Query: 601 KAWNVLCILNVLHSLVDKSNIKRQLE 678
           K  ++ C++NVL SLVD   I+R+ E
Sbjct: 536 KGSSLQCLVNVLKSLVDWEKIRREAE 561


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,721,084
Number of Sequences: 28952
Number of extensions: 356833
Number of successful extensions: 1143
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1086
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1141
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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