Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0619
(724 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 124 1e-29
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 82 7e-17
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 81 2e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 72 7e-14
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 29 0.51
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.7
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.6
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.7
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 4.7
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 6.3
SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|ch... 25 8.3
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 124 bits (300), Expect = 1e-29
Identities = 53/67 (79%), Positives = 61/67 (91%)
Frame = +1
Query: 256 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESC 435
LEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+EAE+C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 436 DCLQGFQ 456
D LQGFQ
Sbjct: 128 DALQGFQ 134
Score = 118 bits (284), Expect = 8e-28
Identities = 50/70 (71%), Positives = 58/70 (82%)
Frame = +2
Query: 56 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 235
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 236 PRAILVAWSP 265
PRA+LV P
Sbjct: 61 PRAVLVDLEP 70
Score = 115 bits (277), Expect = 6e-27
Identities = 52/70 (74%), Positives = 61/70 (87%)
Frame = +3
Query: 510 SKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICF 689
SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I
Sbjct: 153 SKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFA 212
Query: 690 RTLKLSTPTY 719
TLK+ +P+Y
Sbjct: 213 NTLKIKSPSY 222
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 82.2 bits (194), Expect = 7e-17
Identities = 33/66 (50%), Positives = 48/66 (72%)
Frame = +1
Query: 256 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESC 435
LEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ A++C
Sbjct: 74 LEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNC 133
Query: 436 DCLQGF 453
LQGF
Sbjct: 134 SGLQGF 139
Score = 75.4 bits (177), Expect = 8e-15
Identities = 32/69 (46%), Positives = 47/69 (68%)
Frame = +3
Query: 513 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 692
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219
Query: 693 TLKLSTPTY 719
L + PTY
Sbjct: 220 NLDIERPTY 228
Score = 53.6 bits (123), Expect = 3e-08
Identities = 27/76 (35%), Positives = 39/76 (51%), Gaps = 6/76 (7%)
Frame = +2
Query: 56 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 217
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 218 SGGKYVPRAILVAWSP 265
GK+VPR+I V P
Sbjct: 61 GQGKFVPRSIYVDLEP 76
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 81.0 bits (191), Expect = 2e-16
Identities = 33/66 (50%), Positives = 46/66 (69%)
Frame = +1
Query: 256 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESC 435
LEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ A++C
Sbjct: 70 LEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNC 129
Query: 436 DCLQGF 453
LQGF
Sbjct: 130 SGLQGF 135
Score = 68.9 bits (161), Expect = 7e-13
Identities = 29/69 (42%), Positives = 45/69 (65%)
Frame = +3
Query: 513 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 692
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
Query: 693 TLKLSTPTY 719
L + P+Y
Sbjct: 216 NLDIERPSY 224
Score = 59.7 bits (138), Expect = 4e-10
Identities = 30/72 (41%), Positives = 40/72 (55%), Gaps = 2/72 (2%)
Frame = +2
Query: 56 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 229
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 230 YVPRAILVAWSP 265
YVPR+I V P
Sbjct: 61 YVPRSIYVDLEP 72
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 72.1 bits (169), Expect = 7e-14
Identities = 30/69 (43%), Positives = 47/69 (68%)
Frame = +2
Query: 59 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 238
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 239 RAILVAWSP 265
RAIL+ P
Sbjct: 63 RAILIDLEP 71
Score = 60.1 bits (139), Expect = 3e-10
Identities = 29/70 (41%), Positives = 41/70 (58%), Gaps = 1/70 (1%)
Frame = +3
Query: 513 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICF 689
++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAA 215
Query: 690 RTLKLSTPTY 719
L PT+
Sbjct: 216 DRLHTQNPTF 225
Score = 57.6 bits (133), Expect = 2e-09
Identities = 23/68 (33%), Positives = 44/68 (64%), Gaps = 2/68 (2%)
Frame = +1
Query: 256 LEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKEAE 429
LEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +EA+
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 430 SCDCLQGF 453
D L+GF
Sbjct: 128 GSDSLEGF 135
>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 29.5 bits (63), Expect = 0.51
Identities = 24/70 (34%), Positives = 38/70 (54%), Gaps = 11/70 (15%)
Frame = +3
Query: 486 FRYGHPPHSKIREEYP-DRIMNTYSVVP--SPKVSDTVVE---PYN--ATLSVHQL---V 632
++Y H SK++ EYP D+++ SV+ PK VVE P N A L+ H + +
Sbjct: 391 YQYAHVVASKLQREYPRDKVLYYSSVLTEFDPKGIQEVVESLRPNNFFAILAAHSIEKGL 450
Query: 633 ENTDETYCID 662
+N ++ Y ID
Sbjct: 451 DNKEKFYGID 460
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.7
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 95 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 196
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.6
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 289 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 390
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 358 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 447
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 4.7
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -3
Query: 431 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 294
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 6.3
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 310 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 441
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 783
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/38 (28%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +3
Query: 492 YGHPPHSKIREEYP-DRIMNTYSVVPSPKVSDTVVEPY 602
Y H HS E+ + + S++P P+VSD +++ +
Sbjct: 231 YSHTHHSYPPNEHDVNAVQKALSLLPPPEVSDFLIKSF 268
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,765,943
Number of Sequences: 5004
Number of extensions: 52923
Number of successful extensions: 185
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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