Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0617
(709 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 52 6e-08
SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual 32 0.092
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 29 0.65
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 29 0.65
SPBC28F2.03 |ppi1|cyp2|cyclophilin family peptidyl-prolyl cis-tr... 27 2.0
SPAC637.10c |rpn10|pus1|19S proteasome regulatory subunit Rpn10|... 27 2.0
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 2.6
SPBC418.02 |||NatA N-acetyltransferase complex subunit |Schizosa... 27 3.5
SPBC27.08c |sua1|SPBC28F2.01c|sulfate adenylyltransferase |Schiz... 26 4.6
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 52.4 bits (120), Expect = 6e-08
Identities = 23/63 (36%), Positives = 41/63 (65%)
Frame = +1
Query: 67 EFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 246
+ AE A +GP+ + LA KY + I+ E++EK S+I++N+ + I++ GN+ G +RK
Sbjct: 59 QIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEKQSNIIYNSCIYITENGNLGGVYRK 118
Query: 247 NHI 255
H+
Sbjct: 119 VHL 121
Score = 28.3 bits (60), Expect = 1.1
Identities = 18/80 (22%), Positives = 35/80 (43%), Gaps = 2/80 (2%)
Frame = +3
Query: 270 FNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEG 449
F+ + + + P+F T +GK+ V IC+ + NGA+++ +A
Sbjct: 122 FDTERKHFKKGSDFPIFETSFGKLGVMICWDTAFPEVARIHALNGADLL-----VVATNW 176
Query: 450 GSEYM--WNVEARNAAITNC 503
+ Y W++ + A NC
Sbjct: 177 ENPYSDDWDLVTKARAFENC 196
Score = 25.8 bits (54), Expect = 6.1
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +2
Query: 599 FYGSSYFCGPDGVKCPGLSRTRDGLLIAAVDLN 697
F+G S GP G L ++G++ VDL+
Sbjct: 213 FFGHSKIIGPTGKVIKALDEEKEGVISYTVDLD 245
>SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 276
Score = 31.9 bits (69), Expect = 0.092
Identities = 24/79 (30%), Positives = 32/79 (40%), Gaps = 1/79 (1%)
Frame = +3
Query: 276 ESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEGGS 455
ESN + G P T GK+ ICF + GA I+ PSA G +
Sbjct: 130 ESNTTLRGEAILPPCKTPLGKVGSAICFDIRFPEQAIKLRNMGAHIITYPSAFTEKTGAA 189
Query: 456 EYMWNVEARNAAI-TNCYL 509
W V R A+ + CY+
Sbjct: 190 H--WEVLLRARALDSQCYV 206
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 29.1 bits (62), Expect = 0.65
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +1
Query: 169 EKHSDILWNTAVVISDTGNVIGKHRKNHI 255
E+ L+NTA+V +G +I HRK H+
Sbjct: 129 ERKDGKLYNTAMVFDPSGKLIAVHRKIHL 157
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 29.1 bits (62), Expect = 0.65
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = +1
Query: 79 SAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDIL 189
SAE+ + + + +++VS++LE DEKH D++
Sbjct: 979 SAENTTSFSIFAAQGLTDFLIVVSNLLEMDEKHVDVV 1015
>SPBC28F2.03 |ppi1|cyp2|cyclophilin family peptidyl-prolyl cis-trans
isomerase Cyp2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 162
Score = 27.5 bits (58), Expect = 2.0
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = -3
Query: 374 DVVPSEADVHRDLAVSGRKYRMAGVTFHVVV 282
DVVP A R L + Y AG TFH V+
Sbjct: 25 DVVPKTAANFRALCTGEKGYGYAGSTFHRVI 55
>SPAC637.10c |rpn10|pus1|19S proteasome regulatory subunit
Rpn10|Schizosaccharomyces pombe|chr 1|||Manual
Length = 243
Score = 27.5 bits (58), Expect = 2.0
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = -1
Query: 499 FVIAAFLASTFHMYSLPPSPAIVAD 425
F+ AA + + H+ S+PPSP +++D
Sbjct: 156 FIDAANSSDSCHLVSIPPSPQLLSD 180
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.1 bits (57), Expect = 2.6
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -1
Query: 382 QFKTWCRPKQMFTAILPYLVANTGWPVLPS 293
+F T K +FTA+ P+L A T + ++PS
Sbjct: 1521 RFPTRVLCKPVFTAVPPFLFAGTDFALIPS 1550
>SPBC418.02 |||NatA N-acetyltransferase complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/39 (30%), Positives = 21/39 (53%), Gaps = 2/39 (5%)
Frame = +3
Query: 252 HPRVGDFNESNYYMEGNTGH-PVFATRY-GKIAVNICFG 362
H ++GD+ ESNY++ H P + + K + +C G
Sbjct: 375 HYKLGDYEESNYWLNLAIDHTPTYPELFLAKAKIFLCMG 413
>SPBC27.08c |sua1|SPBC28F2.01c|sulfate adenylyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 490
Score = 26.2 bits (55), Expect = 4.6
Identities = 13/29 (44%), Positives = 14/29 (48%)
Frame = -3
Query: 347 HRDLAVSGRKYRMAGVTFHVVVGFVKIAD 261
HR+L V K A V H VVG K D
Sbjct: 202 HRELTVRAAKQHGARVLIHPVVGMTKPGD 230
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,818,253
Number of Sequences: 5004
Number of extensions: 58140
Number of successful extensions: 178
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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