Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0617
(709 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 140 1e-33
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 40 0.002
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 40 0.002
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 40 0.002
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 40 0.002
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 33 0.25
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 32 0.43
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 30 1.3
At5g67240.1 68418.m08475 exonuclease family protein contains exo... 29 3.0
At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family prot... 29 3.0
At5g06970.1 68418.m00789 expressed protein 29 4.0
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 28 5.3
At2g44760.1 68415.m05571 expressed protein 28 7.0
At2g39980.1 68415.m04913 transferase family protein contains Pfa... 28 7.0
At3g48580.1 68416.m05304 xyloglucan:xyloglucosyl transferase, pu... 27 9.2
At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N domain-... 27 9.2
At1g10190.1 68414.m01149 expressed protein similar to hypothetic... 27 9.2
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 140 bits (338), Expect = 1e-33
Identities = 65/84 (77%), Positives = 71/84 (84%)
Frame = +3
Query: 255 PRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQNGAEIVFNPSAT 434
PRVGDFNES YYMEG+TGHPVF T +GKIAVNIC+GRHH LNW+ FG NGAEIVFNPSAT
Sbjct: 216 PRVGDFNESTYYMEGDTGHPVFETVFGKIAVNICYGRHHPLNWLAFGLNGAEIVFNPSAT 275
Query: 435 IAGEGGSEYMWNVEARNAAITNCY 506
+ GE SE MW +EARNAAI N Y
Sbjct: 276 V-GE-LSEPMWPIEARNAAIANSY 297
Score = 129 bits (311), Expect = 2e-30
Identities = 58/85 (68%), Positives = 68/85 (80%)
Frame = +1
Query: 1 ICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHS 180
+C QE W MPFAFCTRE++ WCEFAE DG +T FL+ELA KY MVIVS ILERD H
Sbjct: 133 LCLQEAWTMPFAFCTRERR-WCEFAEPV-DGESTKFLQELAKKYNMVIVSPILERDIDHG 190
Query: 181 DILWNTAVVISDTGNVIGKHRKNHI 255
++LWNTAV+I + GN+IGKHRKNHI
Sbjct: 191 EVLWNTAVIIGNNGNIIGKHRKNHI 215
Score = 89.4 bits (212), Expect = 2e-18
Identities = 42/65 (64%), Positives = 47/65 (72%)
Frame = +2
Query: 515 AINRVGYEEFPNEFTSADGKPAHKDLGLFYGSSYFCGPDGVKCPGLSRTRDGLLIAAVDL 694
+INRVG E FPN FTS DGKP H D G FYGSS+F PD P LSR +DGLLI+ +DL
Sbjct: 301 SINRVGTEVFPNPFTSGDGKPQHNDFGHFYGSSHFSAPDASCTPSLSRYKDGLLISDMDL 360
Query: 695 NLNRQ 709
NL RQ
Sbjct: 361 NLCRQ 365
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 39.5 bits (88), Expect = 0.002
Identities = 24/87 (27%), Positives = 43/87 (49%), Gaps = 2/87 (2%)
Frame = +1
Query: 1 ICFQELWNMPFAFCTREKQP-WCEFAESAEDG-PTTTFLRELAIKYAMVIVSSILERDEK 174
+ E+WN P++ + P + E ++ D P+T L E++ + + I+ + E+
Sbjct: 123 VLLPEIWNSPYS---NDSFPVYAEEIDAGGDASPSTAMLSEVSKRLKITIIGGSIP--ER 177
Query: 175 HSDILWNTAVVISDTGNVIGKHRKNHI 255
D L+NT V G + KHRK H+
Sbjct: 178 VGDRLYNTCCVFGSDGELKAKHRKIHL 204
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 39.5 bits (88), Expect = 0.002
Identities = 24/87 (27%), Positives = 43/87 (49%), Gaps = 2/87 (2%)
Frame = +1
Query: 1 ICFQELWNMPFAFCTREKQP-WCEFAESAEDG-PTTTFLRELAIKYAMVIVSSILERDEK 174
+ E+WN P++ + P + E ++ D P+T L E++ + + I+ + E+
Sbjct: 123 VLLPEIWNSPYS---NDSFPVYAEEIDAGGDASPSTAMLSEVSKRLKITIIGGSIP--ER 177
Query: 175 HSDILWNTAVVISDTGNVIGKHRKNHI 255
D L+NT V G + KHRK H+
Sbjct: 178 VGDRLYNTCCVFGSDGELKAKHRKIHL 204
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 39.5 bits (88), Expect = 0.002
Identities = 20/54 (37%), Positives = 29/54 (53%)
Frame = +3
Query: 270 FNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQNGAEIVFNPSA 431
+ E Y+ G+TG VF T++ KI V IC+ + GAEI+F P+A
Sbjct: 158 YQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTA 211
Score = 36.3 bits (80), Expect = 0.020
Identities = 25/86 (29%), Positives = 47/86 (54%), Gaps = 1/86 (1%)
Frame = +1
Query: 1 ICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVI-VSSILERDEKH 177
I QEL+ + FC +++ + + A+ ++ PT +++LA + +VI VS E + H
Sbjct: 72 ILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVIPVSFFEEANTAH 130
Query: 178 SDILWNTAVVISDTGNVIGKHRKNHI 255
+N+ +I G +G +RK+HI
Sbjct: 131 ----YNSIAIIDADGTDLGIYRKSHI 152
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 39.5 bits (88), Expect = 0.002
Identities = 20/54 (37%), Positives = 29/54 (53%)
Frame = +3
Query: 270 FNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQNGAEIVFNPSA 431
+ E Y+ G+TG VF T++ KI V IC+ + GAEI+F P+A
Sbjct: 131 YQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTA 184
Score = 36.3 bits (80), Expect = 0.020
Identities = 25/86 (29%), Positives = 47/86 (54%), Gaps = 1/86 (1%)
Frame = +1
Query: 1 ICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVI-VSSILERDEKH 177
I QEL+ + FC +++ + + A+ ++ PT +++LA + +VI VS E + H
Sbjct: 45 ILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVIPVSFFEEANTAH 103
Query: 178 SDILWNTAVVISDTGNVIGKHRKNHI 255
+N+ +I G +G +RK+HI
Sbjct: 104 ----YNSIAIIDADGTDLGIYRKSHI 125
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 32.7 bits (71), Expect = 0.25
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = +1
Query: 91 GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 246
GP L ELA K + +V +E+D L+ TA+ S G +GKHRK
Sbjct: 97 GPEVEKLAELAGKNNVYLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRK 145
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 31.9 bits (69), Expect = 0.43
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = +1
Query: 91 GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 246
GP L ELA K + +V +E+D L+ TA+ S G +GKHRK
Sbjct: 104 GPEVERLAELAGKNNVHLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRK 152
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 30.3 bits (65), Expect = 1.3
Identities = 17/52 (32%), Positives = 26/52 (50%)
Frame = +1
Query: 91 GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 246
GP L +A KY + +V ++ER+ L+ T + G +GKHRK
Sbjct: 115 GPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHRK 163
>At5g67240.1 68418.m08475 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 745
Score = 29.1 bits (62), Expect = 3.0
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 99 HDLPSGTRHQVRNGDRVLDIRKGREAFGH 185
HD+PS H V NGD LD++ + G+
Sbjct: 441 HDVPSQELHGVLNGDFTLDVKPPKRKGGY 469
>At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family protein
similar to Anther-specific proline-rich protein APG from
Brassica napus (SP|P40603 ), Arabidopsis thaliana
(GI:22599); contains Pfam profile PF00657:
Lipase/Acylhydrolase with GDSL-like motif
Length = 367
Score = 29.1 bits (62), Expect = 3.0
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
Frame = -1
Query: 502 QFVIAAFLASTFHMYSLP---PSPAIVADGLKTISAPFCPNIIQ--FKTWCRP 359
+F+ +FL S ++ Y L PS V L +++P CPN+ T C+P
Sbjct: 271 KFIKTSFLYSDYYNYMLGLRGPSSNQVGSSLLNVTSPCCPNVYDGGQLTSCKP 323
>At5g06970.1 68418.m00789 expressed protein
Length = 1101
Score = 28.7 bits (61), Expect = 4.0
Identities = 13/59 (22%), Positives = 27/59 (45%)
Frame = -3
Query: 242 RCFPITFPVSLITTAVFQSMSECFSSLSNIEDTITIAYLMASSRRKVVVGPSSADSANS 66
R I P + + ++ S LS +ED++ + ++ R K+V+ S + + S
Sbjct: 834 RSINIDVPATAMLCVQLNTLHYAVSQLSKLEDSMWLRWIAKKPREKIVIRKSMVEKSKS 892
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 28.3 bits (60), Expect = 5.3
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +1
Query: 91 GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 246
GP L ++A K + +V +E K L+ T + S G +GKHRK
Sbjct: 104 GPEVARLADVARKNHVYLVMGAIE---KEGYTLYCTVLFFSPQGQFLGKHRK 152
>At2g44760.1 68415.m05571 expressed protein
Length = 504
Score = 27.9 bits (59), Expect = 7.0
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +3
Query: 261 VGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQNGAEI 413
VG+ +ESNYY+ P F ++ + V C R V++ + F GA+I
Sbjct: 291 VGEMSESNYYLT-----PTF--KFEQCLVKGCHKRLRVVHTIEFANGGADI 334
>At2g39980.1 68415.m04913 transferase family protein contains Pfam
profile PF02458 transferase family
Length = 482
Score = 27.9 bits (59), Expect = 7.0
Identities = 12/28 (42%), Positives = 14/28 (50%)
Frame = -1
Query: 217 CH*LQPQCSKVCPNASRPFLISRTRSPL 134
CH +Q C CPN P LIS + L
Sbjct: 39 CHYIQKGCLFTCPNLPLPALISHLKHSL 66
>At3g48580.1 68416.m05304 xyloglucan:xyloglucosyl transferase,
putative / xyloglucan endotransglycosylase, putative /
endo-xyloglucan transferase, putative similar to
endoxyloglucan transferase EXGT-A4 GI:5139002 from
[Arabidopsis thaliana]
Length = 277
Score = 27.5 bits (58), Expect = 9.2
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 363 RHHVLNWMMFGQNGAEIVFNPSATIAGEGGSE 458
RH L + + G+NG + N + + GEGG +
Sbjct: 104 RHDELCFQILGKNGPPYLLNTNMYLYGEGGKD 135
>At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N
domain-containing protein contains Pfam profile PF00627:
UBA/TS-N domain
Length = 649
Score = 27.5 bits (58), Expect = 9.2
Identities = 17/38 (44%), Positives = 25/38 (65%), Gaps = 2/38 (5%)
Frame = -3
Query: 218 VSLITTAVFQSMSECFSSLSNIEDT-ITIAYL-MASSR 111
+ L + + FQ+ FSSL+NI+ T T+A L MASS+
Sbjct: 482 IGLPSQSQFQASGSSFSSLTNIDPTDFTVANLPMASSQ 519
>At1g10190.1 68414.m01149 expressed protein similar to hypothetical
protein GB:CAB10284 contains Pfam profile PF03080:
Arabidopsis proteins of unknown function
Length = 396
Score = 27.5 bits (58), Expect = 9.2
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +3
Query: 381 WMMFGQNGAEIVFNPSATIAGEGGSEYMWNVEARNAA 491
W+ FG E+ F PS+ G+ W EA +A+
Sbjct: 283 WLQFGNAAEEVGFWPSSRFHQSSGNLVEWGGEAYSAS 319
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,361,898
Number of Sequences: 28952
Number of extensions: 326389
Number of successful extensions: 939
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 896
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 935
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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