Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0614
(729 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g34100.1 68414.m04229 choline kinase, putative similar to cho... 64 1e-10
At1g74320.1 68414.m08606 choline kinase, putative similar to GmC... 59 3e-09
At4g09760.2 68417.m01603 choline kinase, putative similar to GmC... 58 5e-09
At4g09760.1 68417.m01602 choline kinase, putative similar to GmC... 58 5e-09
At1g71697.1 68414.m08288 choline kinase, putative similar to GmC... 57 1e-08
At2g26830.1 68415.m03219 choline/ethanolamine kinase family prot... 56 2e-08
At2g41050.1 68415.m05069 PQ-loop repeat family protein / transme... 32 0.34
At1g22720.1 68414.m02839 wall-associated kinase, putative contai... 32 0.34
At3g45710.1 68416.m04940 proton-dependent oligopeptide transport... 32 0.45
At3g11130.1 68416.m01349 clathrin heavy chain, putative similar ... 31 0.59
At5g15110.1 68418.m01770 pectate lyase family protein similar to... 29 3.2
At5g01950.1 68418.m00114 leucine-rich repeat transmembrane prote... 29 3.2
At3g45700.1 68416.m04939 proton-dependent oligopeptide transport... 29 3.2
At3g45680.1 68416.m04937 proton-dependent oligopeptide transport... 29 4.2
At5g35695.1 68418.m04268 hypothetical protein 28 5.5
At3g53590.1 68416.m05919 leucine-rich repeat transmembrane prote... 28 5.5
At2g05860.1 68415.m00635 hypothetical protein similar to At2g108... 28 5.5
At5g35790.1 68418.m04292 glucose-6-phosphate 1-dehydrogenase / G... 27 9.6
At2g03000.1 68415.m00252 zinc finger (C3HC4-type RING finger) fa... 27 9.6
At1g06840.1 68414.m00729 leucine-rich repeat transmembrane prote... 27 9.6
>At1g34100.1 68414.m04229 choline kinase, putative similar to
choline kinase (GmCK2p) GI:1438881 from [Glycine max];
contains Pfam profile PF01633: Choline/ethanolamine
kinase; could not find a suitable start codon; this may
be a pseudogene, a transposon insertion is found
immediately upstream.
Length = 267
Score = 63.7 bits (148), Expect = 1e-10
Identities = 27/62 (43%), Positives = 39/62 (62%)
Frame = +2
Query: 263 QEPSIDDVNHLLNEVEAFALASHLFWTLWSIVNASKSQIPFGYWEYALSRFETYQRLKIE 442
+EP DD+ LL + E + LASHLFW LW I++ ++I F Y EY+ RF+ Y K E
Sbjct: 195 EEPKEDDIKQLLKDAEKYTLASHLFWGLWGIISGYVNKIDFDYAEYSRQRFKQYWLRKPE 254
Query: 443 VI 448
++
Sbjct: 255 LL 256
Score = 40.3 bits (90), Expect = 0.001
Identities = 26/71 (36%), Positives = 33/71 (46%), Gaps = 4/71 (5%)
Frame = +3
Query: 63 LIDFEYCAYNHRGFDIANHFQEWAYDY----TNDAHPFYHECRDDCPTLEQKEIFIKEYL 230
+ID+EY +YN +DIANHF E A +Y + F H E KE IK+ L
Sbjct: 148 IIDYEYASYNPVAYDIANHFCEMAANYKVAGEEERRRFIHNYLSSSGE-EPKEDDIKQLL 206
Query: 231 KHYSSNVSRSH 263
K SH
Sbjct: 207 KDAEKYTLASH 217
>At1g74320.1 68414.m08606 choline kinase, putative similar to GmCK2p
choline kinase gi|1438881|gb|AAC49375
Length = 350
Score = 58.8 bits (136), Expect = 3e-09
Identities = 26/54 (48%), Positives = 34/54 (62%)
Frame = +2
Query: 263 QEPSIDDVNHLLNEVEAFALASHLFWTLWSIVNASKSQIPFGYWEYALSRFETY 424
++PS V LL +VE + LASHL W LW I++ ++I F Y EYA RFE Y
Sbjct: 283 EKPSDTMVKKLLEDVEKYTLASHLIWGLWGIISEHVNEIDFDYMEYARQRFEQY 336
Score = 45.2 bits (102), Expect = 4e-05
Identities = 22/58 (37%), Positives = 34/58 (58%)
Frame = +3
Query: 63 LIDFEYCAYNHRGFDIANHFQEWAYDYTNDAHPFYHECRDDCPTLEQKEIFIKEYLKH 236
+ID+EY YN +DIANHF E A DY + P + P +E+++ F+K Y+ +
Sbjct: 225 IIDYEYSCYNPVAYDIANHFCEMAADYHTET-PHIMD-YSKYPGVEERQRFLKTYMSY 280
>At4g09760.2 68417.m01603 choline kinase, putative similar to GmCK2p
choline kinase gi|1438881|gb|AAC49375
Length = 346
Score = 58.4 bits (135), Expect = 5e-09
Identities = 23/54 (42%), Positives = 36/54 (66%)
Frame = +2
Query: 263 QEPSIDDVNHLLNEVEAFALASHLFWTLWSIVNASKSQIPFGYWEYALSRFETY 424
+E +D+ LL+++E + LASHLFW LW I++ ++I F Y EY+ RF+ Y
Sbjct: 280 EEAREEDIEQLLDDIEKYTLASHLFWGLWGIISGYVNKIEFDYIEYSRQRFKQY 333
Score = 41.9 bits (94), Expect = 4e-04
Identities = 24/57 (42%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
Frame = +3
Query: 63 LIDFEYCAYNHRGFDIANHFQEWAYDY-TNDAHPFYHECRDDCPTLEQKEIFIKEYL 230
+ID+EY +YN +DIANHF E A DY +N H + P E++ FI YL
Sbjct: 222 IIDYEYASYNPIAYDIANHFCEMAADYHSNTPHILDYTL---YPGEEERRRFICNYL 275
>At4g09760.1 68417.m01602 choline kinase, putative similar to GmCK2p
choline kinase gi|1438881|gb|AAC49375
Length = 346
Score = 58.4 bits (135), Expect = 5e-09
Identities = 23/54 (42%), Positives = 36/54 (66%)
Frame = +2
Query: 263 QEPSIDDVNHLLNEVEAFALASHLFWTLWSIVNASKSQIPFGYWEYALSRFETY 424
+E +D+ LL+++E + LASHLFW LW I++ ++I F Y EY+ RF+ Y
Sbjct: 280 EEAREEDIEQLLDDIEKYTLASHLFWGLWGIISGYVNKIEFDYIEYSRQRFKQY 333
Score = 41.9 bits (94), Expect = 4e-04
Identities = 24/57 (42%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
Frame = +3
Query: 63 LIDFEYCAYNHRGFDIANHFQEWAYDY-TNDAHPFYHECRDDCPTLEQKEIFIKEYL 230
+ID+EY +YN +DIANHF E A DY +N H + P E++ FI YL
Sbjct: 222 IIDYEYASYNPIAYDIANHFCEMAADYHSNTPHILDYTL---YPGEEERRRFICNYL 275
>At1g71697.1 68414.m08288 choline kinase, putative similar to GmCK2p
choline kinase gi|1438881|gb|AAC49375
Length = 346
Score = 56.8 bits (131), Expect = 1e-08
Identities = 22/48 (45%), Positives = 32/48 (66%)
Frame = +2
Query: 281 DVNHLLNEVEAFALASHLFWTLWSIVNASKSQIPFGYWEYALSRFETY 424
+V LL + E++ LA+H+FW LW I++ ++I F Y EYA RFE Y
Sbjct: 289 EVERLLKDAESYTLANHIFWGLWGIISGHVNKIEFDYMEYARQRFEQY 336
Score = 37.9 bits (84), Expect = 0.007
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = +3
Query: 63 LIDFEYCAYNHRGFDIANHFQEWAYDYTND 152
+ID+EY ++N +DIANHF E A +Y +D
Sbjct: 225 IIDYEYSSFNPIAYDIANHFCEMAANYHSD 254
>At2g26830.1 68415.m03219 choline/ethanolamine kinase family protein
similar to SP|Q9HBU6 Ethanolamine kinase (EC 2.7.1.82)
(EKI){Homo sapiens}, GmCK3p [Glycine max] GI:1438883;
contains Pfam profile PF01633: Choline/ethanolamine
kinase
Length = 374
Score = 56.4 bits (130), Expect = 2e-08
Identities = 24/60 (40%), Positives = 36/60 (60%)
Frame = +2
Query: 257 EPQEPSIDDVNHLLNEVEAFALASHLFWTLWSIVNASKSQIPFGYWEYALSRFETYQRLK 436
+P E SI +V + E +A+ LASHL+W +W+I+ A S I F Y Y R+ Y++ K
Sbjct: 300 KPDEVSIAEVESVFVETDAYKLASHLYWAIWAIIQARMSPIEFEYLGYFFLRYNEYKKQK 359
Score = 54.8 bits (126), Expect = 6e-08
Identities = 31/62 (50%), Positives = 40/62 (64%)
Frame = +3
Query: 48 EPKLFLIDFEYCAYNHRGFDIANHFQEWAYDYTNDAHPFYHECRDDCPTLEQKEIFIKEY 227
E KL+LIDFEY +YN+RGFDI NHF E+A Y D + Y P+ E++ FIK Y
Sbjct: 245 EEKLYLIDFEYGSYNYRGFDIGNHFNEYA-GYDCD-YSLY-------PSKEEQYHFIKHY 295
Query: 228 LK 233
L+
Sbjct: 296 LQ 297
>At2g41050.1 68415.m05069 PQ-loop repeat family protein /
transmembrane family protein similar to SP|Q10482 Seven
transmembrane protein 1 {Schizosaccharomyces pombe};
contains Pfam profile PF04193: PQ loop repeat
Length = 376
Score = 32.3 bits (70), Expect = 0.34
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +3
Query: 303 RWKRSPSPATCSGRSGPLSMLPRVRYPSDTG 395
RW+ S TC G++ P++M+P + S TG
Sbjct: 125 RWRNSSDTTTCGGQTTPITMIPGSQRTSFTG 155
>At1g22720.1 68414.m02839 wall-associated kinase, putative contains
similarity to serine/threonine kinase gb|Y12531 from
Brassica oleracea
Length = 219
Score = 32.3 bits (70), Expect = 0.34
Identities = 13/51 (25%), Positives = 27/51 (52%)
Frame = +2
Query: 182 RLPDLGAKGNIHKGVLKTLFVERLQEPQEPSIDDVNHLLNEVEAFALASHL 334
R+ G +G ++KG+L F+ +++ + V H +NEV + +H+
Sbjct: 140 RILGQGGQGKVYKGILSDNFIVAIKKARLGDSSQVEHFINEVLILSQINHM 190
>At3g45710.1 68416.m04940 proton-dependent oligopeptide transport
(POT) family protein contains Pfam profile: PF00854 POT
family
Length = 560
Score = 31.9 bits (69), Expect = 0.45
Identities = 25/100 (25%), Positives = 45/100 (45%), Gaps = 3/100 (3%)
Frame = -1
Query: 666 FNRHITISLCNSAHSLKNTAT*LGMVTSVRTFYVTTPSIDLIRQLHG--PSQLGYSHMST 493
F ++ + SLKNTAT L V +FY++T ID+I++ P+ + + +
Sbjct: 459 FPANVAVFYGEFPESLKNTATSLTSVVIGISFYLSTAVIDVIQRTTSWLPNDINHGRVDN 518
Query: 492 SH-IFRFWGRPQLSFFMTSILRRWYVSNLDSAYSQYPKGI 376
+ + G L +F+ Y + D + Q PK +
Sbjct: 519 VYWVVVIGGVLNLGYFLVCSWFYKYRNLKDDDHEQDPKDV 558
>At3g11130.1 68416.m01349 clathrin heavy chain, putative similar to
Swiss-Prot:Q00610 clathrin heavy chain 1 (CLH-17) [Homo
sapiens]
Length = 1705
Score = 31.5 bits (68), Expect = 0.59
Identities = 18/85 (21%), Positives = 37/85 (43%)
Frame = -3
Query: 526 PQSVGILSYVDLAYFSFLGQTAVVLLYDLNLETLVRFEPRQRVFPVSEGYLTLGSIDNGP 347
P+ +G+++ + ++S G + V ++D + P + + +G P
Sbjct: 122 PKMLGLVTQTSVYHWSIEGDSEPVKMFDRTANLANNQIINYKCSPNEKWLVLIGIAPGSP 181
Query: 346 ERPEQVAGEGERFHLVQQMINVIYA 272
ERP+ V G + F + QQ + A
Sbjct: 182 ERPQLVKGNMQLFSVDQQRSQALEA 206
>At5g15110.1 68418.m01770 pectate lyase family protein similar to
pectate lyase P59 SP:P15722 from [Lycopersicon
esculentum]
Length = 472
Score = 29.1 bits (62), Expect = 3.2
Identities = 23/68 (33%), Positives = 34/68 (50%)
Frame = +3
Query: 69 DFEYCAYNHRGFDIANHFQEWAYDYTNDAHPFYHECRDDCPTLEQKEIFIKEYLKHYSSN 248
+F AY+ ++I +HF E YD + D E D P E+KE FI E L +++
Sbjct: 42 EFTLQAYHSDPYEIVDHFHERHYDNSTDV-TTPEEDGDAKPEEEEKE-FI-EMLGSSTNS 98
Query: 249 VSRSHRSR 272
RS R +
Sbjct: 99 TRRSLRGK 106
>At5g01950.1 68418.m00114 leucine-rich repeat transmembrane protein
kinase, putative receptor protein kinases
Length = 1032
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/45 (28%), Positives = 21/45 (46%)
Frame = +2
Query: 197 GAKGNIHKGVLKTLFVERLQEPQEPSIDDVNHLLNEVEAFALASH 331
G G +++GVL V ++ E S+ LNE+E + H
Sbjct: 716 GGYGKVYRGVLSDNTVAAIKRADEGSLQGEKEFLNEIELLSRLHH 760
>At3g45700.1 68416.m04939 proton-dependent oligopeptide transport
(POT) family protein contains Pfam profile: PF00854 POT
family
Length = 548
Score = 29.1 bits (62), Expect = 3.2
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = -1
Query: 666 FNRHITISLCNSAHSLKNTAT*LGMVTSVRTFYVTTPSIDLIRQ 535
F +I I SL+NTAT L V +FY++T ID+I++
Sbjct: 447 FPANIAIFYGEFPESLRNTATSLTSVVMGISFYLSTALIDVIQR 490
>At3g45680.1 68416.m04937 proton-dependent oligopeptide transport
(POT) family protein contains Pfam profile: PF00854 POT
family
Length = 558
Score = 28.7 bits (61), Expect = 4.2
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = -1
Query: 624 SLKNTAT*LGMVTSVRTFYVTTPSIDLIRQ 535
SL+NTAT L V +FY++T IDLI++
Sbjct: 471 SLRNTATSLTSVVIGISFYLSTALIDLIQR 500
>At5g35695.1 68418.m04268 hypothetical protein
Length = 211
Score = 28.3 bits (60), Expect = 5.5
Identities = 14/25 (56%), Positives = 14/25 (56%)
Frame = +2
Query: 11 VRQHLQPHLGQRRAEALPHRLRVLR 85
VR HLQ GQRR PH L LR
Sbjct: 69 VRYHLQEFAGQRRDPETPHELFNLR 93
>At3g53590.1 68416.m05919 leucine-rich repeat transmembrane protein
kinase, putative CLV1 receptor kinase, Arabidopsis
thaliana, EMBL:ATU96879
Length = 783
Score = 28.3 bits (60), Expect = 5.5
Identities = 11/45 (24%), Positives = 22/45 (48%)
Frame = +2
Query: 197 GAKGNIHKGVLKTLFVERLQEPQEPSIDDVNHLLNEVEAFALASH 331
G+ G ++KG+L ++ +E S+ LNE++ + H
Sbjct: 444 GSYGKVYKGILSNKTEVAIKRGEETSLQSEKEFLNEIDLLSRLHH 488
>At2g05860.1 68415.m00635 hypothetical protein similar to At2g10840,
At5g35280, At2g10500, At4g07310, At4g07430, At1g39270
Length = 238
Score = 28.3 bits (60), Expect = 5.5
Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 1/68 (1%)
Frame = +1
Query: 454 GQLRSAPETKNMRGRHMRVSQLTGAVQLSD*IDR-WRCYIKSSD*CHHTQLGGGVFKRMG 630
G + E N R + V LT ++SD +R +C + ++ + GGGV +
Sbjct: 138 GAIYDVGELINTRPKQNNVDDLTLTFKISDNENRVLKCLATKKEAHNYRRYGGGVIVVVL 197
Query: 631 GVAKTDRY 654
G K DRY
Sbjct: 198 GWWKIDRY 205
>At5g35790.1 68418.m04292 glucose-6-phosphate 1-dehydrogenase / G6PD
(APG1) identical to SP|Q43727 Glucose-6-phosphate
1-dehydrogenase, cytoplasmic isoform (EC 1.1.1.49)
(G6PD) {Arabidopsis thaliana}
Length = 576
Score = 27.5 bits (58), Expect = 9.6
Identities = 17/44 (38%), Positives = 27/44 (61%), Gaps = 5/44 (11%)
Frame = +2
Query: 77 VLRLQP-QG--LRHSEPLPGMGLRLHERRASVL--PRVPGRLPD 193
V+R+QP +G LR + +PG+G+RL ++L R P +PD
Sbjct: 459 VIRVQPDEGIYLRINNKVPGLGMRLDRSDLNLLYRSRYPREIPD 502
>At2g03000.1 68415.m00252 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 535
Score = 27.5 bits (58), Expect = 9.6
Identities = 23/75 (30%), Positives = 36/75 (48%), Gaps = 4/75 (5%)
Frame = +3
Query: 306 WKRSPSPATCSGRSGPLSMLPRVRYPSDT--GNTRCRGSKRTNVSRLRS*RRT--TAVCP 473
W+ + +P+T S + P S PRV S + G + S R NV S R T ++
Sbjct: 181 WETNTTPSTRSWPTVPSSNSPRVLQTSMSRRGTSPMSSSTRRNVQSSMSGRETVSSSTST 240
Query: 474 RNEKYARSTYESIPT 518
+ + + ST E +PT
Sbjct: 241 SSMQTSMSTPEIMPT 255
>At1g06840.1 68414.m00729 leucine-rich repeat transmembrane protein
kinase, putative similar to receptor protein kinase
GB:BAA11869 GI:1389566 from [Arabidopsis thaliana]
Length = 939
Score = 27.5 bits (58), Expect = 9.6
Identities = 13/45 (28%), Positives = 21/45 (46%)
Frame = +2
Query: 197 GAKGNIHKGVLKTLFVERLQEPQEPSIDDVNHLLNEVEAFALASH 331
G G ++KG L + V ++ QE S+ L E+E + H
Sbjct: 620 GGYGKVYKGTLGSGTVVAIKRAQEGSLQGEKEFLTEIELLSRLHH 664
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,583,760
Number of Sequences: 28952
Number of extensions: 346988
Number of successful extensions: 1104
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 966
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1104
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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