Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0609
(740 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 104 1e-23
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 88 1e-18
SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 74 2e-14
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 68 2e-12
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 64 3e-11
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 50 3e-07
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 28 1.2
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 2.8
SPBC354.08c |||DUF221 family protein|Schizosaccharomyces pombe|c... 25 8.6
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 104 bits (250), Expect = 1e-23
Identities = 46/85 (54%), Positives = 63/85 (74%), Gaps = 1/85 (1%)
Frame = -2
Query: 508 DVRKQLLGIMDRHKLDVVSAGKNTVRIQKTICSGFFRNAAKKDPQEG-YRTLVDSQVVYI 332
DVRKQL+ +MDR++ VVS G+ I + +CSG+F N AK+D EG Y+T+V++ VY+
Sbjct: 1023 DVRKQLIRLMDRYRHPVVSCGRKRELILRALCSGYFTNVAKRDSHEGCYKTIVENAPVYM 1082
Query: 331 HPSSALFNRQPEWVIYHELVQTTKE 257
HPS LF + EWVIYHEL+QT+KE
Sbjct: 1083 HPSGVLFGKAAEWVIYHELIQTSKE 1107
Score = 82.6 bits (195), Expect = 5e-17
Identities = 34/76 (44%), Positives = 52/76 (68%)
Frame = -3
Query: 735 CSDEILTIVSMLSVQNVFYRPXXXXXXXXXXXXKFNQPEGDHLTLLAVYNSWRNNKFSNA 556
CS+E+L+I++MLSV N++ RP +F PE DHLTLL VY +W+ N+ S+
Sbjct: 947 CSEEMLSIIAMLSVPNIWSRPREKQQEADRQRAQFANPESDHLTLLNVYTTWKMNRCSDN 1006
Query: 555 WCYENFVQIRTLKRAQ 508
WCYE+++Q R ++RA+
Sbjct: 1007 WCYEHYIQARGMRRAE 1022
Score = 70.1 bits (164), Expect = 3e-13
Identities = 27/50 (54%), Positives = 40/50 (80%)
Frame = -1
Query: 254 MREVTTIDPKWLVEFAPAFFKFSDPTKLSKFKKNQRLEPLYNKYEEPNAW 105
M V+T++PKWLVE AP FFKF++ ++SK KKN ++ PLYN++E+P+ W
Sbjct: 1109 MHTVSTVNPKWLVEVAPTFFKFANANQVSKTKKNLKVLPLYNRFEKPDEW 1158
>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
Prp16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 88.2 bits (209), Expect = 1e-18
Identities = 39/77 (50%), Positives = 53/77 (68%)
Frame = -3
Query: 738 QCSDEILTIVSMLSVQNVFYRPXXXXXXXXXXXXKFNQPEGDHLTLLAVYNSWRNNKFSN 559
+C++EI+TIVSMLSV +VFYRP KFN PE DHL LL +Y W+ N +SN
Sbjct: 926 KCTEEIITIVSMLSVPSVFYRPKERAEESDAAREKFNVPESDHLMLLNIYQHWQRNGYSN 985
Query: 558 AWCYENFVQIRTLKRAQ 508
+WC ++F+ +TLKRA+
Sbjct: 986 SWCSKHFLHSKTLKRAR 1002
Score = 52.8 bits (121), Expect = 5e-08
Identities = 29/86 (33%), Positives = 49/86 (56%), Gaps = 2/86 (2%)
Frame = -2
Query: 508 DVRKQLLGIMDRHKLDVVSAGKNTVRIQKTICSGFFRNAAKKDPQEGYRTLVDSQVVYIH 329
D+R+QL+ IM + K+ + S + +++ +CS +F AA Y L ++H
Sbjct: 1003 DIRQQLVEIMSKQKISLESVSDWDI-VRRVLCSAYFHQAACAKGIGEYVHLRSGMPCHLH 1061
Query: 328 PSSALFNRQ--PEWVIYHELVQTTKE 257
+S+L+ P++VIYHELV T+KE
Sbjct: 1062 VTSSLYGLGYLPDYVIYHELVLTSKE 1087
Score = 27.1 bits (57), Expect = 2.8
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = -1
Query: 254 MREVTTIDPKWLVEFAPAFFKFSD 183
M VT++DP WL EF ++ +
Sbjct: 1089 MNIVTSVDPYWLAEFGGVYYSVKE 1112
>SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1055
Score = 74.1 bits (174), Expect = 2e-14
Identities = 37/86 (43%), Positives = 59/86 (68%), Gaps = 2/86 (2%)
Frame = -2
Query: 508 DVRKQLLGIMDRHKLDVVSAGKNTVR-IQKTICSGFFRNAAKKDPQ-EGYRTLVDSQVVY 335
DVR QL + +R ++++V+ ++ I+K I +G+F NAA+ D + YRT+ +Q VY
Sbjct: 932 DVRDQLANLCERVEIELVTNSSESLDPIKKAITAGYFSNAARLDRSGDSYRTVKSNQTVY 991
Query: 334 IHPSSALFNRQPEWVIYHELVQTTKE 257
IHPSS++ ++P+ +IY ELV TTKE
Sbjct: 992 IHPSSSVAEKKPKVIIYFELVLTTKE 1017
Score = 71.3 bits (167), Expect = 1e-13
Identities = 34/77 (44%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
Frame = -3
Query: 735 CSDEILTIVSMLS-VQNVFYRPXXXXXXXXXXXXKFNQPEGDHLTLLAVYNSWRNNKFSN 559
C +E+L+IVSML ++FYRP F QP GDHLTLL ++N W + FS
Sbjct: 855 CVEEVLSIVSMLGEASSLFYRPKDKIMEADKARANFTQPGGDHLTLLHIWNEWVDTDFSY 914
Query: 558 AWCYENFVQIRTLKRAQ 508
W ENF+Q ++L RA+
Sbjct: 915 NWARENFLQYKSLCRAR 931
Score = 34.7 bits (76), Expect = 0.014
Identities = 13/35 (37%), Positives = 23/35 (65%)
Frame = -1
Query: 251 REVTTIDPKWLVEFAPAFFKFSDPTKLSKFKKNQR 147
R++T I P+WL+E +P +FK + +L K +K +
Sbjct: 1020 RQITEIQPEWLLEISPHYFKPENIEELQKTQKRHK 1054
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 67.7 bits (158), Expect = 2e-12
Identities = 39/88 (44%), Positives = 56/88 (63%), Gaps = 5/88 (5%)
Frame = -2
Query: 508 DVRKQLLGIMDRHKLDVVSA---GKNT-VRIQKTICSGFFRNAAKKDPQ-EGYRTLVDSQ 344
+VRKQL M+R +++++S KN V I++ + SGFF AKK + Y T+ D+Q
Sbjct: 596 NVRKQLRRTMERQEVELISTPFDDKNYYVNIRRALVSGFFMQVAKKSANGKNYVTMKDNQ 655
Query: 343 VVYIHPSSALFNRQPEWVIYHELVQTTK 260
VV +HPS L + PEWV+Y+E V TTK
Sbjct: 656 VVSLHPSCGL-SVTPEWVVYNEFVLTTK 682
Score = 66.9 bits (156), Expect = 3e-12
Identities = 28/72 (38%), Positives = 46/72 (63%)
Frame = -3
Query: 735 CSDEILTIVSMLSVQNVFYRPXXXXXXXXXXXXKFNQPEGDHLTLLAVYNSWRNNKFSNA 556
CS+E+L++ ++LSV NVF RP +F P+GDHLTLL VY+++++ + +
Sbjct: 520 CSNEVLSLTALLSVPNVFVRPNSARKLADEMRQQFTHPDGDHLTLLNVYHAYKSGEGTAD 579
Query: 555 WCYENFVQIRTL 520
WC+ +F+ R L
Sbjct: 580 WCWNHFLSHRAL 591
Score = 31.5 bits (68), Expect = 0.13
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = -1
Query: 254 MREVTTIDPKWLVEFAPAFFKFSD 183
+R VT I P+WL+E AP ++ D
Sbjct: 685 IRNVTAIRPEWLIELAPNYYDLDD 708
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 63.7 bits (148), Expect = 3e-11
Identities = 37/109 (33%), Positives = 61/109 (55%), Gaps = 1/109 (0%)
Frame = -2
Query: 508 DVRKQLLGIMDRHKLDVVSAGK-NTVRIQKTICSGFFRNAAKKDPQEGYRTLVDSQVVYI 332
D+RKQL + ++ S+ + N+ + + SG+ N A P YRT++ +Q + I
Sbjct: 609 DIRKQLREHCLKDGWELNSSPEVNSENLLLSFLSGYITNTALLHPDGSYRTIIGNQTISI 668
Query: 331 HPSSALFNRQPEWVIYHELVQTTKE**GKSRP*TRNGWLSLLPPSLNSR 185
HPSS+LF ++ E ++YHELV TTK + R+ WL+ + P +R
Sbjct: 669 HPSSSLFGKKVEAIMYHELVFTTKS-YVRGVSSIRSNWLNAVAPHYLAR 716
Score = 40.3 bits (90), Expect = 3e-04
Identities = 23/75 (30%), Positives = 33/75 (44%), Gaps = 2/75 (2%)
Frame = -3
Query: 735 CSDEILTIVSMLSVQNVFYRPXXXXXXXXXXXXKFNQPEGDHLTLLAVYNSWRNNKFSNA 556
C E++ +VS LS ++F P KF EGD LT L + + +
Sbjct: 531 CLSEVIDVVSCLSTDSMFLFPQEKRDEAIEARLKFLHSEGDLLTCLNALRQYLESSHDSR 590
Query: 555 --WCYENFVQIRTLK 517
WC +NF+ R LK
Sbjct: 591 KQWCSQNFINRRALK 605
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 50.4 bits (115), Expect = 3e-07
Identities = 27/76 (35%), Positives = 40/76 (52%), Gaps = 1/76 (1%)
Frame = -3
Query: 735 CSDEILTIVSMLSVQNVFYRPXXXXXXXXXXXXK-FNQPEGDHLTLLAVYNSWRNNKFSN 559
C+ EIL+I S+L+ VFY P F EGD +T L V+ S+ NK
Sbjct: 464 CTHEILSIASILTAGEVFYNPTSSSKNDAFVAHSSFFANEGDIITALNVFESFVGNKKDL 523
Query: 558 AWCYENFVQIRTLKRA 511
WC +N++ +TL++A
Sbjct: 524 QWCRKNYLNYQTLRQA 539
Score = 38.3 bits (85), Expect = 0.001
Identities = 21/90 (23%), Positives = 41/90 (45%), Gaps = 2/90 (2%)
Frame = -2
Query: 529 QDVEAGPDVRKQLLGIMDRHKLDVVSA--GKNTVRIQKTICSGFFRNAAKKDPQEGYRTL 356
Q + D+R L+ +++ + + +I K + GF RN A Y+T+
Sbjct: 534 QTLRQALDIRTHLVRFLNKFSIPTAQRLPSSDCSKILKCLLDGFVRNVAHLQNDGSYKTI 593
Query: 355 VDSQVVYIHPSSALFNRQPEWVIYHELVQT 266
Q V++ SS L ++ W++Y V++
Sbjct: 594 GGKQ-VWLDSSSVLHEKKTPWIMYSSAVES 622
>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1327
Score = 28.3 bits (60), Expect = 1.2
Identities = 14/36 (38%), Positives = 22/36 (61%), Gaps = 5/36 (13%)
Frame = -2
Query: 352 DSQVVYIHPSSALFNRQPE-----WVIYHELVQTTK 260
++Q ++IHPSS +F P +V Y + V+TTK
Sbjct: 1203 NNQRLFIHPSSTMFVNSPNASRCTFVAYEQKVETTK 1238
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 27.1 bits (57), Expect = 2.8
Identities = 13/65 (20%), Positives = 30/65 (46%)
Frame = -2
Query: 475 RHKLDVVSAGKNTVRIQKTICSGFFRNAAKKDPQEGYRTLVDSQVVYIHPSSALFNRQPE 296
+H + + T+R Q++ CS F + +KD T+VD + + + + +
Sbjct: 33 KHNFSITNKSLETLRSQESPCSTTFPVSDRKDALLNIPTVVDDEFQHTEELAGVSSWSDF 92
Query: 295 WVIYH 281
+ ++H
Sbjct: 93 FYLFH 97
>SPBC354.08c |||DUF221 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 865
Score = 25.4 bits (53), Expect = 8.6
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +3
Query: 231 VYGRDFPHYSFVVCTSSW*ITHSGCLLNSALEG 329
+Y +F HY F+ SS+ I H NS + G
Sbjct: 311 LYENEFEHYGFITYKSSY-IAHDTARHNSRVAG 342
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,841,768
Number of Sequences: 5004
Number of extensions: 56759
Number of successful extensions: 149
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -