BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0603 (700 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g66150.1 68414.m07508 leucine-rich repeat protein kinase, put... 30 1.7 At5g53440.1 68418.m06641 expressed protein 29 2.2 At4g29000.1 68417.m04145 tesmin/TSO1-like CXC domain-containing ... 29 3.0 At2g34080.1 68415.m04172 cysteine proteinase, putative contains ... 29 3.9 At1g02280.1 68414.m00169 GTP-binding protein (TOC33) identical t... 28 5.2 At5g16160.1 68418.m01889 expressed protein 28 6.8 At4g02650.1 68417.m00360 epsin N-terminal homology (ENTH) domain... 27 9.0 >At1g66150.1 68414.m07508 leucine-rich repeat protein kinase, putative (TMK1) identical to protein kinase TMK1 gi|166888|gb|AAA32876, SP|P43298 Putative receptor protein kinase TMK1 precursor (EC 2.7.1.-) {Arabidopsis thaliana} Length = 942 Score = 29.9 bits (64), Expect = 1.7 Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 2/50 (4%) Frame = -1 Query: 649 PVSCRDISEFKNFGWTSINLLGSMFEFVGPS--AGILLCSIALDIISSDL 506 P S R+ S +NF S N+ GS+ F+GP G+ + +A + + +L Sbjct: 153 PESLRNASALQNFSANSANVSGSLPGFLGPDEFPGLSILHLAFNNLEGEL 202 >At5g53440.1 68418.m06641 expressed protein Length = 1181 Score = 29.5 bits (63), Expect = 2.2 Identities = 14/35 (40%), Positives = 21/35 (60%) Frame = +3 Query: 312 RRKLRKLEQLTASKSKKKSKRIGNSESYIPENNLE 416 + LR E+LT +SKKKSK N + P++ L+ Sbjct: 264 KEHLRSDEKLTRDESKKKSKFQDNDHGHEPDSELD 298 >At4g29000.1 68417.m04145 tesmin/TSO1-like CXC domain-containing protein similar to CXC domain containing TSO1-like protein 1 (SOL1) [Arabidopsis thaliana] GI:7767427, SP|Q9Y4I5 Tesmin (Metallothionein-like 5, testis-specific) {Homo sapiens}; contains Pfam profile PF03638: Tesmin/TSO1-like CXC domain Length = 603 Score = 29.1 bits (62), Expect = 3.0 Identities = 19/66 (28%), Positives = 29/66 (43%), Gaps = 3/66 (4%) Frame = +3 Query: 459 EQLLETTSKTIPH*RNKSDDIMSRAMLHNKIPAEGPTNSN---IDPSKFIEVQPKFLNSE 629 E L ++++ P N + D+ A HN+ GP SN +D SK + P L Sbjct: 402 ETSLASSAQDQPQGNNNAADVEMVATDHNQADKSGPEESNSDGVDASKVTPLSPATLALM 461 Query: 630 ISRQET 647 Q+T Sbjct: 462 CDEQDT 467 >At2g34080.1 68415.m04172 cysteine proteinase, putative contains similarity to cysteine protease SPCP1 GI:13491750 from [Ipomoea batatas] Length = 345 Score = 28.7 bits (61), Expect = 3.9 Identities = 19/67 (28%), Positives = 36/67 (53%) Frame = +3 Query: 291 NMKLDIFRRKLRKLEQLTASKSKKKSKRIGNSESYIPENNLEYLKLATQNKKAIIDEQLL 470 NM+ D+F++ L+ +E +K KS ++G +E + N E+L + T K + + Sbjct: 57 NMRRDVFKKNLKFIENF--NKKGNKSYKLGVNE-FADWTNEEFLAIHT-GLKGLTEVSPS 112 Query: 471 ETTSKTI 491 + +KTI Sbjct: 113 KVVAKTI 119 >At1g02280.1 68414.m00169 GTP-binding protein (TOC33) identical to atToc33 protein (GI:11557973) [Arabidopsis thaliana]; Carboxyl-terminal end highly similar to GTP-binding protein SP:U43377, location of EST gb|AA394770 and gb|R30089; identical to cDNA for chloroplast atToc33 protein GI:11557972 Length = 297 Score = 28.3 bits (60), Expect = 5.2 Identities = 14/43 (32%), Positives = 26/43 (60%), Gaps = 1/43 (2%) Frame = +3 Query: 348 SKSKKKSKRIGNSESYIPENNLEYLKLATQNKKAI-IDEQLLE 473 SK+ K K + N E++IP +AT +KAI +D+++++ Sbjct: 214 SKNDKDEKALPNGEAWIPNLVKAITDVATNQRKAIHVDKKMVD 256 >At5g16160.1 68418.m01889 expressed protein Length = 133 Score = 27.9 bits (59), Expect = 6.8 Identities = 15/71 (21%), Positives = 34/71 (47%) Frame = +3 Query: 282 KQVNMKLDIFRRKLRKLEQLTASKSKKKSKRIGNSESYIPENNLEYLKLATQNKKAIIDE 461 KQ++ ++ +R+++ K K K+ + S++ + + LK T + + ++E Sbjct: 41 KQISKLQELHKRRMQIKANAKIHKKKPKASKNSQSKAIEDGESSKKLKEPTSSSSSTLEE 100 Query: 462 QLLETTSKTIP 494 Q T T+P Sbjct: 101 QNHNKTVVTVP 111 >At4g02650.1 68417.m00360 epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related contains Pfam PF01417: ENTH domain. ENTH (Epsin N-terminal homology) domain; similar to Chain A, Calm-N N-Terminal Domain Of Clathrin Assembly Lymphoid Myeloid Leukaemia Protein, Pi(4,5)p2 Complex (GP:13399999) {Homo sapiens}; supporting cDNA gi|26451912|dbj|AK118440.1| Length = 611 Score = 27.5 bits (58), Expect = 9.0 Identities = 16/50 (32%), Positives = 23/50 (46%) Frame = +3 Query: 276 ISKQVNMKLDIFRRKLRKLEQLTASKSKKKSKRIGNSESYIPENNLEYLK 425 + K KLD+ +R L A +K SKR SE E+ EY++ Sbjct: 312 LEKITQKKLDLMDEFIRDKSALAAQTTKSSSKRSNKSEE--EESKTEYIQ 359 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,994,359 Number of Sequences: 28952 Number of extensions: 213010 Number of successful extensions: 694 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 683 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 694 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1496852856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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