BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0599 (675 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g22753.1 68417.m03284 sterol desaturase family protein simila... 27 8.7 At1g70070.1 68414.m08062 DEAD/DEAH box helicase, putative simila... 27 8.7 >At4g22753.1 68417.m03284 sterol desaturase family protein similar to sterol 4-alpha-methyl-oxidase GI:16973471 from [Arabidopsis thaliana]; contains Pfam profile PF01598: Sterol desaturase Length = 291 Score = 27.5 bits (58), Expect = 8.7 Identities = 15/64 (23%), Positives = 33/64 (51%), Gaps = 5/64 (7%) Frame = +1 Query: 277 YVLENKIKYNM-----CVK*SVYICIAVIGTAEVLRCMLYQLVGMRGHILSLSCQASVVI 441 + ++ K+KY++ C K + + + V+GT +++ Q+VG+R + S V Sbjct: 68 FKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPLPSLMEIVAQ 127 Query: 442 MFIY 453 + +Y Sbjct: 128 LVVY 131 >At1g70070.1 68414.m08062 DEAD/DEAH box helicase, putative similar to SP|P35207 Antiviral protein SKI2 {Saccharomyces cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 1171 Score = 27.5 bits (58), Expect = 8.7 Identities = 13/18 (72%), Positives = 14/18 (77%) Frame = +1 Query: 343 VIGTAEVLRCMLYQLVGM 396 VI T E+LR MLYQ VGM Sbjct: 242 VIMTTEILRNMLYQSVGM 259 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,492,290 Number of Sequences: 28952 Number of extensions: 270119 Number of successful extensions: 424 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 422 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 424 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1432596384 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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