Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0570
(634 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5)... 118 3e-27
At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial, p... 115 2e-26
At5g49910.1 68418.m06180 heat shock protein 70 / HSP70 (HSC70-7)... 108 3e-24
At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70... 107 5e-24
At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1 (HS... 101 5e-22
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS... 101 5e-22
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 100 7e-22
At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS... 99 1e-21
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 99 2e-21
At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)... 99 3e-21
At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70... 98 4e-21
At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)... 98 5e-21
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 89 2e-18
At2g32120.2 68415.m03926 heat shock protein 70 family protein / ... 55 3e-08
At2g32120.1 68415.m03925 heat shock protein 70 family protein / ... 55 3e-08
At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)... 50 1e-06
At3g61570.1 68416.m06896 intracellular protein transport protein... 31 0.48
At2g28830.1 68415.m03505 armadillo/beta-catenin repeat family pr... 30 1.1
At1g51740.1 68414.m05830 syntaxin 81 (SYP81) identical to SP|P59... 29 2.6
At4g11450.1 68417.m01843 expressed protein 28 4.5
At5g53020.1 68418.m06585 expressed protein 28 5.9
At2g46180.1 68415.m05742 intracellular protein transport protein... 27 7.8
At1g60630.1 68414.m06825 leucine-rich repeat family protein simi... 27 7.8
At1g22850.1 68414.m02853 expressed protein 27 7.8
>At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5)
identical to heat shock protein 70 [Arabidopsis
thaliana] GI:6746590
Length = 682
Score = 118 bits (284), Expect = 3e-27
Identities = 57/79 (72%), Positives = 66/79 (83%)
Frame = +2
Query: 2 SDNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVIQ*SGGLS 181
+DNKLLG+F LVGIPP+PRGVPQIEV FDIDANGIV VSA+DK TGK QQI I+ SGGLS
Sbjct: 498 TDNKLLGEFDLVGIPPSPRGVPQIEVTFDIDANGIVTVSAKDKTTGKVQQITIRSSGGLS 557
Query: 182 KDEIENMVKAAEQFAAADK 238
+D+I+ MV+ AE A DK
Sbjct: 558 EDDIQKMVREAELHAQKDK 576
>At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial,
putative / HSP70, mitochondrial, putative strong
similarity to SP|Q01899 Heat shock 70 kDa protein,
mitochondrial precursor {Phaseolus vulgaris}
Length = 682
Score = 115 bits (277), Expect = 2e-26
Identities = 56/79 (70%), Positives = 63/79 (79%)
Frame = +2
Query: 2 SDNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVIQ*SGGLS 181
+DNK+LG+F LVGIPPAPRG+PQIEV FDIDANGI VSA+DK TGKEQ I I+ SGGLS
Sbjct: 493 ADNKVLGEFDLVGIPPAPRGMPQIEVTFDIDANGITTVSAKDKATGKEQNITIRSSGGLS 552
Query: 182 KDEIENMVKAAEQFAAADK 238
DEI MVK AE A D+
Sbjct: 553 DDEINRMVKEAELNAQKDQ 571
>At5g49910.1 68418.m06180 heat shock protein 70 / HSP70 (HSC70-7)
identical to heat shock protein 70 [Arabidopsis
thaliana] GI:6746592
Length = 718
Score = 108 bits (259), Expect = 3e-24
Identities = 52/78 (66%), Positives = 60/78 (76%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVIQ*SGGLSK 184
DNK +G F L GIPPAPRGVPQIEV FDIDANGI+ VSA DKGTGK+Q I I + L K
Sbjct: 521 DNKSIGSFRLDGIPPAPRGVPQIEVKFDIDANGILSVSASDKGTGKKQDITITGASTLPK 580
Query: 185 DEIENMVKAAEQFAAADK 238
DE++ MV+ AE+FA DK
Sbjct: 581 DEVDTMVQEAERFAKEDK 598
>At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein 70
[Arabidopsis thaliana] GI:6746592; similar to heat shock
70 protein - Spinacia oleracea,PID:g2654208
Length = 718
Score = 107 bits (258), Expect = 5e-24
Identities = 53/78 (67%), Positives = 60/78 (76%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVIQ*SGGLSK 184
DNK LG F L GIPPAPRGVPQIEV FDIDANGI+ VSA DKGTGK+Q I I + L K
Sbjct: 521 DNKSLGSFRLDGIPPAPRGVPQIEVKFDIDANGILSVSAVDKGTGKKQDITITGASTLPK 580
Query: 185 DEIENMVKAAEQFAAADK 238
DE++ MV+ AE+FA DK
Sbjct: 581 DEVDQMVQEAERFAKDDK 598
>At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1
(HSC70-1) (HSP70-1) identical to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}
Length = 651
Score = 101 bits (241), Expect = 5e-22
Identities = 50/79 (63%), Positives = 61/79 (77%), Gaps = 1/79 (1%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVI-Q*SGGLS 181
DN LLG+F L GIPPAPRGVPQI V FDIDANGI++VSA DK TG++ +I I G LS
Sbjct: 458 DNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLS 517
Query: 182 KDEIENMVKAAEQFAAADK 238
KDEIE MV+ AE++ + D+
Sbjct: 518 KDEIEKMVQEAEKYKSEDE 536
>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
(HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
thaliana}
Length = 649
Score = 101 bits (241), Expect = 5e-22
Identities = 50/79 (63%), Positives = 61/79 (77%), Gaps = 1/79 (1%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVI-Q*SGGLS 181
DN LLG+F L GIPPAPRGVPQI V FDIDANGI++VSA DK TG++ +I I G LS
Sbjct: 458 DNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLS 517
Query: 182 KDEIENMVKAAEQFAAADK 238
KDEIE MV+ AE++ + D+
Sbjct: 518 KDEIEKMVQEAEKYKSEDE 536
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 100 bits (240), Expect = 7e-22
Identities = 50/79 (63%), Positives = 61/79 (77%), Gaps = 1/79 (1%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVI-Q*SGGLS 181
DN LLG+F L GIPPAPRGVPQI V FDIDANGI++VSA DK TG++ +I I G LS
Sbjct: 458 DNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLS 517
Query: 182 KDEIENMVKAAEQFAAADK 238
K+EIE MV+ AE++ A D+
Sbjct: 518 KEEIEKMVQEAEKYKAEDE 536
>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
(HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
thaliana}
Length = 653
Score = 99 bits (238), Expect = 1e-21
Identities = 49/79 (62%), Positives = 61/79 (77%), Gaps = 1/79 (1%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVI-Q*SGGLS 181
DN LLG+F L GIPPAPRGVPQI V FDIDANGI++VSA DK TGK+ +I I G LS
Sbjct: 458 DNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGKKNKITITNDKGRLS 517
Query: 182 KDEIENMVKAAEQFAAADK 238
K++IE MV+ AE++ + D+
Sbjct: 518 KEDIEKMVQEAEKYKSEDE 536
>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
putative / HSC70, putative / HSP70, putative strong
similarity to heat shock cognate 70 kd protein 1
SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
Length = 617
Score = 99.1 bits (236), Expect = 2e-21
Identities = 47/79 (59%), Positives = 60/79 (75%), Gaps = 1/79 (1%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVI-Q*SGGLS 181
DN +LGQF L GIPPAPRG+PQ V FDID+NGI++VSA DK TGK+ +I I G LS
Sbjct: 458 DNNILGQFVLSGIPPAPRGIPQFTVCFDIDSNGILNVSAEDKATGKKNKITITNDKGRLS 517
Query: 182 KDEIENMVKAAEQFAAADK 238
KD+IE MV+ AE++ + D+
Sbjct: 518 KDDIEKMVQEAEKYKSEDE 536
>At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 668
Score = 98.7 bits (235), Expect = 3e-21
Identities = 46/79 (58%), Positives = 60/79 (75%), Gaps = 1/79 (1%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVI-Q*SGGLS 181
D +LLG+F L G+PPAPRG PQIEV F++DANGI++V A DK +GK ++I I G LS
Sbjct: 483 DCRLLGKFDLTGVPPAPRGTPQIEVTFEVDANGILNVKAEDKASGKSEKITITNEKGRLS 542
Query: 182 KDEIENMVKAAEQFAAADK 238
++EI+ MVK AE+FA DK
Sbjct: 543 QEEIDRMVKEAEEFAEEDK 561
>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
putative similar to heat shock protein hsp70 GI:1771478
from [Pisum sativum]
Length = 646
Score = 98.3 bits (234), Expect = 4e-21
Identities = 50/79 (63%), Positives = 58/79 (73%), Gaps = 1/79 (1%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVI-Q*SGGLS 181
DN LLG F L GIPPAPRGVPQI V FDIDANGI++VSA DK G + QI I G LS
Sbjct: 457 DNNLLGTFELKGIPPAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQITITNDKGRLS 516
Query: 182 KDEIENMVKAAEQFAAADK 238
K+EIE MV+ AE++ A D+
Sbjct: 517 KEEIEKMVQDAEKYKAEDE 535
>At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)
SWISS-PROT:Q9LKR3 PMID:8888624
Length = 669
Score = 97.9 bits (233), Expect = 5e-21
Identities = 47/79 (59%), Positives = 60/79 (75%), Gaps = 1/79 (1%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVI-Q*SGGLS 181
D +LLG+F L GIPPAPRG PQIEV F++DANGI++V A DK +GK ++I I G LS
Sbjct: 483 DCRLLGKFDLNGIPPAPRGTPQIEVTFEVDANGILNVKAEDKASGKSEKITITNEKGRLS 542
Query: 182 KDEIENMVKAAEQFAAADK 238
++EI+ MVK AE+FA DK
Sbjct: 543 QEEIDRMVKEAEEFAEEDK 561
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 89.0 bits (211), Expect = 2e-18
Identities = 44/79 (55%), Positives = 55/79 (69%), Gaps = 1/79 (1%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIVI-Q*SGGLS 181
DN+ LG+F L GI PAPRGVPQIEV F++DANGI+ V A DK Q I I G L+
Sbjct: 497 DNRELGKFDLTGILPAPRGVPQIEVTFEVDANGILQVKAEDKVAKTSQSITITNDKGRLT 556
Query: 182 KDEIENMVKAAEQFAAADK 238
++EIE M++ AE+FA DK
Sbjct: 557 EEEIEEMIREAEEFAEEDK 575
>At2g32120.2 68415.m03926 heat shock protein 70 family protein /
HSP70 family protein similar to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}; contains InterPro accession IPR001023: Heat
shock protein Hsp70
Length = 563
Score = 55.2 bits (127), Expect = 3e-08
Identities = 26/51 (50%), Positives = 32/51 (62%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIV 157
+N LLG F LVGIPPAP+GVP+I V DIDA+ + V A G +V
Sbjct: 475 ENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVV 525
>At2g32120.1 68415.m03925 heat shock protein 70 family protein /
HSP70 family protein similar to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}; contains InterPro accession IPR001023: Heat
shock protein Hsp70
Length = 563
Score = 55.2 bits (127), Expect = 3e-08
Identities = 26/51 (50%), Positives = 32/51 (62%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFDIDANGIVHVSARDKGTGKEQQIV 157
+N LLG F LVGIPPAP+GVP+I V DIDA+ + V A G +V
Sbjct: 475 ENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVV 525
>At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 613
Score = 50.0 bits (114), Expect = 1e-06
Identities = 29/72 (40%), Positives = 40/72 (55%), Gaps = 2/72 (2%)
Frame = +2
Query: 5 DNKLLGQFSLVGIPPAPRGVPQIEVAFD-IDANGIVHVSARD-KGTGKEQQIVIQ*SGGL 178
D +LLG+F L G+PPAPRG PQIEV F+ IDA + + K ++ + G
Sbjct: 483 DCRLLGKFDLTGVPPAPRGTPQIEVTFEKIDARNALETYVYNMKNQVSDKDKLADKLEGD 542
Query: 179 SKDEIENMVKAA 214
K++IE K A
Sbjct: 543 EKEKIEAATKEA 554
>At3g61570.1 68416.m06896 intracellular protein transport protein
USO1-related contains weak similarity to intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]
Length = 712
Score = 31.5 bits (68), Expect = 0.48
Identities = 22/72 (30%), Positives = 40/72 (55%), Gaps = 10/72 (13%)
Frame = +3
Query: 330 QDECDKLREEMNKLRELLAKKDTIEP----------EELRTATGQLQQASLKLFEQAYKK 479
Q E DK R+E+ +L++ L +K+T E EELR T + Q++ + E++ K+
Sbjct: 359 QRERDKARQELKRLKQHLLEKETEESEKMDEDSRLIEELR-QTNEYQRSQISHLEKSLKQ 417
Query: 480 MAAEREGSQHSN 515
+ +E ++ SN
Sbjct: 418 AISNQEDNRLSN 429
>At2g28830.1 68415.m03505 armadillo/beta-catenin repeat family
protein / U-box domain-containing protein contains Pfam
domain, PF00514: Armadillo/beta-catenin-like repeats and
Pfam, PF04564: U-box domain
Length = 654
Score = 30.3 bits (65), Expect = 1.1
Identities = 12/29 (41%), Positives = 21/29 (72%)
Frame = +3
Query: 354 EEMNKLRELLAKKDTIEPEELRTATGQLQ 440
+E NK+ ELL K + +PE+ R+A G+++
Sbjct: 352 DEHNKIEELLLKLTSQQPEDRRSAAGEIR 380
>At1g51740.1 68414.m05830 syntaxin 81 (SYP81) identical to SP|P59277
Syntaxin 81 (AtSYP81) {Arabidopsis thaliana}; identified
as syntaxin SYP81 by Sanderfoot, A.A., et al in Plant
Physiol. 124:1558-69 (2000); similar to Syntaxin 18
(SP:Q9P2W9){Homo sapiens}
Length = 310
Score = 29.1 bits (62), Expect = 2.6
Identities = 15/53 (28%), Positives = 29/53 (54%)
Frame = +3
Query: 294 RHQDGRVQGALPQDECDKLREEMNKLRELLAKKDTIEPEELRTATGQLQQASL 452
R QD + A+P+ + ++ +E + L ++IEP+E++ +LQQ L
Sbjct: 159 RFQD-IINRAMPRRKPKRVIKEATPINTTLGNSESIEPDEIQAQPRRLQQQQL 210
>At4g11450.1 68417.m01843 expressed protein
Length = 694
Score = 28.3 bits (60), Expect = 4.5
Identities = 12/19 (63%), Positives = 15/19 (78%), Gaps = 1/19 (5%)
Frame = -2
Query: 60 PRGAGGMPTSEN-CPSSLL 7
PRG G+PT+EN CPS L+
Sbjct: 517 PRGNHGLPTTENSCPSPLI 535
>At5g53020.1 68418.m06585 expressed protein
Length = 721
Score = 27.9 bits (59), Expect = 5.9
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = +3
Query: 336 ECDKLREEMNKLRELLAKKDTIEPEELRTATGQLQQASLKLFEQAYKKMAAE 491
E + L E+NK+R+ L +KD I ++ + + + + L ++A KK E
Sbjct: 195 ELEDLSLEINKMRKDLEQKDRILAVMMKKSKLDMTEKQMTLLKEAKKKQDEE 246
>At2g46180.1 68415.m05742 intracellular protein transport protein
USO1-related similar to Intracellular protein transport
protein USO1 (Swiss-Prot:P25386) [Saccharomyces
cerevisiae]
Length = 725
Score = 27.5 bits (58), Expect = 7.8
Identities = 11/27 (40%), Positives = 20/27 (74%)
Frame = +3
Query: 336 ECDKLREEMNKLRELLAKKDTIEPEEL 416
E DK R+E+ +L++ L +K+T E E++
Sbjct: 366 EKDKARQELKRLKQHLLEKETEESEKM 392
>At1g60630.1 68414.m06825 leucine-rich repeat family protein similar
to receptor kinase GI:498278 from [Petunia
integrifolia]; contains protein kinase domain,
Pfam:PF00069; contains leucine-rich repeats,
Pfam:PF00560
Length = 652
Score = 27.5 bits (58), Expect = 7.8
Identities = 14/40 (35%), Positives = 17/40 (42%)
Frame = -2
Query: 255 PRSRRVLSAAANCSAAFTMFSISSFDSPPDYWITICCSLP 136
P R VL + A +FS +S D P W SLP
Sbjct: 606 PAMREVLKMVKDARAEAALFSFNSSDHSPGRWSDTIQSLP 645
>At1g22850.1 68414.m02853 expressed protein
Length = 344
Score = 27.5 bits (58), Expect = 7.8
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +1
Query: 478 RWQLNAKEANTATAADGSDAEPREEGGEELDGFLYG 585
RW N+K N D +E ++GG E D + G
Sbjct: 73 RWFFNSKSTNDENDEDDVKSESDDDGGSEGDAAIKG 108
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,976,125
Number of Sequences: 28952
Number of extensions: 154397
Number of successful extensions: 627
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 605
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 619
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1295224128
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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