Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0562
(730 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 132 3e-31
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 50 1e-06
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 50 1e-06
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 35 0.064
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 35 0.064
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 29 2.4
At5g13340.1 68418.m01535 expressed protein 29 4.2
At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase fa... 29 4.2
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 29 4.2
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 29 4.2
At3g19000.2 68416.m02412 oxidoreductase, 2OG-Fe(II) oxygenase fa... 29 4.2
At3g19000.1 68416.m02411 oxidoreductase, 2OG-Fe(II) oxygenase fa... 29 4.2
At5g45720.1 68418.m05621 hypothetical protein 28 5.5
At5g40740.1 68418.m04944 expressed protein 28 7.3
At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P329... 27 9.6
At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolas... 27 9.6
At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to ... 27 9.6
At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to ... 27 9.6
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 132 bits (318), Expect = 3e-31
Identities = 57/73 (78%), Positives = 64/73 (87%)
Frame = +3
Query: 510 VIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPV 689
VIVS ILERD H ++LWNTAV+I + GN+IGKHRKNHIPRVGDFNES YYMEG+TGHPV
Sbjct: 177 VIVSPILERDIDHGEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPV 236
Query: 690 FATRYGKIAVNIC 728
F T +GKIAVNIC
Sbjct: 237 FETVFGKIAVNIC 249
Score = 109 bits (263), Expect = 1e-24
Identities = 50/83 (60%), Positives = 64/83 (77%)
Frame = +1
Query: 256 IVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPW 435
++Q+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W MPFAFCTRE++ W
Sbjct: 94 LIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTMPFAFCTRERR-W 152
Query: 436 CEFAESAEDGPTTTFLRELAIKY 504
CEFAE DG +T FL+ELA KY
Sbjct: 153 CEFAEPV-DGESTKFLQELAKKY 174
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 50.4 bits (115), Expect = 1e-06
Identities = 22/56 (39%), Positives = 33/56 (58%)
Frame = +3
Query: 561 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNIC 728
+N+ +I G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC
Sbjct: 131 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAIC 186
Score = 31.1 bits (67), Expect = 0.78
Identities = 20/81 (24%), Positives = 40/81 (49%)
Frame = +1
Query: 253 RIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP 432
R V S ++P + + F ++ A +G NII QEL+ + FC +++
Sbjct: 32 RFVSLSSSLPLSNYQSLPSSSSFKFPYALVREAHAKGANIILIQELFE-GYYFCQAQRED 90
Query: 433 WCEFAESAEDGPTTTFLRELA 495
+ + A+ ++ PT +++LA
Sbjct: 91 FFKRAKPYKNHPTIARMQKLA 111
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 50.4 bits (115), Expect = 1e-06
Identities = 22/56 (39%), Positives = 33/56 (58%)
Frame = +3
Query: 561 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNIC 728
+N+ +I G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC
Sbjct: 104 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAIC 159
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 34.7 bits (76), Expect = 0.064
Identities = 24/68 (35%), Positives = 30/68 (44%), Gaps = 5/68 (7%)
Frame = +3
Query: 540 EKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRY 704
E+ D L+NT V G + KHRK H IP F ES G T + T
Sbjct: 176 ERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDTDV 234
Query: 705 GKIAVNIC 728
G+I + IC
Sbjct: 235 GRIGIGIC 242
Score = 33.5 bits (73), Expect = 0.15
Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +1
Query: 295 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 468
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 469 TTTFLRELA 495
+T L E++
Sbjct: 154 STAMLSEVS 162
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 34.7 bits (76), Expect = 0.064
Identities = 24/68 (35%), Positives = 30/68 (44%), Gaps = 5/68 (7%)
Frame = +3
Query: 540 EKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRY 704
E+ D L+NT V G + KHRK H IP F ES G T + T
Sbjct: 176 ERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDTDV 234
Query: 705 GKIAVNIC 728
G+I + IC
Sbjct: 235 GRIGIGIC 242
Score = 33.5 bits (73), Expect = 0.15
Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +1
Query: 295 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 468
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 469 TTTFLRELA 495
+T L E++
Sbjct: 154 STAMLSEVS 162
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 29.5 bits (63), Expect = 2.4
Identities = 21/63 (33%), Positives = 28/63 (44%)
Frame = +3
Query: 540 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 719
EK L+ TA+ S G +GKHRK +P + + PV+ T GKI
Sbjct: 127 EKDGYTLYCTALFFSPQGQFLGKHRK-VMPT--SLERCIWGQGDGSTIPVYDTPIGKIGA 183
Query: 720 NIC 728
IC
Sbjct: 184 AIC 186
>At5g13340.1 68418.m01535 expressed protein
Length = 242
Score = 28.7 bits (61), Expect = 4.2
Identities = 17/50 (34%), Positives = 25/50 (50%)
Frame = +3
Query: 162 SLQLRRLTSTSPHTLSRPRTSRPDPREL*R*NSSAFHRGAHRSSSQRAKE 311
S + RR S SP+T ++ P PR+ R SS+ HR + + KE
Sbjct: 27 SRRTRRDRSRSPYTSRHKKSRSPAPRQHQRDRSSSLSPSEHRIAIEVKKE 76
>At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase
family protein low similarity to 55 kDa type II
phosphatidylinositol 4-kinase [Rattus norvegicus]
GI:13660755; contains Pfam profile PF00454:
Phosphatidylinositol 3- and 4-kinase
Length = 533
Score = 28.7 bits (61), Expect = 4.2
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = -3
Query: 554 VRMLLVPF*YRGHDHLAYLMASSRRKV 474
VR+ VPF RG DH AY +AS +R V
Sbjct: 187 VRISHVPFHDRGSDHAAYKVASLQRFV 213
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 28.7 bits (61), Expect = 4.2
Identities = 22/75 (29%), Positives = 32/75 (42%)
Frame = +3
Query: 504 RKVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGH 683
RK V ++ EK L+ T + S G +GKHRK +P + +
Sbjct: 115 RKNHVYLVMGAIEKEGYTLYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTI 171
Query: 684 PVFATRYGKIAVNIC 728
PV+ T GK+ IC
Sbjct: 172 PVYDTPIGKLGAAIC 186
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 28.7 bits (61), Expect = 4.2
Identities = 20/63 (31%), Positives = 28/63 (44%)
Frame = +3
Query: 540 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 719
EK L+ TA+ S G +GKHRK +P + + PV+ T GK+
Sbjct: 120 EKDGYTLYCTALFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 176
Query: 720 NIC 728
IC
Sbjct: 177 AIC 179
>At3g19000.2 68416.m02412 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to SP|P24397 Hyoscyamine
6-dioxygenase (EC 1.14.11.11) (Hyoscyamine 6-beta-
hydroxylase) {Hyoscyamus niger}, SP|Q05965
Naringenin,2-oxoglutarate 3-dioxygenase (EC 1.14.11.9)
(Flavonone- 3-hydroxylase) (F3H) (FHT) {Matthiola
incana}; contains Pfam profile PF03171: oxidoreductase,
2OG-Fe(II) oxygenase family
Length = 278
Score = 28.7 bits (61), Expect = 4.2
Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 2/72 (2%)
Frame = +3
Query: 468 DHDLPSGTRHQVRKVIVSSI-LERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDF 644
+H LPS RH+V K L +EK V D N +G H + H V D+
Sbjct: 68 NHGLPSALRHRVEKTAAEFFNLTTEEKRK--------VKRDEVNPMGYHDEEHTKNVRDW 119
Query: 645 NE-SNYYMEGNT 677
E +++++ +T
Sbjct: 120 KEIFDFFLQDST 131
>At3g19000.1 68416.m02411 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to SP|P24397 Hyoscyamine
6-dioxygenase (EC 1.14.11.11) (Hyoscyamine 6-beta-
hydroxylase) {Hyoscyamus niger}, SP|Q05965
Naringenin,2-oxoglutarate 3-dioxygenase (EC 1.14.11.9)
(Flavonone- 3-hydroxylase) (F3H) (FHT) {Matthiola
incana}; contains Pfam profile PF03171: oxidoreductase,
2OG-Fe(II) oxygenase family
Length = 352
Score = 28.7 bits (61), Expect = 4.2
Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 2/72 (2%)
Frame = +3
Query: 468 DHDLPSGTRHQVRKVIVSSI-LERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDF 644
+H LPS RH+V K L +EK V D N +G H + H V D+
Sbjct: 68 NHGLPSALRHRVEKTAAEFFNLTTEEKRK--------VKRDEVNPMGYHDEEHTKNVRDW 119
Query: 645 NE-SNYYMEGNT 677
E +++++ +T
Sbjct: 120 KEIFDFFLQDST 131
>At5g45720.1 68418.m05621 hypothetical protein
Length = 900
Score = 28.3 bits (60), Expect = 5.5
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = +3
Query: 576 VISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATR 701
V+ D+ IG+H +NHI + N+ N PV R
Sbjct: 9 VLKDSNGDIGEHLRNHIHLTNCIHLKNHMHNNNKQSPVLTDR 50
>At5g40740.1 68418.m04944 expressed protein
Length = 741
Score = 27.9 bits (59), Expect = 7.3
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +3
Query: 531 ERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESN 656
ER+ + ++ +++ NVIG N PRVG F SN
Sbjct: 8 ERELELESAMYTNCLLLGLDPNVIGLGASNGTPRVGLFRHSN 49
>At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 224
Score = 27.5 bits (58), Expect = 9.6
Identities = 19/63 (30%), Positives = 27/63 (42%)
Frame = +3
Query: 540 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 719
EK L+ T + S G +GKHRK +P + + PV+ T GK+
Sbjct: 5 EKEGYTLYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 61
Query: 720 NIC 728
IC
Sbjct: 62 AIC 64
>At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 422
Score = 27.5 bits (58), Expect = 9.6
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = -2
Query: 636 RLSECGSCDVSRSRFRCH*LQPQCSKVCPNASRPFLISRTRSP 508
RL G C + R CH ++ SK R LI + R+P
Sbjct: 332 RLQGVGQCLIREYRMVCHVMKGDISKDFVEGCRAVLIDKDRNP 374
>At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 383
Score = 27.5 bits (58), Expect = 9.6
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +3
Query: 579 ISDTGNVIGKHRKNHIP 629
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
>At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 250
Score = 27.5 bits (58), Expect = 9.6
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +3
Query: 579 ISDTGNVIGKHRKNHIP 629
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,540,345
Number of Sequences: 28952
Number of extensions: 354109
Number of successful extensions: 1121
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1085
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1119
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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