Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0559
(683 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 72 9e-14
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 52 6e-08
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 52 6e-08
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 52 6e-08
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 43 5e-05
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 33 0.051
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.20
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 30 0.36
SPBC557.05 |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 30 0.36
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.36
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 29 0.83
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 29 0.83
SPAC4G9.09c |arg11||N-acetyl-gamma-glutamyl-phosphate reductase/... 28 1.1
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 1.9
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 27 3.3
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 27 3.3
SPAC589.11 |mug82||translation release factor |Schizosaccharomyc... 27 3.3
SPAC227.14 |||nicotinamide riboside kinase|Schizosaccharomyces p... 25 7.7
SPBC1289.12 |usp109||U1 snRNP-associated protein Usp109|Schizosa... 25 7.7
SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces... 25 7.7
SPAC630.14c |tup12||transcriptional corepressor Tup12 |Schizosac... 25 7.7
SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein Rrp12|Sch... 25 7.7
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 71.7 bits (168), Expect = 9e-14
Identities = 31/37 (83%), Positives = 35/37 (94%)
Frame = +2
Query: 569 KEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEK 679
KEHVN+VFIGHVDAGKST+GG I+ LTGMVDKRT+EK
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEK 272
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 52.4 bits (120), Expect = 6e-08
Identities = 22/39 (56%), Positives = 30/39 (76%)
Frame = +2
Query: 566 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKY 682
+K H+NVV IGHVD+GKST G ++ G +DKRT+EK+
Sbjct: 4 EKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKF 42
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 52.4 bits (120), Expect = 6e-08
Identities = 22/39 (56%), Positives = 30/39 (76%)
Frame = +2
Query: 566 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKY 682
+K H+NVV IGHVD+GKST G ++ G +DKRT+EK+
Sbjct: 4 EKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKF 42
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 52.4 bits (120), Expect = 6e-08
Identities = 22/39 (56%), Positives = 30/39 (76%)
Frame = +2
Query: 566 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKY 682
+K H+NVV IGHVD+GKST G ++ G +DKRT+EK+
Sbjct: 4 EKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKF 42
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 42.7 bits (96), Expect = 5e-05
Identities = 23/60 (38%), Positives = 37/60 (61%), Gaps = 6/60 (10%)
Frame = +2
Query: 518 KKIPKKKP------PRVEDTRSKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEK 679
KKI K+ P P + + + K V++V GHVD+GKST+ G+IM G ++ R+++K
Sbjct: 152 KKIKKQNPTDLVSVPEIFEQSNPKPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQK 211
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 32.7 bits (71), Expect = 0.051
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = +2
Query: 566 KKEHVNVVFIGHVDAGKSTIGGQI 637
KK HVN+ IGHVD GK+T+ I
Sbjct: 50 KKPHVNIGTIGHVDHGKTTLTAAI 73
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.7 bits (66), Expect = 0.20
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +2
Query: 581 NVVFIGHVDAGKSTIGGQIMSLTGMVDK 664
N I H+D GKST+ I+ LTG++++
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINE 87
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 29.9 bits (64), Expect = 0.36
Identities = 16/45 (35%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = +2
Query: 515 TKKIPKKKPPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSL 646
+KK+ K ++E+ K + + V+ +G D+GKSTI QI L
Sbjct: 13 SKKLNSKIEKQIENASKKDKKIYKVLLLGASDSGKSTISKQIKIL 57
>SPBC557.05 |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 433
Score = 29.9 bits (64), Expect = 0.36
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +1
Query: 547 SRRYTKQKRTCKCSIYRTCRCRKVDYWWSNNVTHWNG 657
SR+ ++ +TCKC T RC K +N +WNG
Sbjct: 289 SRKNSEVSKTCKCLQKETIRCNK-----KSNCYNWNG 320
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 29.9 bits (64), Expect = 0.36
Identities = 11/49 (22%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = +2
Query: 524 IPKKKPPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSLTGMVDKR 667
+P P ++ + +E++ N + HVD GK+T+ +++ G++ +
Sbjct: 1 MPVIPPEKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSK 49
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 28.7 bits (61), Expect = 0.83
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +2
Query: 539 PPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSLTGMV 658
P V + K +V N+ I HVD GKST+ ++ G++
Sbjct: 6 PEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII 46
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 28.7 bits (61), Expect = 0.83
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +2
Query: 539 PPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSLTGMV 658
P V + K +V N+ I HVD GKST+ ++ G++
Sbjct: 6 PEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII 46
>SPAC4G9.09c |arg11||N-acetyl-gamma-glutamyl-phosphate
reductase/acetylglutamate kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 885
Score = 28.3 bits (60), Expect = 1.1
Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = +1
Query: 124 GAKDSNDVSSKISTLNVNAMEFVPSFS-KPSQASDSTDSPTSPQ-NQK 261
G KD N +S I + A + + S KP AS +T++P+S Q NQK
Sbjct: 477 GVKDLNKISKFIQSDKPFADAIIQTQSTKPPTASSTTNNPSSSQINQK 524
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.5 bits (58), Expect = 1.9
Identities = 19/72 (26%), Positives = 35/72 (48%), Gaps = 1/72 (1%)
Frame = +1
Query: 52 SATIMSNNGAPDSWENEAEIIGEKGAKD-SNDVSSKISTLNVNAMEFVPSFSKPSQASDS 228
S+TI S++ +P S + K + S+ VSS ST + S S+P+ +S
Sbjct: 340 SSTISSSSSSPSSSSFSSTTSSSKSSSSFSSTVSSSSSTSSSTLTSSSSSSSRPASSSSH 399
Query: 229 TDSPTSPQNQKA 264
+ S +S ++ +
Sbjct: 400 SSSLSSHKSSSS 411
Score = 26.6 bits (56), Expect = 3.3
Identities = 21/65 (32%), Positives = 34/65 (52%)
Frame = +1
Query: 52 SATIMSNNGAPDSWENEAEIIGEKGAKDSNDVSSKISTLNVNAMEFVPSFSKPSQASDST 231
S+TI S++ + S + + I + S+ SS STL+ ++M SFS +S ST
Sbjct: 287 SSTISSSSSSSSSPTSTSSTI----SSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSST 342
Query: 232 DSPTS 246
S +S
Sbjct: 343 ISSSS 347
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 26.6 bits (56), Expect = 3.3
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 563 SKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEK 679
S++ +N+ IGHV GKST+ I + + K LE+
Sbjct: 19 SRQATINIGTIGHVAHGKSTVVKAISGVHTVRFKNELER 57
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 26.6 bits (56), Expect = 3.3
Identities = 10/35 (28%), Positives = 20/35 (57%)
Frame = +2
Query: 554 DTRSKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMV 658
D + K+ N+ H+D+GK+T +++ TG +
Sbjct: 52 DKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI 86
>SPAC589.11 |mug82||translation release factor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 182
Score = 26.6 bits (56), Expect = 3.3
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +1
Query: 502 RMGINKKDSKEKTTTSRRYTKQKRT 576
R+ I KK+S EK + ++Y ++K+T
Sbjct: 151 RISILKKESNEKRLSEKKYKQKKKT 175
>SPAC227.14 |||nicotinamide riboside kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 235
Score = 25.4 bits (53), Expect = 7.7
Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = -1
Query: 176 FTFNVEIFED-TSLESFAPFSPIISASFSHESGAPLLDIIVAERKINNLVFFPG 18
+TF+ ++F L + + SF H G P++D I E K N ++ F G
Sbjct: 94 WTFDADLFYSLVRLMKKITDRELYAPSFDHAIGDPVVDDICVEPK-NRILIFEG 146
>SPBC1289.12 |usp109||U1 snRNP-associated protein
Usp109|Schizosaccharomyces pombe|chr 2|||Manual
Length = 352
Score = 25.4 bits (53), Expect = 7.7
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = -1
Query: 131 FAPFSPIISASFSHESGAPLL 69
F+P +P + S++H + APLL
Sbjct: 282 FSPINPYYAKSWNHTASAPLL 302
>SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 609
Score = 25.4 bits (53), Expect = 7.7
Identities = 11/46 (23%), Positives = 24/46 (52%)
Frame = +1
Query: 436 LMMHYSHLKKTMNRKKKNWIHKRMGINKKDSKEKTTTSRRYTKQKR 573
L + Y+H+ + K +N + + N + +K + + +YTK+ R
Sbjct: 96 LKVEYTHIVERYKNKLENGVDQIHHSNNEKAKIRFSKIEKYTKETR 141
>SPAC630.14c |tup12||transcriptional corepressor Tup12
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 586
Score = 25.4 bits (53), Expect = 7.7
Identities = 9/28 (32%), Positives = 14/28 (50%)
Frame = +2
Query: 506 WELTKKIPKKKPPRVEDTRSKKEHVNVV 589
W +T +P K+PP EH +V+
Sbjct: 263 WTVTSNVPNKEPPISVQLLHTLEHTSVI 290
>SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein
Rrp12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1001
Score = 25.4 bits (53), Expect = 7.7
Identities = 14/38 (36%), Positives = 17/38 (44%)
Frame = +1
Query: 481 KKNWIHKRMGINKKDSKEKTTTSRRYTKQKRTCKCSIY 594
KKNW KK K T +SR K +CS+Y
Sbjct: 355 KKNWATVLSNGIKKVIKTITASSRHANYAKALPRCSVY 392
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,231,430
Number of Sequences: 5004
Number of extensions: 38338
Number of successful extensions: 197
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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