Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0559
(683 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 68 7e-12
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 48 4e-06
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 48 4e-06
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 48 4e-06
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 48 4e-06
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 42 3e-04
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 38 0.008
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 35 0.044
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 33 0.23
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 32 0.41
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 32 0.41
At5g56620.1 68418.m07069 no apical meristem (NAM) family protein... 31 0.71
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 31 0.71
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 29 3.8
At1g19510.1 68414.m02430 myb family transcription factor contain... 28 5.0
At1g04700.1 68414.m00467 protein kinase family protein low simil... 28 5.0
At2g47860.2 68415.m05974 phototropic-responsive NPH3 family prot... 28 6.6
At2g47860.1 68415.m05973 phototropic-responsive NPH3 family prot... 28 6.6
At4g36570.1 68417.m05192 myb family transcription factor conatin... 27 8.8
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 27 8.8
At1g66880.1 68414.m07601 serine/threonine protein kinase family ... 27 8.8
At1g33770.1 68414.m04174 protein kinase family protein contains ... 27 8.8
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 27 8.8
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 67.7 bits (158), Expect = 7e-12
Identities = 32/55 (58%), Positives = 42/55 (76%)
Frame = +2
Query: 518 KKIPKKKPPRVEDTRSKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKY 682
+K K++ V + +KK H+NVVFIGHVDAGKSTIGGQI+ L+G VD R ++KY
Sbjct: 83 EKAAKEEAEDVAEA-NKKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKY 136
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 48.4 bits (110), Expect = 4e-06
Identities = 19/39 (48%), Positives = 29/39 (74%)
Frame = +2
Query: 566 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKY 682
+K H+N+V IGHVD+GKST G ++ G +DKR +E++
Sbjct: 4 EKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERF 42
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 48.4 bits (110), Expect = 4e-06
Identities = 19/39 (48%), Positives = 29/39 (74%)
Frame = +2
Query: 566 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKY 682
+K H+N+V IGHVD+GKST G ++ G +DKR +E++
Sbjct: 4 EKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERF 42
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 48.4 bits (110), Expect = 4e-06
Identities = 19/39 (48%), Positives = 29/39 (74%)
Frame = +2
Query: 566 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKY 682
+K H+N+V IGHVD+GKST G ++ G +DKR +E++
Sbjct: 4 EKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERF 42
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 48.4 bits (110), Expect = 4e-06
Identities = 19/39 (48%), Positives = 29/39 (74%)
Frame = +2
Query: 566 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKY 682
+K H+N+V IGHVD+GKST G ++ G +DKR +E++
Sbjct: 4 EKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERF 42
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 42.3 bits (95), Expect = 3e-04
Identities = 14/35 (40%), Positives = 26/35 (74%)
Frame = +2
Query: 578 VNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKY 682
+N+ +GHVD+GKST+ G+++ L G + ++ + KY
Sbjct: 240 LNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKY 274
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 37.5 bits (83), Expect = 0.008
Identities = 17/44 (38%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = +2
Query: 536 KPPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSLTGMVDK 664
K P ++ T+ E + N I H+D GKST+ ++M LTG + K
Sbjct: 52 KEPTIDLTKFPSEKIRNFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 35.1 bits (77), Expect = 0.044
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +2
Query: 566 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKY 682
KK HVN+ IGHVD GK+T+ + + +KY
Sbjct: 76 KKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKY 114
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 32.7 bits (71), Expect = 0.23
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +2
Query: 581 NVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEK 679
N I H+D GKST+ +++ +TG V R +++
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKE 120
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 31.9 bits (69), Expect = 0.41
Identities = 20/57 (35%), Positives = 29/57 (50%)
Frame = +2
Query: 509 ELTKKIPKKKPPRVEDTRSKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEK 679
+LTK K P + S++ +N+ IGHV GKSTI I + + K LE+
Sbjct: 17 DLTKLDVTKLHPLFPEVISRQATINIGTIGHVAHGKSTIVKAISGVQTVRFKNELER 73
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 31.9 bits (69), Expect = 0.41
Identities = 20/57 (35%), Positives = 29/57 (50%)
Frame = +2
Query: 509 ELTKKIPKKKPPRVEDTRSKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEK 679
+LTK K P + S++ +N+ IGHV GKSTI I + + K LE+
Sbjct: 17 DLTKLDVTKLHPLFPEVISRQATINIGTIGHVAHGKSTIVKAISGVQTVRFKNELER 73
>At5g56620.1 68418.m07069 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
Length = 386
Score = 31.1 bits (67), Expect = 0.71
Identities = 18/66 (27%), Positives = 36/66 (54%), Gaps = 3/66 (4%)
Frame = +1
Query: 391 SRPMSRPLQTAGKLRLMMHYSHLKKTMNRKKKNWI-HK-RMGINKKDSKEKTTTSR-RYT 561
+RP+ Q G ++++ Y++ K +K NW+ H+ +GIN+++ + + S+ Y
Sbjct: 154 TRPVMINGQQRGCKKILVLYTNFGKNRRPEKTNWVMHQYHLGINEEEREGELVVSKIFYQ 213
Query: 562 KQKRTC 579
Q R C
Sbjct: 214 TQPRQC 219
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 31.1 bits (67), Expect = 0.71
Identities = 14/23 (60%), Positives = 16/23 (69%)
Frame = +2
Query: 569 KEHVNVVFIGHVDAGKSTIGGQI 637
K HVNV IGHVD GK+T+ I
Sbjct: 65 KPHVNVGTIGHVDHGKTTLTAAI 87
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 28.7 bits (61), Expect = 3.8
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +2
Query: 569 KEHVNVVFIGHVDAGKSTIGGQIMSLTG 652
K++ N+ + H+DAGK+T +I+ TG
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
>At1g19510.1 68414.m02430 myb family transcription factor contains
PFAM profile: PF00249 myb-like DNA binding domain
Length = 100
Score = 28.3 bits (60), Expect = 5.0
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = +1
Query: 25 KKTKLFILRSATIMSNNGAPDSWENEAEIIGEKGAKD 135
K+ K+F A + + PD W+N A+ +G K A++
Sbjct: 15 KQNKMF--ERALAVYDKDTPDRWQNVAKAVGSKSAEE 49
>At1g04700.1 68414.m00467 protein kinase family protein low
similarity to EDR1 [Arabidopsis thaliana] GI:11127925;
contains Pfam profile: PF00069 Eukaryotic protein kinase
domain
Length = 1042
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = +1
Query: 130 KDSNDVSSKISTLNVNAMEFVPSFSKPSQASDSTDSPTSP 249
KDSN + N NA+ F+P P + PTSP
Sbjct: 308 KDSNSPTFMKPYGNTNAVHFMPKMQIPRNSFGQQSPPTSP 347
>At2g47860.2 68415.m05974 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 517
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1
Query: 517 KKDSKEKTTTSRRYTKQKRTC 579
KK ++EKT + RR+ QKR C
Sbjct: 493 KKHTEEKTNSERRFMFQKRRC 513
>At2g47860.1 68415.m05973 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 635
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1
Query: 517 KKDSKEKTTTSRRYTKQKRTC 579
KK ++EKT + RR+ QKR C
Sbjct: 611 KKHTEEKTNSERRFMFQKRRC 631
>At4g36570.1 68417.m05192 myb family transcription factor conatins
PFAM profile: PF00249 myb-like DNA binding domain
Length = 85
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +1
Query: 25 KKTKLFILRSATIMSNNGAPDSWENEAEIIGEKGAKDS 138
K+ KLF AT + PD W N A +G K A+++
Sbjct: 15 KENKLFERALATY--DQDTPDRWHNVARAVGGKSAEEN 50
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/57 (29%), Positives = 29/57 (50%)
Frame = +2
Query: 509 ELTKKIPKKKPPRVEDTRSKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEK 679
+L+K + P + S++ +N+ IGHV GKSTI + + + K LE+
Sbjct: 11 DLSKLDVAQLHPLSPEVISRQATINIGTIGHVAHGKSTIVKAVSGVHTVKFKSELER 67
>At1g66880.1 68414.m07601 serine/threonine protein kinase family
protein contains protein kinase domain, Pfam:PF00069;
contains serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 1296
Score = 27.5 bits (58), Expect = 8.8
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +3
Query: 387 SVPPDVSPTADSWEVEADDA 446
SVPP +SP D W +D A
Sbjct: 1274 SVPPPISPETDKWTSSSDTA 1293
>At1g33770.1 68414.m04174 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 614
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/54 (29%), Positives = 27/54 (50%)
Frame = +1
Query: 121 KGAKDSNDVSSKISTLNVNAMEFVPSFSKPSQASDSTDSPTSPQNQKAVLRIQP 282
+G +DS I++ N N PSF K + +D T+S P ++ V ++P
Sbjct: 473 RGLRDSKVTPEFIASGNSNVSLTTPSFKKEKRFTD-TNSVIHPSSRSNVGEVKP 525
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = +2
Query: 542 PRVEDTRSKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEK 679
P + S++ +N+ IGHV GKST+ I + + K LE+
Sbjct: 22 PLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELER 67
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,830,790
Number of Sequences: 28952
Number of extensions: 207971
Number of successful extensions: 1073
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 916
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1067
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -