Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0537
(739 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE... 75 5e-14
At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat... 71 6e-13
At1g02010.1 68414.m00119 cytokinesis-related Sec1 protein, putat... 66 2e-11
At2g17980.1 68415.m02090 sec1 family protein similar to SWISS-PR... 39 0.004
At5g26940.4 68418.m03215 exonuclease family protein contains exo... 30 1.4
At5g26940.3 68418.m03214 exonuclease family protein contains exo... 30 1.4
At5g26940.2 68418.m03213 exonuclease family protein contains exo... 30 1.4
At5g26940.1 68418.m03212 exonuclease family protein contains exo... 30 1.4
At1g32440.1 68414.m04004 pyruvate kinase, putative similar to py... 29 3.2
At5g55550.3 68418.m06922 RNA recognition motif (RRM)-containing ... 27 9.8
At5g55550.2 68418.m06921 RNA recognition motif (RRM)-containing ... 27 9.8
At5g47180.2 68418.m05818 vesicle-associated membrane family prot... 27 9.8
At5g47180.1 68418.m05817 vesicle-associated membrane family prot... 27 9.8
>At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE)
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family
Length = 666
Score = 74.9 bits (176), Expect = 5e-14
Identities = 30/64 (46%), Positives = 53/64 (82%)
Frame = +1
Query: 70 SARIEWRVLIVDQLSMRMVSACCKMHDISAEGITLVEDIHKKREPLCTMDGIYLITPSEK 249
S++ W+VLI+D+L+++++S CKM DI+ EG++LVEDI ++R+PL +MD IY I P+++
Sbjct: 38 SSKSTWKVLIMDKLTVKIMSYACKMADITQEGVSLVEDIFRRRQPLPSMDAIYFIQPTKE 97
Query: 250 SVML 261
+V++
Sbjct: 98 NVIM 101
Score = 30.3 bits (65), Expect = 1.4
Identities = 19/44 (43%), Positives = 26/44 (59%), Gaps = 4/44 (9%)
Frame = +2
Query: 515 IAEQIATLCATLGEYPSVRYRS----DWERNVELAQLIQQKLDA 634
+A +IAT+ A+L E+P+VRYR+ D L LI KL A
Sbjct: 188 MASRIATVFASLREFPAVRYRAAKSLDASTMTTLRDLIPTKLAA 231
>At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family
Length = 662
Score = 71.3 bits (167), Expect = 6e-13
Identities = 30/84 (35%), Positives = 61/84 (72%)
Frame = +1
Query: 4 KALVGQKIMNEVIRYRGPKGKESARIEWRVLIVDQLSMRMVSACCKMHDISAEGITLVED 183
+ + ++++ E++R P+ S+++ W+VL++D+ +++++S+ CKM +I+ EGI+LVE
Sbjct: 19 RQITRERLLCEMLR---PERNGSSKLTWKVLVMDKFTVKIMSSACKMSEITQEGISLVEV 75
Query: 184 IHKKREPLCTMDGIYLITPSEKSV 255
I K R+P+ M+ IY I P+E++V
Sbjct: 76 ITKHRQPMTAMEVIYFIQPTEENV 99
Score = 31.1 bits (67), Expect = 0.80
Identities = 13/21 (61%), Positives = 18/21 (85%)
Frame = +2
Query: 515 IAEQIATLCATLGEYPSVRYR 577
+A++IAT+ A+L EYP VRYR
Sbjct: 188 VAKRIATVLASLKEYPFVRYR 208
Score = 29.9 bits (64), Expect = 1.8
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = +1
Query: 631 CL*GRRNQLWGEGPEKARSQLLVLDRGFDCVSPLLH 738
CL R Q + P+ +LL+LDR D ++PL+H
Sbjct: 236 CL-ARYKQTIEDFPQTETCELLILDRSIDQIAPLIH 270
>At1g02010.1 68414.m00119 cytokinesis-related Sec1 protein, putative
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family; non-consensus GC
donor splice site at exon boundary 46833
Length = 676
Score = 66.5 bits (155), Expect = 2e-11
Identities = 26/55 (47%), Positives = 45/55 (81%)
Frame = +1
Query: 85 WRVLIVDQLSMRMVSACCKMHDISAEGITLVEDIHKKREPLCTMDGIYLITPSEK 249
W++LI+D+++++++S CKM DI+ +GI+LVE++ K+REP+ MD IY I PS++
Sbjct: 45 WKILIMDRVTVKVMSQSCKMADITDQGISLVEELFKRREPMPGMDAIYFIQPSKE 99
>At2g17980.1 68415.m02090 sec1 family protein similar to
SWISS-PROT:P22213 SLY1 protein [Saccharomyces
cerevisiae]; contains Pfam domain, PF00995: Sec1 family
Length = 627
Score = 38.7 bits (86), Expect = 0.004
Identities = 17/67 (25%), Positives = 36/67 (53%)
Frame = +1
Query: 55 PKGKESARIEWRVLIVDQLSMRMVSACCKMHDISAEGITLVEDIHKKREPLCTMDGIYLI 234
P G + + +++LI D+ ++S + D+ G+TL I K R+P+ + +Y +
Sbjct: 25 PSGTANEEV-YKILIYDRFCQNILSPLTHVKDLRKHGVTLFFLIDKDRQPVHDVPAVYFV 83
Query: 235 TPSEKSV 255
P+E ++
Sbjct: 84 QPTESNL 90
>At5g26940.4 68418.m03215 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 316
Score = 30.3 bits (65), Expect = 1.4
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = +3
Query: 456 TFQCMYNPALAQTRNANMNVLLNK--SRPSVPRSANILP 566
TFQ + NP + NA+++ + N RP VPR ++P
Sbjct: 143 TFQTLVNPGVVPITNAHIHGIRNDMVCRPEVPRMEELIP 181
>At5g26940.3 68418.m03214 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 316
Score = 30.3 bits (65), Expect = 1.4
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = +3
Query: 456 TFQCMYNPALAQTRNANMNVLLNK--SRPSVPRSANILP 566
TFQ + NP + NA+++ + N RP VPR ++P
Sbjct: 143 TFQTLVNPGVVPITNAHIHGIRNDMVCRPEVPRMEELIP 181
>At5g26940.2 68418.m03213 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 316
Score = 30.3 bits (65), Expect = 1.4
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = +3
Query: 456 TFQCMYNPALAQTRNANMNVLLNK--SRPSVPRSANILP 566
TFQ + NP + NA+++ + N RP VPR ++P
Sbjct: 143 TFQTLVNPGVVPITNAHIHGIRNDMVCRPEVPRMEELIP 181
>At5g26940.1 68418.m03212 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 316
Score = 30.3 bits (65), Expect = 1.4
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = +3
Query: 456 TFQCMYNPALAQTRNANMNVLLNK--SRPSVPRSANILP 566
TFQ + NP + NA+++ + N RP VPR ++P
Sbjct: 143 TFQTLVNPGVVPITNAHIHGIRNDMVCRPEVPRMEELIP 181
>At1g32440.1 68414.m04004 pyruvate kinase, putative similar to
pyruvate kinase isozyme G, chloroplast precursor
[Nicotiana tabacum] SWISS-PROT:Q40546
Length = 571
Score = 29.1 bits (62), Expect = 3.2
Identities = 18/78 (23%), Positives = 36/78 (46%)
Frame = +3
Query: 306 AHVFFTEACPDILFNELSRSQIAKYIKTLKEINIAFIPYEQQVFSLDSPETFQCMYNPAL 485
AH F +++ R++ + ++T A+ + Q+F+ + + +P +
Sbjct: 410 AHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTAYKGHMGQMFAFHASIMANTLSSPLI 469
Query: 486 AQTRNANMNVLLNKSRPS 539
TR +M VLL+ RPS
Sbjct: 470 VFTRTGSMAVLLSHYRPS 487
>At5g55550.3 68418.m06922 RNA recognition motif (RRM)-containing
protein similar to DAZ associated protein 1 [Homo
sapiens] GI:8671754; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 460
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = +3
Query: 438 SLDSPETFQCMYNPALAQTRNANMNVLLNKS 530
S + PET+ YN QT N + LNK+
Sbjct: 424 SANDPETYNGSYNVGNRQTNRGNKKIHLNKT 454
>At5g55550.2 68418.m06921 RNA recognition motif (RRM)-containing
protein similar to DAZ associated protein 1 [Homo
sapiens] GI:8671754; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 460
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = +3
Query: 438 SLDSPETFQCMYNPALAQTRNANMNVLLNKS 530
S + PET+ YN QT N + LNK+
Sbjct: 424 SANDPETYNGSYNVGNRQTNRGNKKIHLNKT 454
>At5g47180.2 68418.m05818 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia], to VAMP-associated protein
B GI:4240464 [Rattus norvegicus] and to
Vesicle-associated membrane protein/synaptobrevin
binding protein (VAP-33) (SP:Q16943)[Aplysia
californica]
Length = 220
Score = 27.5 bits (58), Expect = 9.8
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = -1
Query: 715 RIRGPEREAENGLFLVLRPIVGFVGLISIKFLL 617
R R +R + NGL L L +VG +GLI I F+L
Sbjct: 181 RRRRNQRNSGNGLSLKLAAMVGLIGLI-IGFIL 212
>At5g47180.1 68418.m05817 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia], to VAMP-associated protein
B GI:4240464 [Rattus norvegicus] and to
Vesicle-associated membrane protein/synaptobrevin
binding protein (VAP-33) (SP:Q16943)[Aplysia
californica]
Length = 220
Score = 27.5 bits (58), Expect = 9.8
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = -1
Query: 715 RIRGPEREAENGLFLVLRPIVGFVGLISIKFLL 617
R R +R + NGL L L +VG +GLI I F+L
Sbjct: 181 RRRRNQRNSGNGLSLKLAAMVGLIGLI-IGFIL 212
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,751,334
Number of Sequences: 28952
Number of extensions: 316964
Number of successful extensions: 851
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 820
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 851
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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