Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0531
(765 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g18810.1 68416.m02389 protein kinase family protein contains ... 36 0.030
At2g33850.1 68415.m04155 expressed protein contains 1 transmembr... 31 0.84
At3g20770.1 68416.m02627 ethylene-insensitive 3 (EIN3) identical... 31 1.1
At1g28400.1 68414.m03492 expressed protein similar to E6 (GI:100... 31 1.1
At5g12850.1 68418.m01475 zinc finger (CCCH-type) family protein ... 30 1.5
At1g75260.1 68414.m08743 isoflavone reductase family protein sim... 30 1.9
At1g79940.1 68414.m09342 DNAJ heat shock N-terminal domain-conta... 29 4.5
At1g78580.1 68414.m09158 alpha, alpha-trehalose-phosphate syntha... 29 4.5
At1g23000.1 68414.m02874 heavy-metal-associated domain-containin... 28 5.9
At1g10170.1 68414.m01147 NF-X1 type zinc finger family protein c... 28 5.9
At2g47810.1 68415.m05967 histone-like transcription factor (CBF/... 28 7.8
At1g62010.1 68414.m06994 mitochondrial transcription termination... 28 7.8
>At3g18810.1 68416.m02389 protein kinase family protein contains
Pfam PF00069: Protein kinase domain
Length = 700
Score = 35.9 bits (79), Expect = 0.030
Identities = 21/67 (31%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
Frame = +1
Query: 514 NNNDDTSNMNFGSQNSNHQS-THNTIRSDKNNPTVDNFFTNANGNRKKEELDLNQSYKNP 690
NNN+D +N N + N+N+ + +N ++ NN +N N NGN D N + N
Sbjct: 70 NNNNDGNNGNNNNDNNNNNNGNNNNDNNNGNNKDNNNNGNNNNGNNNNGN-DNNGNNNNG 128
Query: 691 SNYEKQN 711
+N + N
Sbjct: 129 NNNDNNN 135
Score = 32.3 bits (70), Expect = 0.36
Identities = 23/78 (29%), Positives = 33/78 (42%), Gaps = 1/78 (1%)
Frame = +1
Query: 514 NNNDDTSNMNFGSQNSN-HQSTHNTIRSDKNNPTVDNFFTNANGNRKKEELDLNQSYKNP 690
NNN+D +N N N+N + + N + NN +N N N N+ N+S P
Sbjct: 91 NNNNDNNNGNNKDNNNNGNNNNGNNNNGNDNNGNNNNGNNNDNNNQNNGGGSNNRSPPPP 150
Query: 691 SNYEKQNIQPIILVLANP 744
S +N LA P
Sbjct: 151 SRNSDRNSPSPPRALAPP 168
Score = 31.5 bits (68), Expect = 0.64
Identities = 18/71 (25%), Positives = 34/71 (47%), Gaps = 2/71 (2%)
Frame = +1
Query: 514 NNNDDTSNMNFGSQNSNHQSTHNTIRSDK--NNPTVDNFFTNANGNRKKEELDLNQSYKN 687
NNN+D +N N G+ N+++ + +N ++ NN +N + NGN + N + N
Sbjct: 79 NNNNDNNNNNNGNNNNDNNNGNNKDNNNNGNNNNGNNNNGNDNNGNNNNGNNNDNNNQNN 138
Query: 688 PSNYEKQNIQP 720
++ P
Sbjct: 139 GGGSNNRSPPP 149
Score = 29.5 bits (63), Expect = 2.6
Identities = 18/67 (26%), Positives = 28/67 (41%)
Frame = +1
Query: 511 PNNNDDTSNMNFGSQNSNHQSTHNTIRSDKNNPTVDNFFTNANGNRKKEELDLNQSYKNP 690
P ++D S + N+N + N + NN +N N NGN K + N + N
Sbjct: 56 PPSSDSQSPPSPQGNNNNDGNNGNNNNDNNNNNNGNNNNDNNNGNNKDNNNNGNNNNGNN 115
Query: 691 SNYEKQN 711
+N N
Sbjct: 116 NNGNDNN 122
>At2g33850.1 68415.m04155 expressed protein contains 1 transmembrane
domain; similar to Protein E6 (Swiss-Prot:Q01197)
[Gossypium hirsutum]
Length = 267
Score = 31.1 bits (67), Expect = 0.84
Identities = 20/82 (24%), Positives = 36/82 (43%), Gaps = 2/82 (2%)
Frame = +3
Query: 9 PEHRDPQDYTAL--NERKSQDKHTENLIPNLTNSHKSYGYTGIDKDESIVSQDKVAFTND 182
P+ ++P + + NE+K D IP N + YG+ D + ++ +K D
Sbjct: 39 PKEQNPNNLVPIETNEKKEPDDQNPAFIPQSENGYGLYGHETTDNNNEELNNNKY---ED 95
Query: 183 NGNLYQSKESRSENSGTSTQDT 248
N N S + S + TQ++
Sbjct: 96 NVNYDDSFSTPSLSETAQTQES 117
>At3g20770.1 68416.m02627 ethylene-insensitive 3 (EIN3) identical to
ethylene-insensitive3 GI:2224933 from [Arabidopsis
thaliana]
Length = 628
Score = 30.7 bits (66), Expect = 1.1
Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = +1
Query: 514 NNNDDTSNMNFGSQNSNHQSTHNTIRSDKNN-PTVDNFFTNANGNR 648
NNN+ ++N F N+N+ + +D NN N N++GNR
Sbjct: 539 NNNNSSNNQTFFQGNNNNNNVFKFDTADHNNFEAAHNNNNNSSGNR 584
>At1g28400.1 68414.m03492 expressed protein similar to E6
(GI:1000090) [Gossypium barbadense]
Length = 335
Score = 30.7 bits (66), Expect = 1.1
Identities = 19/78 (24%), Positives = 31/78 (39%)
Frame = +1
Query: 514 NNNDDTSNMNFGSQNSNHQSTHNTIRSDKNNPTVDNFFTNANGNRKKEELDLNQSYKNPS 693
NNN++ + NF + +N++ N + + NN +N + KEE + N
Sbjct: 153 NNNNNKYDANFKEEFNNNKYDENYAKEEFNNNNNNNNYNYKYDENVKEESFPENNEDNKK 212
Query: 694 NYEKQNIQPIILVLANPY 747
N N L PY
Sbjct: 213 NVYNSNAYGTELERETPY 230
>At5g12850.1 68418.m01475 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar) and Pfam domain,
PF00023: Ankyrin repeat
Length = 706
Score = 30.3 bits (65), Expect = 1.5
Identities = 14/47 (29%), Positives = 27/47 (57%)
Frame = +2
Query: 305 VAGSSGDSIIMSRAQTNKGPLYYIVKSNNFNAANNLEQSTILPQTSA 445
V S S ++++ Q NK + +K+N ++ N+EQ ++L Q S+
Sbjct: 514 VLSPSHKSALLNQLQNNKQSMLSPIKTNLMSSPKNVEQHSLLQQASS 560
>At1g75260.1 68414.m08743 isoflavone reductase family protein
similar to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: Isoflavone reductase
Length = 593
Score = 29.9 bits (64), Expect = 1.9
Identities = 15/50 (30%), Positives = 24/50 (48%)
Frame = +1
Query: 511 PNNNDDTSNMNFGSQNSNHQSTHNTIRSDKNNPTVDNFFTNANGNRKKEE 660
P +T N + NHQ T + S+ NP V N +++ +RK +E
Sbjct: 273 PEKKTETQNRTEIPTDDNHQKTKGALTSNLGNPRVTNREGSSSMSRKIKE 322
>At1g79940.1 68414.m09342 DNAJ heat shock N-terminal
domain-containing protein / sec63 domain-containing
protein similar to SP|Q9UGP8 Translocation protein SEC63
homolog {Homo sapiens}; contains Pfam profiles PF00226
DnaJ domain, PF02889 Sec63 domain
Length = 702
Score = 28.7 bits (61), Expect = 4.5
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +3
Query: 132 DKDESIVSQDKVAFTNDNGNLYQSKESRSENSGTSTQ 242
D+DE +K NG++ Q KES SE SG+ +
Sbjct: 666 DEDEKKDMDEKRGSKKANGSVKQKKESSSEESGSEEE 702
>At1g78580.1 68414.m09158 alpha, alpha-trehalose-phosphate synthase,
UDP-forming, putative / trehalose-6-phosphate synthase,
putative / UDP-glucose-glucosephosphate
glucosyltransferase, putative similar to
trehalose-6-phosphate synthase SL-TPS/P [Selaginella
lepidophylla] GI:4100325; contains Pfam profiles PF00982:
Glycosyltransferase family 20, PF02358:
Trehalose-phosphatase
Length = 942
Score = 28.7 bits (61), Expect = 4.5
Identities = 16/65 (24%), Positives = 30/65 (46%)
Frame = +1
Query: 517 NNDDTSNMNFGSQNSNHQSTHNTIRSDKNNPTVDNFFTNANGNRKKEELDLNQSYKNPSN 696
++D + + G + +STHN +S + + +N+N N K + L K+ SN
Sbjct: 825 SSDSGAKSSSGDRRPPSKSTHNNNKSGSKSSSS----SNSNNNNKSSQRSLQSERKSGSN 880
Query: 697 YEKQN 711
+ N
Sbjct: 881 HSLGN 885
>At1g23000.1 68414.m02874 heavy-metal-associated domain-containing
protein similar to farnesylated protein ATFP3
[GI:4097547]; contains PF00403 Heavy-metal-associated
domain
Length = 358
Score = 28.3 bits (60), Expect = 5.9
Identities = 17/66 (25%), Positives = 27/66 (40%)
Frame = +1
Query: 511 PNNNDDTSNMNFGSQNSNHQSTHNTIRSDKNNPTVDNFFTNANGNRKKEELDLNQSYKNP 690
P ND N+N Q + KN P F +G+ +EE D + + P
Sbjct: 89 PKTNDFIKNVNQKGQKQGSAKSGIEACKPKNGPKGAAFVAEEDGDGSEEE-DGDVQFPKP 147
Query: 691 SNYEKQ 708
+N ++Q
Sbjct: 148 ANQQQQ 153
>At1g10170.1 68414.m01147 NF-X1 type zinc finger family protein
contains Pfam PF01422: NF-X1 type zinc finger; similar
to transcriptional repressor NF-X1 (SP:Q12986) [Homo
sapiens]; similar to EST gb|T21002
Length = 1188
Score = 28.3 bits (60), Expect = 5.9
Identities = 15/69 (21%), Positives = 28/69 (40%)
Frame = +3
Query: 24 PQDYTALNERKSQDKHTENLIPNLTNSHKSYGYTGIDKDESIVSQDKVAFTNDNGNLYQS 203
P + N +Q + ++N+ P N H+ Y S S + + N ++
Sbjct: 88 PNQHRRYNAPDNQHQRSDNIGPPQPNQHRRYNAPDNQHQRSDNSGPPQPYRHRRNNAPEN 147
Query: 204 KESRSENSG 230
+ RS+N G
Sbjct: 148 QHQRSDNIG 156
>At2g47810.1 68415.m05967 histone-like transcription factor
(CBF/NF-Y) family protein contains Pfam PF00808 :
Histone-like transcription factor (CBF/NF-Y) and
archaeal histone; similar to LEC1-like protein
(GI:22536010) [Phaseolus coccineus]
Length = 160
Score = 27.9 bits (59), Expect = 7.8
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +1
Query: 508 FPNNNDDTSNMNFGSQNSNHQSTHN 582
F N N NFGS +SNHQ H+
Sbjct: 8 FQNPIPRYQNYNFGSSSSNHQHEHD 32
>At1g62010.1 68414.m06994 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 415
Score = 27.9 bits (59), Expect = 7.8
Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 5/61 (8%)
Frame = +3
Query: 108 KSYGYTGIDKDESIVSQDKVAFTNDN---GNLYQSKESRSENSGTSTQ--DTVPKLFMYR 272
+SYG+T I S +V ND G+ Q +SR +S T+ TVPK+ R
Sbjct: 78 RSYGFTDSQISSIIRSDSRVLIDNDATSLGSKLQFLQSRGASSSELTEVVSTVPKILGKR 137
Query: 273 Q 275
+
Sbjct: 138 E 138
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,946,696
Number of Sequences: 28952
Number of extensions: 295975
Number of successful extensions: 970
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 887
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 934
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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