BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0503 (725 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4.02c |||conserved fungal protein|Schizosaccharomyces pombe|... 27 2.1 SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor Raf2|S... 25 8.3 SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc... 25 8.3 >SPBC4.02c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 456 Score = 27.5 bits (58), Expect = 2.1 Identities = 15/54 (27%), Positives = 26/54 (48%) Frame = +1 Query: 103 KNSFAQLSLYNSTESETIANTTTKKDEDGPSDDTSMIEVPPNTAGCQEQGTIWI 264 +N+F + + ES + AN++ + D S E PP+T+ G +WI Sbjct: 285 RNAFFNIPIAEIMESVS-ANSSRRNSHDSNSTIICEPEYPPDTSFNSSPGRLWI 337 >SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor Raf2|Schizosaccharomyces pombe|chr 3|||Manual Length = 636 Score = 25.4 bits (53), Expect = 8.3 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = -1 Query: 443 KLFAEIKHYIALRTNTRQRYSQ 378 KLF +IKH I R++ +Q SQ Sbjct: 352 KLFNDIKHAIGFRSSVQQARSQ 373 >SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 25.4 bits (53), Expect = 8.3 Identities = 12/32 (37%), Positives = 14/32 (43%) Frame = +3 Query: 3 CAQCGLKFIYLCFWSTYFDPGYSSNIWQSRPR 98 C Q F YLCFW SSN W + + Sbjct: 156 CEQGYSFFYYLCFWFLGLYIYRSSNYWSNEEK 187 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,085,612 Number of Sequences: 5004 Number of extensions: 67211 Number of successful extensions: 183 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 177 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 183 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 341222980 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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