Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0481
(785 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 63 2e-10
At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 56 4e-08
At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 53 2e-07
At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 52 6e-07
At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 49 4e-06
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 48 9e-06
At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 45 5e-05
At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 45 7e-05
At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 45 7e-05
At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4) ... 30 2.0
At4g22880.1 68417.m03304 leucoanthocyanidin dioxygenase, putativ... 30 2.0
At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3) ... 29 3.5
At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /... 29 4.6
At3g16890.1 68416.m02159 pentatricopeptide (PPR) repeat-containi... 28 6.1
At3g07140.2 68416.m00851 GPI transamidase component Gpi16 subuni... 28 6.1
At3g07140.1 68416.m00850 GPI transamidase component Gpi16 subuni... 28 6.1
At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative... 28 6.1
At1g47770.1 68414.m05313 hypothetical protein 28 8.1
>At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to
delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from
[Arabidopsis thaliana]
Length = 307
Score = 63.3 bits (147), Expect = 2e-10
Identities = 36/109 (33%), Positives = 50/109 (45%)
Frame = +3
Query: 240 KCDSVFILHVGGVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLP 419
K + F +H + + F + A+W T +F Y GLGIT HR AH+S+K
Sbjct: 43 KFSASFTVHSLALLAPFYFTWSALWVTFLF----YTIGGLGITVSYHRNLAHRSFKVPKW 98
Query: 420 LRILLTIFNTIAFQDAVVDWARDHRMHHKYQRPMRTPTTQPEDSSFPTL 566
L LL +A Q +DW HR HH++ R P + E F L
Sbjct: 99 LEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHL 147
Score = 38.7 bits (86), Expect = 0.004
Identities = 18/55 (32%), Positives = 30/55 (54%)
Frame = +2
Query: 503 QISETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 667
Q ++++ DPH+ GF+FSH+ W+ + K G +V DL+ RF +K
Sbjct: 127 QFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC-GRRANVEDLKRQWFYRFLQK 180
>At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to
delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180
GI:2970034 from [Arabidopsis thaliana]
Length = 305
Score = 55.6 bits (128), Expect = 4e-08
Identities = 29/89 (32%), Positives = 38/89 (42%)
Frame = +3
Query: 300 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 479
F W A+ +Y GLGIT HR AH+S+K L +A Q +DW
Sbjct: 57 FNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDW 116
Query: 480 ARDHRMHHKYQRPMRTPTTQPEDSSFPTL 566
HR HH++ R P + E F L
Sbjct: 117 VSTHRYHHQFTDSDRDPHSPNEGFWFSHL 145
Score = 38.7 bits (86), Expect = 0.004
Identities = 20/62 (32%), Positives = 34/62 (54%), Gaps = 2/62 (3%)
Frame = +2
Query: 503 QISETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK--YYQ 676
Q +++D DPH+ GF+FSH+ WL + ++ G +V DL+ +F ++ Y
Sbjct: 125 QFTDSDRDPHSPNEGFWFSHLLWLFDTGY-LVEKCGRRTNVEDLKRQWYYKFLQRTVLYH 183
Query: 677 IL 682
IL
Sbjct: 184 IL 185
>At1g06360.1 68414.m00672 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 52.8 bits (121), Expect = 2e-07
Identities = 30/93 (32%), Positives = 38/93 (40%)
Frame = +3
Query: 300 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 479
F W F LY + L IT HR AH+S+K L L F A Q +DW
Sbjct: 51 FNYKWEALRFGFVLYALTSLSITFSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDW 110
Query: 480 ARDHRMHHKYQRPMRTPTTQPEDSSFPTLAGCC 578
HR HH++ R P + E F + C
Sbjct: 111 VSIHRFHHQFTDSDRDPHSPIEGFWFSHVWWIC 143
Score = 39.1 bits (87), Expect = 0.003
Identities = 16/45 (35%), Positives = 28/45 (62%)
Frame = +2
Query: 503 QISETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLR 637
Q +++D DPH+ GF+FSH+ W+ ++ + K G +V DL+
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVWWICDTRYIKYKCGGRN-NVMDLK 162
>At3g15850.1 68416.m02005 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 371
Score = 51.6 bits (118), Expect = 6e-07
Identities = 28/78 (35%), Positives = 37/78 (47%), Gaps = 1/78 (1%)
Frame = +3
Query: 300 FKAMWRTSIFAIFLYLCSGL-GITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVD 476
F+ WR A LY+ +GL GIT HR +HK++K L L A Q +D
Sbjct: 121 FQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPID 180
Query: 477 WARDHRMHHKYQRPMRTP 530
W HR HH++ R P
Sbjct: 181 WVSTHRYHHQFCDSDRDP 198
Score = 46.4 bits (105), Expect = 2e-05
Identities = 31/98 (31%), Positives = 47/98 (47%), Gaps = 4/98 (4%)
Frame = +2
Query: 503 QISETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQIL 682
Q ++D DPH+ GF+FSH+ W + + + G +V DL P RF + Y IL
Sbjct: 190 QFCDSDRDPHSPLDGFWFSHMNW-MFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTY-IL 247
Query: 683 MPL----ACFIMPTYVPTLWGETVWNSFYVWQFSATYM 784
PL A + M + +WG V VW + T++
Sbjct: 248 HPLALAVALYAMGGFPFIVWGMGV---RIVWVYHITWL 282
>At3g15870.1 68416.m02007 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 292
Score = 48.8 bits (111), Expect = 4e-06
Identities = 27/96 (28%), Positives = 45/96 (46%)
Frame = +3
Query: 282 GGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQ 461
G L +A W +F +++ +G+ IT HR +H+S+ L L +AFQ
Sbjct: 43 GSILLQLEAFW---VFPWLVFI-NGICITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQ 98
Query: 462 DAVVDWARDHRMHHKYQRPMRTPTTQPEDSSFPTLA 569
++W +HR HHK+ R P + + F +A
Sbjct: 99 GDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMA 134
Score = 41.5 bits (93), Expect = 6e-04
Identities = 21/64 (32%), Positives = 34/64 (53%)
Frame = +2
Query: 512 ETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQILMPL 691
ET DPH+ T+GF+FSH+ W + ++ G +V+DL P RF ++ + M
Sbjct: 116 ETQRDPHSPTQGFWFSHMAW-IFDTSSILENCGGEENVDDLVRQPFYRFLQRTVLLHMMA 174
Query: 692 ACFI 703
F+
Sbjct: 175 YSFL 178
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 47.6 bits (108), Expect = 9e-06
Identities = 24/77 (31%), Positives = 31/77 (40%)
Frame = +3
Query: 300 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 479
F W F + L + + L IT HR HKS+K L A Q +DW
Sbjct: 51 FNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDW 110
Query: 480 ARDHRMHHKYQRPMRTP 530
HR HH++ R P
Sbjct: 111 VSTHRFHHQFTDSDRDP 127
Score = 39.1 bits (87), Expect = 0.003
Identities = 18/52 (34%), Positives = 28/52 (53%)
Frame = +2
Query: 503 QISETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 658
Q +++D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At1g06350.1 68414.m00671 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 300
Score = 45.2 bits (102), Expect = 5e-05
Identities = 25/86 (29%), Positives = 35/86 (40%)
Frame = +3
Query: 300 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 479
F W F + L+ + L IT HR +H+S+K L A Q +DW
Sbjct: 52 FNFKWEALRFGLVLFALTTLSITFSFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDW 111
Query: 480 ARDHRMHHKYQRPMRTPTTQPEDSSF 557
HR HH++ R P + E F
Sbjct: 112 VSIHRFHHQFTDSDRDPHSPKEGLLF 137
Score = 37.1 bits (82), Expect = 0.013
Identities = 17/55 (30%), Positives = 30/55 (54%)
Frame = +2
Query: 503 QISETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 667
Q +++D DPH+ G FSHI W+ ++ + K G +V DL+ +F ++
Sbjct: 120 QFTDSDRDPHSPKEGLLFSHILWIFDTQYIKYKCGGRD-NVLDLKKQWFYKFLRR 173
>At1g06120.1 68414.m00641 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported
by cDNA:gi_12083275_gb_AF332434.1_AF332434
Length = 299
Score = 44.8 bits (101), Expect = 7e-05
Identities = 23/77 (29%), Positives = 31/77 (40%)
Frame = +3
Query: 300 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 479
F W FA + + + L IT HR H+S+K L A Q +DW
Sbjct: 51 FNYTWEAFRFAAMVGISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDW 110
Query: 480 ARDHRMHHKYQRPMRTP 530
HR HH++ R P
Sbjct: 111 VSTHRFHHQFTDSDRDP 127
Score = 39.1 bits (87), Expect = 0.003
Identities = 18/52 (34%), Positives = 28/52 (53%)
Frame = +2
Query: 503 QISETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 658
Q +++D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At1g06100.1 68414.m00639 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 44.8 bits (101), Expect = 7e-05
Identities = 24/77 (31%), Positives = 32/77 (41%)
Frame = +3
Query: 300 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 479
F W F I L + + L IT HR H+S+K L +A Q +DW
Sbjct: 51 FNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDW 110
Query: 480 ARDHRMHHKYQRPMRTP 530
HR HH++ R P
Sbjct: 111 VSIHRFHHQFTDSDRDP 127
Score = 41.5 bits (93), Expect = 6e-04
Identities = 25/77 (32%), Positives = 39/77 (50%)
Frame = +2
Query: 503 QISETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQIL 682
Q +++D DPH+ GF+FSH+ W+ + K G +V DL+ RF KK ++
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVLWIFDTDYIREKC-GRRNNVMDLKQQWFYRFLKK--TLV 175
Query: 683 MPLACFIMPTYVPTLWG 733
+ + F Y LWG
Sbjct: 176 LHILAFWTLIY---LWG 189
>At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4)
identical to metal transporter Nramp4 [Arabidopsis
thaliana] gi|6468014|gb|AAF13279; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 512
Score = 29.9 bits (64), Expect = 2.0
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +3
Query: 273 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 380
G GY ++ MW T+I + L + LG+ G H
Sbjct: 79 GAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRH 114
>At4g22880.1 68417.m03304 leucoanthocyanidin dioxygenase, putative /
anthocyanidin synthase, putative similar to SP|P51091
[Malus domestica]; contains PF03171 2OG-Fe(II) oxygenase
superfamily
Length = 356
Score = 29.9 bits (64), Expect = 2.0
Identities = 20/85 (23%), Positives = 35/85 (41%), Gaps = 1/85 (1%)
Frame = -2
Query: 355 PEHKYKNMANIEVLHMALKRNKYPP*TPPTCRIKTESHFSTQSSKVSSLLRE-VHGAVHL 179
P+ K + +E L + +K N YP P + E+H T S ++ +L V G
Sbjct: 195 PDRLEKEVGGLEELLLQMKINYYPKCPQPELALGVEAH--TDVSALTFILHNMVPGLQLF 252
Query: 178 LAGYWRRLRTIPTKPPVSFGEAFSL 104
G W + +P + G+ +
Sbjct: 253 YEGKWVTAKCVPDSIVMHIGDTLEI 277
>At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3)
identical to metal transporter Nramp3 [Arabidopsis
thaliana] gi|6468012|gb|AAF13278; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 509
Score = 29.1 bits (62), Expect = 3.5
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +3
Query: 273 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 380
G GY L+ MW T++ + L + LG+ G H
Sbjct: 83 GAVAGYSLLWLLMWATAMGLLVQLLSARLGVATGRH 118
>At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Lycopersicon esculentum] GI:7381227;
contains PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 468
Score = 28.7 bits (61), Expect = 4.6
Identities = 16/58 (27%), Positives = 24/58 (41%)
Frame = +1
Query: 139 SWVLYEADANNLPEDAPPHVPPSAEKRPWKIVWRNVILFLFCMSVVFMEGTCSFSKPC 312
S+ ++ D +P D P P S KR W +R + L V+ G + PC
Sbjct: 124 SYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPC 181
>At3g16890.1 68416.m02159 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 659
Score = 28.3 bits (60), Expect = 6.1
Identities = 21/81 (25%), Positives = 34/81 (41%), Gaps = 1/81 (1%)
Frame = +3
Query: 93 VHCFSENASPKETGGFVGIVRSRRQ*P-ARRCTAPCTSLSREETLEDCVEKCDSVFILHV 269
++C S N+ KETG F+ + R P + A + L + L + D V
Sbjct: 327 LYCLSNNSMAKETGQFLRKIGERGYIPDSSTFNAAMSCLLKGHDLVETCRIFDGFVSRGV 386
Query: 270 GGVYGGYLFLFKAMWRTSIFA 332
+ GYL L +A+ F+
Sbjct: 387 KPGFNGYLVLVQALLNAQRFS 407
>At3g07140.2 68416.m00851 GPI transamidase component Gpi16 subunit
family protein similar to phosphatidyl inositol glycan
class T (GI:14456615) [Homo sapiens]; contains Pfam
profile PF04113: Gpi16 subunit, GPI transamidase
component
Length = 643
Score = 28.3 bits (60), Expect = 6.1
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +1
Query: 196 VPPSAEKRPWKIVWRNVILFLFCMSVVFMEGTCSFSKPCGG 318
VP S WK + + + LFC S+ F+E + S++ P G
Sbjct: 122 VPQSQVDTSWKNL-THALSGLFCASINFLESSTSYAAPTWG 161
>At3g07140.1 68416.m00850 GPI transamidase component Gpi16 subunit
family protein similar to phosphatidyl inositol glycan
class T (GI:14456615) [Homo sapiens]; contains Pfam
profile PF04113: Gpi16 subunit, GPI transamidase
component
Length = 644
Score = 28.3 bits (60), Expect = 6.1
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +1
Query: 196 VPPSAEKRPWKIVWRNVILFLFCMSVVFMEGTCSFSKPCGG 318
VP S WK + + + LFC S+ F+E + S++ P G
Sbjct: 122 VPQSQVDTSWKNL-THALSGLFCASINFLESSTSYAAPTWG 161
>At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative
(NRAMP2) similar to metal transporter Nramp3
[Arabidopsis thaliana] gi|6468012|gb|AAF13278; member of
the natural resistance-associated macrophage protein
(NRAMP) metal transporter family, PMID:11500563
Length = 530
Score = 28.3 bits (60), Expect = 6.1
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +3
Query: 273 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 380
G GY L+ MW T++ + L + +G+ G H
Sbjct: 95 GAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRH 130
>At1g47770.1 68414.m05313 hypothetical protein
Length = 205
Score = 27.9 bits (59), Expect = 8.1
Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 2/36 (5%)
Frame = +3
Query: 243 CDSVF--ILHVGGVYGGYLFLFKAMWRTSIFAIFLY 344
CD F + H+GG YGG + L ++FAI +Y
Sbjct: 139 CDGGFNKMAHLGGYYGGEIVLHLFKGSYALFAIAVY 174
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,969,600
Number of Sequences: 28952
Number of extensions: 487206
Number of successful extensions: 1358
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1286
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1357
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1765546400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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