Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0475
(594 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g10890.1 68414.m01251 F-box family protein contains Pfam PF00... 29 1.8
At2g15550.1 68415.m01781 hypothetical protein similar to zinc fi... 29 2.3
At4g20900.1 68417.m03030 male sterility MS5 / pollenless 3 nearl... 28 4.1
At2g16410.1 68415.m01879 hypothetical protein similar to zinc fi... 28 4.1
At1g73480.1 68414.m08507 hydrolase, alpha/beta fold family prote... 28 4.1
At5g02540.1 68418.m00188 short-chain dehydrogenase/reductase (SD... 28 5.4
At1g47510.1 68414.m05273 endonuclease/exonuclease/phosphatase fa... 28 5.4
At4g29750.1 68417.m04237 expressed protein contains Pfam domain,... 27 7.1
At2g44020.1 68415.m05473 mitochondrial transcription termination... 27 7.1
At5g03060.1 68418.m00254 expressed protein ; expression supporte... 27 9.4
At4g27500.1 68417.m03950 expressed protein non-consensus GA dono... 27 9.4
At3g26500.1 68416.m03305 leucine-rich repeat family protein 27 9.4
At3g15115.1 68416.m01912 expressed protein 27 9.4
At2g25460.1 68415.m03049 expressed protein 27 9.4
At2g22360.1 68415.m02653 DNAJ heat shock family protein similar ... 27 9.4
At2g16090.1 68415.m01845 zinc finger protein-related contains si... 27 9.4
At1g76600.1 68414.m08913 expressed protein 27 9.4
>At1g10890.1 68414.m01251 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 592
Score = 29.5 bits (63), Expect = 1.8
Identities = 18/62 (29%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
Frame = +1
Query: 256 IKDPKSKAEISTLLREGQRNIHPSV-KKLLGKKEVDISEIFKSRAPRNKSKKDYSAMLQK 432
++ K K EI TLL EG++ ++ V +L +KE + E K + R + +K+ + +
Sbjct: 102 LQSEKIKMEILTLLEEGRKRLNEEVAAQLEEEKEASLIEA-KEKEEREQQEKEERERIAE 160
Query: 433 RN 438
N
Sbjct: 161 EN 162
>At2g15550.1 68415.m01781 hypothetical protein similar to zinc
finger protein [Arabidopsis thaliana] GI:976277
Length = 589
Score = 29.1 bits (62), Expect = 2.3
Identities = 20/53 (37%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = -3
Query: 568 RVSTVALGFRARGIFFGLLV-LQRVQF*SPCWGGASSWSLPRWCSYVSAAWPN 413
RV V+LG FF V LQ+V PC S++L RW +V ++PN
Sbjct: 79 RVRGVSLGDARFQFFFESEVDLQKVLNKRPCHFNKWSFALERWEPHVGTSFPN 131
>At4g20900.1 68417.m03030 male sterility MS5 / pollenless 3 nearly
identical to male sterility MS5 [Arabidopsis thaliana]
GI:3859112, pollenless3 [Arabidopsis thaliana]
GI:4028970
Length = 450
Score = 28.3 bits (60), Expect = 4.1
Identities = 18/65 (27%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
Frame = +1
Query: 346 KKEVDISEIFKSRAPR-NKSKKDYSAMLQKRNYTISADSTKTLRPNME-IKTEPAAGRVD 519
K E D+SE F + N+ K++ + +NY+ + S ++RPN + T+P R+
Sbjct: 298 KPEADLSEKFYAGCSFVNRMKENIAPGTANKNYSDVSSSPASVRPNSAGLYTQPRRCRLF 357
Query: 520 QRRSR 534
+ +R
Sbjct: 358 EEETR 362
>At2g16410.1 68415.m01879 hypothetical protein similar to zinc
finger protein [Arabidopsis thaliana] GI:976277
Length = 204
Score = 28.3 bits (60), Expect = 4.1
Identities = 18/56 (32%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
Frame = -3
Query: 568 RVSTVALGFRARGIFFGLLV-LQRVQF*SPCWGGASSWSLPRWCSYVSAAWPNNLS 404
RV V+LG FF LQ+V PC S++L RW S++ ++P+ ++
Sbjct: 77 RVRGVSLGDSRFQFFFESETDLQKVLNKRPCHFSKWSFALERWKSHIGISFPDTMT 132
>At1g73480.1 68414.m08507 hydrolase, alpha/beta fold family protein
low similarity to monoglyceride lipase from [Homo
sapiens] GI:14594904, [Mus musculus] GI:2632162;
contains Pfam profile PF00561: hydrolase, alpha/beta
fold family
Length = 463
Score = 28.3 bits (60), Expect = 4.1
Identities = 13/45 (28%), Positives = 21/45 (46%)
Frame = +1
Query: 346 KKEVDISEIFKSRAPRNKSKKDYSAMLQKRNYTISADSTKTLRPN 480
++E+ I + + S +DYS KR T+ + S L PN
Sbjct: 165 RRELAIKRVLEDEGGDGSSVRDYSLFTTKRGDTLFSQSWSPLSPN 209
>At5g02540.1 68418.m00188 short-chain dehydrogenase/reductase (SDR)
family protein contains INTERPRO family IPR002198
Short-chain dehydrogenase/reductase (SDR) superfamily
Length = 331
Score = 27.9 bits (59), Expect = 5.4
Identities = 19/57 (33%), Positives = 30/57 (52%), Gaps = 8/57 (14%)
Frame = +1
Query: 316 IHPSVKKLLGKKEVDISEIFKSRAPRNKSKK----DYSAML----QKRNYTISADST 462
+HPSVK + GK D +E+ S+ R+++ D+S L K+NY D+T
Sbjct: 275 LHPSVKGVTGKYFADCNEVTPSKLARDETLAQKLWDFSVKLINSVSKKNYLGFDDTT 331
>At1g47510.1 68414.m05273 endonuclease/exonuclease/phosphatase
family protein similar to SP|P32019 Type II
inositol-1,4,5-trisphosphate 5-phosphatase precursor
(EC 3.1.3.56) {Homo sapiens}; contains Pfam profile
PF03372: Endonuclease/Exonuclease/phosphatase family
Length = 334
Score = 27.9 bits (59), Expect = 5.4
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = +1
Query: 334 KLLGKKEVDISEIFKSRAPRNKSKKDYSAMLQKRNYTISADSTKTLR 474
K + ++ ++ I S PR+K K+D + L NY I S +R
Sbjct: 181 KKVDQRNTELRHIANSLLPRDKRKRDLTVWLGDLNYRIQDVSNHPVR 227
>At4g29750.1 68417.m04237 expressed protein contains Pfam domain,
PF04581: Protein of unknown function (DUF578)
Length = 776
Score = 27.5 bits (58), Expect = 7.1
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = -3
Query: 460 WSLPRWCSYVSAAWPNN 410
WS PR Y+SA W NN
Sbjct: 152 WSFPRPSGYMSAPWVNN 168
>At2g44020.1 68415.m05473 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 507
Score = 27.5 bits (58), Expect = 7.1
Identities = 10/28 (35%), Positives = 20/28 (71%)
Frame = +1
Query: 403 KKDYSAMLQKRNYTISADSTKTLRPNME 486
KK + ML+KR+Y + + +T++PN++
Sbjct: 266 KKIVARMLEKRSYIVGYNLEETVKPNVD 293
>At5g03060.1 68418.m00254 expressed protein ; expression supported
by MPSS
Length = 292
Score = 27.1 bits (57), Expect = 9.4
Identities = 13/39 (33%), Positives = 25/39 (64%), Gaps = 2/39 (5%)
Frame = +2
Query: 11 VSVKEEPPCVAQEEKVEKIKSIIRR--EFQNELEARQSE 121
+SVK + +A+EE+VEK+K + + E + +A +S+
Sbjct: 120 LSVKNQELLIAKEEEVEKLKKMENKYAELKERFDAAESQ 158
>At4g27500.1 68417.m03950 expressed protein non-consensus GA donor
splice site at exon 6
Length = 612
Score = 27.1 bits (57), Expect = 9.4
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +2
Query: 2 EVPVSVKEEPPCVAQEEKVEKIKSIIRREFQNELEARQSEVV 127
EVP + +EE QEEK +K K + +N R E +
Sbjct: 535 EVPEASEEEIEAPVQEEKPQKEKVFKEKPIRNRTRGRGPETI 576
>At3g26500.1 68416.m03305 leucine-rich repeat family protein
Length = 471
Score = 27.1 bits (57), Expect = 9.4
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +3
Query: 207 ISNSNYRMRRLKMRSLNQRPQVESRDINIATRGA 308
I +S YR+R+L+ +L+Q P +E +AT+GA
Sbjct: 362 IPDSFYRLRKLEKLNLDQNP-LEIPSQEVATQGA 394
>At3g15115.1 68416.m01912 expressed protein
Length = 339
Score = 27.1 bits (57), Expect = 9.4
Identities = 11/37 (29%), Positives = 21/37 (56%), Gaps = 3/37 (8%)
Frame = +2
Query: 29 PPCVAQEE---KVEKIKSIIRREFQNELEARQSEVVL 130
PPC+ ++E + EKI ++RR+F + ++ L
Sbjct: 85 PPCIEKKEGGGEPEKINKVMRRQFSEKTRVQERRTYL 121
>At2g25460.1 68415.m03049 expressed protein
Length = 423
Score = 27.1 bits (57), Expect = 9.4
Identities = 15/58 (25%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
Frame = +1
Query: 331 KKLLGKKEVDISEIFKSRAPRNKSKKDYSAMLQKRNYTISADSTKTLRPNM-EIKTEP 501
K L+GK +D+SE+ A + +S + ++ + +S ++T + E++TEP
Sbjct: 111 KSLIGKASLDLSEL----ASKQESTVERKLPIRSKGSVLSKEATLVVNVTFSEVRTEP 164
>At2g22360.1 68415.m02653 DNAJ heat shock family protein similar to
SP|Q9S5A3 Chaperone protein dnaJ {Listeria
monocytogenes}; contains Pfam profiles PF00226 DnaJ
domain, PF01556 DnaJ C terminal region, PF00684 DnaJ
central domain (4 repeats)
Length = 442
Score = 27.1 bits (57), Expect = 9.4
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +1
Query: 271 SKAEISTLLREGQRNIHPSVKKLLGKKE 354
+KAEI + R+ RN HP V K G +E
Sbjct: 98 TKAEIKSAYRKLARNYHPDVNKDPGAEE 125
>At2g16090.1 68415.m01845 zinc finger protein-related contains
similarity to zinc finger proteins and Pfam domain,
PF01485: IBR domain
Length = 593
Score = 27.1 bits (57), Expect = 9.4
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +2
Query: 482 WRSKLNPLQDE*TKEDPACSEAERHRADSGRGNQES 589
W S+L+ + ED +CS +R R D N ++
Sbjct: 543 WTSRLDQALESGKSEDTSCSSGKRARIDESYRNSQT 578
>At1g76600.1 68414.m08913 expressed protein
Length = 216
Score = 27.1 bits (57), Expect = 9.4
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +1
Query: 424 LQKRNYTISADSTKTLRPNMEIKTEPAAGRVDQRR 528
+ KR Y +SA L + E AAG+ ++RR
Sbjct: 91 ISKRQYRLSASDMAALAVKASVAIEKAAGKKNRRR 125
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,373,029
Number of Sequences: 28952
Number of extensions: 247908
Number of successful extensions: 752
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 736
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 752
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1180950720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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