BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0468 (762 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g23540.1 68414.m02960 protein kinase family protein contains ... 31 1.1 At5g28850.1 68418.m03549 calcium-binding EF hand family protein ... 30 1.5 At1g05410.1 68414.m00549 expressed protein 29 2.6 At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1 ... 28 7.8 >At1g23540.1 68414.m02960 protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain Length = 720 Score = 30.7 bits (66), Expect = 1.1 Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 1/61 (1%) Frame = +3 Query: 315 DTESQSNGVDNSNGTQKSQLVFHCQQAHGSPLGLISGFSNVKELYEKIAECYE-FSPEDI 491 +++ Q + + NS GT H Q+ G+P I G YE++AE + F+ ++I Sbjct: 317 NSQQQQSSMGNSYGTAGGGYPHHQMQSSGTPDSAILGSGQTHFSYEELAEITQGFARKNI 376 Query: 492 L 494 L Sbjct: 377 L 377 >At5g28850.1 68418.m03549 calcium-binding EF hand family protein contains Pfam profile: PF00036 EF hand Length = 324 Score = 30.3 bits (65), Expect = 1.5 Identities = 24/94 (25%), Positives = 48/94 (51%), Gaps = 4/94 (4%) Frame = +2 Query: 473 IFTRRYSFLYFKTHKVD----MKKLLGGQIGLEDFIFAHRKGRPKEIEIVKTEDALGLTI 640 +++R YSFL+F + + D +K+LL GLE F+ + + + + E V + I Sbjct: 5 LYSRFYSFLFFFSWQDDFKPLLKELLATHPGLE-FLQSTPEFQERYAETVTYR--IFYYI 61 Query: 641 TDNGAGYAFIKRIKEGSIVSRIPHIEVGDHIEKL 742 +G G + +K G+++ + H + + I K+ Sbjct: 62 NRSGNGRITFRELKRGNLIDAMLHADEEEDINKV 95 >At1g05410.1 68414.m00549 expressed protein Length = 471 Score = 29.5 bits (63), Expect = 2.6 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = -3 Query: 577 MRENKVF*SYLPSEKFFHIHFVSFKVQKRISS 482 +R+NKVF S L +++ +HF VQK +S Sbjct: 78 LRKNKVFRSRLSLQRYIRVHFPEADVQKFFAS 109 >At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1 subunit-related contains similarity to Swiss-Prot:P80387 5'-AMP-activated protein kinase, beta-1 subunit (AMPK beta-1 chain) (AMPKb) (40 kDa subunit) [Sus scrofa] Length = 591 Score = 27.9 bits (59), Expect = 7.8 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +2 Query: 605 IVKTEDALGLTITDNGAGYAFIKRIKEGSIVSRIPHIEVGDHIEK 739 +V E LG+ + G F+ IK+GS + I VGD ++K Sbjct: 78 MVTLEKPLGIRFALSADGKIFVHAIKKGSNAEKARIIMVGDTLKK 122 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,746,641 Number of Sequences: 28952 Number of extensions: 318205 Number of successful extensions: 762 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 747 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 762 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1702303248 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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