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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0468
         (762 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g23540.1 68414.m02960 protein kinase family protein contains ...    31   1.1  
At5g28850.1 68418.m03549 calcium-binding EF hand family protein ...    30   1.5  
At1g05410.1 68414.m00549 expressed protein                             29   2.6  
At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1 ...    28   7.8  

>At1g23540.1 68414.m02960 protein kinase family protein contains
           Pfam domain, PF00069: Protein kinase domain
          Length = 720

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 1/61 (1%)
 Frame = +3

Query: 315 DTESQSNGVDNSNGTQKSQLVFHCQQAHGSPLGLISGFSNVKELYEKIAECYE-FSPEDI 491
           +++ Q + + NS GT       H  Q+ G+P   I G       YE++AE  + F+ ++I
Sbjct: 317 NSQQQQSSMGNSYGTAGGGYPHHQMQSSGTPDSAILGSGQTHFSYEELAEITQGFARKNI 376

Query: 492 L 494
           L
Sbjct: 377 L 377


>At5g28850.1 68418.m03549 calcium-binding EF hand family protein
           contains Pfam profile: PF00036 EF hand
          Length = 324

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 24/94 (25%), Positives = 48/94 (51%), Gaps = 4/94 (4%)
 Frame = +2

Query: 473 IFTRRYSFLYFKTHKVD----MKKLLGGQIGLEDFIFAHRKGRPKEIEIVKTEDALGLTI 640
           +++R YSFL+F + + D    +K+LL    GLE F+ +  + + +  E V     +   I
Sbjct: 5   LYSRFYSFLFFFSWQDDFKPLLKELLATHPGLE-FLQSTPEFQERYAETVTYR--IFYYI 61

Query: 641 TDNGAGYAFIKRIKEGSIVSRIPHIEVGDHIEKL 742
             +G G    + +K G+++  + H +  + I K+
Sbjct: 62  NRSGNGRITFRELKRGNLIDAMLHADEEEDINKV 95


>At1g05410.1 68414.m00549 expressed protein
          Length = 471

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 13/32 (40%), Positives = 20/32 (62%)
 Frame = -3

Query: 577 MRENKVF*SYLPSEKFFHIHFVSFKVQKRISS 482
           +R+NKVF S L  +++  +HF    VQK  +S
Sbjct: 78  LRKNKVFRSRLSLQRYIRVHFPEADVQKFFAS 109


>At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1
           subunit-related contains similarity to Swiss-Prot:P80387
           5'-AMP-activated protein kinase, beta-1 subunit (AMPK
           beta-1 chain) (AMPKb) (40 kDa subunit) [Sus scrofa]
          Length = 591

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 15/45 (33%), Positives = 23/45 (51%)
 Frame = +2

Query: 605 IVKTEDALGLTITDNGAGYAFIKRIKEGSIVSRIPHIEVGDHIEK 739
           +V  E  LG+    +  G  F+  IK+GS   +   I VGD ++K
Sbjct: 78  MVTLEKPLGIRFALSADGKIFVHAIKKGSNAEKARIIMVGDTLKK 122


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,746,641
Number of Sequences: 28952
Number of extensions: 318205
Number of successful extensions: 762
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 747
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 762
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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