Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0461
         (643 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc...    87   3e-18
SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc...    82   6e-17
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    29   0.43 
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S...    28   1.00 
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce...    26   4.0  

>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score = 86.6 bits (205), Expect = 3e-18
 Identities = 41/59 (69%), Positives = 48/59 (81%)
 Frame = +1

Query: 19  APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLR 195
           AP    +KK KK+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLR
Sbjct: 8   APVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLR 66



 Score = 70.5 bits (165), Expect = 2e-13
 Identities = 29/41 (70%), Positives = 36/41 (87%)
 Frame = +1

Query: 517 RKSEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAI 639
           RKSE+EYYA+L++  VHHYSGNNI+LGTACGK +RV  LA+
Sbjct: 66  RKSELEYYAMLSRCSVHHYSGNNIDLGTACGKLFRVGVLAV 106


>SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 109

 Score = 82.2 bits (194), Expect = 6e-17
 Identities = 38/55 (69%), Positives = 45/55 (81%)
 Frame = +1

Query: 31  VAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLR 195
           V  KK KK+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLR
Sbjct: 4   VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLR 58



 Score = 70.9 bits (166), Expect = 1e-13
 Identities = 30/42 (71%), Positives = 36/42 (85%)
 Frame = +1

Query: 517 RKSEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAIT 642
           RKSE+EYYA+L+K  VHHY+G NI+LGTACGK +RV  LAIT
Sbjct: 58  RKSELEYYAMLSKANVHHYAGTNIDLGTACGKLFRVGVLAIT 99


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 29.5 bits (63), Expect = 0.43
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = +1

Query: 43  KQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVI 168
           ++KK I+     ++L   SGKYC G   T+  L++G  + ++
Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLL 318


>SPBC336.05c |||S-adenosylmethionine-
           dependentmethyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 378

 Score = 28.3 bits (60), Expect = 1.00
 Identities = 19/59 (32%), Positives = 29/59 (49%)
 Frame = -3

Query: 302 ANCFTKPQRESILLCQKTPYASL*QVLGNSTSQ*PHLSGGAFLAMITSFALPCRRVFKV 126
           ++CF K Q  SILL +K  Y  + Q+   +  Q  +L   AF   +  F   C  +F+V
Sbjct: 245 SHCFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDHL--FGRHCLLLFQV 301


>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 154

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 17/58 (29%), Positives = 32/58 (55%)
 Frame = +1

Query: 19  APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPL 192
           APK +  KK  KT++  + +  ++        G K+ +K +R+G+  LVI+A +  P+
Sbjct: 33  APKKLN-KKMMKTVKKASKQKHILR-------GVKEVVKAVRKGEKGLVILAGDISPM 82


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,683,338
Number of Sequences: 5004
Number of extensions: 53317
Number of successful extensions: 115
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)