Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0452
(609 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putati... 128 2e-30
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati... 124 3e-29
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati... 124 3e-29
At1g59650.1 68414.m06709 expressed protein ; supporting cDNA gi|... 30 1.4
At5g53030.2 68418.m06586 expressed protein 29 2.4
At5g53030.1 68418.m06587 expressed protein 29 2.4
At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical... 28 5.6
At3g52890.2 68416.m05829 protein kinase (KIPK) identical to prot... 28 5.6
At3g52890.1 68416.m05828 protein kinase (KIPK) identical to prot... 28 5.6
At3g15750.1 68416.m01995 expressed protein 28 5.6
At5g44290.1 68418.m05421 protein kinase family protein contains ... 27 9.7
At3g61570.1 68416.m06896 intracellular protein transport protein... 27 9.7
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 27 9.7
>At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putative
strong similarity to SP|Q43175 Citrate synthase,
mitochondrial precursor {Solanum tuberosum}; contains
Pfam profile PF00285: Citrate synthase
Length = 433
Score = 128 bits (310), Expect = 2e-30
Identities = 58/83 (69%), Positives = 69/83 (83%)
Frame = +3
Query: 255 MMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTG 434
M+ GGMRG+ GL+WETS+LDADEGIRFRG+SIPECQ+ LP A+ GEEPLPE L WLL+TG
Sbjct: 40 MVLGGMRGMTGLLWETSLLDADEGIRFRGMSIPECQKILPSAESGEEPLPESLLWLLLTG 99
Query: 435 DIPTEAQAKALSKEWAARAELPA 503
+PT+ QA ALS E A RA +PA
Sbjct: 100 KVPTKEQANALSTELAHRAAVPA 122
Score = 33.9 bits (74), Expect = 0.085
Identities = 14/33 (42%), Positives = 22/33 (66%)
Frame = +2
Query: 509 VTMLNNMPGKLHPMSQFSAAVTALNSESKFAKA 607
V ++ +P HPM+QF++ V AL +S+F KA
Sbjct: 120 VPAIDALPSTAHPMTQFASGVMALQVQSEFQKA 152
Score = 27.1 bits (57), Expect = 9.7
Identities = 9/25 (36%), Positives = 20/25 (80%)
Frame = +1
Query: 151 NLKSILQEKIPKEQEKIREFRKKHG 225
+LKS +QE IP++Q+++++ + + G
Sbjct: 5 DLKSQMQEIIPEQQDRLKKLKSEQG 29
>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 474
Score = 124 bits (300), Expect = 3e-29
Identities = 57/98 (58%), Positives = 73/98 (74%)
Frame = +3
Query: 255 MMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTG 434
M+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+ G EPLPEGL WLL+TG
Sbjct: 75 MVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWLLLTG 134
Query: 435 DIPTEAQAKALSKEWAARAELPAHV*QC*TICPANCIP 548
+P++ Q +ALSK+ A RA +P +V P+ P
Sbjct: 135 KVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHP 172
Score = 34.3 bits (75), Expect = 0.064
Identities = 16/61 (26%), Positives = 35/61 (57%)
Frame = +1
Query: 52 MALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGST 231
++ F SR+ Q + + ++ S+ +LKS LQE IP++Q+++++ + +HG
Sbjct: 7 VSAFTRLRSRVQGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKV 66
Query: 232 K 234
+
Sbjct: 67 Q 67
Score = 33.1 bits (72), Expect = 0.15
Identities = 13/30 (43%), Positives = 21/30 (70%)
Frame = +2
Query: 518 LNNMPGKLHPMSQFSAAVTALNSESKFAKA 607
++ +P HPM+QF++ V AL +S+F KA
Sbjct: 163 IDALPSTAHPMTQFASGVMALQVQSEFQKA 192
>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 473
Score = 124 bits (300), Expect = 3e-29
Identities = 57/98 (58%), Positives = 73/98 (74%)
Frame = +3
Query: 255 MMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTG 434
M+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+ G EPLPEGL WLL+TG
Sbjct: 74 MVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWLLLTG 133
Query: 435 DIPTEAQAKALSKEWAARAELPAHV*QC*TICPANCIP 548
+P++ Q +ALSK+ A RA +P +V P+ P
Sbjct: 134 KVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHP 171
Score = 33.1 bits (72), Expect = 0.15
Identities = 12/33 (36%), Positives = 24/33 (72%)
Frame = +1
Query: 136 SAEQTNLKSILQEKIPKEQEKIREFRKKHGSTK 234
S+ +LKS LQE IP++Q+++++ + +HG +
Sbjct: 34 SSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQ 66
Score = 33.1 bits (72), Expect = 0.15
Identities = 13/30 (43%), Positives = 21/30 (70%)
Frame = +2
Query: 518 LNNMPGKLHPMSQFSAAVTALNSESKFAKA 607
++ +P HPM+QF++ V AL +S+F KA
Sbjct: 162 IDALPSTAHPMTQFASGVMALQVQSEFQKA 191
>At1g59650.1 68414.m06709 expressed protein ; supporting cDNA
gi|6520232|dbj|AB028233.1|
Length = 492
Score = 29.9 bits (64), Expect = 1.4
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = +3
Query: 525 ICPANCIPCHSSRLPSPHSTVNLNSLKP 608
I P+NC+PC +S +PS +L+S P
Sbjct: 157 ILPSNCLPCLNSTVPSIEKRRSLSSSPP 184
>At5g53030.2 68418.m06586 expressed protein
Length = 224
Score = 29.1 bits (62), Expect = 2.4
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +3
Query: 300 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 407
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At5g53030.1 68418.m06587 expressed protein
Length = 245
Score = 29.1 bits (62), Expect = 2.4
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +3
Query: 300 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 407
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical to
EIN2 [Arabidopsis thaliana] gi|5231113|gb|AAD41076;
member of the natural resistance-associated macrophage
protein (NRAMP) metal transporter family, PMID:11500563;
metal transport capacity has not been shown,
PMID:11500563, PMID:1038174
Length = 1294
Score = 27.9 bits (59), Expect = 5.6
Identities = 20/68 (29%), Positives = 30/68 (44%), Gaps = 1/68 (1%)
Frame = -3
Query: 220 ASFGIRGSSLVPWESFPGEYFLNSSVQRLDLEVELSQSDMLSAILRVLKM*S*IEPFLKP 41
A G+ G+S+VP + YF S D++ D L AI V S + L
Sbjct: 198 ALMGLLGASIVPHNFYIHSYFAGESTSSSDVDKSSLCQDHLFAIFGVFSGLSLVNYVLMN 257
Query: 40 PSAN-YHT 20
+AN +H+
Sbjct: 258 AAANVFHS 265
>At3g52890.2 68416.m05829 protein kinase (KIPK) identical to protein
kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 934
Score = 27.9 bits (59), Expect = 5.6
Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
Frame = +3
Query: 336 RGLSIPECQQQLPKAKGGEE----PLPEGLFWLLVTGDIPTE 449
RGL + E + +L KG E P EGL W L+ IP E
Sbjct: 853 RGLLVKEPENRLGSEKGSVEIKRHPFFEGLNWALIRCAIPPE 894
>At3g52890.1 68416.m05828 protein kinase (KIPK) identical to protein
kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 934
Score = 27.9 bits (59), Expect = 5.6
Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
Frame = +3
Query: 336 RGLSIPECQQQLPKAKGGEE----PLPEGLFWLLVTGDIPTE 449
RGL + E + +L KG E P EGL W L+ IP E
Sbjct: 853 RGLLVKEPENRLGSEKGSVEIKRHPFFEGLNWALIRCAIPPE 894
>At3g15750.1 68416.m01995 expressed protein
Length = 186
Score = 27.9 bits (59), Expect = 5.6
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = +1
Query: 121 LLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGS 228
L+RG+S+ L L+EK+ +QE +F+K H S
Sbjct: 79 LVRGVSSALAFLPDELREKLIDKQETREKFQKLHSS 114
>At5g44290.1 68418.m05421 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 644
Score = 27.1 bits (57), Expect = 9.7
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = -2
Query: 521 STLSHVSR*LRPRCPFFRQRFSLRFGGYIASDEK 420
S L++V PR P +R + + GGY+ SD++
Sbjct: 533 SRLTYVKDNAAPRIPSYRANSAGQGGGYVGSDQQ 566
>At3g61570.1 68416.m06896 intracellular protein transport protein
USO1-related contains weak similarity to intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]
Length = 712
Score = 27.1 bits (57), Expect = 9.7
Identities = 10/26 (38%), Positives = 20/26 (76%)
Frame = -3
Query: 181 ESFPGEYFLNSSVQRLDLEVELSQSD 104
ESFPG+ + S+QRL+++++ +Q +
Sbjct: 336 ESFPGKEEMEQSLQRLEMDLKETQRE 361
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 27.1 bits (57), Expect = 9.7
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -3
Query: 346 DRPRKRIPSSASSTEVSQTRPLIPRMP 266
DR R+R PSS+ S S++ P++ R P
Sbjct: 604 DRRRRRSPSSSRSPSRSRSPPVLHRSP 630
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,618,513
Number of Sequences: 28952
Number of extensions: 247593
Number of successful extensions: 857
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 836
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 857
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1216725696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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