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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0452
         (609 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putati...   128   2e-30
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati...   124   3e-29
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati...   124   3e-29
At1g59650.1 68414.m06709 expressed protein ; supporting cDNA gi|...    30   1.4  
At5g53030.2 68418.m06586 expressed protein                             29   2.4  
At5g53030.1 68418.m06587 expressed protein                             29   2.4  
At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical...    28   5.6  
At3g52890.2 68416.m05829 protein kinase (KIPK) identical to prot...    28   5.6  
At3g52890.1 68416.m05828 protein kinase (KIPK) identical to prot...    28   5.6  
At3g15750.1 68416.m01995 expressed protein                             28   5.6  
At5g44290.1 68418.m05421 protein kinase family protein contains ...    27   9.7  
At3g61570.1 68416.m06896 intracellular protein transport protein...    27   9.7  
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p...    27   9.7  

>At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putative
           strong similarity to SP|Q43175 Citrate synthase,
           mitochondrial precursor {Solanum tuberosum}; contains
           Pfam profile PF00285: Citrate synthase
          Length = 433

 Score =  128 bits (310), Expect = 2e-30
 Identities = 58/83 (69%), Positives = 69/83 (83%)
 Frame = +3

Query: 255 MMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTG 434
           M+ GGMRG+ GL+WETS+LDADEGIRFRG+SIPECQ+ LP A+ GEEPLPE L WLL+TG
Sbjct: 40  MVLGGMRGMTGLLWETSLLDADEGIRFRGMSIPECQKILPSAESGEEPLPESLLWLLLTG 99

Query: 435 DIPTEAQAKALSKEWAARAELPA 503
            +PT+ QA ALS E A RA +PA
Sbjct: 100 KVPTKEQANALSTELAHRAAVPA 122



 Score = 33.9 bits (74), Expect = 0.085
 Identities = 14/33 (42%), Positives = 22/33 (66%)
 Frame = +2

Query: 509 VTMLNNMPGKLHPMSQFSAAVTALNSESKFAKA 607
           V  ++ +P   HPM+QF++ V AL  +S+F KA
Sbjct: 120 VPAIDALPSTAHPMTQFASGVMALQVQSEFQKA 152



 Score = 27.1 bits (57), Expect = 9.7
 Identities = 9/25 (36%), Positives = 20/25 (80%)
 Frame = +1

Query: 151 NLKSILQEKIPKEQEKIREFRKKHG 225
           +LKS +QE IP++Q+++++ + + G
Sbjct: 5   DLKSQMQEIIPEQQDRLKKLKSEQG 29


>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
           strong similarity to SP|P20115 Citrate synthase,
           mitochondrial precursor  {Arabidopsis thaliana};
           contains Pfam profile PF00285: Citrate synthase
          Length = 474

 Score =  124 bits (300), Expect = 3e-29
 Identities = 57/98 (58%), Positives = 73/98 (74%)
 Frame = +3

Query: 255 MMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTG 434
           M+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+ G EPLPEGL WLL+TG
Sbjct: 75  MVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWLLLTG 134

Query: 435 DIPTEAQAKALSKEWAARAELPAHV*QC*TICPANCIP 548
            +P++ Q +ALSK+ A RA +P +V       P+   P
Sbjct: 135 KVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHP 172



 Score = 34.3 bits (75), Expect = 0.064
 Identities = 16/61 (26%), Positives = 35/61 (57%)
 Frame = +1

Query: 52  MALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGST 231
           ++ F    SR+   Q +   +   ++  S+   +LKS LQE IP++Q+++++ + +HG  
Sbjct: 7   VSAFTRLRSRVQGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKV 66

Query: 232 K 234
           +
Sbjct: 67  Q 67



 Score = 33.1 bits (72), Expect = 0.15
 Identities = 13/30 (43%), Positives = 21/30 (70%)
 Frame = +2

Query: 518 LNNMPGKLHPMSQFSAAVTALNSESKFAKA 607
           ++ +P   HPM+QF++ V AL  +S+F KA
Sbjct: 163 IDALPSTAHPMTQFASGVMALQVQSEFQKA 192


>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
           strong similarity to SP|P20115 Citrate synthase,
           mitochondrial precursor  {Arabidopsis thaliana};
           contains Pfam profile PF00285: Citrate synthase
          Length = 473

 Score =  124 bits (300), Expect = 3e-29
 Identities = 57/98 (58%), Positives = 73/98 (74%)
 Frame = +3

Query: 255 MMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTG 434
           M+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+ G EPLPEGL WLL+TG
Sbjct: 74  MVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWLLLTG 133

Query: 435 DIPTEAQAKALSKEWAARAELPAHV*QC*TICPANCIP 548
            +P++ Q +ALSK+ A RA +P +V       P+   P
Sbjct: 134 KVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHP 171



 Score = 33.1 bits (72), Expect = 0.15
 Identities = 12/33 (36%), Positives = 24/33 (72%)
 Frame = +1

Query: 136 SAEQTNLKSILQEKIPKEQEKIREFRKKHGSTK 234
           S+   +LKS LQE IP++Q+++++ + +HG  +
Sbjct: 34  SSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQ 66



 Score = 33.1 bits (72), Expect = 0.15
 Identities = 13/30 (43%), Positives = 21/30 (70%)
 Frame = +2

Query: 518 LNNMPGKLHPMSQFSAAVTALNSESKFAKA 607
           ++ +P   HPM+QF++ V AL  +S+F KA
Sbjct: 162 IDALPSTAHPMTQFASGVMALQVQSEFQKA 191


>At1g59650.1 68414.m06709 expressed protein ; supporting cDNA
           gi|6520232|dbj|AB028233.1|
          Length = 492

 Score = 29.9 bits (64), Expect = 1.4
 Identities = 12/28 (42%), Positives = 18/28 (64%)
 Frame = +3

Query: 525 ICPANCIPCHSSRLPSPHSTVNLNSLKP 608
           I P+NC+PC +S +PS     +L+S  P
Sbjct: 157 ILPSNCLPCLNSTVPSIEKRRSLSSSPP 184


>At5g53030.2 68418.m06586 expressed protein
          Length = 224

 Score = 29.1 bits (62), Expect = 2.4
 Identities = 14/36 (38%), Positives = 19/36 (52%)
 Frame = +3

Query: 300 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 407
           T+VLD    +R R LS+P     + K +G   P PE
Sbjct: 98  TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133


>At5g53030.1 68418.m06587 expressed protein
          Length = 245

 Score = 29.1 bits (62), Expect = 2.4
 Identities = 14/36 (38%), Positives = 19/36 (52%)
 Frame = +3

Query: 300 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 407
           T+VLD    +R R LS+P     + K +G   P PE
Sbjct: 98  TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133


>At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical to
           EIN2 [Arabidopsis thaliana] gi|5231113|gb|AAD41076;
           member of the natural resistance-associated macrophage
           protein (NRAMP) metal transporter family, PMID:11500563;
           metal transport capacity has not been shown,
           PMID:11500563, PMID:1038174
          Length = 1294

 Score = 27.9 bits (59), Expect = 5.6
 Identities = 20/68 (29%), Positives = 30/68 (44%), Gaps = 1/68 (1%)
 Frame = -3

Query: 220 ASFGIRGSSLVPWESFPGEYFLNSSVQRLDLEVELSQSDMLSAILRVLKM*S*IEPFLKP 41
           A  G+ G+S+VP   +   YF   S    D++      D L AI  V    S +   L  
Sbjct: 198 ALMGLLGASIVPHNFYIHSYFAGESTSSSDVDKSSLCQDHLFAIFGVFSGLSLVNYVLMN 257

Query: 40  PSAN-YHT 20
            +AN +H+
Sbjct: 258 AAANVFHS 265


>At3g52890.2 68416.m05829 protein kinase (KIPK) identical to protein
           kinase KIPK (KCBP-interacting protein kinase)
           [Arabidopsis thaliana] gi|7716430|gb|AAF68383
          Length = 934

 Score = 27.9 bits (59), Expect = 5.6
 Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
 Frame = +3

Query: 336 RGLSIPECQQQLPKAKGGEE----PLPEGLFWLLVTGDIPTE 449
           RGL + E + +L   KG  E    P  EGL W L+   IP E
Sbjct: 853 RGLLVKEPENRLGSEKGSVEIKRHPFFEGLNWALIRCAIPPE 894


>At3g52890.1 68416.m05828 protein kinase (KIPK) identical to protein
           kinase KIPK (KCBP-interacting protein kinase)
           [Arabidopsis thaliana] gi|7716430|gb|AAF68383
          Length = 934

 Score = 27.9 bits (59), Expect = 5.6
 Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
 Frame = +3

Query: 336 RGLSIPECQQQLPKAKGGEE----PLPEGLFWLLVTGDIPTE 449
           RGL + E + +L   KG  E    P  EGL W L+   IP E
Sbjct: 853 RGLLVKEPENRLGSEKGSVEIKRHPFFEGLNWALIRCAIPPE 894


>At3g15750.1 68416.m01995 expressed protein 
          Length = 186

 Score = 27.9 bits (59), Expect = 5.6
 Identities = 14/36 (38%), Positives = 22/36 (61%)
 Frame = +1

Query: 121 LLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGS 228
           L+RG+S+    L   L+EK+  +QE   +F+K H S
Sbjct: 79  LVRGVSSALAFLPDELREKLIDKQETREKFQKLHSS 114


>At5g44290.1 68418.m05421 protein kinase family protein contains
           Pfam domain, PF00069: Protein kinase domain
          Length = 644

 Score = 27.1 bits (57), Expect = 9.7
 Identities = 12/34 (35%), Positives = 20/34 (58%)
 Frame = -2

Query: 521 STLSHVSR*LRPRCPFFRQRFSLRFGGYIASDEK 420
           S L++V     PR P +R   + + GGY+ SD++
Sbjct: 533 SRLTYVKDNAAPRIPSYRANSAGQGGGYVGSDQQ 566


>At3g61570.1 68416.m06896 intracellular protein transport protein
           USO1-related contains weak similarity to intracellular
           protein transport protein USO1 (Swiss-Prot:P25386)
           [Saccharomyces cerevisiae]
          Length = 712

 Score = 27.1 bits (57), Expect = 9.7
 Identities = 10/26 (38%), Positives = 20/26 (76%)
 Frame = -3

Query: 181 ESFPGEYFLNSSVQRLDLEVELSQSD 104
           ESFPG+  +  S+QRL+++++ +Q +
Sbjct: 336 ESFPGKEEMEQSLQRLEMDLKETQRE 361


>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
           protein contains Pfam profile PF01480: PWI domain
          Length = 878

 Score = 27.1 bits (57), Expect = 9.7
 Identities = 12/27 (44%), Positives = 18/27 (66%)
 Frame = -3

Query: 346 DRPRKRIPSSASSTEVSQTRPLIPRMP 266
           DR R+R PSS+ S   S++ P++ R P
Sbjct: 604 DRRRRRSPSSSRSPSRSRSPPVLHRSP 630


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,618,513
Number of Sequences: 28952
Number of extensions: 247593
Number of successful extensions: 857
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 836
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 857
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1216725696
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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