Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0445
(644 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19900.1 68415.m02326 malate oxidoreductase, putative similar... 143 8e-35
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar... 138 2e-33
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar... 136 9e-33
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar... 136 9e-33
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 104 4e-23
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar... 100 7e-22
At1g19485.1 68414.m02427 AT hook motif-containing protein contai... 29 2.0
At2g40570.1 68415.m05005 initiator tRNA phosphoribosyl transfera... 28 4.6
At5g67440.1 68418.m08503 phototropic-responsive NPH3 family prot... 28 6.1
At4g27300.1 68417.m03917 S-locus protein kinase, putative simila... 28 6.1
>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P51615) {Vitis vinifera}
Length = 581
Score = 143 bits (347), Expect = 8e-35
Identities = 62/85 (72%), Positives = 75/85 (88%)
Frame = +1
Query: 1 QKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIP 180
QKFG ++RRP+GLFI++ DKG + DVLKNWPE +++ IVVTDGERILGLGDLG GMGIP
Sbjct: 138 QKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 197
Query: 181 VGKLALYTALGGIKPHQCLPITIDV 255
VGKLALY+ALGG++P CLP+TIDV
Sbjct: 198 VGKLALYSALGGVRPSACLPVTIDV 222
Score = 112 bits (270), Expect = 2e-25
Identities = 50/85 (58%), Positives = 63/85 (74%)
Frame = +3
Query: 255 GTNTQSMLDDPLYIGLRQRRVRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRL 434
GTN + +L+D YIGLRQ+R G +Y ELL+EFM AV + YG+ L+QFEDF N NAF L
Sbjct: 223 GTNNEKLLNDEFYIGLRQKRATGQEYSELLNEFMSAVKQNYGEKVLIQFEDFANHNAFEL 282
Query: 435 LEKYRNKYCTFNDDIQGTAAVAVAG 509
L KY + + FNDDIQGTA+V +AG
Sbjct: 283 LAKYSDTHLVFNDDIQGTASVVLAG 307
>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P12628) {Phaseolus vulgaris}
Length = 588
Score = 138 bits (335), Expect = 2e-33
Identities = 60/85 (70%), Positives = 74/85 (87%)
Frame = +1
Query: 1 QKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIP 180
QK+G +YRRP+GL+I++ +KG + +VLKNWP+ ++ IVVTDGERILGLGDLG GMGIP
Sbjct: 145 QKYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIP 204
Query: 181 VGKLALYTALGGIKPHQCLPITIDV 255
VGKL+LYTALGGI+P CLPITIDV
Sbjct: 205 VGKLSLYTALGGIRPSACLPITIDV 229
Score = 104 bits (250), Expect = 4e-23
Identities = 47/85 (55%), Positives = 61/85 (71%)
Frame = +3
Query: 255 GTNTQSMLDDPLYIGLRQRRVRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRL 434
GTN + +L++ YIGL+Q+R G +Y E L EFM AV + YG+ LVQFEDF N +AF L
Sbjct: 230 GTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCAVKQNYGEKVLVQFEDFANHHAFEL 289
Query: 435 LEKYRNKYCTFNDDIQGTAAVAVAG 509
L KY + + FNDDIQGTA+V +AG
Sbjct: 290 LSKYCSSHLVFNDDIQGTASVVLAG 314
>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP|P12628) {Phaseolus vulgaris}
Length = 588
Score = 136 bits (330), Expect = 9e-33
Identities = 58/85 (68%), Positives = 75/85 (88%)
Frame = +1
Query: 1 QKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIP 180
QK+G ++R+P+GL+I++++KG + +VLKNWP+ ++ IVVTDGERILGLGDLG GMGIP
Sbjct: 145 QKYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIP 204
Query: 181 VGKLALYTALGGIKPHQCLPITIDV 255
VGKL+LYTALGGI+P CLPITIDV
Sbjct: 205 VGKLSLYTALGGIRPSACLPITIDV 229
Score = 109 bits (261), Expect = 2e-24
Identities = 50/85 (58%), Positives = 61/85 (71%)
Frame = +3
Query: 255 GTNTQSMLDDPLYIGLRQRRVRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRL 434
GTN + +L+D YIGL+QRR G +Y E L EFM AV + YG+ LVQFEDF N NAF L
Sbjct: 230 GTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCAVKQNYGEKVLVQFEDFANHNAFDL 289
Query: 435 LEKYRNKYCTFNDDIQGTAAVAVAG 509
L KY + + FNDDIQGTA+V +AG
Sbjct: 290 LSKYSDSHLVFNDDIQGTASVVLAG 314
>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
malate oxidoreductase (NADP-dependent malic enzyme)
GB:P34105 (Populus balsamifera subsp. trichocarpa)
Length = 646
Score = 136 bits (330), Expect = 9e-33
Identities = 57/85 (67%), Positives = 75/85 (88%)
Frame = +1
Query: 1 QKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIP 180
QK+G ++ RP+GLFI++ +KG +++VL+NWPE +++ IVVTDGERILGLGDLG GMGIP
Sbjct: 203 QKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIP 262
Query: 181 VGKLALYTALGGIKPHQCLPITIDV 255
VGKL+LYTALGG++P CLP+TIDV
Sbjct: 263 VGKLSLYTALGGVRPSACLPVTIDV 287
Score = 109 bits (262), Expect = 2e-24
Identities = 49/85 (57%), Positives = 61/85 (71%)
Frame = +3
Query: 255 GTNTQSMLDDPLYIGLRQRRVRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRL 434
GTN + +L+D YIGLRQRR G +Y EL+ EFM AV + YG+ ++QFEDF N NAF L
Sbjct: 288 GTNNEKLLNDEFYIGLRQRRATGEEYSELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDL 347
Query: 435 LEKYRNKYCTFNDDIQGTAAVAVAG 509
L KY + FNDDIQGTA+V +AG
Sbjct: 348 LAKYGTTHLVFNDDIQGTASVVLAG 372
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 104 bits (250), Expect = 4e-23
Identities = 45/85 (52%), Positives = 59/85 (69%)
Frame = +1
Query: 1 QKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIP 180
Q + +YRRPRG++ + DKG + ++ NWP V IV+TDG RILGLGDLG G+GIP
Sbjct: 145 QNYSGLYRRPRGMYFSAKDKGEMMSMIYNWPAPQVDMIVITDGSRILGLGDLGVQGIGIP 204
Query: 181 VGKLALYTALGGIKPHQCLPITIDV 255
+GKL +Y A GI P + LPI +DV
Sbjct: 205 IGKLDMYVAAAGINPQRVLPIMLDV 229
Score = 98.7 bits (235), Expect = 3e-21
Identities = 53/123 (43%), Positives = 70/123 (56%)
Frame = +3
Query: 141 GSGRLGRMRDGHPCGQTXXXXXXXXXXXXSVSAHHYRRGTNTQSMLDDPLYIGLRQRRVR 320
G G LG G P G+ V GTN + +L + LY+G+RQ R+
Sbjct: 192 GLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTNNEKLLQNDLYLGVRQPRLE 251
Query: 321 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQGTAAVA 500
G +Y E++DEFM A R+ + +VQFEDF AF LE+YR K+C FNDD+QGTA VA
Sbjct: 252 GEEYLEIIDEFMEAAFTRWPK-AVVQFEDFQAKWAFGTLERYRKKFCMFNDDVQGTAGVA 310
Query: 501 VAG 509
+AG
Sbjct: 311 LAG 313
>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
tuberosum}
Length = 623
Score = 100 bits (240), Expect = 7e-22
Identities = 45/85 (52%), Positives = 58/85 (68%)
Frame = +1
Query: 1 QKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIP 180
Q + ++RRPRG++ + D+G + ++ NWP V IVVTDG RILGLGDLG G+GI
Sbjct: 152 QNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIA 211
Query: 181 VGKLALYTALGGIKPHQCLPITIDV 255
VGKL LY A GI P + LP+ IDV
Sbjct: 212 VGKLDLYVAAAGINPQRVLPVMIDV 236
Score = 99 bits (238), Expect = 1e-21
Identities = 45/85 (52%), Positives = 64/85 (75%)
Frame = +3
Query: 255 GTNTQSMLDDPLYIGLRQRRVRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRL 434
GTN + + +DP+Y+GL+QRR+ DY +++DEFM AV R+ + +VQFEDF + AF+L
Sbjct: 237 GTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRW-PHVIVQFEDFQSKWAFKL 295
Query: 435 LEKYRNKYCTFNDDIQGTAAVAVAG 509
L++YR Y FNDD+QGTA VA+AG
Sbjct: 296 LQRYRCTYRMFNDDVQGTAGVAIAG 320
>At1g19485.1 68414.m02427 AT hook motif-containing protein contains
Pfam profile: PF00730 HhH-GPD superfamily base excision
DNA repair protein; contains Pfam PF02178: AT hook
motif; contains Pfam PF00400: WD domain, G-beta repeat
(5 copies); contains Prosite PS00354: HMG-I and HMG-Y
DNA-binding domain (A+T-hook)
Length = 815
Score = 29.5 bits (63), Expect = 2.0
Identities = 13/49 (26%), Positives = 26/49 (53%)
Frame = -3
Query: 270 TGCSYHVDSDGQTLMRLDASERGVEREFAHRDAHPACAQVAQTQNTFSV 124
TG + + +DG ++ + + + VE++ +R H C Q+ +TF V
Sbjct: 603 TGIAAYCTADG-SIFHFELTTKAVEKDTRNRTPHYLCGQLTMKDSTFIV 650
>At2g40570.1 68415.m05005 initiator tRNA phosphoribosyl transferase
family protein contains Pfam profile: PF04179 initiator
tRNA phosphoribosyl transferase
Length = 521
Score = 28.3 bits (60), Expect = 4.6
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +3
Query: 555 SSSSREPERPLWVSRNCALW 614
+S R+P RPLWVS+ +W
Sbjct: 208 ASCLRKPLRPLWVSQKTVIW 227
>At5g67440.1 68418.m08503 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 579
Score = 27.9 bits (59), Expect = 6.1
Identities = 13/58 (22%), Positives = 26/58 (44%)
Frame = +3
Query: 333 DELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQGTAAVAVA 506
+ L+DEF+ +R +C D AN +L++ Y + ++ T + +A
Sbjct: 345 ETLIDEFVTQTEKRDELDCSDDINDSSKANVAKLIDGYLAEISRIETNLSTTKFITIA 402
>At4g27300.1 68417.m03917 S-locus protein kinase, putative similar
to receptor protein kinase [Ipomoea trifida]
gi|836954|gb|AAC23542; contains S-locus glycoprotein
family domain, Pfam:PF00954
Length = 815
Score = 27.9 bits (59), Expect = 6.1
Identities = 21/80 (26%), Positives = 30/80 (37%)
Frame = +3
Query: 279 DDPLYIGLRQRRVRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKY 458
DD Y+ R GP Y L++ V+R N E+F N ++RN
Sbjct: 498 DDFSYVNFLGRGGFGPVYKGKLEDGQEIAVKRLSANSGQGVEEFKNEVKLIAKLQHRNLV 557
Query: 459 CTFNDDIQGTAAVAVAGYSP 518
IQG + + Y P
Sbjct: 558 RLLGCCIQGEECMLIYEYMP 577
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,624,727
Number of Sequences: 28952
Number of extensions: 255421
Number of successful extensions: 858
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 833
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 855
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1334473344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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