Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0441
(751 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 52 4e-07
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 50 1e-06
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 48 9e-06
At1g72970.1 68414.m08439 glucose-methanol-choline (GMC) oxidored... 47 1e-05
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored... 45 6e-05
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 44 8e-05
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 44 1e-04
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 42 4e-04
At3g30841.1 68416.m03967 2,3-biphosphoglycerate-independent phos... 33 0.27
At4g09520.1 68417.m01565 2,3-biphosphoglycerate-independent phos... 32 0.35
At1g27510.1 68414.m03353 expressed protein 28 7.6
At1g04730.1 68414.m00469 AAA-type ATPase family protein contains... 28 7.6
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 52.0 bits (119), Expect = 4e-07
Identities = 29/68 (42%), Positives = 40/68 (58%), Gaps = 7/68 (10%)
Frame = +2
Query: 80 IALFSHVTKVLIDKDTKRAYGVEFLR-DGTQQVVYA------KREVILAAGAIASPQLLM 238
+ L + V ++ D + RA GV F+ DG Y + EVILAAGA+ SPQ+L+
Sbjct: 219 VLLNATVKSIIFDANKTRAVGVRFMESDGNSSKSYKVHVEQHRGEVILAAGALGSPQILL 278
Query: 239 LSGVGPSN 262
LSG+GP N
Sbjct: 279 LSGIGPEN 286
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 50.4 bits (115), Expect = 1e-06
Identities = 30/69 (43%), Positives = 40/69 (57%), Gaps = 8/69 (11%)
Frame = +2
Query: 80 IALFSHVTKVLIDKDTK-RAYGVEFLR-DGTQQVVYA------KREVILAAGAIASPQLL 235
+ L + V ++ D K RA GV F+ DG Y + EVILAAGA+ SPQ+L
Sbjct: 198 VLLNTTVKSIIFDSSNKTRAVGVRFMESDGNSSKSYKVHVEQHRGEVILAAGALGSPQIL 257
Query: 236 MLSGVGPSN 262
+LSG+GP N
Sbjct: 258 LLSGIGPEN 266
Score = 27.9 bits (59), Expect = 7.6
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +3
Query: 669 PKSRGFIKLRSTNPLDYP 722
PKS+G +KL STNP + P
Sbjct: 344 PKSKGRLKLNSTNPRENP 361
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 47.6 bits (108), Expect = 9e-06
Identities = 38/100 (38%), Positives = 54/100 (54%), Gaps = 10/100 (10%)
Frame = +2
Query: 11 RRGTRCSTAKAFLRPVRLRQNLHIALFSHVTKVLIDKDTK------RAYGVEFLRDGTQQ 172
R G R S+A LR R N+ +A+++ V +VL+ A GV + RD +
Sbjct: 217 RTGRRHSSAD-LLRYAR-SSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVY-RDQLGR 273
Query: 173 VVYA----KREVILAAGAIASPQLLMLSGVGPSNT*KRWG 280
+A + EVIL+AGA+ SPQLL LSG+GP + WG
Sbjct: 274 FHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWG 313
>At1g72970.1 68414.m08439 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 594
Score = 47.2 bits (107), Expect = 1e-05
Identities = 31/76 (40%), Positives = 45/76 (59%), Gaps = 7/76 (9%)
Frame = +2
Query: 68 QNLHIALFSHVTKVLIDKD--TKRAYGVEFLRD-GTQ-QVVYAKR---EVILAAGAIASP 226
Q L + +++ V K++ D R GV F + G Q Q + + R EVIL++GAI SP
Sbjct: 240 QKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSP 299
Query: 227 QLLMLSGVGPSNT*KR 274
Q+LMLSG+GP +R
Sbjct: 300 QMLMLSGIGPKKELQR 315
>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 577
Score = 44.8 bits (101), Expect = 6e-05
Identities = 21/23 (91%), Positives = 23/23 (100%)
Frame = +2
Query: 191 EVILAAGAIASPQLLMLSGVGPS 259
EVIL+AGAIASPQLLMLSGVGP+
Sbjct: 274 EVILSAGAIASPQLLMLSGVGPA 296
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 44.4 bits (100), Expect = 8e-05
Identities = 30/71 (42%), Positives = 42/71 (59%), Gaps = 8/71 (11%)
Frame = +2
Query: 71 NLHIALFSHVTKVLID---KDTKRAYGVEFL-RDGTQQVVYAKR----EVILAAGAIASP 226
N+ + L + V K+L + +AYGV F +G ++ EVIL+AGAI SP
Sbjct: 224 NIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSP 283
Query: 227 QLLMLSGVGPS 259
QLLMLSG+GP+
Sbjct: 284 QLLMLSGIGPA 294
Score = 30.3 bits (65), Expect = 1.4
Identities = 23/66 (34%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = +3
Query: 528 MMTSCSTPSDGGTQVKKAHGLTDEFYNEVFQEVNNKDVFG-IFPMMLRPKSRGFIKLRST 704
M TS +T + T +TD F+N + +N G I + P SRG ++LR+T
Sbjct: 376 MQTSRTTSTTSPTL--STQSITD-FFNPINPLLNATTRAGLILQKIAGPISRGHLELRNT 432
Query: 705 NPLDYP 722
NP D P
Sbjct: 433 NPDDNP 438
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 43.6 bits (98), Expect = 1e-04
Identities = 20/26 (76%), Positives = 23/26 (88%)
Frame = +2
Query: 182 AKREVILAAGAIASPQLLMLSGVGPS 259
A EVIL+AGA+ SPQLLMLSGVGP+
Sbjct: 290 ATNEVILSAGALGSPQLLMLSGVGPA 315
Score = 29.5 bits (63), Expect = 2.5
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = +3
Query: 648 IFPMMLRPKSRGFIKLRSTNPLDYP 722
IF + P S+G +KLR+TNP D P
Sbjct: 430 IFQKVDGPASKGHMKLRNTNPRDNP 454
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 41.9 bits (94), Expect = 4e-04
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = +2
Query: 182 AKREVILAAGAIASPQLLMLSGVGPS 259
A E+IL+AG + SPQLLMLSGVGPS
Sbjct: 269 ALSEIILSAGTLGSPQLLMLSGVGPS 294
>At3g30841.1 68416.m03967 2,3-biphosphoglycerate-independent
phosphoglycerate mutase-related /
phosphoglyceromutase-related contains weak similarity to
2,3-bisphosphoglycerate-independent phosphoglycerate
mutase 1 (EC 5.4.2.1) (Phosphoglyceromutase 1)
(BPG-independent PGAM 1) (aPGAM 1) (aPGAM-Mj1).
(Swiss-Prot:Q59007) [Methanococcus jannaschii]
Length = 495
Score = 32.7 bits (71), Expect = 0.27
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = +3
Query: 162 ALSKSFMQNEKLYWRLEQ*LRLNYLCCLVSDRATPKRGGD 281
A+ K+ Q KL W+ E Y C+ D +TP GD
Sbjct: 356 AVDKAIRQLAKLLWQAESSTHYQYFLCVTGDHSTPVEYGD 395
>At4g09520.1 68417.m01565 2,3-biphosphoglycerate-independent
phosphoglycerate mutase family protein /
phosphoglyceromutase family protein contains similarity
to 2,3-bisphosphoglycerate-independent phosphoglycerate
mutase 1 (EC 5.4.2.1) (Phosphoglyceromutase 1)
(BPG-independent PGAM 1) (aPGAM 1) (aPGAM-Mj1).
(Swiss-Prot:Q59007) [Methanococcus jannaschii]; contains
weak hit to Pfam profile PF01676: Metalloenzyme
superfamily
Length = 492
Score = 32.3 bits (70), Expect = 0.35
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = +3
Query: 162 ALSKSFMQNEKLYWRLEQ*LRLNYLCCLVSDRATPKRGGD 281
A+ K+ Q KL W+ E Y C+ D +TP GD
Sbjct: 352 AVDKAIRQLAKLLWQAESSSNYQYFLCVTGDHSTPVEYGD 391
>At1g27510.1 68414.m03353 expressed protein
Length = 651
Score = 27.9 bits (59), Expect = 7.6
Identities = 19/54 (35%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +1
Query: 157 RWHSASRLC-KTRSYIGGWSNSFASITYVVWCRTEQHLKEVGIDVIHDSPGVGR 315
R+H ASRLC +T S + GW W + +E ++H +PGVGR
Sbjct: 156 RYHDASRLCNETGSGLVGW-----------WVGLPRDSEEPFGRIVHITPGVGR 198
>At1g04730.1 68414.m00469 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family ('A'TPases
'A'ssociated with diverse cellular 'A'ctivities)
Length = 954
Score = 27.9 bits (59), Expect = 7.6
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +1
Query: 370 VSLVMNRLVNINSALRYAITEDGPLTSSIGLEVVA 474
VS V+NRL+ +N L+Y +G S L +A
Sbjct: 494 VSRVVNRLICVNDRLKYICNMEGMKARSFALSALA 528
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,658,236
Number of Sequences: 28952
Number of extensions: 315659
Number of successful extensions: 758
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 737
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 758
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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