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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0434
         (560 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_30236| Best HMM Match : No HMM Matches (HMM E-Value=.)             102   2e-22
SB_8302| Best HMM Match : G-alpha (HMM E-Value=0)                      75   5e-14
SB_977| Best HMM Match : No HMM Matches (HMM E-Value=.)                74   7e-14
SB_19713| Best HMM Match : No HMM Matches (HMM E-Value=.)              60   2e-09
SB_14105| Best HMM Match : G-alpha (HMM E-Value=0)                     47   9e-06
SB_4844| Best HMM Match : G-alpha (HMM E-Value=3.6e-10)                46   2e-05
SB_58122| Best HMM Match : No HMM Matches (HMM E-Value=.)              34   0.069
SB_11257| Best HMM Match : GCC2_GCC3 (HMM E-Value=2.7e-11)             31   0.85 
SB_23785| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.1  
SB_6662| Best HMM Match : DUF755 (HMM E-Value=3.4)                     30   1.1  
SB_58528| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.0  
SB_5939| Best HMM Match : BAH (HMM E-Value=7.9e-18)                    29   2.0  
SB_5496| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   2.0  
SB_42044| Best HMM Match : ubiquitin (HMM E-Value=1.2e-06)             29   3.4  
SB_59642| Best HMM Match : DUF822 (HMM E-Value=5)                      28   4.5  
SB_39571| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   4.5  
SB_39561| Best HMM Match : ELMO_CED12 (HMM E-Value=2.52234e-44)        28   4.5  
SB_30234| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   4.5  
SB_19357| Best HMM Match : Metallothio_5 (HMM E-Value=3.4)             28   4.5  
SB_37508| Best HMM Match : S-antigen (HMM E-Value=4.3)                 28   4.5  
SB_32678| Best HMM Match : HEAT (HMM E-Value=9.6e-18)                  28   4.5  
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)                        28   4.5  
SB_3749| Best HMM Match : No HMM Matches (HMM E-Value=.)               28   6.0  
SB_15458| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.0  
SB_50768| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   7.9  
SB_27074| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   7.9  
SB_540| Best HMM Match : No HMM Matches (HMM E-Value=.)                27   7.9  
SB_24713| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   7.9  
SB_21990| Best HMM Match : DUF828 (HMM E-Value=1.4)                    27   7.9  
SB_3271| Best HMM Match : PTPS (HMM E-Value=6.6)                       27   7.9  

>SB_30236| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 353

 Score =  102 bits (244), Expect = 2e-22
 Identities = 50/87 (57%), Positives = 63/87 (72%)
 Frame = +3

Query: 180 SKGTKKDQSRNREATQKGQTRCQKSLKLLLLGTGESGKSTFIRQMRIIHGSGYSDEDKRG 359
           +K  K+  +   +  ++ +   ++ LKLLLLGTGESGKST I+QMRIIHG GYSDEDKRG
Sbjct: 9   AKERKRINAEIEKQIRQDKRNQRRELKLLLLGTGESGKSTSIKQMRIIHGQGYSDEDKRG 68

Query: 360 FIKLVYQNIFMAMQSMIRAMDLLTIQY 440
           +I LVYQNI  AM S+ RAM+ L I Y
Sbjct: 69  YIPLVYQNIITAMHSLTRAMETLKIPY 95



 Score = 56.4 bits (130), Expect = 1e-08
 Identities = 23/32 (71%), Positives = 30/32 (93%)
 Frame = +1

Query: 157 MECCMSEEAKEQKRINQEIERQLRKDKRDARR 252
           M+CC+SEEAKE+KRIN EIE+Q+R+DKR+ RR
Sbjct: 1   MDCCLSEEAKERKRINAEIEKQIRQDKRNQRR 32


>SB_8302| Best HMM Match : G-alpha (HMM E-Value=0)
          Length = 315

 Score = 74.5 bits (175), Expect = 5e-14
 Identities = 35/69 (50%), Positives = 48/69 (69%)
 Frame = +3

Query: 240 RCQKSLKLLLLGTGESGKSTFIRQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAM 419
           R ++ +KLLLLG GESGKST ++QMRIIH  G+S ED   +  LV+ N   ++ ++IRAM
Sbjct: 29  RAERDVKLLLLGAGESGKSTIVKQMRIIHDHGFSPEDYIQYRPLVFSNTIYSLLTIIRAM 88

Query: 420 DLLTIQYGN 446
           D L I + N
Sbjct: 89  DKLRIDFEN 97


>SB_977| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 765

 Score = 74.1 bits (174), Expect = 7e-14
 Identities = 34/69 (49%), Positives = 49/69 (71%)
 Frame = +3

Query: 240 RCQKSLKLLLLGTGESGKSTFIRQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAM 419
           R  + +KLLLLG GESGKST ++QM+IIH  GYS E+   + ++VY N   +M ++IRAM
Sbjct: 29  RAAREVKLLLLGAGESGKSTIVKQMKIIHEHGYSTEECTQYKQVVYSNTIQSMIAIIRAM 88

Query: 420 DLLTIQYGN 446
             L I++G+
Sbjct: 89  GTLKIEFGH 97


>SB_19713| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 507

 Score = 59.7 bits (138), Expect = 2e-09
 Identities = 32/74 (43%), Positives = 46/74 (62%)
 Frame = +3

Query: 219 ATQKGQTRCQKSLKLLLLGTGESGKSTFIRQMRIIHGSGYSDEDKRGFIKLVYQNIFMAM 398
           A +K Q R Q  +K+LLLG GESGKSTF++QMRII+G  +  +  + +  +VY NI   M
Sbjct: 235 AQEKAQLRRQ--VKILLLGAGESGKSTFLKQMRIIYGKDFDADALQEYKPVVYSNILKGM 292

Query: 399 QSMIRAMDLLTIQY 440
           + +  A   L I +
Sbjct: 293 KVLADARRKLRIDW 306



 Score = 32.7 bits (71), Expect = 0.21
 Identities = 11/30 (36%), Positives = 22/30 (73%)
 Frame = +1

Query: 163 CCMSEEAKEQKRINQEIERQLRKDKRDARR 252
           CC+++E  EQ+  +Q+I++Q+ ++K   RR
Sbjct: 214 CCLTQEEAEQRAQSQKIDKQIAQEKAQLRR 243


>SB_14105| Best HMM Match : G-alpha (HMM E-Value=0)
          Length = 320

 Score = 47.2 bits (107), Expect = 9e-06
 Identities = 23/85 (27%), Positives = 53/85 (62%)
 Frame = +3

Query: 192 KKDQSRNREATQKGQTRCQKSLKLLLLGTGESGKSTFIRQMRIIHGSGYSDEDKRGFIKL 371
           K+ Q  +    Q+ + + Q+ ++LL L +G SGKSTFI+Q++I++  G++++++  +   
Sbjct: 21  KRSQHIDALLDQERERKSQE-IQLLTLVSGNSGKSTFIKQLKIVYDDGFNEKERLEYRPQ 79

Query: 372 VYQNIFMAMQSMIRAMDLLTIQYGN 446
           VY+ +   +  + RA++ L + + +
Sbjct: 80  VYRLLAGNIAKITRAVEQLGLHFNS 104


>SB_4844| Best HMM Match : G-alpha (HMM E-Value=3.6e-10)
          Length = 290

 Score = 46.0 bits (104), Expect = 2e-05
 Identities = 24/54 (44%), Positives = 37/54 (68%), Gaps = 1/54 (1%)
 Frame = +3

Query: 273 GTGESGKSTFIRQMRIIHG-SGYSDEDKRGFIKLVYQNIFMAMQSMIRAMDLLT 431
           G GESGKST ++QMRI+H   G+++ +KR  I+ + +NI  A+ ++  AM  LT
Sbjct: 156 GAGESGKSTIVKQMRILHDVHGFTELEKRQKIEDIKKNIRDAILTITGAMSTLT 209


>SB_58122| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 57

 Score = 34.3 bits (75), Expect = 0.069
 Identities = 13/52 (25%), Positives = 34/52 (65%)
 Frame = +3

Query: 291 KSTFIRQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAMDLLTIQYGN 446
           KSTFI+Q++I++  G++++++  +   VY+ +   +  + RA++ L + + +
Sbjct: 2   KSTFIKQLKIVYDDGFNEKERLEYRPQVYRLLAGNIAKITRAVEQLGLHFNS 53


>SB_11257| Best HMM Match : GCC2_GCC3 (HMM E-Value=2.7e-11)
          Length = 3810

 Score = 30.7 bits (66), Expect = 0.85
 Identities = 15/31 (48%), Positives = 18/31 (58%)
 Frame = -2

Query: 160  P*FFRAEGRNLSSRAEPCPFGRPFNRGTPLP 68
            P FF  EG+N  S   PCP G+   RG+P P
Sbjct: 2419 PGFFCPEGQN-KSNPYPCPVGKYCPRGSPSP 2448


>SB_23785| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 872

 Score = 30.3 bits (65), Expect = 1.1
 Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 7/57 (12%)
 Frame = +1

Query: 163 CCMSE---EAKEQKRINQEIERQLRKDKRDARRASNCSYLELESR----ANLLSSGR 312
           CC SE   E K +KR  +  E     +  D+R  S+  YLEL S+      L++SG+
Sbjct: 72  CCKSEGGTEDKHRKRFKRIHEVSHEPEHEDSRSGSDSGYLELTSKHLSHVKLVTSGK 128


>SB_6662| Best HMM Match : DUF755 (HMM E-Value=3.4)
          Length = 167

 Score = 30.3 bits (65), Expect = 1.1
 Identities = 20/72 (27%), Positives = 39/72 (54%), Gaps = 1/72 (1%)
 Frame = +1

Query: 178 EAKEQKRINQEIERQLRKDKRDARRASNCSYLE-LESRANLLSSGR*ESSTARVIATKTS 354
           E+++ + + +EIE+ L+  K+ A   + CS L+   S A   S+ R   ++ARV      
Sbjct: 22  ESEDLRLLREEIEKGLKDLKKVALLRNECSQLDWYGSNAGRCSNARVRPASARVRPASGR 81

Query: 355 AASSNSFTRTSS 390
           + +S+  +R +S
Sbjct: 82  STASSRRSRPAS 93


>SB_58528| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 399

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
 Frame = -2

Query: 331 EPWMILICLMKVDLPDSPVPS-RSSLRLFWHLVC 233
           E W+ L   ++V++PD P P   + LR++  L+C
Sbjct: 350 EGWVPLCAFLEVEVPDIPFPQINTGLRVYAELLC 383


>SB_5939| Best HMM Match : BAH (HMM E-Value=7.9e-18)
          Length = 1086

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 15/25 (60%), Positives = 17/25 (68%)
 Frame = +1

Query: 316 ESSTARVIATKTSAASSNSFTRTSS 390
           ESSTA   AT+T A+ SNS  R SS
Sbjct: 714 ESSTANSTATRTEASESNSADRQSS 738


>SB_5496| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1352

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 25/70 (35%), Positives = 36/70 (51%), Gaps = 1/70 (1%)
 Frame = +1

Query: 184  KEQKRINQEIERQLRKD-KRDARRASNCSYLELESRANLLSSGR*ESSTARVIATKTSAA 360
            K + + N  I    R++  R   +ASN S  + E+RAN L+SG   SS A   + KTS  
Sbjct: 1214 KLRAKRNLSIGNLFRQEPNRPGSQASNVSSDD-ENRANELASGISSSSRATQGSYKTSVT 1272

Query: 361  SSNSFTRTSS 390
            SS +  +  S
Sbjct: 1273 SSGNLLQNHS 1282


>SB_42044| Best HMM Match : ubiquitin (HMM E-Value=1.2e-06)
          Length = 1425

 Score = 28.7 bits (61), Expect = 3.4
 Identities = 19/67 (28%), Positives = 29/67 (43%), Gaps = 3/67 (4%)
 Frame = +3

Query: 60  YIFGSGVPRLKGLPKGQGSAREDRLRPSAL---KNHGVLHVRRSKGTKKDQSRNREATQK 230
           Y   S +P + G    Q     D  RPS++   KN+   + +R  G  ++ S  R+    
Sbjct: 619 YATPSSLP-VNGSSNSQDELDYDVPRPSSMVTDKNNDDTNSKRDSGVTRESSTERDRAGS 677

Query: 231 GQTRCQK 251
           G T C K
Sbjct: 678 GVTECAK 684


>SB_59642| Best HMM Match : DUF822 (HMM E-Value=5)
          Length = 595

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 14/49 (28%), Positives = 23/49 (46%)
 Frame = +2

Query: 392 GDAEYDTRHGPTNNTIWEPSNVEKAELISSIDFESVTTFRVRTWKLSRA 538
           G  + DT H      +WE    E+ E++ S+D  +  +F   T + S A
Sbjct: 437 GTLDQDTGHLSRTGEVWELGQSEEMEMLLSVDSSAHQSFSHYTGRYSLA 485


>SB_39571| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 317

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 15/52 (28%), Positives = 29/52 (55%)
 Frame = -1

Query: 395 RHEDVLVNEFDEAALVFVAITRAVDDSHLPDESRFARLSSSK*EQFEALLAS 240
           +HED +V+   +   +   +++A+ D H+ DE    RL   + E++ AL A+
Sbjct: 134 KHEDTMVSAESKKNSISELVSKALQDGHISDEE--FRLILQEQERYYALKAA 183


>SB_39561| Best HMM Match : ELMO_CED12 (HMM E-Value=2.52234e-44)
          Length = 520

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 13/37 (35%), Positives = 22/37 (59%)
 Frame = -2

Query: 511 SERCHTLKVDR*YQFRLFYIGRFPYCIVSRSMARIIL 401
           +ER HTL ++  Y+        FP+C++S ++ RI L
Sbjct: 368 NERTHTLALEM-YKLSQHEAQNFPFCVMSINITRIAL 403


>SB_30234| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 5222

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = -2

Query: 109 CPFGRPFNRGTPLPNIYP*GTIKCSTSN 26
           CP GR   +GT +P + P GT   +T N
Sbjct: 194 CPPGRYCEQGTKVPELCPQGTFSNNTGN 221


>SB_19357| Best HMM Match : Metallothio_5 (HMM E-Value=3.4)
          Length = 187

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 10/24 (41%), Positives = 19/24 (79%)
 Frame = +1

Query: 184 KEQKRINQEIERQLRKDKRDARRA 255
           +EQ+++NQ +E+ L+K +  +RRA
Sbjct: 80  REQRKVNQFVEKTLQKKEHTSRRA 103


>SB_37508| Best HMM Match : S-antigen (HMM E-Value=4.3)
          Length = 1347

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 14/49 (28%), Positives = 23/49 (46%)
 Frame = +2

Query: 392  GDAEYDTRHGPTNNTIWEPSNVEKAELISSIDFESVTTFRVRTWKLSRA 538
            G  + DT H      +WE    E+ E++ S+D  +  +F   T + S A
Sbjct: 1152 GTLDQDTGHLSRTGEVWELGQSEEMEMLLSVDSSAHQSFSHYTGRYSLA 1200


>SB_32678| Best HMM Match : HEAT (HMM E-Value=9.6e-18)
          Length = 844

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 15/43 (34%), Positives = 22/43 (51%)
 Frame = +3

Query: 171 VRRSKGTKKDQSRNREATQKGQTRCQKSLKLLLLGTGESGKST 299
           VR   G+K+D   NRE + +G        +    G+GESG +T
Sbjct: 696 VRYFAGSKEDWDENREYSTQGAHNSSVGRECRKEGSGESGDNT 738


>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
          Length = 640

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 16/47 (34%), Positives = 25/47 (53%)
 Frame = +3

Query: 147 LKNHGVLHVRRSKGTKKDQSRNREATQKGQTRCQKSLKLLLLGTGES 287
           L NH V   +R    KKD S+N  A ++ +T C+++ + L   T  S
Sbjct: 238 LVNHFVAEFKRKY--KKDMSKNSRAMRRLRTACERAKRTLSSSTEAS 282


>SB_3749| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 220

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 15/52 (28%), Positives = 29/52 (55%)
 Frame = -1

Query: 395 RHEDVLVNEFDEAALVFVAITRAVDDSHLPDESRFARLSSSK*EQFEALLAS 240
           +HED +V    +   +   +++A+ D H+ DE    RL + + E++ AL A+
Sbjct: 133 KHEDTMVLAESKKNSISELVSKALQDGHISDEE--FRLITQEQERYYALKAA 182


>SB_15458| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 662

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
 Frame = +1

Query: 175 EEAKEQKRINQEIERQLR--KDKRDARRASNCSYLEL 279
           E AKE KR+ +E+ +     KDK+D   A +  Y EL
Sbjct: 353 EHAKEMKRLEEELNKAKASLKDKQDQLLALDTKYKEL 389


>SB_50768| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1069

 Score = 27.5 bits (58), Expect = 7.9
 Identities = 15/41 (36%), Positives = 22/41 (53%)
 Frame = +3

Query: 114 SAREDRLRPSALKNHGVLHVRRSKGTKKDQSRNREATQKGQ 236
           SA  +   PS    HG   ++R+ GTK+DQS   +   +GQ
Sbjct: 20  SALTEGRSPSGRSRHGK-ELKRTSGTKRDQSIEGDYVIEGQ 59


>SB_27074| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 225

 Score = 27.5 bits (58), Expect = 7.9
 Identities = 16/52 (30%), Positives = 27/52 (51%)
 Frame = -1

Query: 395 RHEDVLVNEFDEAALVFVAITRAVDDSHLPDESRFARLSSSK*EQFEALLAS 240
           +HED +V        +   +++A+ D H+ DE    RL   K E++ AL A+
Sbjct: 133 KHEDTMVLAESNKNSISELVSKALQDGHISDEE--FRLILRKQERYYALKAA 182


>SB_540| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 253

 Score = 27.5 bits (58), Expect = 7.9
 Identities = 20/70 (28%), Positives = 37/70 (52%), Gaps = 4/70 (5%)
 Frame = +1

Query: 103 KDRAPRERTDCGPPL*KIMECCMSEEAKEQKRINQEIER----QLRKDKRDARRASNCSY 270
           +DR+ R++ D      +  +    +E+KE++R ++E E     +  KDK ++   S+ S 
Sbjct: 102 RDRSKRDKKDRKEKRDRRSKERSKDESKEERRRSKERENSPDAKKGKDKENSDSGSSDSE 161

Query: 271 LELESRANLL 300
            ELE+R   L
Sbjct: 162 TELENRRRAL 171


>SB_24713| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 206

 Score = 27.5 bits (58), Expect = 7.9
 Identities = 10/39 (25%), Positives = 22/39 (56%)
 Frame = -1

Query: 416 GAYHTLHRHEDVLVNEFDEAALVFVAITRAVDDSHLPDE 300
           G+Y  + +HED +V    +  L+   +++A+ D  + D+
Sbjct: 107 GSYEQVAKHEDTMVLAESKKNLISELVSKALQDGRISDD 145


>SB_21990| Best HMM Match : DUF828 (HMM E-Value=1.4)
          Length = 415

 Score = 27.5 bits (58), Expect = 7.9
 Identities = 15/52 (28%), Positives = 28/52 (53%)
 Frame = -1

Query: 395 RHEDVLVNEFDEAALVFVAITRAVDDSHLPDESRFARLSSSK*EQFEALLAS 240
           +HED +V    +   +   +++A+ D H+ DE    RL   + E++ AL A+
Sbjct: 339 KHEDTMVLAESKKNSISELVSKALQDGHISDEE--FRLILQEQERYNALKAA 388


>SB_3271| Best HMM Match : PTPS (HMM E-Value=6.6)
          Length = 191

 Score = 27.5 bits (58), Expect = 7.9
 Identities = 19/52 (36%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
 Frame = +2

Query: 407 DTRHGPTNNTIWEPSNVEKAELISSIDFESVTTFRVRTW-KLSRAYGPIPAS 559
           DT  GP NNTI + S  E  E + ++ F+      +R W   S A GP+  S
Sbjct: 128 DTLDGPRNNTIRDESPSEVNE-VFALFFKLQVQIILREWTHNSPAVGPLEVS 178


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,130,359
Number of Sequences: 59808
Number of extensions: 392697
Number of successful extensions: 1341
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 1211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1340
length of database: 16,821,457
effective HSP length: 78
effective length of database: 12,156,433
effective search space used: 1312894764
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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