Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0434
(560 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26300.1 68415.m03156 guanine nucleotide binding protein (G-p... 50 8e-07
At1g31930.2 68414.m03924 extra-large guanine nucleotide binding ... 37 0.008
At1g31930.1 68414.m03923 extra-large guanine nucleotide binding ... 37 0.008
At4g34390.1 68417.m04885 extra-large guanine nucleotide binding ... 34 0.057
At3g13080.4 68416.m01638 ABC transporter family protein almost i... 29 1.6
At3g13080.3 68416.m01637 ABC transporter family protein almost i... 29 1.6
At3g13080.2 68416.m01636 ABC transporter family protein almost i... 29 1.6
At3g13080.1 68416.m01635 ABC transporter family protein almost i... 29 1.6
At4g12780.1 68417.m02005 auxilin-related low similarity to SP|Q2... 29 2.8
At3g28925.1 68416.m03612 hypothetical protein 28 3.7
At2g39580.1 68415.m04855 expressed protein 28 4.9
At5g55190.1 68418.m06880 Ras-related GTP-binding protein (RAN3) ... 27 6.5
At5g20020.1 68418.m02382 Ras-related GTP-binding nuclear protein... 27 6.5
At5g20010.1 68418.m02381 Ras-related GTP-binding nuclear protein... 27 6.5
At4g12770.1 68417.m02004 auxilin-related low similarity to SP|Q2... 27 6.5
At5g49110.1 68418.m06079 expressed protein ; expression support... 27 8.6
At3g24390.1 68416.m03063 Ulp1 protease family protein contains P... 27 8.6
At2g07672.1 68415.m00897 hypothetical protein 27 8.6
At1g32840.1 68414.m04047 Ulp1 protease family protein similar to... 27 8.6
At1g32415.1 68414.m04001 pentatricopeptide (PPR) repeat-containi... 27 8.6
At1g09180.1 68414.m01025 GTP-binding protein, putative strong si... 27 8.6
>At2g26300.1 68415.m03156 guanine nucleotide binding protein
(G-protein) alpha-1 subunit / GP-alpha-1 (GPA1)
identical to SP|P18064 Guanine nucleotide-binding
protein alpha-1 subunit (GP-alpha-1) {Arabidopsis
thaliana}
Length = 383
Score = 50.4 bits (115), Expect = 8e-07
Identities = 22/81 (27%), Positives = 50/81 (61%), Gaps = 6/81 (7%)
Frame = +3
Query: 177 RSKGTKKDQSRNREATQ-----KGQTRCQKSL-KLLLLGTGESGKSTFIRQMRIIHGSGY 338
RS+ +D N +A + + + + +K + KLLLLG GESGKST +Q++++ +G+
Sbjct: 7 RSRHHTEDTDENTQAAEIERRIEQEAKAEKHIRKLLLLGAGESGKSTIFKQIKLLFQTGF 66
Query: 339 SDEDKRGFIKLVYQNIFMAMQ 401
+ + + ++ +++ N++ ++
Sbjct: 67 DEGELKSYVPVIHANVYQTIK 87
>At1g31930.2 68414.m03924 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 848
Score = 37.1 bits (82), Expect = 0.008
Identities = 20/70 (28%), Positives = 36/70 (51%)
Frame = +3
Query: 201 QSRNREATQKGQTRCQKSLKLLLLGTGESGKSTFIRQMRIIHGSGYSDEDKRGFIKLVYQ 380
Q + AT +K KLLLLG SG ST +Q + ++G+ +S E+ + +V
Sbjct: 413 QPSSNYATVPNYIEHKKIQKLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQS 472
Query: 381 NIFMAMQSMI 410
N++ + ++
Sbjct: 473 NMYRYLSILL 482
>At1g31930.1 68414.m03923 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 848
Score = 37.1 bits (82), Expect = 0.008
Identities = 20/70 (28%), Positives = 36/70 (51%)
Frame = +3
Query: 201 QSRNREATQKGQTRCQKSLKLLLLGTGESGKSTFIRQMRIIHGSGYSDEDKRGFIKLVYQ 380
Q + AT +K KLLLLG SG ST +Q + ++G+ +S E+ + +V
Sbjct: 413 QPSSNYATVPNYIEHKKIQKLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQS 472
Query: 381 NIFMAMQSMI 410
N++ + ++
Sbjct: 473 NMYRYLSILL 482
>At4g34390.1 68417.m04885 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 861
Score = 34.3 bits (75), Expect = 0.057
Identities = 18/68 (26%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = +3
Query: 246 QKSL-KLLLLGTGESGKSTFIRQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAMD 422
QK L KLLL+G+ + G +T +Q R ++ +S ED+ ++ N++ + ++ A +
Sbjct: 459 QKMLNKLLLIGSEKGGATTIYKQARSLYNVSFSLEDRERIKFIIQTNLYTYLAMVLEAHE 518
Query: 423 LLTIQYGN 446
+ N
Sbjct: 519 RFEKEMSN 526
>At3g13080.4 68416.m01638 ABC transporter family protein almost
identical to MRP-like ABC transporter GI:2316016 from
[Arabidopsis thaliana]; contains Pfam profile: PF00005
ABC transporter
Length = 1120
Score = 29.5 bits (63), Expect = 1.6
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +2
Query: 101 ERTGLRARGQIAALRSEKSWSAACQKKQRNKKGSI 205
++ G+R R + A+ EK + +CQ KQ G I
Sbjct: 389 QKVGIRMRSALVAMIYEKGLTLSCQSKQGRTSGEI 423
>At3g13080.3 68416.m01637 ABC transporter family protein almost
identical to MRP-like ABC transporter GI:2316016 from
[Arabidopsis thaliana]; contains Pfam profile: PF00005
ABC transporter
Length = 1120
Score = 29.5 bits (63), Expect = 1.6
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +2
Query: 101 ERTGLRARGQIAALRSEKSWSAACQKKQRNKKGSI 205
++ G+R R + A+ EK + +CQ KQ G I
Sbjct: 389 QKVGIRMRSALVAMIYEKGLTLSCQSKQGRTSGEI 423
>At3g13080.2 68416.m01636 ABC transporter family protein almost
identical to MRP-like ABC transporter GI:2316016 from
[Arabidopsis thaliana]; contains Pfam profile: PF00005
ABC transporter
Length = 1489
Score = 29.5 bits (63), Expect = 1.6
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +2
Query: 101 ERTGLRARGQIAALRSEKSWSAACQKKQRNKKGSI 205
++ G+R R + A+ EK + +CQ KQ G I
Sbjct: 389 QKVGIRMRSALVAMIYEKGLTLSCQSKQGRTSGEI 423
>At3g13080.1 68416.m01635 ABC transporter family protein almost
identical to MRP-like ABC transporter GI:2316016 from
[Arabidopsis thaliana]; contains Pfam profile: PF00005
ABC transporter
Length = 1514
Score = 29.5 bits (63), Expect = 1.6
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +2
Query: 101 ERTGLRARGQIAALRSEKSWSAACQKKQRNKKGSI 205
++ G+R R + A+ EK + +CQ KQ G I
Sbjct: 389 QKVGIRMRSALVAMIYEKGLTLSCQSKQGRTSGEI 423
>At4g12780.1 68417.m02005 auxilin-related low similarity to
SP|Q27974 Auxilin {Bos taurus}
Length = 485
Score = 28.7 bits (61), Expect = 2.8
Identities = 24/93 (25%), Positives = 43/93 (46%), Gaps = 2/93 (2%)
Frame = +1
Query: 109 RAPRERTDCGPPL*KIMECCMSEEAKEQKRINQEIERQLRKD--KRDARRASNCSYLELE 282
R RER + + K E E +EQKRI +E ER + + +R R A + E
Sbjct: 91 RLDRERAEREAEMEKAQEREKEEREREQKRIERERERLVARQAVERATREARERAATEAH 150
Query: 283 SRANLLSSGR*ESSTARVIATKTSAASSNSFTR 381
++ + G+ ++ AR A + + +++ R
Sbjct: 151 AKVQRAAVGK--ATDARERAERAAVQRAHAEAR 181
>At3g28925.1 68416.m03612 hypothetical protein
Length = 247
Score = 28.3 bits (60), Expect = 3.7
Identities = 17/50 (34%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = +3
Query: 147 LKNHGVLHVRRSKGTKKDQSRNREATQKGQTRCQKSL-KLLLLGTGESGK 293
LK +HV+ K +++ RNRE + KG+ C L + + + ESGK
Sbjct: 22 LKELESVHVKHKK--RQEMLRNRELSDKGRKSCSNHLYESMKQRSSESGK 69
>At2g39580.1 68415.m04855 expressed protein
Length = 1567
Score = 27.9 bits (59), Expect = 4.9
Identities = 18/47 (38%), Positives = 28/47 (59%), Gaps = 3/47 (6%)
Frame = +1
Query: 175 EEAKEQKRINQEIERQLRKDKRDARRA---SNCSYLELESRANLLSS 306
EEA+E+KR+ + ER K R A+R+ +N EL S+ +LS+
Sbjct: 435 EEAQERKRLFEIEERNALKVYRKAQRSLIEANARCAELYSKREILSA 481
>At5g55190.1 68418.m06880 Ras-related GTP-binding protein (RAN3)
identical to atran3 [Arabidopsis thaliana] GI:2058280
Length = 221
Score = 27.5 bits (58), Expect = 6.5
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = +3
Query: 234 QTRCQKSLKLLLLGTGESGKSTFIRQ 311
QT S KL+++G G +GK+TF+++
Sbjct: 7 QTVDYPSFKLVIVGDGGTGKTTFVKR 32
>At5g20020.1 68418.m02382 Ras-related GTP-binding nuclear protein
(RAN-2) identical to GTP-binding nuclear protein RAN-2
SP:P41917 from [Arabidopsis thaliana]
Length = 221
Score = 27.5 bits (58), Expect = 6.5
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = +3
Query: 234 QTRCQKSLKLLLLGTGESGKSTFIRQ 311
QT S KL+++G G +GK+TF+++
Sbjct: 7 QTVDYPSFKLVIVGDGGTGKTTFVKR 32
>At5g20010.1 68418.m02381 Ras-related GTP-binding nuclear protein
(RAN-1) identical to GTP-binding nuclear protein RAN-1
SP:P41916 from [Arabidopsis thaliana]
Length = 221
Score = 27.5 bits (58), Expect = 6.5
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = +3
Query: 234 QTRCQKSLKLLLLGTGESGKSTFIRQ 311
QT S KL+++G G +GK+TF+++
Sbjct: 7 QTVDYPSFKLVIVGDGGTGKTTFVKR 32
>At4g12770.1 68417.m02004 auxilin-related low similarity to
SP|Q27974 Auxilin {Bos taurus}
Length = 909
Score = 27.5 bits (58), Expect = 6.5
Identities = 23/96 (23%), Positives = 39/96 (40%), Gaps = 2/96 (2%)
Frame = +1
Query: 109 RAPRERTDCGPPL*KIMECCMSEEAKEQKRINQEIERQLRKD--KRDARRASNCSYLELE 282
R RER + + K E +EQKRI +E ER L + +R R A + E
Sbjct: 508 RLDRERAEREAEMEKTQAREREEREREQKRIERERERLLARQAVERATREARERAATEAH 567
Query: 283 SRANLLSSGR*ESSTARVIATKTSAASSNSFTRTSS 390
++ + G+ + R A + + R ++
Sbjct: 568 AKVQRAAVGKVTDARERAERAAVQRAHAEARERAAA 603
>At5g49110.1 68418.m06079 expressed protein ; expression supported by
MPSS
Length = 1487
Score = 27.1 bits (57), Expect = 8.6
Identities = 21/77 (27%), Positives = 35/77 (45%), Gaps = 6/77 (7%)
Frame = +1
Query: 97 CRKDRAPRERTDCG------PPL*KIMECCMSEEAKEQKRINQEIERQLRKDKRDARRAS 258
C K+ P + CG PPL K++ E + N+E ++ RKD ++
Sbjct: 908 CMKEENPLKPLVCGDMKVLGPPLLKVVYLLKPEPPLATGQTNKE--KKGRKDVDGRKQCL 965
Query: 259 NCSYLELESRANLLSSG 309
+ + L L+ N+ SSG
Sbjct: 966 HLALLSLKELLNIYSSG 982
>At3g24390.1 68416.m03063 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At3g24380, At5g36840,
At5g35010, At3g42740, At4g05290, At2g14770, At3g43390,
At2g05560, At4g08880, At1g34730, At1g27790, At1g34740,
At1g27780, At5g36850, At3g42730, At1g52020, At4g05280,
At1g25886, At4g03300
Length = 1139
Score = 27.1 bits (57), Expect = 8.6
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +2
Query: 380 EHLHGDAEYDTRHGPTNNTIWEPSNVEKAELISSIDFESV 499
E + ++ DT H P + IWE +L ++ D E V
Sbjct: 734 EETYEPSDGDTAHVPATSVIWELDTKAVCDLAAATDLEVV 773
>At2g07672.1 68415.m00897 hypothetical protein
Length = 159
Score = 27.1 bits (57), Expect = 8.6
Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 2/34 (5%)
Frame = -2
Query: 151 FRAEGRNLSSRAEPCPFGRPFNRGTPLP--NIYP 56
F + R +S EP P+ F+R PL +IYP
Sbjct: 103 FHPDSRKMSCHYEPLPYQTRFDRQAPLQEYSIYP 136
>At1g32840.1 68414.m04047 Ulp1 protease family protein similar to
At4g04010, At2g06430, At2g15140, At2g04980, At2g14130,
At3g44500, At2g15190, At3g47260, At5g34900, At3g29210,
At2g02210, At3g32900
Length = 611
Score = 27.1 bits (57), Expect = 8.6
Identities = 13/32 (40%), Positives = 22/32 (68%), Gaps = 2/32 (6%)
Frame = +1
Query: 172 SEEAKEQKRINQEIERQLRKDKRDA--RRASN 261
SEE +E+K+ ++E+ERQL ++ R+A N
Sbjct: 179 SEEDEERKQEHEEVERQLEAEEEGGLERKAEN 210
>At1g32415.1 68414.m04001 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 761
Score = 27.1 bits (57), Expect = 8.6
Identities = 12/43 (27%), Positives = 26/43 (60%), Gaps = 2/43 (4%)
Frame = +1
Query: 208 EIERQLRKDK--RDARRASNCSYLELESRANLLSSGR*ESSTA 330
++E+++RK+ + ++ CS++ + RAN+ SG +S A
Sbjct: 692 DMEKEMRKEMGIKGVKKTPGCSWVVVNGRANVFLSGDKSASEA 734
>At1g09180.1 68414.m01025 GTP-binding protein, putative strong
similarity to SP:Q01474 GTP-binding protein SAR1B and
SP:O04834 GTP-binding protein SAR1A [Arabidopsis
thaliana]
Length = 193
Score = 27.1 bits (57), Expect = 8.6
Identities = 9/25 (36%), Positives = 16/25 (64%)
Frame = +3
Query: 243 CQKSLKLLLLGTGESGKSTFIRQMR 317
C+K K+L LG +GK+T + ++
Sbjct: 17 CKKEAKILFLGLDNAGKTTLLHMLK 41
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,645,850
Number of Sequences: 28952
Number of extensions: 266582
Number of successful extensions: 932
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 912
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 932
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1072696904
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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