BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0417 (765 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g10572.1 68416.m01269 3-phosphoinositide-dependent protein ki... 32 0.36 At1g11570.1 68414.m01328 nuclear transport factor 2 (NTF2), puta... 30 1.9 At1g08135.1 68414.m00894 cation/hydrogen exchanger (CHX6b) Note:... 28 5.9 At5g05450.1 68418.m00587 DEAD/DEAH box helicase, putative (RH18) 28 7.8 At1g26799.1 68414.m03265 expressed protein 28 7.8 >At3g10572.1 68416.m01269 3-phosphoinositide-dependent protein kinase-1, putative annotation temporarily based on supporting cDNA gi|17065215|gb|AY062684.1| Length = 333 Score = 32.3 bits (70), Expect = 0.36 Identities = 19/60 (31%), Positives = 31/60 (51%) Frame = -1 Query: 615 KLFFSKKNIIVTYKLKRKIAY*ISTKNKQIKVSTKKIPHFVTVTFSFSYETNLTIKTIPN 436 K+ S +I+ Y LKRK A I +Q++ + K I F + FS+ I++IP+ Sbjct: 271 KVAVSLVGLIICYALKRKRAALIRIIRRQMESTRKAIVDFWKLAFSYQVNPLAAIQSIPS 330 >At1g11570.1 68414.m01328 nuclear transport factor 2 (NTF2), putative similar to Swiss-Prot:P33331 nuclear transport factor 2 (NTF-2) (Nuclear transport factor P10) [Saccharomyces cerevisiae] Length = 127 Score = 29.9 bits (64), Expect = 1.9 Identities = 13/25 (52%), Positives = 17/25 (68%) Frame = +1 Query: 64 ITHYITLFFEVRNILSSLFNPISLI 138 + HY LF R+ LSSL+NP SL+ Sbjct: 17 VNHYYHLFDNDRSSLSSLYNPTSLL 41 >At1g08135.1 68414.m00894 cation/hydrogen exchanger (CHX6b) Note: CHX6a and CHX6b were originally 1 gene but were split pased on alignments with other family members; may be a pseudogene and requires futher investigation; monovalent cation:proton antiporter family 2 (CPA2) member, PMID:11500563 Length = 824 Score = 28.3 bits (60), Expect = 5.9 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +1 Query: 61 NITHYITLFFEVRNILSSLFNPISLILRSH 150 NITH++ LF +V +LS +F + + H Sbjct: 114 NITHFLFLFLKVGAVLSEMFGSMQIPCLKH 143 >At5g05450.1 68418.m00587 DEAD/DEAH box helicase, putative (RH18) Length = 593 Score = 27.9 bits (59), Expect = 7.8 Identities = 8/13 (61%), Positives = 11/13 (84%) Frame = -2 Query: 272 FIRVWKRHHCSFV 234 F+R +K HHCSF+ Sbjct: 437 FVRAYKEHHCSFI 449 >At1g26799.1 68414.m03265 expressed protein Length = 151 Score = 27.9 bits (59), Expect = 7.8 Identities = 15/59 (25%), Positives = 28/59 (47%) Frame = -1 Query: 612 LFFSKKNIIVTYKLKRKIAY*ISTKNKQIKVSTKKIPHFVTVTFSFSYETNLTIKTIPN 436 L FS K++++ L + + NK+ + +K+P + +F F + NL T N Sbjct: 36 LLFSPKHVVIYNTLTSRATLVVHCVNKEKDLGIQKLP--IGASFDFRFRVNLRKTTTYN 92 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,922,889 Number of Sequences: 28952 Number of extensions: 257386 Number of successful extensions: 516 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 510 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 516 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1712086600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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