Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0416
(765 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12890.1 68417.m02017 gamma interferon responsive lysosomal t... 56 2e-08
At4g12900.1 68417.m02018 gamma interferon responsive lysosomal t... 51 1e-06
At4g12870.1 68417.m02015 gamma interferon responsive lysosomal t... 50 1e-06
At4g12960.1 68417.m02025 gamma interferon responsive lysosomal t... 48 7e-06
At1g07080.1 68414.m00754 gamma interferon responsive lysosomal t... 47 1e-05
At5g01580.1 68418.m00073 gamma interferon responsive lysosomal t... 46 2e-05
At1g47340.1 68414.m05241 F-box family protein contains F-box dom... 30 1.9
At4g00416.1 68417.m00056 methyl-CpG-binding domain-containing pr... 29 3.4
At1g22770.1 68414.m02845 gigantea protein (GI) identical to giga... 29 4.5
>At4g12890.1 68417.m02017 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 232
Score = 56.4 bits (130), Expect = 2e-08
Identities = 30/99 (30%), Positives = 49/99 (49%), Gaps = 6/99 (6%)
Frame = +1
Query: 463 FCQKFSTGDR-KAF-----RVLDVTLVPYGKATTKEINGKYIFSCQHGEEECYANKIHSC 624
+CQ F D K F ++ D+ LVP+G A I+ +CQHGEEEC N + +C
Sbjct: 51 YCQNFIVDDLGKIFDSDLLKITDLKLVPFGNA---HISNNLTITCQHGEEECKLNALEAC 107
Query: 625 SIEAVTNMTKAVKFTTCMITDNNDADEALQRCSKSMNID 741
I + + KF C+ D N+ + +++ + I+
Sbjct: 108 GIRTLPDPKLQYKFIRCVEKDTNEWESCVKKSGREKAIN 146
Score = 32.3 bits (70), Expect = 0.36
Identities = 12/27 (44%), Positives = 19/27 (70%)
Frame = +2
Query: 407 DKVKVRVYYEALCPDSKYFFVKNLAPV 487
+KVK+ +YYE+LCP + F V +L +
Sbjct: 37 NKVKINLYYESLCPYCQNFIVDDLGKI 63
>At4g12900.1 68417.m02018 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 231
Score = 50.8 bits (116), Expect = 1e-06
Identities = 22/71 (30%), Positives = 38/71 (53%)
Frame = +1
Query: 505 VLDVTLVPYGKATTKEINGKYIFSCQHGEEECYANKIHSCSIEAVTNMTKAVKFTTCMIT 684
+ D+ L+P+G A ++ +CQHGEEEC N + +C+I N KF C+ T
Sbjct: 69 ITDLKLIPFGNA---HVSDDLTVTCQHGEEECKLNALEACAIRTWPNQRLHYKFIRCVET 125
Query: 685 DNNDADEALQR 717
+ N + +++
Sbjct: 126 NTNAWESCVKK 136
Score = 35.1 bits (77), Expect = 0.052
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = +2
Query: 401 KQDKVKVRVYYEALCPDSKYFFVKNLAPV 487
+ DKVK+ +YYE+LCP + F V +L +
Sbjct: 33 ESDKVKLNLYYESLCPSCQNFIVHHLGKI 61
>At4g12870.1 68417.m02015 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 229
Score = 50.4 bits (115), Expect = 1e-06
Identities = 28/86 (32%), Positives = 41/86 (47%)
Frame = +1
Query: 505 VLDVTLVPYGKATTKEINGKYIFSCQHGEEECYANKIHSCSIEAVTNMTKAVKFTTCMIT 684
+ DV LVP+G A +++ CQHGEEEC N + +C I + N KF C+
Sbjct: 65 ITDVKLVPFGYA---KVSNNLTVICQHGEEECKLNALEACVINTLPNPKSQYKFIRCV-- 119
Query: 685 DNNDADEALQRCSKSMNIDSDPISNC 762
N+ D C K + I++C
Sbjct: 120 -ENNTDNWESSCLKGYG-NEKAINDC 143
Score = 33.1 bits (72), Expect = 0.21
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = +2
Query: 401 KQDKVKVRVYYEALCPDSKYFFVKNLAPV 487
+ DKV++ +YYE+LCP + F V L V
Sbjct: 29 ESDKVELNLYYESLCPGCQSFIVDELVKV 57
>At4g12960.1 68417.m02025 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 48.0 bits (109), Expect = 7e-06
Identities = 21/85 (24%), Positives = 40/85 (47%)
Frame = +1
Query: 487 DRKAFRVLDVTLVPYGKATTKEINGKYIFSCQHGEEECYANKIHSCSIEAVTNMTKAVKF 666
D + + D+ L P+G A E++ +CQHGEEEC N + +C++ + F
Sbjct: 58 DYDLYTITDLKLFPFGNA---ELSDNLTVTCQHGEEECKLNALEACALRTWPDQKSQYSF 114
Query: 667 TTCMITDNNDADEALQRCSKSMNID 741
C+ +D + ++ + I+
Sbjct: 115 IRCVESDTKGWESCVKNSGREKAIN 139
Score = 33.5 bits (73), Expect = 0.16
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = +2
Query: 401 KQDKVKVRVYYEALCPDSKYFFVKNLAPV 487
K KVK+ +YYE+LCP + F V +L +
Sbjct: 28 KSGKVKLNLYYESLCPGCQEFIVDDLGKI 56
>At1g07080.1 68414.m00754 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 265
Score = 47.2 bits (107), Expect = 1e-05
Identities = 22/89 (24%), Positives = 49/89 (55%), Gaps = 3/89 (3%)
Frame = +1
Query: 505 VLDVTLVPYGKATTKEINGKYIFSCQHGEEECYANKIHSCSIEAVTNMTKAVKFTTC--- 675
++D+ L P+G + N + CQHG EC+ + + +C+I+A ++ F C
Sbjct: 71 IVDLHLSPWGNTKLRSDNVTAV--CQHGAFECFLDTVEACAIDAWPKVSDHFPFIYCVEK 128
Query: 676 MITDNNDADEALQRCSKSMNIDSDPISNC 762
++T++ + + C + +N++S P+++C
Sbjct: 129 LVTEHK--YDKWETCYEKLNLNSKPVADC 155
Score = 31.5 bits (68), Expect = 0.64
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +2
Query: 410 KVKVRVYYEALCPDSKYFFVKNLAPVTE 493
KV V +YYE+LCP F V +LA + E
Sbjct: 38 KVSVGLYYESLCPYCSSFIVNHLAKLFE 65
>At5g01580.1 68418.m00073 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 46.4 bits (105), Expect = 2e-05
Identities = 22/56 (39%), Positives = 33/56 (58%)
Frame = +1
Query: 508 LDVTLVPYGKATTKEINGKYIFSCQHGEEECYANKIHSCSIEAVTNMTKAVKFTTC 675
+D+ LVP+G A + +G + CQHGE EC N IH+C+I A ++ K + C
Sbjct: 60 IDLQLVPWGNAAIRP-DGTIL--CQHGEAECALNAIHACAINAYPDVMKHFGYIYC 112
Score = 29.5 bits (63), Expect = 2.6
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +2
Query: 410 KVKVRVYYEALCPDSKYFFVKNLAPVTE 493
KV + +YYEALCP F V L + E
Sbjct: 26 KVTLSLYYEALCPFCAEFIVNRLPKIFE 53
>At1g47340.1 68414.m05241 F-box family protein contains F-box domain
Pfam:PF00646
Length = 459
Score = 29.9 bits (64), Expect = 1.9
Identities = 13/30 (43%), Positives = 21/30 (70%)
Frame = -2
Query: 230 SLKSFEHFIKLFLSRSDHSQPLILSILKKY 141
S+ S HF +LFL+RS +QP +L ++K+
Sbjct: 66 SMLSCPHFRELFLTRSSSAQPRLLFAIEKH 95
>At4g00416.1 68417.m00056 methyl-CpG-binding domain-containing
protein contains Pfam profile PF01429: Methyl-CpG
binding domain
Length = 163
Score = 29.1 bits (62), Expect = 3.4
Identities = 16/33 (48%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Frame = +2
Query: 413 VKVRVYYEALCPDSKYF-FVKNLAPVTEKLSEF 508
VKV VYYE+L P K F +K +A E EF
Sbjct: 90 VKVDVYYESLAPRRKRFKSIKEVATFIEDKEEF 122
>At1g22770.1 68414.m02845 gigantea protein (GI) identical to
gigantea protein SP:Q9SQI2 from [Arabidopsis thaliana]
Length = 1173
Score = 28.7 bits (61), Expect = 4.5
Identities = 20/76 (26%), Positives = 40/76 (52%)
Frame = -2
Query: 653 LVILVTASIEQECILLA*HSSSPC*HENMYFPLISLVVALP*GTKVTSRTLKAFLSPVLN 474
L+ L +++++ I++A P N+Y PL+ ++ LP G+ + +K F++ V
Sbjct: 472 LIPLNSSAVDLPEIIVATPLQPPILSWNLYIPLLKVLEYLPRGSPSEACLMKIFVATV-- 529
Query: 473 F*QKNILSQDIEPHST 426
+ ILS+ P S+
Sbjct: 530 ---ETILSRTFPPESS 542
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,847,455
Number of Sequences: 28952
Number of extensions: 253625
Number of successful extensions: 652
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 633
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 652
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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