Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0409
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone... 34 0.025
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 30 0.40
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 28 1.2
SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 28 1.2
SPBC83.16c |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.6
SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 27 3.8
SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma... 26 5.0
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 26 5.0
SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc... 26 5.0
SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosacchar... 26 6.6
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 26 6.6
SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces ... 25 8.7
>SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 809
Score = 33.9 bits (74), Expect = 0.025
Identities = 19/55 (34%), Positives = 36/55 (65%), Gaps = 3/55 (5%)
Frame = +1
Query: 253 RYE---LLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVN 408
RYE +LE D S+L+ L+R+N+++ +QLK + NI++++++ E E+N
Sbjct: 635 RYEHPKVLEDN-DSTISSLESLKRENEELREQLKVEHEENISMQKQLSELKGELN 688
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 29.9 bits (64), Expect = 0.40
Identities = 12/63 (19%), Positives = 33/63 (52%)
Frame = +1
Query: 244 YNRRYELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNNSN*R 423
Y + + +++++E+ + L+ + + + Q+K LL+ N ++++ + + N
Sbjct: 306 YEEKLSIAQNSINEIQTQNRDLKLETEKLQDQIKALLERNQSLQEALETVKNDEKNLREM 365
Query: 424 NAN 432
NAN
Sbjct: 366 NAN 368
Score = 28.7 bits (61), Expect = 0.94
Identities = 14/44 (31%), Positives = 26/44 (59%)
Frame = +1
Query: 286 LNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNNSN 417
L S + E + +H L+ L Q+N +++ E+ E+N E+N+ N
Sbjct: 520 LKSQIRDQELTIEKLHDSLETLSQTNNSLQCEISEKNAELNSVN 563
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 28.3 bits (60), Expect = 1.2
Identities = 14/55 (25%), Positives = 28/55 (50%)
Frame = +1
Query: 247 NRRYELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNN 411
N + E +DELNSA L ++ ++ +L + N +++++ EVN+
Sbjct: 491 NAKLEKANIVIDELNSAKLKLSKQVEEESSMKDDLTEMNQRLKEQIESYENEVNS 545
>SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit
Rpt5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 438
Score = 28.3 bits (60), Expect = 1.2
Identities = 12/52 (23%), Positives = 28/52 (53%)
Frame = +1
Query: 259 ELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNNS 414
E+L + +DELNS LE + + + L + ++++E ++++N+
Sbjct: 30 EILAAGIDELNSRTRLLENDIKVMKSEFQRLTHEKSTMLEKIKENQEKISNN 81
>SPBC83.16c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 563
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/50 (24%), Positives = 27/50 (54%)
Frame = +2
Query: 311 SVKMMIFTNN*KSFCNPILQLDKKCVKKIRKSTTVIEEMQIQHYFKFNCA 460
S+ +M+ + FC+ + K V K+RK+ ++ ++ +H+ F+ A
Sbjct: 103 SIGLMLLLSALIGFCSESIVTSVKSVYKLRKAHSIFSKINKRHFDHFSAA 152
>SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 161
Score = 26.6 bits (56), Expect = 3.8
Identities = 10/35 (28%), Positives = 22/35 (62%)
Frame = +1
Query: 307 LERKNDDIHQQLKELLQSNIAIRQEMREENKEVNN 411
LER+ DD+ Q+L+E+ ++ E+ E ++ + +
Sbjct: 126 LERERDDMEQKLEEMTDKYTKVKAELDEVHQALED 160
>SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr
2|||Manual
Length = 918
Score = 26.2 bits (55), Expect = 5.0
Identities = 19/92 (20%), Positives = 40/92 (43%)
Frame = -3
Query: 484 ITLGNMYICTIKFKIVLYLHFFNYCC*LPYFLHAFLV*LQYWIAKALSIVGEYHHFYAPR 305
I L M + +I ++L LH L L + + + Y+ SI HH +A
Sbjct: 618 IVLALMALSSIILPLILQLHLRRV---LGLSLKLYHLSILYFFELISSIFWIAHHVFAND 674
Query: 304 NPMQN*VHLM*ILIIHTFCYIFIVIIFWRFYV 209
++ H + + +T+ + ++ W+F++
Sbjct: 675 ALLEKQYHHVLYSLANTYVICILGVLIWQFFL 706
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 26.2 bits (55), Expect = 5.0
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = +1
Query: 265 LESTLDELNSALDFLERKNDDIHQQLKE 348
L T ELNSALD +++ +IH +LKE
Sbjct: 1346 LVDTRKELNSALDSCKKREAEIH-RLKE 1372
>SPAC1A6.09c |lag1||sphingosine N-acyltransferase
Lag1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/55 (21%), Positives = 27/55 (49%), Gaps = 2/55 (3%)
Frame = +1
Query: 529 YHLSIFSDQYHKVSVSF--K*CIIIHLRYLSMNNYLSCLHHLNVISCIFMSIGMN 687
Y++S Y+ + + F + +++HL +++ HH+ + I +S G N
Sbjct: 195 YYMSPLFKAYYLIQLGFWLQQILVLHLEQRRADHWQMFAHHIVTCALIILSYGFN 249
>SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 598
Score = 25.8 bits (54), Expect = 6.6
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = +1
Query: 310 ERKNDDIHQQLKELLQSNIAIRQEMREENKEVNNSN 417
+R+N Q +++L +SN+ + + E K +NSN
Sbjct: 550 KRRNSGTEQVVRQLSKSNVEVIGKGGERKKVASNSN 585
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/44 (29%), Positives = 24/44 (54%)
Frame = +1
Query: 250 RRYELLESTLDELNSALDFLERKNDDIHQQLKELLQSNIAIRQE 381
R EL +S + + A + ++ H+Q K L++S I+ R+E
Sbjct: 960 RMAELEKSNIQQKYLASEKTLEMMNETHEQFKHLVESEISTREE 1003
>SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 511
Score = 25.4 bits (53), Expect = 8.7
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +1
Query: 286 LNSALDFLERKNDDIHQQLKELLQSNIAIRQEMREENKEVNN 411
+++ L+ LE DD + +LKE NIA +QE+ E+++
Sbjct: 433 VDAYLNPLEFNFDDKNVKLKEFGSPNIAYKQELNSFQGELSS 474
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,664,362
Number of Sequences: 5004
Number of extensions: 49116
Number of successful extensions: 152
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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