Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0386
(743 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 51 7e-07
At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 50 1e-06
At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 50 2e-06
At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 47 1e-05
At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 45 5e-05
At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 45 5e-05
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 45 5e-05
At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 45 6e-05
At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 43 2e-04
At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4) ... 30 1.9
At5g19250.1 68418.m02292 expressed protein 29 2.5
At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3) ... 29 3.3
At5g55300.1 68418.m06891 DNA topoisomerase I identical to Swiss-... 28 5.7
At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /... 28 5.7
At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative... 28 5.7
At5g61080.1 68418.m07664 expressed protein predicted proteins, A... 28 7.5
At3g07140.2 68416.m00851 GPI transamidase component Gpi16 subuni... 28 7.5
At3g07140.1 68416.m00850 GPI transamidase component Gpi16 subuni... 28 7.5
At3g56410.2 68416.m06274 expressed protein 27 9.9
At3g56410.1 68416.m06273 expressed protein 27 9.9
At1g11420.1 68414.m01312 agenet domain-containing protein contai... 27 9.9
>At3g15850.1 68416.m02005 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 371
Score = 51.2 bits (117), Expect = 7e-07
Identities = 25/66 (37%), Positives = 37/66 (56%)
Frame = +1
Query: 514 HHKYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQ 693
HH++ ++D DPH+ GF+FSH+ W + + + G +V DL P RF + Y
Sbjct: 188 HHQFCDSDRDPHSPLDGFWFSHMNW-MFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTY- 245
Query: 694 ILMPLA 711
IL PLA
Sbjct: 246 ILHPLA 251
Score = 41.1 bits (92), Expect = 8e-04
Identities = 23/65 (35%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = +2
Query: 317 FKAMWRTSIFAIFLYLCSGL-GITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVD 493
F+ WR A LY+ +GL GIT HR +HK++K L L A Q +D
Sbjct: 121 FQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPID 180
Query: 494 WARDH 508
W H
Sbjct: 181 WVSTH 185
>At3g15870.1 68416.m02007 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 292
Score = 50.4 bits (115), Expect = 1e-06
Identities = 24/69 (34%), Positives = 38/69 (55%)
Frame = +1
Query: 514 HHKYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQ 693
HHK+ ET DPH+ T+GF+FSH+ W + ++ G +V+DL P RF ++
Sbjct: 111 HHKHCETQRDPHSPTQGFWFSHMAW-IFDTSSILENCGGEENVDDLVRQPFYRFLQRTVL 169
Query: 694 ILMPLACFI 720
+ M F+
Sbjct: 170 LHMMAYSFL 178
Score = 34.3 bits (75), Expect = 0.087
Identities = 19/70 (27%), Positives = 33/70 (47%)
Frame = +2
Query: 299 GGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQ 478
G L +A W +F +++ +G+ IT HR +H+S+ L L +AFQ
Sbjct: 43 GSILLQLEAFW---VFPWLVFI-NGICITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQ 98
Query: 479 DAVVDWARDH 508
++W +H
Sbjct: 99 GDPIEWVSNH 108
>At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to
delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from
[Arabidopsis thaliana]
Length = 307
Score = 50.0 bits (114), Expect = 2e-06
Identities = 32/104 (30%), Positives = 43/104 (41%)
Frame = +2
Query: 272 FILHVGGVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILL 451
F +H + + F + A+W T +F Y GLGIT HR AH+S+K L LL
Sbjct: 48 FTVHSLALLAPFYFTWSALWVTFLF----YTIGGLGITVSYHRNLAHRSFKVPKWLEYLL 103
Query: 452 TIFNTIAFQDAVVDWARDHACTTNIQRPMRTPTTQPEDSSFPTL 583
+A Q +DW H R P + E F L
Sbjct: 104 AYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHL 147
Score = 44.8 bits (101), Expect = 6e-05
Identities = 19/57 (33%), Positives = 33/57 (57%)
Frame = +1
Query: 514 HHKYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 684
HH++++++ DPH+ GF+FSH+ W+ + K G +V DL+ RF +K
Sbjct: 125 HHQFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC-GRRANVEDLKRQWFYRFLQK 180
>At1g06100.1 68414.m00639 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 47.2 bits (107), Expect = 1e-05
Identities = 21/57 (36%), Positives = 33/57 (57%)
Frame = +1
Query: 514 HHKYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 684
HH+++++D DPH+ GF+FSH+ W+ + K G +V DL+ RF KK
Sbjct: 117 HHQFTDSDRDPHSPIEGFWFSHVLWIFDTDYIREKC-GRRNNVMDLKQQWFYRFLKK 172
Score = 33.1 bits (72), Expect = 0.20
Identities = 19/64 (29%), Positives = 25/64 (39%)
Frame = +2
Query: 317 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 496
F W F I L + + L IT HR H+S+K L +A Q +DW
Sbjct: 51 FNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDW 110
Query: 497 ARDH 508
H
Sbjct: 111 VSIH 114
>At1g06360.1 68414.m00672 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 45.2 bits (102), Expect = 5e-05
Identities = 17/47 (36%), Positives = 31/47 (65%)
Frame = +1
Query: 514 HHKYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLR 654
HH+++++D DPH+ GF+FSH+ W+ ++ + K G +V DL+
Sbjct: 117 HHQFTDSDRDPHSPIEGFWFSHVWWICDTRYIKYKCGGRN-NVMDLK 162
Score = 40.7 bits (91), Expect = 0.001
Identities = 22/64 (34%), Positives = 26/64 (40%)
Frame = +2
Query: 317 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 496
F W F LY + L IT HR AH+S+K L L F A Q +DW
Sbjct: 51 FNYKWEALRFGFVLYALTSLSITFSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDW 110
Query: 497 ARDH 508
H
Sbjct: 111 VSIH 114
>At1g06120.1 68414.m00641 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported
by cDNA:gi_12083275_gb_AF332434.1_AF332434
Length = 299
Score = 45.2 bits (102), Expect = 5e-05
Identities = 19/54 (35%), Positives = 31/54 (57%)
Frame = +1
Query: 514 HHKYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 675
HH+++++D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 117 HHQFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
Score = 33.1 bits (72), Expect = 0.20
Identities = 18/64 (28%), Positives = 24/64 (37%)
Frame = +2
Query: 317 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 496
F W FA + + + L IT HR H+S+K L A Q +DW
Sbjct: 51 FNYTWEAFRFAAMVGISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDW 110
Query: 497 ARDH 508
H
Sbjct: 111 VSTH 114
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 45.2 bits (102), Expect = 5e-05
Identities = 19/54 (35%), Positives = 31/54 (57%)
Frame = +1
Query: 514 HHKYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 675
HH+++++D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 117 HHQFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
Score = 35.9 bits (79), Expect = 0.028
Identities = 19/64 (29%), Positives = 24/64 (37%)
Frame = +2
Query: 317 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 496
F W F + L + + L IT HR HKS+K L A Q +DW
Sbjct: 51 FNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDW 110
Query: 497 ARDH 508
H
Sbjct: 111 VSTH 114
>At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to
delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180
GI:2970034 from [Arabidopsis thaliana]
Length = 305
Score = 44.8 bits (101), Expect = 6e-05
Identities = 21/64 (32%), Positives = 37/64 (57%), Gaps = 2/64 (3%)
Frame = +1
Query: 514 HHKYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK--Y 687
HH+++++D DPH+ GF+FSH+ WL + ++ G +V DL+ +F ++
Sbjct: 123 HHQFTDSDRDPHSPNEGFWFSHLLWLFDTGY-LVEKCGRRTNVEDLKRQWYYKFLQRTVL 181
Query: 688 YQIL 699
Y IL
Sbjct: 182 YHIL 185
Score = 43.6 bits (98), Expect = 1e-04
Identities = 21/64 (32%), Positives = 27/64 (42%)
Frame = +2
Query: 317 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 496
F W A+ +Y GLGIT HR AH+S+K L +A Q +DW
Sbjct: 57 FNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDW 116
Query: 497 ARDH 508
H
Sbjct: 117 VSTH 120
>At1g06350.1 68414.m00671 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 300
Score = 43.2 bits (97), Expect = 2e-04
Identities = 18/57 (31%), Positives = 33/57 (57%)
Frame = +1
Query: 514 HHKYSETDADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 684
HH+++++D DPH+ G FSHI W+ ++ + K G +V DL+ +F ++
Sbjct: 118 HHQFTDSDRDPHSPKEGLLFSHILWIFDTQYIKYKCGGRD-NVLDLKKQWFYKFLRR 173
>At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4)
identical to metal transporter Nramp4 [Arabidopsis
thaliana] gi|6468014|gb|AAF13279; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 512
Score = 29.9 bits (64), Expect = 1.9
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +2
Query: 290 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 397
G GY ++ MW T+I + L + LG+ G H
Sbjct: 79 GAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRH 114
>At5g19250.1 68418.m02292 expressed protein
Length = 196
Score = 29.5 bits (63), Expect = 2.5
Identities = 18/60 (30%), Positives = 24/60 (40%)
Frame = +2
Query: 416 SYKARLPLRILLTIFNTIAFQDAVVDWARDHACTTNIQRPMRTPTTQPEDSSFPTLAGCC 595
SY+A L L L+ N D + D ++ C TN P T P + P L C
Sbjct: 39 SYRASLNLTTLIHNHNAECLADEIADQFKNQPC-TNTTGSASVPGTTPGFPNLPNLLSKC 97
>At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3)
identical to metal transporter Nramp3 [Arabidopsis
thaliana] gi|6468012|gb|AAF13278; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 509
Score = 29.1 bits (62), Expect = 3.3
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +2
Query: 290 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 397
G GY L+ MW T++ + L + LG+ G H
Sbjct: 83 GAVAGYSLLWLLMWATAMGLLVQLLSARLGVATGRH 118
>At5g55300.1 68418.m06891 DNA topoisomerase I identical to
Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis
thaliana]
Length = 916
Score = 28.3 bits (60), Expect = 5.7
Identities = 15/46 (32%), Positives = 20/46 (43%)
Frame = +2
Query: 500 RDHACTTNIQRPMRTPTTQPEDSSFPTLAGCC*GSIPKSKPKAIPS 637
R A T PMR+P T P ++ S+P S KA P+
Sbjct: 45 RSKAVPTTKVSPMRSPVTSPNGTTPSNKTSIVKSSMPSSSSKASPA 90
>At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Lycopersicon esculentum] GI:7381227;
contains PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 468
Score = 28.3 bits (60), Expect = 5.7
Identities = 15/58 (25%), Positives = 24/58 (41%)
Frame = +3
Query: 156 SWVLYEADANNLPEDAPPHVPPSAEKRPWKIVWEM*FCSLFCMSVVFMEGTCSFSKPC 329
S+ ++ D +P D P P S KR W + + +L V+ G + PC
Sbjct: 124 SYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPC 181
>At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative
(NRAMP2) similar to metal transporter Nramp3
[Arabidopsis thaliana] gi|6468012|gb|AAF13278; member of
the natural resistance-associated macrophage protein
(NRAMP) metal transporter family, PMID:11500563
Length = 530
Score = 28.3 bits (60), Expect = 5.7
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +2
Query: 290 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 397
G GY L+ MW T++ + L + +G+ G H
Sbjct: 95 GAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRH 130
>At5g61080.1 68418.m07664 expressed protein predicted proteins,
Arabidopsis thaliana; expression supported by MPSS
Length = 348
Score = 27.9 bits (59), Expect = 7.5
Identities = 20/68 (29%), Positives = 28/68 (41%)
Frame = -2
Query: 412 VCPKSVRASCDTQSRTQVQKYGEYRSPPHGFEKEQVPSINTTDMQNKEQNHISHTIFQGL 233
V K + S T SR+ + GE PP GFEK P + ++Q T G+
Sbjct: 157 VALKLITESQKTPSRSPEKVKGEASVPP-GFEKIWTPPLVKLNIQGTSNPLSDLTRSAGI 215
Query: 232 FSAEGGTW 209
+ G W
Sbjct: 216 VRDQSGKW 223
>At3g07140.2 68416.m00851 GPI transamidase component Gpi16 subunit
family protein similar to phosphatidyl inositol glycan
class T (GI:14456615) [Homo sapiens]; contains Pfam
profile PF04113: Gpi16 subunit, GPI transamidase
component
Length = 643
Score = 27.9 bits (59), Expect = 7.5
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 213 VPPSAEKRPWKIVWEM*FCSLFCMSVVFMEGTCSFSKPCGG 335
VP S WK + LFC S+ F+E + S++ P G
Sbjct: 122 VPQSQVDTSWKNLTHA-LSGLFCASINFLESSTSYAAPTWG 161
>At3g07140.1 68416.m00850 GPI transamidase component Gpi16 subunit
family protein similar to phosphatidyl inositol glycan
class T (GI:14456615) [Homo sapiens]; contains Pfam
profile PF04113: Gpi16 subunit, GPI transamidase
component
Length = 644
Score = 27.9 bits (59), Expect = 7.5
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 213 VPPSAEKRPWKIVWEM*FCSLFCMSVVFMEGTCSFSKPCGG 335
VP S WK + LFC S+ F+E + S++ P G
Sbjct: 122 VPQSQVDTSWKNLTHA-LSGLFCASINFLESSTSYAAPTWG 161
>At3g56410.2 68416.m06274 expressed protein
Length = 1535
Score = 27.5 bits (58), Expect = 9.9
Identities = 15/49 (30%), Positives = 24/49 (48%)
Frame = +1
Query: 145 NRRLRGYCTKPTPITCQKMHRPMYLPQQRRDLGRLCGKCDSVLYSACRW 291
+R + G ++ + C K H+ + P CG CDS+L+ A RW
Sbjct: 54 SRTVPGLSSQSRIVRCPKCHKLLQEPLDATSYK--CGGCDSILH-AKRW 99
>At3g56410.1 68416.m06273 expressed protein
Length = 1488
Score = 27.5 bits (58), Expect = 9.9
Identities = 15/49 (30%), Positives = 24/49 (48%)
Frame = +1
Query: 145 NRRLRGYCTKPTPITCQKMHRPMYLPQQRRDLGRLCGKCDSVLYSACRW 291
+R + G ++ + C K H+ + P CG CDS+L+ A RW
Sbjct: 7 SRTVPGLSSQSRIVRCPKCHKLLQEPLDATSYK--CGGCDSILH-AKRW 52
>At1g11420.1 68414.m01312 agenet domain-containing protein contains
Pfam PF05641: Agenet domain
Length = 604
Score = 27.5 bits (58), Expect = 9.9
Identities = 10/35 (28%), Positives = 17/35 (48%)
Frame = -2
Query: 313 EQVPSINTTDMQNKEQNHISHTIFQGLFSAEGGTW 209
++ P++ N E+NH HTI F+ + W
Sbjct: 381 DRTPNVVKNSASNAEENHAKHTIMVLPFAKKSPVW 415
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,633,596
Number of Sequences: 28952
Number of extensions: 456868
Number of successful extensions: 1265
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 1198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1264
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1643603136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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