Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0381
(739 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar ... 52 4e-07
At1g09580.1 68414.m01075 emp24/gp25L/p24 family protein similar ... 50 1e-06
At1g21900.1 68414.m02741 emp24/gp25L/p24 family protein similar ... 50 2e-06
At1g69460.1 68414.m07981 emp24/gp25L/p24 family protein similar ... 45 5e-05
At1g26690.1 68414.m03251 emp24/gp25L/p24 family protein similar ... 43 2e-04
At3g29070.1 68416.m03636 emp24/gp25L/p24 family protein similar ... 42 6e-04
At2g03290.1 68415.m00284 emp24/gp25L/p24 family protein similar ... 41 7e-04
At1g14010.1 68414.m01654 emp24/gp25L/p24 family protein similar ... 41 7e-04
At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contain... 40 0.002
At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar ... 33 0.26
At3g13677.1 68416.m01726 expressed protein ; expression supporte... 28 5.6
At2g37680.1 68415.m04621 phytochrome A specific signal transduct... 28 5.6
At1g22060.1 68414.m02759 expressed protein 28 7.4
At3g14470.1 68416.m01833 disease resistance protein (NBS-LRR cla... 27 9.8
>At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 212
Score = 52.0 bits (119), Expect = 4e-07
Identities = 26/60 (43%), Positives = 40/60 (66%), Gaps = 1/60 (1%)
Frame = +3
Query: 513 KLKPMEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSMA-CLFGSG 689
K++ +E+E K+LE EAI ++ +R RE EMR +E TN+RV ++SI S+ C+ SG
Sbjct: 137 KIEGVELEFKKLEGAVEAIHENLIYLRNREAEMRIVSEKTNSRVAWYSIMSLGICIVVSG 196
Score = 36.3 bits (80), Expect = 0.021
Identities = 33/145 (22%), Positives = 62/145 (42%), Gaps = 6/145 (4%)
Frame = +1
Query: 322 GHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHLVSLDIKIGIEAKTYEGI 501
G +L ++ T G+F+F T+ + CF + S +++D K GI AK ++ I
Sbjct: 74 GTVLHHRENTTNGQFAFTTQESGTYLACFEADAKSHGNK-DFSINIDWKTGIAAKDWDSI 132
Query: 502 GEVQN*SQWKWN*RD*KTYQKLSFKISHXXXXXXXX*EIPMNPRIIEFYSSVYSRWRA-- 675
+ + ++KL + RI+ ++ W +
Sbjct: 133 ARKEKIEGVELE------FKKLEGAVEAIHENLIYLRNREAEMRIVSEKTNSRVAWYSIM 186
Query: 676 SLGL----ATWQVLYLRRFFKAKKL 738
SLG+ + Q+LYL+++F+ KKL
Sbjct: 187 SLGICIVVSGLQILYLKQYFEKKKL 211
>At1g09580.1 68414.m01075 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 217
Score = 50.4 bits (115), Expect = 1e-06
Identities = 24/60 (40%), Positives = 41/60 (68%), Gaps = 1/60 (1%)
Frame = +3
Query: 513 KLKPMEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSM-ACLFGSG 689
K++ +E+E+++LE EAI ++ +R RE +MR +E TN+RV ++SI S+ C+ SG
Sbjct: 142 KIEGVELEIRKLEGAVEAIHENILYLRNREADMRTMSEKTNSRVAWYSIMSLGVCIAVSG 201
Score = 30.3 bits (65), Expect = 1.4
Identities = 35/144 (24%), Positives = 63/144 (43%), Gaps = 5/144 (3%)
Frame = +1
Query: 322 GHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSE-RRGIPHLVSLDIKIGIEAKTYEG 498
G+ L + VT G+F+F T+ + CF + S + + +++D + GI AK +
Sbjct: 79 GNNLHNMENVTHGQFAFTTQESGNYLACFWADEKSHGNKNVS--INIDWRTGIAAKDWAS 136
Query: 499 IGEVQN*SQWKWN*RD*KTYQKLSFKISHXXXXXXXX*EIPMNPRIIEFYSSVYSRWRAS 678
I + + + R + + + H E M + S V S
Sbjct: 137 IAKKEKIEGVELEIRK----LEGAVEAIHENILYLRNREADMRTMSEKTNSRVAWYSIMS 192
Query: 679 LG----LATWQVLYLRRFFKAKKL 738
LG ++ +QVLYL+++F+ KKL
Sbjct: 193 LGVCIAVSGFQVLYLKQYFEKKKL 216
>At1g21900.1 68414.m02741 emp24/gp25L/p24 family protein similar to
SP|O35587 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Mesocricetus
auratus}; contains Pfam profile PF01105: emp24/gp25L/p24
family
Length = 216
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 39/52 (75%)
Frame = +3
Query: 513 KLKPMEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSM 668
K++ +E++L+RLE L +I ++ ++ RE EMR+ +E+TN+RV +FSI S+
Sbjct: 141 KIEGVELQLRRLEGLVLSIRENLNYIKDREAEMREVSETTNSRVAWFSIMSL 192
Score = 34.3 bits (75), Expect = 0.086
Identities = 18/62 (29%), Positives = 32/62 (51%)
Frame = +1
Query: 322 GHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHLVSLDIKIGIEAKTYEGI 501
G+ L ++ VT G+F+F T+ + CF S P + +D K+GI AK ++ +
Sbjct: 78 GNNLHHQENVTHGQFAFTTQEAGNYLACFWID-SSHHLANPITLGVDWKMGIAAKDWDSV 136
Query: 502 GE 507
+
Sbjct: 137 AK 138
>At1g69460.1 68414.m07981 emp24/gp25L/p24 family protein similar to
SP|Q28735 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Oryctolagus
cuniculus}; contains Pfam profile: PF01105:
emp24/gp25L/p24 family
Length = 214
Score = 45.2 bits (102), Expect = 5e-05
Identities = 21/58 (36%), Positives = 35/58 (60%)
Frame = +3
Query: 510 AKLKPMEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSMACLFG 683
++++ ME E+K L D +I ++ +R REEEM+D N STN ++ + S+ S G
Sbjct: 138 SQVEVMEFEVKSLLDTVNSIHEEMYYLRDREEEMQDLNRSTNTKMAWLSVLSFFVCIG 195
>At1g26690.1 68414.m03251 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 214
Score = 42.7 bits (96), Expect = 2e-04
Identities = 20/61 (32%), Positives = 41/61 (67%), Gaps = 1/61 (1%)
Frame = +3
Query: 510 AKLKPMEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSM-ACLFGS 686
++++ ME ++KRL + +I ++ +R+REEEM++ N +TN+++ + S S+ CL +
Sbjct: 138 SQVEVMEFDVKRLIETVNSIHEEMFYLREREEEMQNLNRATNSKMAWLSFLSLFVCLGVA 197
Query: 687 G 689
G
Sbjct: 198 G 198
>At3g29070.1 68416.m03636 emp24/gp25L/p24 family protein similar to
SP|Q28735 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Oryctolagus
cuniculus}; contains Pfam profile: PF01105
emp24/gp25L/p24 family
Length = 204
Score = 41.5 bits (93), Expect = 6e-04
Identities = 18/47 (38%), Positives = 33/47 (70%)
Frame = +3
Query: 525 MEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFS 665
+EVE+++L D++E I ++ + +RE EM++ N STN+R+ S+ S
Sbjct: 133 LEVEVRKLLDVTETIHEEMFQLIEREREMQELNRSTNSRMAALSLLS 179
Score = 32.7 bits (71), Expect = 0.26
Identities = 19/67 (28%), Positives = 28/67 (41%)
Frame = +1
Query: 307 IKDSKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHLVSLDIKIGIEAK 486
+ KG + V GKF F E + CF++ P R V + K G+EAK
Sbjct: 62 VSSPKGKSHHHAENVESGKFVFTAEETGDYMTCFVA--PGYRPTAKFAVDFEWKSGVEAK 119
Query: 487 TYEGIGE 507
+ I +
Sbjct: 120 DWTTIAK 126
>At2g03290.1 68415.m00284 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 170
Score = 41.1 bits (92), Expect = 7e-04
Identities = 21/56 (37%), Positives = 36/56 (64%), Gaps = 1/56 (1%)
Frame = +3
Query: 525 MEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSM-ACLFGSG 689
ME ++K L D +I ++ +R+REEEM++ N STN+++ + S S+ CL +G
Sbjct: 99 MEYQVKTLMDTVISIHEEMYYLREREEEMQELNRSTNSKMAWLSFGSLVVCLSVAG 154
>At1g14010.1 68414.m01654 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 212
Score = 41.1 bits (92), Expect = 7e-04
Identities = 21/61 (34%), Positives = 38/61 (62%), Gaps = 1/61 (1%)
Frame = +3
Query: 510 AKLKPMEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSMA-CLFGS 686
++++ ME E+K+L + I + +R REEEM + N +TN+++ + S S+A CL +
Sbjct: 136 SQVETMEFEVKKLFETVNGIHDEMFYLRDREEEMHNLNIATNSKMAWLSFVSLAVCLSVA 195
Query: 687 G 689
G
Sbjct: 196 G 196
>At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contains
weak similarity to transmembrane protein (GI:1212965)
[Homo sapiens]
Length = 214
Score = 39.9 bits (89), Expect = 0.002
Identities = 16/48 (33%), Positives = 30/48 (62%)
Frame = +3
Query: 516 LKPMEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSI 659
L P+ V++ L + E++V + ++ R+ R TNEST RV+F+++
Sbjct: 137 LDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVIFYTV 184
>At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar to
SP|O35587 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Mesocricetus
auratus}; contains Pfam profile: PF01105 emp24/gp25L/p24
family; contains non-consensus TG acceptor splice site
at exon 3
Length = 217
Score = 32.7 bits (71), Expect = 0.26
Identities = 38/156 (24%), Positives = 63/156 (40%), Gaps = 5/156 (3%)
Frame = +1
Query: 286 GQRVDYIIKDSKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPH--LVSL 459
G +D + G L + VT G+ +F T F C + +VSL
Sbjct: 64 GPTLDVRVTSPYGKELYKIANVTHGQAAFTTSESGTFLACLAMHHDQSHHSVNSSVIVSL 123
Query: 460 DIKIGIEAKTYEGIGEVQN*SQWKWN*RD*KTY-QKLSFKISHXXXXXXXX*EI--PMNP 630
D K+GI AK ++ + + + + R Y + I + EI N
Sbjct: 124 DWKMGIRAKDWDSVAKKEKIEAMELEIRRSTEYASAIRANILYLRIREAYMREINEKTNT 183
Query: 631 RIIEFYSSVYSRWRASLGLATWQVLYLRRFFKAKKL 738
R+ + + S ++ ++ QVLYL+R+F KKL
Sbjct: 184 RVNQL--GLMS-LGVAIVVSISQVLYLKRYFLKKKL 216
>At3g13677.1 68416.m01726 expressed protein ; expression supported
by MPSS
Length = 171
Score = 28.3 bits (60), Expect = 5.6
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2
Query: 485 RLMKGSGRCKIEANGSGTEETRRPI 559
R+MK K+E NG+ EE RRP+
Sbjct: 100 RMMKTHKSLKLEVNGTFREEVRRPL 124
>At2g37680.1 68415.m04621 phytochrome A specific signal transduction
component (PAT3) / far-red elongated hypocotyl protein 1
(FHY1) identical to phytochrome A specific signal
transduction component PAT3 [Arabidopsis thaliana]
gi|19421998|gb|AAL87850; identical to far-red elongated
hypocotyl protein 1 [Arabidopsis thaliana]
gi|17148773|gb|AAL35819
Length = 313
Score = 28.3 bits (60), Expect = 5.6
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = +3
Query: 540 KRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLF 650
+ LED SE ++++ + ++EEE D ++N F
Sbjct: 258 QNLEDFSEKVIENILYLDEQEEEEEDAKGCSSNAAKF 294
>At1g22060.1 68414.m02759 expressed protein
Length = 1999
Score = 27.9 bits (59), Expect = 7.4
Identities = 15/56 (26%), Positives = 33/56 (58%)
Frame = +3
Query: 498 DRGGAKLKPMEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFS 665
++ G K +P++ ++ LED+ ++ +L +K EEE+ + ++R L+ +FS
Sbjct: 695 EKKGMKERPLKGDIILLEDMKRSLHVQESLYQKVEEELYE----MHSRNLYLEVFS 746
>At3g14470.1 68416.m01833 disease resistance protein (NBS-LRR
class), putative domain signature NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1054
Score = 27.5 bits (58), Expect = 9.8
Identities = 15/51 (29%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
Frame = -3
Query: 260 PALTRWRVINLSNTCVCSAPSST*SHQFHARLSSVTTRDI--VPPCLLWLY 114
P LTR RV++LS+ + P + HAR ++ ++ +P L ++Y
Sbjct: 575 PTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMY 625
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,364,468
Number of Sequences: 28952
Number of extensions: 305156
Number of successful extensions: 838
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 835
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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