Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0379
(802 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g75270.1 68414.m08744 dehydroascorbate reductase, putative si... 32 0.51
At5g62480.1 68418.m07841 glutathione S-transferase, putative 30 1.6
At5g37460.1 68418.m04508 hypothetical protein contains Pfam PF04... 30 1.6
At5g28885.1 68418.m03555 hypothetical protein 30 1.6
At3g18680.2 68416.m02373 aspartate/glutamate/uridylate kinase fa... 30 2.1
At3g18680.1 68416.m02372 aspartate/glutamate/uridylate kinase fa... 30 2.1
At5g58840.1 68418.m07373 subtilase family protein contains simil... 29 2.7
At5g27650.1 68418.m03313 PWWP domain-containing protein hypothet... 29 2.7
At4g19160.3 68417.m02827 expressed protein 28 6.3
At4g19160.2 68417.m02826 expressed protein 28 6.3
At4g19160.1 68417.m02828 expressed protein 28 6.3
At5g59130.1 68418.m07411 subtilase family protein contains simil... 28 8.3
At1g27130.1 68414.m03306 glutathione S-transferase, putative sim... 28 8.3
>At1g75270.1 68414.m08744 dehydroascorbate reductase, putative
similar to GI:6939839 from [Oryza sativa]
Length = 213
Score = 31.9 bits (69), Expect = 0.51
Identities = 14/30 (46%), Positives = 22/30 (73%)
Frame = +2
Query: 524 SKKGQLPFVELNGEEIADSTFIIKDLSEKY 613
S +G++P V+L+G+ +ADS I+ L EKY
Sbjct: 55 SPEGKVPVVKLDGKWVADSDVIVGLLEEKY 84
>At5g62480.1 68418.m07841 glutathione S-transferase, putative
Length = 240
Score = 30.3 bits (65), Expect = 1.6
Identities = 23/86 (26%), Positives = 40/86 (46%)
Frame = +3
Query: 441 SPYCLKVETWLRLAGIKYENVEHMPNIVPKKANCRSWN*MAKK*PIALSSSKIFPRNTTK 620
SPY ++E LRL I Y+ V+ ++ K +N + KK P+ + + K +
Sbjct: 17 SPYSKRIELALRLKSIPYQFVQE--DLQNKSQTLLRYNPVHKKIPVLVHNGKPISESLFI 74
Query: 621 ILMLV*RRSSGLYLTP*SP*SRTTCR 698
I + S+G ++ P P R+ R
Sbjct: 75 IEYIDETWSNGPHILPEDPYRRSKVR 100
>At5g37460.1 68418.m04508 hypothetical protein contains Pfam PF04510
: Family of unknown function (DUF577)); common family
comprised of At5g37410, At5g37400, At5g37920,
At5g37460, At5g37650, At5g37470, At5g37420, At5g37430
Length = 622
Score = 30.3 bits (65), Expect = 1.6
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -2
Query: 321 FHRLSHQVMEPLWIGHYRPARPLHVFLFLRSVFRDVIRNF 202
+H LS + LW+ P + HVF+ L SV++ I F
Sbjct: 141 WHELSDCI---LWLAENEPVKAFHVFIDLPSVYKSFINKF 177
>At5g28885.1 68418.m03555 hypothetical protein
Length = 184
Score = 30.3 bits (65), Expect = 1.6
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +2
Query: 545 FVELNGEEIADSTFIIKDLSEKYNKDLDAGLTSEQRVISHAMISM 679
F++++GE I S I+KD S+ +K LD L + I + M
Sbjct: 94 FIDVSGEIIGMSEIIVKDCSDNMSKLLDIQLRDLSQTIPECTLRM 138
>At3g18680.2 68416.m02373 aspartate/glutamate/uridylate kinase
family protein similar to UMP-kinase GB:CAB38122
gi:4468612 from [Lactococcus lactis] ; contains Pfam
profile PF00696: Amino acid kinase family
Length = 366
Score = 29.9 bits (64), Expect = 2.1
Identities = 17/42 (40%), Positives = 24/42 (57%)
Frame = -2
Query: 657 ITRCSDVRPASRSLLYFSERSLMMKVLSAISSPFSSTNGSWP 532
I+R S V R+ +FS ++ +VL + SS SS NGS P
Sbjct: 19 ISRTSFVPLTLRNRTFFSNQNYSRRVLISCSSSLSSDNGSSP 60
>At3g18680.1 68416.m02372 aspartate/glutamate/uridylate kinase
family protein similar to UMP-kinase GB:CAB38122
gi:4468612 from [Lactococcus lactis] ; contains Pfam
profile PF00696: Amino acid kinase family
Length = 339
Score = 29.9 bits (64), Expect = 2.1
Identities = 17/42 (40%), Positives = 24/42 (57%)
Frame = -2
Query: 657 ITRCSDVRPASRSLLYFSERSLMMKVLSAISSPFSSTNGSWP 532
I+R S V R+ +FS ++ +VL + SS SS NGS P
Sbjct: 19 ISRTSFVPLTLRNRTFFSNQNYSRRVLISCSSSLSSDNGSSP 60
>At5g58840.1 68418.m07373 subtilase family protein contains
similarity to prepro-cucumisin GI:807698 from [Cucumis
melo]; non-consensus acceptor site TT at exon 6
Length = 713
Score = 29.5 bits (63), Expect = 2.7
Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = -1
Query: 754 LVSLNNTVRVLGAPPENHPRQVVLDHGDH-GVRYNPLLRRQTSIK 623
+V+ N TV +G P + ++VL+HG + V +P + S+K
Sbjct: 623 IVTFNRTVTNVGTPNSTYKSKIVLNHGSNLKVEVSPSVLSMKSVK 667
>At5g27650.1 68418.m03313 PWWP domain-containing protein
hypothetical protein F22F7.12 - Arabidopsis thaliana,
EMBL:AC009606
Length = 1072
Score = 29.5 bits (63), Expect = 2.7
Identities = 25/71 (35%), Positives = 38/71 (53%), Gaps = 2/71 (2%)
Frame = +1
Query: 184 NSNMTVEVTNNIPENAPEKE-KDMKRTRRPIMANPKRLHYLMRKAMKNLKR-KNPNQLSR 357
+S+ TVE N + P K K +KRT P A KRL + + + K+ K NQL +
Sbjct: 685 SSDATVE---NARDTKPSKPVKTVKRTEDPSKAGKKRLSSDRQDEIPSAKKLKKTNQL-K 740
Query: 358 NQQSIKQILRK 390
+ S K+I+R+
Sbjct: 741 SMASEKKIIRE 751
>At4g19160.3 68417.m02827 expressed protein
Length = 454
Score = 28.3 bits (60), Expect = 6.3
Identities = 16/58 (27%), Positives = 31/58 (53%)
Frame = +2
Query: 518 YRSKKGQLPFVELNGEEIADSTFIIKDLSEKYNKDLDAGLTSEQRVISHAMISMIENH 691
+++ GQ F +NG + D + DL+ K +DLD + + + +I A+ ++I H
Sbjct: 279 FKATSGQSLFSIVNGRCVDDPGSMASDLTAKSLQDLD--MATNRDIIGIALANLIRLH 334
>At4g19160.2 68417.m02826 expressed protein
Length = 453
Score = 28.3 bits (60), Expect = 6.3
Identities = 16/58 (27%), Positives = 31/58 (53%)
Frame = +2
Query: 518 YRSKKGQLPFVELNGEEIADSTFIIKDLSEKYNKDLDAGLTSEQRVISHAMISMIENH 691
+++ GQ F +NG + D + DL+ K +DLD + + + +I A+ ++I H
Sbjct: 279 FKATSGQSLFSIVNGRCVDDPGSMASDLTAKSLQDLD--MATNRDIIGIALANLIRLH 334
>At4g19160.1 68417.m02828 expressed protein
Length = 312
Score = 28.3 bits (60), Expect = 6.3
Identities = 16/58 (27%), Positives = 31/58 (53%)
Frame = +2
Query: 518 YRSKKGQLPFVELNGEEIADSTFIIKDLSEKYNKDLDAGLTSEQRVISHAMISMIENH 691
+++ GQ F +NG + D + DL+ K +DLD + + + +I A+ ++I H
Sbjct: 138 FKATSGQSLFSIVNGRCVDDPGSMASDLTAKSLQDLD--MATNRDIIGIALANLIRLH 193
>At5g59130.1 68418.m07411 subtilase family protein contains
similarity to prepro-cucumisin GI:807698 from [Cucumis
melo]
Length = 732
Score = 27.9 bits (59), Expect = 8.3
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = -1
Query: 754 LVSLNNTVRVLGAPPENHPRQVVLDHGDH-GVRYNP 650
+V+ N TV +G P + +VVL+HG V+ +P
Sbjct: 638 IVTFNRTVTNVGTPNSTYKSKVVLNHGSKLNVKVSP 673
>At1g27130.1 68414.m03306 glutathione S-transferase, putative
similar to glutathione S-transferase GB: AAF22517
GI:6652870 from [Papaver somniferum]
Length = 227
Score = 27.9 bits (59), Expect = 8.3
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Frame = +3
Query: 438 TSPYCLKVETWLRLAGIKYENVEHMPNIVPKKAN-CRSWN*MAKK*PIAL 584
+SPY L+ L L +KYE ++ P+++ +K+ N + KK P+ L
Sbjct: 14 SSPYSLRARVALHLKSVKYEYLDE-PDVLKEKSELLLKSNPIHKKVPVLL 62
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,914,737
Number of Sequences: 28952
Number of extensions: 342987
Number of successful extensions: 978
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 950
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 978
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1814318400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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