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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0377
         (747 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron...   101   5e-22
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri...    32   0.35 
At2g09900.1 68415.m01028 hypothetical protein                          32   0.46 
At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containi...    31   0.81 
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri...    31   0.81 
At4g21865.1 68417.m03162 expressed protein                             29   4.3  
At5g19740.1 68418.m02347 peptidase M28 family protein ileal pept...    28   5.7  
At2g36720.1 68415.m04505 PHD finger transcription factor, putative     28   7.6  
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain...    27   10.0 

>At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong
           similarity to L-lactate dehydrogenase from Lycopersicon
           esculentum (GI:1620970, GI:1620972), Hordeum vulgare
           (SP|P22988, SP|P22989); contains InterPro entry
           IPR001236: Lactate/malate dehydrogenase
          Length = 353

 Score =  101 bits (242), Expect = 5e-22
 Identities = 45/75 (60%), Positives = 57/75 (76%)
 Frame = +1

Query: 514 DKLKGEMMDLQHGSAFMRNAKIQSSTDYSITAGSKICVVTAGVRQREGESRLDLVQRNTD 693
           DKL+GEM+DLQH +AF+   KI +S DY +TAGS +C+VTAG RQ  GESRL+L+QRN  
Sbjct: 76  DKLRGEMLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQNPGESRLNLLQRNVA 135

Query: 694 VLKQIIPQLIKYSPD 738
           + + IIP L K SPD
Sbjct: 136 LFRHIIPPLAKASPD 150



 Score = 42.7 bits (96), Expect = 2e-04
 Identities = 23/48 (47%), Positives = 32/48 (66%), Gaps = 4/48 (8%)
 Frame = +2

Query: 374 FQPVHEKVDETWS----KVTIVGVGQVGMAAAFSMLTQNVTNNIALVD 505
           F+P+H       S    KV++VGVG VGMA A ++LTQ++ + IALVD
Sbjct: 25  FKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTILTQDLADEIALVD 72


>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
           identical to mitochondrial NAD-dependent malate
           dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
           thaliana]; contains InterPro entry IPR001236:
           Lactate/malate dehydrogenase
          Length = 341

 Score = 32.3 bits (70), Expect = 0.35
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = +1

Query: 610 GSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSP 735
           G+ + ++ AGV ++ G +R DL   N  ++K +   + KY P
Sbjct: 97  GADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTAIAKYCP 138


>At2g09900.1 68415.m01028 hypothetical protein
          Length = 175

 Score = 31.9 bits (69), Expect = 0.46
 Identities = 18/43 (41%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
 Frame = -1

Query: 699 KNIGVSLHEIETRFTFALSNTSSNHADLRASCY-RIIRTRLDL 574
           ++I  SLH   TR +  +S T S+H+ +R+S +  IIR  LDL
Sbjct: 28  EDITTSLHHSTTRSSALISITRSHHSTMRSSVFTSIIRQPLDL 70


>At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 822

 Score = 31.1 bits (67), Expect = 0.81
 Identities = 17/54 (31%), Positives = 31/54 (57%), Gaps = 1/54 (1%)
 Frame = +2

Query: 350 KMESLKKLFQPVHEKVDETWSKVTIVGVGQVGMAA-AFSMLTQNVTNNIALVDM 508
           KM++  KLF  +  K + +W+ V IVG   +G    AFSM  + + N +++ ++
Sbjct: 398 KMDTAVKLFAELSSKNEVSWNTV-IVGYENLGEGGKAFSMFREALRNQVSVTEV 450


>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
           putative similar to mitochondrial NAD-dependent malate
           dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
           thaliana]; contains InterPro entry IPR001236:
           Lactate/malate dehydrogenase
          Length = 341

 Score = 31.1 bits (67), Expect = 0.81
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = +1

Query: 610 GSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSP 735
           G+ + ++ AGV ++ G +R DL   N  ++K +   + KY P
Sbjct: 97  GADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLSIAIAKYCP 138


>At4g21865.1 68417.m03162 expressed protein 
          Length = 154

 Score = 28.7 bits (61), Expect = 4.3
 Identities = 15/42 (35%), Positives = 22/42 (52%)
 Frame = -2

Query: 197 PDRSVLIGNRKQNEQNKNKSKMNS*HTKLNMKDNTSQINNVH 72
           PD   +IGN    E+ K K  + S   K ++K   SQ+ NV+
Sbjct: 94  PDNDAMIGNMNMIEERKAKKGLKS---KDHIKSRASQLKNVN 132


>At5g19740.1 68418.m02347 peptidase M28 family protein ileal
           peptidase I100 - Rattus norvegicus, EMBL:AF009921;
           contains Pfam profiles PF04389: Peptidase family M28,
           PF02225: PA domain
          Length = 681

 Score = 28.3 bits (60), Expect = 5.7
 Identities = 10/22 (45%), Positives = 14/22 (63%)
 Frame = -2

Query: 425 QWSPCSKFRPPSRARAGTASSG 360
           +W P SK+ PPS  + GT  +G
Sbjct: 209 EWFPASKWMPPSGVQVGTVYNG 230


>At2g36720.1 68415.m04505 PHD finger transcription factor, putative
          Length = 1007

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 12/35 (34%), Positives = 21/35 (60%)
 Frame = -1

Query: 708 YLFKNIGVSLHEIETRFTFALSNTSSNHADLRASC 604
           Y++ + GVSLHE  T F+     +++++ DL   C
Sbjct: 587 YIYTSNGVSLHEWATTFSHGRKYSANDNNDLCVIC 621


>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
           protein similar to PF02225: PA domain; similar to
           N-acetylated-alpha-linked acidic dipeptidase II
           (NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
           Glutamate carboxypeptidase II (Membrane glutamate
           carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
           acidic dipeptidase I) (NAALADase I)
           (Pteroylpoly-gamma-glutamate
           carboxypeptidase)(Folylpoly-gamma-glutamate
           carboxypeptidase) (FGCP) (Folate hydrolase 1)
           (Prostate-specific membrane antigen homolog) (SP|O77564)
           {Sus scrofa}
          Length = 280

 Score = 27.5 bits (58), Expect = 10.0
 Identities = 10/22 (45%), Positives = 14/22 (63%)
 Frame = -2

Query: 425 QWSPCSKFRPPSRARAGTASSG 360
           +W P SK+ PPS  + GT  +G
Sbjct: 74  EWFPASKWMPPSGFQVGTVYNG 95


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,307,017
Number of Sequences: 28952
Number of extensions: 272453
Number of successful extensions: 793
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 780
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 792
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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