BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0377 (747 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron... 101 5e-22 At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri... 32 0.35 At2g09900.1 68415.m01028 hypothetical protein 32 0.46 At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containi... 31 0.81 At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri... 31 0.81 At4g21865.1 68417.m03162 expressed protein 29 4.3 At5g19740.1 68418.m02347 peptidase M28 family protein ileal pept... 28 5.7 At2g36720.1 68415.m04505 PHD finger transcription factor, putative 28 7.6 At4g07670.1 68417.m01203 protease-associated (PA) domain-contain... 27 10.0 >At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong similarity to L-lactate dehydrogenase from Lycopersicon esculentum (GI:1620970, GI:1620972), Hordeum vulgare (SP|P22988, SP|P22989); contains InterPro entry IPR001236: Lactate/malate dehydrogenase Length = 353 Score = 101 bits (242), Expect = 5e-22 Identities = 45/75 (60%), Positives = 57/75 (76%) Frame = +1 Query: 514 DKLKGEMMDLQHGSAFMRNAKIQSSTDYSITAGSKICVVTAGVRQREGESRLDLVQRNTD 693 DKL+GEM+DLQH +AF+ KI +S DY +TAGS +C+VTAG RQ GESRL+L+QRN Sbjct: 76 DKLRGEMLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQNPGESRLNLLQRNVA 135 Query: 694 VLKQIIPQLIKYSPD 738 + + IIP L K SPD Sbjct: 136 LFRHIIPPLAKASPD 150 Score = 42.7 bits (96), Expect = 2e-04 Identities = 23/48 (47%), Positives = 32/48 (66%), Gaps = 4/48 (8%) Frame = +2 Query: 374 FQPVHEKVDETWS----KVTIVGVGQVGMAAAFSMLTQNVTNNIALVD 505 F+P+H S KV++VGVG VGMA A ++LTQ++ + IALVD Sbjct: 25 FKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTILTQDLADEIALVD 72 >At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial identical to mitochondrial NAD-dependent malate dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis thaliana]; contains InterPro entry IPR001236: Lactate/malate dehydrogenase Length = 341 Score = 32.3 bits (70), Expect = 0.35 Identities = 13/42 (30%), Positives = 24/42 (57%) Frame = +1 Query: 610 GSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSP 735 G+ + ++ AGV ++ G +R DL N ++K + + KY P Sbjct: 97 GADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTAIAKYCP 138 >At2g09900.1 68415.m01028 hypothetical protein Length = 175 Score = 31.9 bits (69), Expect = 0.46 Identities = 18/43 (41%), Positives = 27/43 (62%), Gaps = 1/43 (2%) Frame = -1 Query: 699 KNIGVSLHEIETRFTFALSNTSSNHADLRASCY-RIIRTRLDL 574 ++I SLH TR + +S T S+H+ +R+S + IIR LDL Sbjct: 28 EDITTSLHHSTTRSSALISITRSHHSTMRSSVFTSIIRQPLDL 70 >At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 822 Score = 31.1 bits (67), Expect = 0.81 Identities = 17/54 (31%), Positives = 31/54 (57%), Gaps = 1/54 (1%) Frame = +2 Query: 350 KMESLKKLFQPVHEKVDETWSKVTIVGVGQVGMAA-AFSMLTQNVTNNIALVDM 508 KM++ KLF + K + +W+ V IVG +G AFSM + + N +++ ++ Sbjct: 398 KMDTAVKLFAELSSKNEVSWNTV-IVGYENLGEGGKAFSMFREALRNQVSVTEV 450 >At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial, putative similar to mitochondrial NAD-dependent malate dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis thaliana]; contains InterPro entry IPR001236: Lactate/malate dehydrogenase Length = 341 Score = 31.1 bits (67), Expect = 0.81 Identities = 13/42 (30%), Positives = 24/42 (57%) Frame = +1 Query: 610 GSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSP 735 G+ + ++ AGV ++ G +R DL N ++K + + KY P Sbjct: 97 GADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLSIAIAKYCP 138 >At4g21865.1 68417.m03162 expressed protein Length = 154 Score = 28.7 bits (61), Expect = 4.3 Identities = 15/42 (35%), Positives = 22/42 (52%) Frame = -2 Query: 197 PDRSVLIGNRKQNEQNKNKSKMNS*HTKLNMKDNTSQINNVH 72 PD +IGN E+ K K + S K ++K SQ+ NV+ Sbjct: 94 PDNDAMIGNMNMIEERKAKKGLKS---KDHIKSRASQLKNVN 132 >At5g19740.1 68418.m02347 peptidase M28 family protein ileal peptidase I100 - Rattus norvegicus, EMBL:AF009921; contains Pfam profiles PF04389: Peptidase family M28, PF02225: PA domain Length = 681 Score = 28.3 bits (60), Expect = 5.7 Identities = 10/22 (45%), Positives = 14/22 (63%) Frame = -2 Query: 425 QWSPCSKFRPPSRARAGTASSG 360 +W P SK+ PPS + GT +G Sbjct: 209 EWFPASKWMPPSGVQVGTVYNG 230 >At2g36720.1 68415.m04505 PHD finger transcription factor, putative Length = 1007 Score = 27.9 bits (59), Expect = 7.6 Identities = 12/35 (34%), Positives = 21/35 (60%) Frame = -1 Query: 708 YLFKNIGVSLHEIETRFTFALSNTSSNHADLRASC 604 Y++ + GVSLHE T F+ +++++ DL C Sbjct: 587 YIYTSNGVSLHEWATTFSHGRKYSANDNNDLCVIC 621 >At4g07670.1 68417.m01203 protease-associated (PA) domain-containing protein similar to PF02225: PA domain; similar to N-acetylated-alpha-linked acidic dipeptidase II (NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to Glutamate carboxypeptidase II (Membrane glutamate carboxypeptidase) (mGCP) (N-acetylated-alpha-linked acidic dipeptidase I) (NAALADase I) (Pteroylpoly-gamma-glutamate carboxypeptidase)(Folylpoly-gamma-glutamate carboxypeptidase) (FGCP) (Folate hydrolase 1) (Prostate-specific membrane antigen homolog) (SP|O77564) {Sus scrofa} Length = 280 Score = 27.5 bits (58), Expect = 10.0 Identities = 10/22 (45%), Positives = 14/22 (63%) Frame = -2 Query: 425 QWSPCSKFRPPSRARAGTASSG 360 +W P SK+ PPS + GT +G Sbjct: 74 EWFPASKWMPPSGFQVGTVYNG 95 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,307,017 Number of Sequences: 28952 Number of extensions: 272453 Number of successful extensions: 793 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 780 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 792 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1653386488 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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