Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0370
(711 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI00015B492E Cluster: PREDICTED: hypothetical protein;... 88 2e-16
UniRef50_Q4TBV5 Cluster: Chromosome undetermined SCAF7089, whole... 83 7e-15
UniRef50_UPI00015547F5 Cluster: PREDICTED: hypothetical protein;... 82 1e-14
UniRef50_Q6PGK3 Cluster: RIKEN cDNA 2700097O09 gene; n=14; Eutel... 82 2e-14
UniRef50_UPI000151DF04 Cluster: hypothetical protein LOC553566; ... 71 3e-11
UniRef50_Q9GZD0 Cluster: Putative uncharacterized protein; n=2; ... 71 3e-11
UniRef50_Q55C78 Cluster: Putative uncharacterized protein; n=1; ... 66 1e-09
UniRef50_UPI0000DB7609 Cluster: PREDICTED: similar to C04F5.8; n... 52 1e-05
UniRef50_Q4QDE6 Cluster: Putative uncharacterized protein; n=3; ... 52 2e-05
UniRef50_Q4CW12 Cluster: Putative uncharacterized protein; n=2; ... 41 0.035
UniRef50_Q387W6 Cluster: Putative uncharacterized protein; n=1; ... 36 0.11
UniRef50_UPI000051A210 Cluster: PREDICTED: similar to CG32796-PB... 35 1.7
UniRef50_UPI0000EBC6A7 Cluster: PREDICTED: hypothetical protein;... 33 9.2
UniRef50_Q8WXH0 Cluster: Nesprin-2; n=34; Eutheria|Rep: Nesprin-... 33 9.2
>UniRef50_UPI00015B492E Cluster: PREDICTED: hypothetical protein;
n=1; Nasonia vitripennis|Rep: PREDICTED: hypothetical
protein - Nasonia vitripennis
Length = 399
Score = 88.2 bits (209), Expect = 2e-16
Identities = 40/83 (48%), Positives = 59/83 (71%)
Frame = +2
Query: 257 DEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMMFIFKVVLPKDLE 436
D+FLY +++ +++ + K+Q +YC DC S+N + LI++SHSMSK + IF LP L
Sbjct: 116 DDFLYTEEDIDELVEDGKMQRNYCKDCGSKNTEPLIFISHSMSKEAIYHIFNNRLPV-LV 174
Query: 437 GKTFIDIGSRLGSVLYGAYYLSN 505
K +DIGSRLG+VLYGAY L++
Sbjct: 175 DKVVLDIGSRLGAVLYGAYLLTD 197
Score = 61.3 bits (142), Expect = 2e-08
Identities = 25/67 (37%), Positives = 47/67 (70%), Gaps = 1/67 (1%)
Frame = +1
Query: 514 IMGIEMNKECCDVQEEIIDQFSMNRNRIKVIHSDVLDKKDIITTADIVIFNAVISSLIM- 690
I+G+EMN+E C++Q+EI+ ++ M+ +RI+++H + D +D++ AD++I N +
Sbjct: 201 IIGVEMNEELCNLQKEIVSKYKMD-DRIEILHKKIEDAEDVLKIADVIICNNAFEFYVSE 259
Query: 691 KKHKEIW 711
+KH EIW
Sbjct: 260 EKHIEIW 266
Score = 45.6 bits (103), Expect = 0.001
Identities = 18/45 (40%), Positives = 26/45 (57%)
Frame = +3
Query: 132 LRNIGDAIKKKAPFEAVMPSEKISPPTVGDLADCTDSNTIHLMSF 266
L I + IKK+ P ++PSE + PT G+ ADC T+H+ F
Sbjct: 74 LNTISEDIKKRVPLNGILPSENVQAPTYGENADCLADLTVHVDDF 118
>UniRef50_Q4TBV5 Cluster: Chromosome undetermined SCAF7089, whole
genome shotgun sequence; n=2; Tetraodontidae|Rep:
Chromosome undetermined SCAF7089, whole genome shotgun
sequence - Tetraodon nigroviridis (Green puffer)
Length = 300
Score = 83.0 bits (196), Expect = 7e-15
Identities = 42/116 (36%), Positives = 68/116 (58%), Gaps = 2/116 (1%)
Frame = +2
Query: 227 RLHRFQHYTF--DEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMM 400
++ R H T D FLYD+D+ + + + + +C++C S L ++SHS S S +
Sbjct: 73 KMQRLGHPTHHVDSFLYDEDQVDSLCEAGTLSRSFCLNCGSCRTAPLDFISHSFSISELQ 132
Query: 401 FIFKVVLPKDLEGKTFIDIGSRLGSVLYGAYYLSNVLLLWELK*TKNVVMFKRKLL 568
FIF+ VLP DL G +D+GSRLG+VLYG Y S+ L L+ ++ V ++ ++
Sbjct: 133 FIFQKVLP-DLNGCVLVDVGSRLGAVLYGGYVFSSASQLIGLEISEEFVKLQKDIV 187
Score = 41.5 bits (93), Expect = 0.020
Identities = 17/60 (28%), Positives = 42/60 (70%)
Frame = +1
Query: 493 LFI*CTTIMGIEMNKECCDVQEEIIDQFSMNRNRIKVIHSDVLDKKDIITTADIVIFNAV 672
+F + ++G+E+++E +Q++I++++ + +RIKV+H+DV ++ ++ D++I N V
Sbjct: 163 VFSSASQLIGLEISEEFVKLQKDIVEKYQLT-DRIKVLHTDVCLQQALLHNTDVLIMNNV 221
>UniRef50_UPI00015547F5 Cluster: PREDICTED: hypothetical protein;
n=1; Ornithorhynchus anatinus|Rep: PREDICTED:
hypothetical protein - Ornithorhynchus anatinus
Length = 245
Score = 82.2 bits (194), Expect = 1e-14
Identities = 38/87 (43%), Positives = 56/87 (64%)
Frame = +2
Query: 257 DEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMMFIFKVVLPKDLE 436
D FLYD D + + +++K+ +YC+ C S L ++SHS S + FI++ VLP DL
Sbjct: 122 DAFLYDDDFIDSLCEERKMSRNYCMVCGSHQTAPLGFISHSFSLMELKFIYQHVLP-DLT 180
Query: 437 GKTFIDIGSRLGSVLYGAYYLSNVLLL 517
GK +D+GSRLG+VL+G Y S+ L L
Sbjct: 181 GKILVDVGSRLGAVLFGGYLYSSALQL 207
>UniRef50_Q6PGK3 Cluster: RIKEN cDNA 2700097O09 gene; n=14;
Euteleostomi|Rep: RIKEN cDNA 2700097O09 gene - Mus
musculus (Mouse)
Length = 301
Score = 81.8 bits (193), Expect = 2e-14
Identities = 38/87 (43%), Positives = 55/87 (63%)
Frame = +2
Query: 257 DEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMMFIFKVVLPKDLE 436
D FLYD+D + + ++ K+ +YC C S L ++SHS S + + FI+ VLP DL
Sbjct: 85 DAFLYDEDFIDTLCEEGKMSRNYCTVCGSHRTAPLGFISHSFSLTELKFIYHHVLP-DLS 143
Query: 437 GKTFIDIGSRLGSVLYGAYYLSNVLLL 517
GK +D+GSRLG+VLYG Y S+ + L
Sbjct: 144 GKVLVDVGSRLGTVLYGGYLYSSAVQL 170
Score = 49.2 bits (112), Expect = 1e-04
Identities = 19/53 (35%), Positives = 36/53 (67%)
Frame = +1
Query: 514 IMGIEMNKECCDVQEEIIDQFSMNRNRIKVIHSDVLDKKDIITTADIVIFNAV 672
+ G+E+N + C +QE II ++ +RIKV+H+D+ + ++ TAD+++ N V
Sbjct: 170 LCGVELNGQFCQLQEMIIKKYQFG-DRIKVLHADICTQSSLLQTADVIVMNNV 221
>UniRef50_UPI000151DF04 Cluster: hypothetical protein LOC553566;
n=1; Danio rerio|Rep: hypothetical protein LOC553566 -
Danio rerio
Length = 205
Score = 70.9 bits (166), Expect = 3e-11
Identities = 32/77 (41%), Positives = 49/77 (63%)
Frame = +2
Query: 257 DEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMMFIFKVVLPKDLE 436
D FLYD + + + ++ K+ YC++C S L ++SHS S + F+F VLP +L
Sbjct: 85 DAFLYDDETVDSLCEEGKMSRTYCLNCGSHRTAPLEFISHSFSIPELQFLFHNVLP-ELT 143
Query: 437 GKTFIDIGSRLGSVLYG 487
GK +D+GSRLG+VL+G
Sbjct: 144 GKLVLDVGSRLGAVLFG 160
>UniRef50_Q9GZD0 Cluster: Putative uncharacterized protein; n=2;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 279
Score = 70.9 bits (166), Expect = 3e-11
Identities = 30/83 (36%), Positives = 55/83 (66%)
Frame = +2
Query: 257 DEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMMFIFKVVLPKDLE 436
D FLY+ D+ E++ ++ ++ YC C+SR+ L ++SHS+S+ + F+F +++P +
Sbjct: 51 DSFLYEDDDVEELVERDELSRTYCPVCSSRDTVPLNFISHSLSRLQVKFLFDLLMPPTCQ 110
Query: 437 GKTFIDIGSRLGSVLYGAYYLSN 505
+DIGSRLG+++YGA +N
Sbjct: 111 --HVLDIGSRLGAIVYGASIFTN 131
>UniRef50_Q55C78 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 362
Score = 65.7 bits (153), Expect = 1e-09
Identities = 30/86 (34%), Positives = 58/86 (67%), Gaps = 3/86 (3%)
Frame = +2
Query: 257 DEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMMFIFKV-VLPK-- 427
D FLY +++ +D+ + +++ +YC+DC S++I+ + ++SHS + + + ++F VL
Sbjct: 141 DGFLYTEEDIDDLIESGELKNNYCLDCASKSIQPINFISHSTTPNQLKYMFSSDVLGGNC 200
Query: 428 DLEGKTFIDIGSRLGSVLYGAYYLSN 505
+L+ T +DIGSRLG++LY Y S+
Sbjct: 201 NLKQSTILDIGSRLGALLYTGYLFSD 226
Score = 49.2 bits (112), Expect = 1e-04
Identities = 23/75 (30%), Positives = 45/75 (60%), Gaps = 2/75 (2%)
Frame = +1
Query: 493 LFI*CTTIMGIEMNKECCDVQEEIIDQFSMNRNRIKVIHSDVLDKKDIITTADIVIFNAV 672
LF ++G E+N+ D+Q+ +I+++ M +RI+++H +++D + +I +DI+ N V
Sbjct: 223 LFSDTKKLIGCEINQYFSDLQKSMINRYKMT-DRIEIVHGNIIDNQQLIQQSDIIYMNNV 281
Query: 673 ISSLIM--KKHKEIW 711
+ KKH E W
Sbjct: 282 LEFFQQDKKKHIEFW 296
>UniRef50_UPI0000DB7609 Cluster: PREDICTED: similar to C04F5.8; n=1;
Apis mellifera|Rep: PREDICTED: similar to C04F5.8 - Apis
mellifera
Length = 326
Score = 52.4 bits (120), Expect = 1e-05
Identities = 25/74 (33%), Positives = 47/74 (63%), Gaps = 1/74 (1%)
Frame = +1
Query: 493 LFI*CTTIMGIEMNKECCDVQEEIIDQFSMNRNRIKVIHSDVLDKKDIITTADIVIFNAV 672
+F I+G+EMNKE C +Q +I+++++MN +RI++++ + DII +DI+I N
Sbjct: 206 IFTSAKKIIGVEMNKELCSLQCDIVNKYNMN-DRIEIVNKRIEMCPDIIQKSDIIIMNNP 264
Query: 673 ISSLIMK-KHKEIW 711
+ + +H +IW
Sbjct: 265 FEFYVSELEHTKIW 278
Score = 47.2 bits (107), Expect = 4e-04
Identities = 27/74 (36%), Positives = 43/74 (58%), Gaps = 1/74 (1%)
Frame = +3
Query: 48 INIEQWINSRAYKK-DLEEKRSLMKSEDILRNIGDAIKKKAPFEAVMPSEKISPPTVGDL 224
I I+ N +K+ L+EK K +I + I D IK + PF+ + PSE I PP +G+
Sbjct: 86 IKIQSVSNDNCHKECSLQEK----KLNEIKKIIID-IKNRIPFDGIFPSEHIVPPIIGEN 140
Query: 225 ADCTDSNTIHLMSF 266
+DC + +T H+ +F
Sbjct: 141 SDCDNKSTKHIDAF 154
Score = 43.6 bits (98), Expect = 0.005
Identities = 28/83 (33%), Positives = 43/83 (51%)
Frame = +2
Query: 257 DEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMMFIFKVVLPKDLE 436
D FLYD +E +++ +K ++ YC DC S N+K+ +K
Sbjct: 152 DAFLYDDEEVDELIEKGELNNLYCSDCGSTNVKQ----------------YK-------- 187
Query: 437 GKTFIDIGSRLGSVLYGAYYLSN 505
K +DIGSRLG+VLYGA+ ++
Sbjct: 188 -KIILDIGSRLGAVLYGAHIFTS 209
>UniRef50_Q4QDE6 Cluster: Putative uncharacterized protein; n=3;
Leishmania|Rep: Putative uncharacterized protein -
Leishmania major
Length = 522
Score = 51.6 bits (118), Expect = 2e-05
Identities = 30/100 (30%), Positives = 53/100 (53%), Gaps = 11/100 (11%)
Frame = +2
Query: 248 YTFDEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMMFIFKVVLPK 427
Y D FLY + + E + + KK+ YC C S ++ + +++HS S+ ++++ +LP
Sbjct: 174 YAVDAFLYAEGDIEKLVEAKKLAREYCCRCGSTDLGLVEFITHSFSQDQLVYLSCFLLPH 233
Query: 428 DLE----------GKTFIDIGSRLGSVLYG-AYYLSNVLL 514
L+ + +D+GSRLG VL+ A+ L LL
Sbjct: 234 LLDVVVASHEASRPLSIVDVGSRLGIVLWACAFALQRGLL 273
>UniRef50_Q4CW12 Cluster: Putative uncharacterized protein; n=2;
Trypanosoma cruzi|Rep: Putative uncharacterized protein
- Trypanosoma cruzi
Length = 449
Score = 40.7 bits (91), Expect = 0.035
Identities = 17/59 (28%), Positives = 34/59 (57%)
Frame = +2
Query: 248 YTFDEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMMFIFKVVLP 424
++ D F+Y +++ E++ Q+ I YC C S I Y++HS S+ +++I ++P
Sbjct: 128 FSVDAFMYLEEDIEELVQRGCIAREYCRTCGSIAIGLCDYITHSFSQDQLVYISCYLIP 186
>UniRef50_Q387W6 Cluster: Putative uncharacterized protein; n=1;
Trypanosoma brucei|Rep: Putative uncharacterized protein
- Trypanosoma brucei
Length = 481
Score = 36.3 bits (80), Expect(2) = 0.11
Identities = 16/56 (28%), Positives = 30/56 (53%)
Frame = +2
Query: 257 DEFLYDKDEFEDMTQKKKIQLHYCVDCNSRNIKELIYVSHSMSKSVMMFIFKVVLP 424
D FLY +++ + + + I YC C S +I +++HS S ++F+ +LP
Sbjct: 161 DAFLYLEEDIDALADQGHISREYCRSCGSVDIGLCQFITHSFSLEQLIFLSCFLLP 216
Score = 21.8 bits (44), Expect(2) = 0.11
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = +2
Query: 449 IDIGSRLGSVLYGAYYLS 502
+++GSRLG V Y+ S
Sbjct: 251 VEVGSRLGIVPMSCYFAS 268
>UniRef50_UPI000051A210 Cluster: PREDICTED: similar to CG32796-PB,
isoform B, partial; n=2; Apocrita|Rep: PREDICTED:
similar to CG32796-PB, isoform B, partial - Apis
mellifera
Length = 870
Score = 35.1 bits (77), Expect = 1.7
Identities = 22/59 (37%), Positives = 33/59 (55%), Gaps = 1/59 (1%)
Frame = +3
Query: 48 INIEQWINSRAYKK-DLEEKRSLMKSEDILRNIGDAIKKKAPFEAVMPSEKISPPTVGD 221
I I+ N +K+ L+EK K +I + I D IK + PF+ + PSE I PP +G+
Sbjct: 817 IKIQSVSNDNCHKECSLQEK----KLNEIKKIIID-IKNRIPFDGIFPSEHIVPPIIGE 870
>UniRef50_UPI0000EBC6A7 Cluster: PREDICTED: hypothetical protein;
n=3; Bos taurus|Rep: PREDICTED: hypothetical protein -
Bos taurus
Length = 1204
Score = 32.7 bits (71), Expect = 9.2
Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 1/79 (1%)
Frame = +3
Query: 57 EQWINSRAYKKDLEEKRSLMKSEDILRNIGDAIKKKAPFEAVMPSEKISPPTVGD-LADC 233
E+++ S+A L + K E N+ + +K PFEA+ P+EK PT+ + L C
Sbjct: 711 EKFLISQATANPLANQLQKYKCEPCKDNVESVLGEKLPFEALRPAEK---PTLDERLRTC 767
Query: 234 TDSNTIHLMSFYMIRMNLK 290
N +R L+
Sbjct: 768 DTLNRSQARELTQLRQTLQ 786
>UniRef50_Q8WXH0 Cluster: Nesprin-2; n=34; Eutheria|Rep: Nesprin-2 -
Homo sapiens (Human)
Length = 6885
Score = 32.7 bits (71), Expect = 9.2
Identities = 25/83 (30%), Positives = 42/83 (50%)
Frame = +3
Query: 66 INSRAYKKDLEEKRSLMKSEDILRNIGDAIKKKAPFEAVMPSEKISPPTVGDLADCTDSN 245
+NS K LEE+ +K +L+ GDA + +A + T+ D+++ D N
Sbjct: 2067 LNSSEGKMPLEERIQKIKEIILLKPEGDARIETIMKQAESSEAPLVQKTLTDISNQWD-N 2125
Query: 246 TIHLMSFYMIRMNLKI*LKRKKY 314
T+HL S Y+ K+ L+ +KY
Sbjct: 2126 TLHLASTYLSHQE-KLLLEGEKY 2147
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 626,110,469
Number of Sequences: 1657284
Number of extensions: 12169426
Number of successful extensions: 35191
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 33730
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35161
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 57024798702
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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