Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0369
(690 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 56 2e-08
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 56 3e-08
At3g11110.1 68416.m01345 zinc finger (C3HC4-type RING finger) fa... 31 0.95
At5g06839.1 68418.m00773 bZIP family transcription factor contai... 29 2.2
At4g01100.1 68417.m00148 mitochondrial substrate carrier family ... 29 2.2
At3g01850.2 68416.m00129 ribulose-phosphate 3-epimerase, cytosol... 29 3.8
At3g01850.1 68416.m00128 ribulose-phosphate 3-epimerase, cytosol... 29 3.8
At1g64310.1 68414.m07288 pentatricopeptide (PPR) repeat-containi... 28 5.1
At1g71980.1 68414.m08320 protease-associated zinc finger (C3HC4-... 28 6.7
At1g30100.1 68414.m03679 9-cis-epoxycarotenoid dioxygenase, puta... 28 6.7
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g... 27 8.9
At4g02560.1 68417.m00350 homeobox protein LUMINIDEPENDENS (LD) i... 27 8.9
At3g51130.1 68416.m05599 expressed protein contains Pfam PF03676... 27 8.9
At3g17830.1 68416.m02273 DNAJ heat shock family protein similar ... 27 8.9
At1g19130.1 68414.m02379 expressed protein 27 8.9
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 56.0 bits (129), Expect = 2e-08
Identities = 32/69 (46%), Positives = 41/69 (59%)
Frame = +2
Query: 53 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 232
MS K EKP L G RIKTRKR+ DP F DA+VQ GDL+ K ++S S L
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIES--SDL 57
Query: 233 DYRRYGEAY 259
++ RYG+ +
Sbjct: 58 NFTRYGDIF 66
Score = 31.5 bits (68), Expect = 0.54
Identities = 22/83 (26%), Positives = 37/83 (44%)
Frame = +1
Query: 259 FDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMF 438
F+V+ GG PG S +GE + + + + K++RR +L K
Sbjct: 67 FEVIFIGGRTQPGTVKSDEGE--RHTYSVIDCEPKREAILPSVVYIQKILRRKPFLIKNL 124
Query: 439 EEEMKKVLVYLKGFDPEQRIKLA 507
E ++ L L+ F+ +R KLA
Sbjct: 125 ENVTRRFLQSLELFEENERKKLA 147
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 55.6 bits (128), Expect = 3e-08
Identities = 33/69 (47%), Positives = 41/69 (59%)
Frame = +2
Query: 53 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 232
MS K EKP L G RIKTRKR+ DP F DALVQ GDL+ + L+S S L
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLES--SDL 57
Query: 233 DYRRYGEAY 259
++ RYG+ +
Sbjct: 58 NFSRYGDIF 66
Score = 28.3 bits (60), Expect = 5.1
Identities = 22/83 (26%), Positives = 36/83 (43%)
Frame = +1
Query: 259 FDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMF 438
F+V+ GG G S +GE + I + + K++RR +L K
Sbjct: 67 FEVVFIGGRTQTGSVKSDEGERHPYS--IIDCEPKREAILPSVVYIQKILRRKAFLIKNL 124
Query: 439 EEEMKKVLVYLKGFDPEQRIKLA 507
E ++ L L+ F+ +R KLA
Sbjct: 125 ENVTRRFLQSLELFEENERKKLA 147
>At3g11110.1 68416.m01345 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 Zinc
finger, C3HC4 type (RING finger)
Length = 158
Score = 30.7 bits (66), Expect = 0.95
Identities = 26/79 (32%), Positives = 38/79 (48%), Gaps = 1/79 (1%)
Frame = +1
Query: 289 LPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMFEEEMKKVLVY 468
LP S+++ SP T S D +R+ V L RR E+ EEE K+ +
Sbjct: 58 LPRDSINLHASSPDRLTRCRSGGLDPAEIRSLPVV---LCRR----ERAEEEEEKECCIC 110
Query: 469 LKGFDPEQRIK-LAP*LHC 522
L GF+ +++K L P HC
Sbjct: 111 LGGFEEGEKMKVLPPCSHC 129
>At5g06839.1 68418.m00773 bZIP family transcription factor contains
Pfam profile: PF00170 bZIP transcription factor
Length = 417
Score = 29.5 bits (63), Expect = 2.2
Identities = 17/63 (26%), Positives = 25/63 (39%), Gaps = 3/63 (4%)
Frame = -2
Query: 293 GSSRPPAMSTSNMLRHSVGSRVLIRPSLGTCRL--RRDRH-RPAPDPVPERRGNRSGHTS 123
G PP+ + S + H + +RP T + + H P P + GNR G S
Sbjct: 47 GHHDPPSTAPSPLHHHHTTQNLAMRPPTSTLNIFPSQPMHIEPPPSSTHNKEGNRKGLAS 106
Query: 122 LSH 114
H
Sbjct: 107 SDH 109
>At4g01100.1 68417.m00148 mitochondrial substrate carrier family
protein contains Pfam profile: PF00153 mitochondrial
carrier protein
Length = 352
Score = 29.5 bits (63), Expect = 2.2
Identities = 18/70 (25%), Positives = 35/70 (50%), Gaps = 2/70 (2%)
Frame = +3
Query: 405 DASVQVLGKNVRRGNEKGSGLLERLRSRTAHQA-GAMTALWIGNGC-MPPSVLLVLVNEH 578
DAS V G+ + + +G+++ R H+ GA+ + N + PS+ + V
Sbjct: 279 DASAIVTGEGRSTASLEYTGMVDAFRKTVRHEGFGALYKGLVPNSVKVVPSIAIAFVTYE 338
Query: 579 LLKDNLALDF 608
++KD L ++F
Sbjct: 339 MVKDVLGVEF 348
>At3g01850.2 68416.m00129 ribulose-phosphate 3-epimerase, cytosolic,
putative / pentose-5-phosphate 3-epimerase, putative
strong similarity to D-ribulose-5-phosphate 3-epimerase
[Oryza sativa] GI:6007803; contains Pfam profile
PF00834: Ribulose-phosphate 3 epimerase family; contains
non-consensus splice sites at exon 1 and exon2
Length = 225
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = -3
Query: 550 DGGMHPLPIHSAVMAPA*CAVRDRSLSSRPEP 455
DGG+ P I +A A A C V S+ PEP
Sbjct: 176 DGGLGPSTIDTAAAAGANCIVAGSSVFGAPEP 207
>At3g01850.1 68416.m00128 ribulose-phosphate 3-epimerase, cytosolic,
putative / pentose-5-phosphate 3-epimerase, putative
strong similarity to D-ribulose-5-phosphate 3-epimerase
[Oryza sativa] GI:6007803; contains Pfam profile
PF00834: Ribulose-phosphate 3 epimerase family; contains
non-consensus splice sites at exon 1 and exon2
Length = 225
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = -3
Query: 550 DGGMHPLPIHSAVMAPA*CAVRDRSLSSRPEP 455
DGG+ P I +A A A C V S+ PEP
Sbjct: 176 DGGLGPSTIDTAAAAGANCIVAGSSVFGAPEP 207
>At1g64310.1 68414.m07288 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 552
Score = 28.3 bits (60), Expect = 5.1
Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 2/64 (3%)
Frame = +3
Query: 411 SVQVLGKNVRRGNEKGSGLLERLRSRTAHQAGAMTALWIGNGCMPPSVLLVLVNEH--LL 584
+V +LG +KG L ++ R HQ T + + +G + PS+LLV + H L
Sbjct: 176 NVMILGYGCCGFWDKGINLFNLMQHR-GHQPNCYTMVALTSGLIDPSLLLVAWSVHAFCL 234
Query: 585 KDNL 596
K NL
Sbjct: 235 KINL 238
>At1g71980.1 68414.m08320 protease-associated zinc finger
(C3HC4-type RING finger) family protein identical to
ReMembR-H2 protein JR702 [Arabidopsis thaliana]
gi|6942149|gb|AAF32326; contains Pfam domain, PF02225:
protease-associated (PA) domain and Pfam domain,
PF00097: Zinc finger, C3HC4 type (RING finger);
identical to cDNA ReMembR-H2 protein JR702 mRNA, partial
cds GI:6942148
Length = 448
Score = 27.9 bits (59), Expect = 6.7
Identities = 24/87 (27%), Positives = 38/87 (43%), Gaps = 3/87 (3%)
Frame = -2
Query: 398 TNTCSKFRMVSISSLAEKMQVLVLGDSPSIDTEPPGSSRPPAMSTSNMLRHSVGSRVLIR 219
++ S F S+ S +L+ PS+ + P S PA ++S+ +R S S R
Sbjct: 294 SSAASSFTSSSLHSSVRSSALLI---GPSLGSLPTSISFSPAYASSSYIRQSFQSSSNRR 350
Query: 218 -PSLGTCRLRRDRHRPA--PDPVPERR 147
P + R D + A P P P +R
Sbjct: 351 SPPISVSRSSVDLRQQAASPSPSPSQR 377
>At1g30100.1 68414.m03679 9-cis-epoxycarotenoid dioxygenase,
putative / neoxanthin cleavage enzyme, putative /
carotenoid cleavage dioxygenase, putative similar to
9-cis-epoxycarotenoid dioxygenase GI:6715257 from
[Phaseolus vulgaris]
Length = 589
Score = 27.9 bits (59), Expect = 6.7
Identities = 24/86 (27%), Positives = 40/86 (46%)
Frame = -2
Query: 407 ISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSIDTEPPGSSRPPAMSTSNMLRHSVGSRV 228
+SFTNT + R +S +S+++ +L + PS + P S N L+ + + +
Sbjct: 32 VSFTNTKPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEKD----TSRWNPLQRAASAAL 87
Query: 227 LIRPSLGTCRLRRDRHRPAPDPVPER 150
T LRR+R +P P V R
Sbjct: 88 ---DFAETALLRRERSKPLPKTVDPR 110
>At4g25000.1 68417.m03587 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
GI:21670851; contains Pfam profile PF00128: Alpha
amylase, catalytic domain
Length = 423
Score = 27.5 bits (58), Expect = 8.9
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 113 DEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 208
D K YD N R L Q +E AGG + A+ +
Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDF 269
>At4g02560.1 68417.m00350 homeobox protein LUMINIDEPENDENS (LD)
identical to Homeobox protein LUMINIDEPENDENS
(Swiss-Prot:Q38796) [Arabidopsis thaliana]
Length = 953
Score = 27.5 bits (58), Expect = 8.9
Identities = 21/61 (34%), Positives = 30/61 (49%), Gaps = 2/61 (3%)
Frame = +2
Query: 326 PRPTPASSPPMRIWTPCEISNRYS*N*CVGTSTWKKCSK-RK*KRFW-ST*KASIPNSAS 499
P PTP+++P M W I N +S VGT+ + ++ R W T + SIP A
Sbjct: 753 PSPTPSTNPGMSGWGQEGIRNPFSRQNQVGTAVARSGTQLRVGSMQWHQTNEQSIPRHAP 812
Query: 500 S 502
S
Sbjct: 813 S 813
>At3g51130.1 68416.m05599 expressed protein contains Pfam PF03676:
Uncharacterised protein family (UPF0183)
Length = 410
Score = 27.5 bits (58), Expect = 8.9
Identities = 25/84 (29%), Positives = 35/84 (41%)
Frame = -1
Query: 510 WRQLDALFGIEAFQVDQNLFHFLFEHFFQVLVPTHQFYEYLFEISHGVHILIGGEDAGVG 331
W +L GI QVDQ + H + + + FY Y + G+ IL GE V
Sbjct: 254 WTELGRPCGIHPKQVDQMVIHSASDPRPKTTICGDYFYNY---FTRGLDILFDGETHKV- 309
Query: 330 LGRFAVHRHRTARQQQAASNEYIE 259
+F +H T A N YI+
Sbjct: 310 -KKFVLH---TNYPGHADFNSYIK 329
>At3g17830.1 68416.m02273 DNAJ heat shock family protein similar to
SP|P35514 Chaperone protein dnaJ {Lactococcus lactis};
contains Pfam profiles PF00226: DnaJ domain, PF01556:
DnaJ C terminal region, PF00684: DnaJ central domain (4
repeats)
Length = 517
Score = 27.5 bits (58), Expect = 8.9
Identities = 18/56 (32%), Positives = 26/56 (46%)
Frame = +2
Query: 89 QRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKLDYRRYGEA 256
Q IK+ R KY P+ ++ G E + AAY+ L + Y R+GEA
Sbjct: 77 QEIKSSYRKLARKYHPDMNKNP---GAEDKFKQISAAYEVLSDEEKRSAYDRFGEA 129
>At1g19130.1 68414.m02379 expressed protein
Length = 187
Score = 27.5 bits (58), Expect = 8.9
Identities = 15/51 (29%), Positives = 24/51 (47%)
Frame = +1
Query: 286 LLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMF 438
LLP GSVS P T F E + +R+ ++ ++L++MF
Sbjct: 59 LLPSGSVSRLHRIPMAETWHFYLGEPLTVIRSLSTRSLQTSGLVRFLQRMF 109
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,688,088
Number of Sequences: 28952
Number of extensions: 364489
Number of successful extensions: 1421
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1314
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1419
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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