Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0354
(707 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g38070.1 68417.m05377 bHLH family protein contains Pfam profi... 35 0.061
At1g06530.1 68414.m00692 myosin heavy chain-related similar to m... 34 0.080
At3g53350.3 68416.m05888 myosin heavy chain-related low similari... 31 0.75
At3g53350.2 68416.m05887 myosin heavy chain-related low similari... 31 0.75
At3g53350.1 68416.m05886 myosin heavy chain-related low similari... 31 0.75
At4g32700.1 68417.m04655 DNA-directed DNA polymerase family prot... 30 1.3
At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ... 30 1.7
At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ... 30 1.7
At2g46180.1 68415.m05742 intracellular protein transport protein... 30 1.7
At1g34320.1 68414.m04259 expressed protein contains Pfam domain ... 30 1.7
At3g30560.1 68416.m03867 hypothetical protein 29 2.3
At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 29 2.3
At2g38720.1 68415.m04755 microtubule associated protein (MAP65/A... 29 2.3
At1g19230.1 68414.m02393 respiratory burst oxidase protein E (Rb... 29 3.0
At1g02330.1 68414.m00178 expressed protein contains similarity t... 29 3.0
At1g64170.1 68414.m07269 cation/hydrogen exchanger, putative (CH... 28 5.3
At5g60360.1 68418.m07568 cysteine proteinase, putative / AALP pr... 28 7.0
At1g33970.1 68414.m04212 avirulence-responsive protein, putative... 28 7.0
At5g46860.1 68418.m05774 syntaxin 22 (SYP22) (VAM3) identical to... 27 9.2
At1g27595.1 68414.m03365 expressed protein similar to Symplekin ... 27 9.2
>At4g38070.1 68417.m05377 bHLH family protein contains Pfam profile:
PF00010 helix-loop-helix DNA-binding domain; PMID:
12679534; putative bHLH131 transcription factor
Length = 1513
Score = 34.7 bits (76), Expect = 0.061
Identities = 16/36 (44%), Positives = 24/36 (66%)
Frame = +2
Query: 296 RAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKL 403
+ EW E +++ DEI +L+T L S R S DL++KL
Sbjct: 211 KKEWEEEKSKLLDEIYSLQTKLDSVTRISEDLQKKL 246
>At1g06530.1 68414.m00692 myosin heavy chain-related similar to
myosin heavy chain (GI:1408194) {Placopecten
magellanicus}; similar to Myosin heavy chain, clone 203
(Fragment) (SP:P39922){Hydra attenuata}; contains one
transmembrane domain
Length = 323
Score = 34.3 bits (75), Expect = 0.080
Identities = 19/54 (35%), Positives = 34/54 (62%), Gaps = 3/54 (5%)
Frame = +2
Query: 281 QADQLRAEWSRE---LARVEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITE 433
+A++LR+E S++ + +E E+A LRTV + ++ +L+ KLG KE+ E
Sbjct: 131 EAEKLRSEISQKGGGIEELEKEVAGLRTVKEENEKRMKELESKLGALEVKELDE 184
>At3g53350.3 68416.m05888 myosin heavy chain-related low similarity
to filamin-interacting protein S-FILIP [Rattus
norvegicus] GI:21392397, nonmuscle heavy chain myosin
II-A [Mus musculus] GI:17978023
Length = 394
Score = 31.1 bits (67), Expect = 0.75
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = +2
Query: 251 SYELAGLTPEQADQLRAEWSRELARVEDEIATLRTVLQSKIRQ---SSDLKR 397
+YE + Q AE + EL R +DEI LR L K+++ + DLK+
Sbjct: 252 AYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKVKEDESTGDLKK 303
>At3g53350.2 68416.m05887 myosin heavy chain-related low similarity
to filamin-interacting protein S-FILIP [Rattus
norvegicus] GI:21392397, nonmuscle heavy chain myosin
II-A [Mus musculus] GI:17978023
Length = 394
Score = 31.1 bits (67), Expect = 0.75
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = +2
Query: 251 SYELAGLTPEQADQLRAEWSRELARVEDEIATLRTVLQSKIRQ---SSDLKR 397
+YE + Q AE + EL R +DEI LR L K+++ + DLK+
Sbjct: 252 AYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKVKEDESTGDLKK 303
>At3g53350.1 68416.m05886 myosin heavy chain-related low similarity
to filamin-interacting protein S-FILIP [Rattus
norvegicus] GI:21392397, nonmuscle heavy chain myosin
II-A [Mus musculus] GI:17978023
Length = 396
Score = 31.1 bits (67), Expect = 0.75
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = +2
Query: 251 SYELAGLTPEQADQLRAEWSRELARVEDEIATLRTVLQSKIRQ---SSDLKR 397
+YE + Q AE + EL R +DEI LR L K+++ + DLK+
Sbjct: 254 AYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKVKEDESTGDLKK 305
>At4g32700.1 68417.m04655 DNA-directed DNA polymerase family protein
similar to DNA helicase HEL308 [Homo sapiens]
GI:19110782; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain, PF00476: DNA polymerase family A
Length = 1548
Score = 30.3 bits (65), Expect = 1.3
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +1
Query: 502 NDPEKTSSIIGGITAGVSSKLGQMRNSESFRSIEERVGS 618
N EK S ++G T GVSS++ +FR I VG+
Sbjct: 799 NHREKPSEVLGDATLGVSSEINLTSRLPNFRPIGTAVGT 837
>At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 765
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +2
Query: 278 EQADQLRAEWSRELARVEDEIATLRTVLQSK 370
E AD+L E RE++R+ DE+ LR ++SK
Sbjct: 585 ELADRLIEEKDREISRLVDEMTNLRKSMESK 615
>At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 788
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +2
Query: 278 EQADQLRAEWSRELARVEDEIATLRTVLQSK 370
E AD+L E RE++R+ DE+ LR ++SK
Sbjct: 585 ELADRLIEEKDREISRLVDEMTNLRKSMESK 615
>At2g46180.1 68415.m05742 intracellular protein transport protein
USO1-related similar to Intracellular protein transport
protein USO1 (Swiss-Prot:P25386) [Saccharomyces
cerevisiae]
Length = 725
Score = 29.9 bits (64), Expect = 1.7
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = +2
Query: 284 ADQLRAEWSRELARVEDEIATLRTVLQSKI 373
A+ + AEW +++VED+ A +R VL+ +
Sbjct: 528 AENIAAEWKNRVSKVEDDNAKVRRVLEQSM 557
>At1g34320.1 68414.m04259 expressed protein contains Pfam domain
PF05003: protein of unknown function (DUF668)
Length = 657
Score = 29.9 bits (64), Expect = 1.7
Identities = 17/45 (37%), Positives = 28/45 (62%)
Frame = +2
Query: 326 VEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGLKNV 460
V D +A LRT L+S+ + +LK+K ++W I E+V + L +V
Sbjct: 308 VGDTLAILRTELKSQKKHVRNLKKK---SLWSRILEEVMEKLVDV 349
>At3g30560.1 68416.m03867 hypothetical protein
Length = 1473
Score = 29.5 bits (63), Expect = 2.3
Identities = 12/39 (30%), Positives = 26/39 (66%)
Frame = +2
Query: 584 SLSGPSKNASVRLMKTSRGKVASRSNSTQSFDEALRDAS 700
++SG S +++RLM ++ KV++ + ++ FD+ + D S
Sbjct: 36 NISGESIVSTIRLMSNNKEKVSNDQDRSEEFDDHIFDCS 74
>At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam
profile: PF00225 Kinesin motor domain; contains
non-consensus splice site (GC) at intron 12
Length = 2722
Score = 29.5 bits (63), Expect = 2.3
Identities = 19/60 (31%), Positives = 31/60 (51%)
Frame = +2
Query: 290 QLRAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGLKNVKES 469
Q+R+E ELA I L TV+ ++ ++ L KL +T D+N+ L VK++
Sbjct: 2415 QMRSEKDEELAAARLRIEELETVVSTRQKEIFLLNSKLAKV--DSMTHDINRVLLGVKQN 2472
>At2g38720.1 68415.m04755 microtubule associated protein
(MAP65/ASE1) family protein low similarity to myosin
[Schistosoma japonicum] GI:3941320; contains Pfam
profile PF03999: Microtubule associated protein
(MAP65/ASE1 family)
Length = 587
Score = 29.5 bits (63), Expect = 2.3
Identities = 19/56 (33%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Frame = +2
Query: 278 EQADQLRAEWSRELARVEDEIATLRTVLQSKI--RQSSDLKRKLGITVWKEITEDV 439
E+ + RAE R LA+ E EIA+L + L K+ + + K I+ K + ED+
Sbjct: 50 EKTRKFRAELQRSLAQAEAEIASLMSALGEKVSFAKKKEGSLKEQISSVKPVLEDL 105
>At1g19230.1 68414.m02393 respiratory burst oxidase protein E
(RbohE) / NADPH oxidase nearly identical to respiratory
burst oxidase protein E GI:3242787 [gi:3242787] from
[Arabidopsis thaliana]
Length = 926
Score = 29.1 bits (62), Expect = 3.0
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = +1
Query: 148 HSFHTVRVFHCKI*AVFKQLKKQCQ 222
H TV VF+C I V K+LKKQ Q
Sbjct: 885 HPNSTVGVFYCGIQTVAKELKKQAQ 909
>At1g02330.1 68414.m00178 expressed protein contains similarity to
hepatocellular carcinoma-associated antigen 59
GI:7158847 from [Homo sapiens]
Length = 279
Score = 29.1 bits (62), Expect = 3.0
Identities = 19/61 (31%), Positives = 32/61 (52%)
Frame = +2
Query: 302 EWSRELARVEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGLKNVKESQVYQ 481
E EL RVEDE+ + L+ K ++SS+ T E+ + LKN++E++ +
Sbjct: 122 EVENELKRVEDELYKIPDHLKVK-KRSSEESSTQWTTGIAEVQLPIEYKLKNIEETEAAK 180
Query: 482 K 484
K
Sbjct: 181 K 181
>At1g64170.1 68414.m07269 cation/hydrogen exchanger, putative
(CHX16) monovalent cation:proton antiporter family 2
(CPA2) member, PMID:11500563
Length = 847
Score = 28.3 bits (60), Expect = 5.3
Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = -1
Query: 404 QVYVLSPSFD-GSCFAVQFSKSRFHLRHGLARDSIQRVTG 288
++ V PS G ++ +F+K L++ L RDS+Q +TG
Sbjct: 71 EIIVSPPSTGLGQSYSFRFNKYPTRLKYELYRDSVQLITG 110
>At5g60360.1 68418.m07568 cysteine proteinase, putative / AALP
protein (AALP) identical to AALP protein GI:7230640 from
[Arabidopsis thaliana]; similar to barley aleurain
Length = 358
Score = 27.9 bits (59), Expect = 7.0
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +1
Query: 496 DKNDPEK-TSSIIGGITAGVSSKLGQMRNSESFRSIEERVGSAYENVK 636
D+++P + S + + VS LGQ R+ SF R G Y+NV+
Sbjct: 27 DESNPIRMVSDGLREVEESVSQILGQSRHVLSFARFTHRYGKKYQNVE 74
>At1g33970.1 68414.m04212 avirulence-responsive protein, putative /
avirulence induced gene protein, putative / AIG protein,
putative similar to SP|P54120 AIG1 protein {Arabidopsis
thaliana}; contains Pfam profile PF04548: AIG1 family
Length = 342
Score = 27.9 bits (59), Expect = 7.0
Identities = 19/48 (39%), Positives = 29/48 (60%), Gaps = 2/48 (4%)
Frame = +2
Query: 269 LTPEQADQLRAEW-SRELA-RVEDEIATLRTVLQSKIRQSSDLKRKLG 406
L EQA +L AE + E+ R DEI LR L+ +++ +L++KLG
Sbjct: 290 LGEEQAARLEAEKRANEVQKRSSDEIKKLRENLERAEKETKELQKKLG 337
>At5g46860.1 68418.m05774 syntaxin 22 (SYP22) (VAM3) identical to
GP|8809669| syntaxin related protein AtVam3p
[Arabidopsis thaliana]
Length = 268
Score = 27.5 bits (58), Expect = 9.2
Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 3/62 (4%)
Frame = +2
Query: 275 PEQADQLRAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKLGIT--VWKEITEDVN-Q 445
PEQ QL+ +EL +++EIA V++ + + ++ +++G ++K++ VN Q
Sbjct: 150 PEQRAQLQESKRQELVLLDNEIAFNEAVIEEREQGIQEIHQQIGEVNEIFKDLAVLVNDQ 209
Query: 446 GL 451
G+
Sbjct: 210 GV 211
>At1g27595.1 68414.m03365 expressed protein similar to Symplekin
(SP:Q92797) {Homo sapiens}
Length = 1091
Score = 27.5 bits (58), Expect = 9.2
Identities = 14/46 (30%), Positives = 28/46 (60%)
Frame = +2
Query: 254 YELAGLTPEQADQLRAEWSRELARVEDEIATLRTVLQSKIRQSSDL 391
+E+ G P+ +Q+ ++ELA D IAT++ + ++K++ S L
Sbjct: 816 FEVYGRAPKMVNQVLQILTQELAPSLDLIATVKHLYETKLKDVSIL 861
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,141,779
Number of Sequences: 28952
Number of extensions: 276507
Number of successful extensions: 769
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 769
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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