BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0352 (734 letters) Database: celegans 27,780 sequences; 12,740,198 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AL117195-4|CAB55010.1| 571|Caenorhabditis elegans Hypothetical ... 32 0.48 Z81460-5|CAB03832.1| 952|Caenorhabditis elegans Hypothetical pr... 31 1.1 Z70209-3|CAA94147.1| 952|Caenorhabditis elegans Hypothetical pr... 31 1.1 U39653-1|AAM69065.2| 2471|Caenorhabditis elegans Hypothetical pr... 30 1.5 AF003137-3|AAB93640.1| 625|Caenorhabditis elegans Hypothetical ... 30 1.5 Z73424-3|CAA97781.2| 1150|Caenorhabditis elegans Hypothetical pr... 30 2.0 U55374-8|AAB36868.3| 1538|Caenorhabditis elegans Uncoordinated p... 29 2.6 U55374-6|AAM69092.1| 1926|Caenorhabditis elegans Uncoordinated p... 29 2.6 AF078790-10|AAC26931.1| 197|Caenorhabditis elegans Hypothetical... 29 2.6 Z72517-3|CAA96695.1| 717|Caenorhabditis elegans Hypothetical pr... 29 3.4 U28738-2|AAA68309.4| 982|Caenorhabditis elegans Hypothetical pr... 29 4.5 Z81114-2|CAB03286.1| 604|Caenorhabditis elegans Hypothetical pr... 28 6.0 Z68104-8|CAA92117.1| 1077|Caenorhabditis elegans Hypothetical pr... 28 6.0 Z66563-4|CAA91470.1| 1077|Caenorhabditis elegans Hypothetical pr... 28 6.0 Z46242-15|CAA86337.2| 2507|Caenorhabditis elegans Hypothetical p... 28 6.0 Z35598-8|CAA84657.2| 2507|Caenorhabditis elegans Hypothetical pr... 28 6.0 U97003-7|AAB52274.1| 357|Caenorhabditis elegans Hypothetical pr... 28 6.0 AF435953-1|AAL30829.1| 1077|Caenorhabditis elegans eIF2 alpha ki... 28 6.0 AF193341-1|AAF61201.1| 1077|Caenorhabditis elegans eukaryotic tr... 28 6.0 AC025727-4|AAG23388.3| 463|Caenorhabditis elegans Hypothetical ... 28 6.0 Z92831-2|CAB07368.2| 861|Caenorhabditis elegans Hypothetical pr... 28 7.9 U88169-3|AAK68396.1| 345|Caenorhabditis elegans Hypothetical pr... 28 7.9 U80836-7|AAB37893.3| 451|Caenorhabditis elegans Hypothetical pr... 28 7.9 U70852-1|AAK29815.1| 2361|Caenorhabditis elegans Hypothetical pr... 28 7.9 U56963-8|AAB38121.1| 155|Caenorhabditis elegans Hypothetical pr... 28 7.9 U55373-1|AAC25894.1| 1829|Caenorhabditis elegans Lethal protein ... 28 7.9 U50311-12|AAA92315.1| 169|Caenorhabditis elegans Hypothetical p... 28 7.9 AL021447-3|CAA16274.1| 459|Caenorhabditis elegans Hypothetical ... 28 7.9 AF308445-1|AAG29838.1| 1829|Caenorhabditis elegans LET-418 protein. 28 7.9 AC006833-6|AAF60950.1| 491|Caenorhabditis elegans Hypothetical ... 28 7.9 AC006731-1|AAF60483.1| 4900|Caenorhabditis elegans Temporarily a... 28 7.9 >AL117195-4|CAB55010.1| 571|Caenorhabditis elegans Hypothetical protein Y57A10A.5 protein. Length = 571 Score = 31.9 bits (69), Expect = 0.48 Identities = 18/65 (27%), Positives = 27/65 (41%) Frame = +2 Query: 134 PAPEKPASSDENVQFPPLPEPTDEDFQDDLNEEEKEYIKTNLRVSNSSLKSCENYFRNIK 313 P PE P + V+ P PEP D +F N + + N N + + + IK Sbjct: 353 PIPEDPPYESDEVEGQPKPEPIDFEFDQTNNRDVSHSLNINNLRENGEVSNNPS-ISEIK 411 Query: 314 IMFCF 328 + F F Sbjct: 412 VDFPF 416 >Z81460-5|CAB03832.1| 952|Caenorhabditis elegans Hypothetical protein C04A11.4 protein. Length = 952 Score = 30.7 bits (66), Expect = 1.1 Identities = 16/44 (36%), Positives = 24/44 (54%) Frame = +2 Query: 101 MSEIIKSEGACPAPEKPASSDENVQFPPLPEPTDEDFQDDLNEE 232 ++E +S+ A + S NVQ PP+P P+D D LNE+ Sbjct: 818 VAESFRSDSAASFNTRTGSFRPNVQPPPVPRPSD-DVLSKLNED 860 >Z70209-3|CAA94147.1| 952|Caenorhabditis elegans Hypothetical protein C04A11.4 protein. Length = 952 Score = 30.7 bits (66), Expect = 1.1 Identities = 16/44 (36%), Positives = 24/44 (54%) Frame = +2 Query: 101 MSEIIKSEGACPAPEKPASSDENVQFPPLPEPTDEDFQDDLNEE 232 ++E +S+ A + S NVQ PP+P P+D D LNE+ Sbjct: 818 VAESFRSDSAASFNTRTGSFRPNVQPPPVPRPSD-DVLSKLNED 860 >U39653-1|AAM69065.2| 2471|Caenorhabditis elegans Hypothetical protein T13H2.5a protein. Length = 2471 Score = 30.3 bits (65), Expect = 1.5 Identities = 15/51 (29%), Positives = 29/51 (56%) Frame = +2 Query: 122 EGACPAPEKPASSDENVQFPPLPEPTDEDFQDDLNEEEKEYIKTNLRVSNS 274 EG A EK + + FP + +DE+ Q DL++EE+ + ++ +S++ Sbjct: 321 EGTSVAAEKEVLEEGEMDFPIEIKSSDEE-QTDLDDEEESMLDSDFEISDN 370 >AF003137-3|AAB93640.1| 625|Caenorhabditis elegans Hypothetical protein C27A12.2 protein. Length = 625 Score = 30.3 bits (65), Expect = 1.5 Identities = 11/42 (26%), Positives = 22/42 (52%) Frame = +3 Query: 348 SQRLHCTACDRHLGSSSRNLSRVKIHPLLRTLVCQTCHIFYN 473 +Q HC C++H+ S+ ++ H + VC C+I ++ Sbjct: 68 AQIYHCVQCNKHIKYPSKITEHIRKHTGEKPNVCSICNISFS 109 >Z73424-3|CAA97781.2| 1150|Caenorhabditis elegans Hypothetical protein C44B9.4 protein. Length = 1150 Score = 29.9 bits (64), Expect = 2.0 Identities = 9/20 (45%), Positives = 10/20 (50%) Frame = +2 Query: 515 CRWCGQGGQVFCCSDCPHVF 574 C C GG + CC CP F Sbjct: 62 CGICSSGGNILCCEQCPASF 81 >U55374-8|AAB36868.3| 1538|Caenorhabditis elegans Uncoordinated protein 2, isoform a protein. Length = 1538 Score = 29.5 bits (63), Expect = 2.6 Identities = 12/40 (30%), Positives = 22/40 (55%) Frame = +2 Query: 197 TDEDFQDDLNEEEKEYIKTNLRVSNSSLKSCENYFRNIKI 316 T+EDF++D +E E+EY+ R S + + ++I Sbjct: 341 TEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRI 380 >U55374-6|AAM69092.1| 1926|Caenorhabditis elegans Uncoordinated protein 2, isoform c protein. Length = 1926 Score = 29.5 bits (63), Expect = 2.6 Identities = 12/40 (30%), Positives = 22/40 (55%) Frame = +2 Query: 197 TDEDFQDDLNEEEKEYIKTNLRVSNSSLKSCENYFRNIKI 316 T+EDF++D +E E+EY+ R S + + ++I Sbjct: 341 TEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRI 380 >AF078790-10|AAC26931.1| 197|Caenorhabditis elegans Hypothetical protein F36H12.5 protein. Length = 197 Score = 29.5 bits (63), Expect = 2.6 Identities = 21/73 (28%), Positives = 34/73 (46%), Gaps = 1/73 (1%) Frame = +2 Query: 26 LLQTLSKYKHFYILLMRIPNDLGTTMSEIIKSEGACPAPEKPASSDENVQFPPLPEPTDE 205 ++ + Y F I+LM + +G S+ K + A P P SS+ V P P P E Sbjct: 1 MIHLVCAYHIFAIILMSMTVSVGCK-SKAKKPKAAAPNPISSISSNSKVAPPASPPPPPE 59 Query: 206 -DFQDDLNEEEKE 241 + + + EE K+ Sbjct: 60 AEKKPETGEEPKK 72 >Z72517-3|CAA96695.1| 717|Caenorhabditis elegans Hypothetical protein T28F4.4 protein. Length = 717 Score = 29.1 bits (62), Expect = 3.4 Identities = 18/58 (31%), Positives = 29/58 (50%) Frame = +2 Query: 77 IPNDLGTTMSEIIKSEGACPAPEKPASSDENVQFPPLPEPTDEDFQDDLNEEEKEYIK 250 +P+ + S + S + PAP++ S E + L + E+ DD EEEKE +K Sbjct: 247 VPSPSYSFPSSVASSASSSPAPQR---SSEGI-LDDLSDVESENEDDDKKEEEKEDVK 300 >U28738-2|AAA68309.4| 982|Caenorhabditis elegans Hypothetical protein T28D9.7 protein. Length = 982 Score = 28.7 bits (61), Expect = 4.5 Identities = 16/44 (36%), Positives = 23/44 (52%) Frame = +2 Query: 179 PPLPEPTDEDFQDDLNEEEKEYIKTNLRVSNSSLKSCENYFRNI 310 P L E +ED +DD+ +EE + + VS SCE+ R I Sbjct: 432 PALIEEQEEDDEDDVEQEELDENVSFSTVSGGETFSCEDKSRPI 475 >Z81114-2|CAB03286.1| 604|Caenorhabditis elegans Hypothetical protein T04D3.2 protein. Length = 604 Score = 28.3 bits (60), Expect = 6.0 Identities = 16/49 (32%), Positives = 25/49 (51%) Frame = +2 Query: 101 MSEIIKSEGACPAPEKPASSDENVQFPPLPEPTDEDFQDDLNEEEKEYI 247 M EI K PE P S+ E+ + + DED +DDL+ ++ Y+ Sbjct: 238 MQEIEKGIDVTGNPENPDSAVES-DYDSEEDDEDEDDEDDLDVDDLNYL 285 >Z68104-8|CAA92117.1| 1077|Caenorhabditis elegans Hypothetical protein F46C3.1 protein. Length = 1077 Score = 28.3 bits (60), Expect = 6.0 Identities = 21/78 (26%), Positives = 34/78 (43%), Gaps = 3/78 (3%) Frame = +2 Query: 83 NDLGTTMSEIIKSEGACPAPEKPASSDENVQFPPLPE--PTDEDFQDD-LNEEEKEYIKT 253 +DL TT I + P + + Q P+ TD D +D + +EK+ + Sbjct: 525 SDLSTTPIGAIHRKPLMPIEKSNIETHTQPQIKPVQRLVKTDIDTDEDSFSNDEKKRLLR 584 Query: 254 NLRVSNSSLKSCENYFRN 307 N +S SSL+ + F N Sbjct: 585 NRTISRSSLEGFTSRFAN 602 >Z66563-4|CAA91470.1| 1077|Caenorhabditis elegans Hypothetical protein F46C3.1 protein. Length = 1077 Score = 28.3 bits (60), Expect = 6.0 Identities = 21/78 (26%), Positives = 34/78 (43%), Gaps = 3/78 (3%) Frame = +2 Query: 83 NDLGTTMSEIIKSEGACPAPEKPASSDENVQFPPLPE--PTDEDFQDD-LNEEEKEYIKT 253 +DL TT I + P + + Q P+ TD D +D + +EK+ + Sbjct: 525 SDLSTTPIGAIHRKPLMPIEKSNIETHTQPQIKPVQRLVKTDIDTDEDSFSNDEKKRLLR 584 Query: 254 NLRVSNSSLKSCENYFRN 307 N +S SSL+ + F N Sbjct: 585 NRTISRSSLEGFTSRFAN 602 >Z46242-15|CAA86337.2| 2507|Caenorhabditis elegans Hypothetical protein F10F2.1 protein. Length = 2507 Score = 28.3 bits (60), Expect = 6.0 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Frame = +2 Query: 107 EIIKSEGACPAPEKPASSDENVQ-FPPLPEPTDEDFQDDLNEEEKEYI-KTNLRVSN 271 EI+ P P + S ++ PPLPE + + +DD+ + KT L SN Sbjct: 81 EIVVPSVLAPTPSQEVSPPNAIESLPPLPEGKELELEDDVTSSLPRLLSKTTLIHSN 137 >Z35598-8|CAA84657.2| 2507|Caenorhabditis elegans Hypothetical protein F10F2.1 protein. Length = 2507 Score = 28.3 bits (60), Expect = 6.0 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Frame = +2 Query: 107 EIIKSEGACPAPEKPASSDENVQ-FPPLPEPTDEDFQDDLNEEEKEYI-KTNLRVSN 271 EI+ P P + S ++ PPLPE + + +DD+ + KT L SN Sbjct: 81 EIVVPSVLAPTPSQEVSPPNAIESLPPLPEGKELELEDDVTSSLPRLLSKTTLIHSN 137 >U97003-7|AAB52274.1| 357|Caenorhabditis elegans Hypothetical protein F47C10.4 protein. Length = 357 Score = 28.3 bits (60), Expect = 6.0 Identities = 21/57 (36%), Positives = 29/57 (50%), Gaps = 4/57 (7%) Frame = +3 Query: 576 CAKCIKRNFGQSKILEIK--CVDDWKCFKCNPECLKTPAC--CMLGLCFNIVKQGLK 734 C C K F ++ +L ++ C +CF + CLKTP C C L C VK G+K Sbjct: 44 CDAC-KMFFRRTMLLNVQYVCRRKNQCFDDSFSCLKTPYCRACRLVQC---VKVGMK 96 >AF435953-1|AAL30829.1| 1077|Caenorhabditis elegans eIF2 alpha kinase protein. Length = 1077 Score = 28.3 bits (60), Expect = 6.0 Identities = 21/78 (26%), Positives = 34/78 (43%), Gaps = 3/78 (3%) Frame = +2 Query: 83 NDLGTTMSEIIKSEGACPAPEKPASSDENVQFPPLPE--PTDEDFQDD-LNEEEKEYIKT 253 +DL TT I + P + + Q P+ TD D +D + +EK+ + Sbjct: 525 SDLSTTPIGAIHRKPLMPIEKSNIETHTQPQIKPVQRLVKTDIDTDEDSFSNDEKKRLLR 584 Query: 254 NLRVSNSSLKSCENYFRN 307 N +S SSL+ + F N Sbjct: 585 NRTISRSSLEGFTSRFAN 602 >AF193341-1|AAF61201.1| 1077|Caenorhabditis elegans eukaryotic translation initiationfactor 2 alpha kinase PEK protein. Length = 1077 Score = 28.3 bits (60), Expect = 6.0 Identities = 21/78 (26%), Positives = 34/78 (43%), Gaps = 3/78 (3%) Frame = +2 Query: 83 NDLGTTMSEIIKSEGACPAPEKPASSDENVQFPPLPE--PTDEDFQDD-LNEEEKEYIKT 253 +DL TT I + P + + Q P+ TD D +D + +EK+ + Sbjct: 525 SDLSTTPIGAIHRKPLMPIEKSNIETHTQPQIKPVQRLVKTDIDTDEDSFSNDEKKRLLR 584 Query: 254 NLRVSNSSLKSCENYFRN 307 N +S SSL+ + F N Sbjct: 585 NRTISRSSLEGFTSRFAN 602 >AC025727-4|AAG23388.3| 463|Caenorhabditis elegans Hypothetical protein Y73E7A.2 protein. Length = 463 Score = 28.3 bits (60), Expect = 6.0 Identities = 23/72 (31%), Positives = 33/72 (45%), Gaps = 7/72 (9%) Frame = +2 Query: 101 MSEIIKSEGACPAPEKPASSDENVQFPPL----PEPTDEDFQDDLNEEEKEY---IKTNL 259 +SEI +G E DE + PE DE+ +DL +EE E I TN+ Sbjct: 69 ISEISGLQGNFDKSESEEDEDEEEKIDNFDESEPEIEDEEENEDLEDEEDENPEEIDTNM 128 Query: 260 RVSNSSLKSCEN 295 ++ SLK E+ Sbjct: 129 GMTTLSLKDEED 140 >Z92831-2|CAB07368.2| 861|Caenorhabditis elegans Hypothetical protein F22G12.4 protein. Length = 861 Score = 27.9 bits (59), Expect = 7.9 Identities = 15/49 (30%), Positives = 23/49 (46%), Gaps = 2/49 (4%) Frame = +3 Query: 339 TITSQRLHCTACDRHLGS--SSRNLSRVKIHPLLRTLVCQTCHIFYNSG 479 ++TS++ HC C RH+ S S + K R VC C ++G Sbjct: 808 SLTSRKHHCRHCGRHVCSKCSETTMPIAKYGEEKRVRVCDVCAHVISTG 856 >U88169-3|AAK68396.1| 345|Caenorhabditis elegans Hypothetical protein T03F1.6b protein. Length = 345 Score = 27.9 bits (59), Expect = 7.9 Identities = 22/83 (26%), Positives = 35/83 (42%) Frame = +2 Query: 41 SKYKHFYILLMRIPNDLGTTMSEIIKSEGACPAPEKPASSDENVQFPPLPEPTDEDFQDD 220 S+ + L + N L E +K C E ++ N+Q P DE+FQ D Sbjct: 250 SRRRFLESLTIEFGNSLKAVTEEEMKRVLLCHVDEFHHTTP-NLQHFHCSLPHDENFQID 308 Query: 221 LNEEEKEYIKTNLRVSNSSLKSC 289 L++E + +R + S SC Sbjct: 309 LSQEFLALLNEPVRKTRQSKSSC 331 >U80836-7|AAB37893.3| 451|Caenorhabditis elegans Hypothetical protein B0432.13 protein. Length = 451 Score = 27.9 bits (59), Expect = 7.9 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +2 Query: 221 LNEEEKEYIKTNLRVSNSSLKSCENY 298 ++ EE Y+K L+ S + LK+CE Y Sbjct: 147 ISSEETAYLKRELQESKNRLKTCEFY 172 >U70852-1|AAK29815.1| 2361|Caenorhabditis elegans Hypothetical protein F45E4.4 protein. Length = 2361 Score = 27.9 bits (59), Expect = 7.9 Identities = 12/36 (33%), Positives = 17/36 (47%) Frame = +2 Query: 140 PEKPASSDENVQFPPLPEPTDEDFQDDLNEEEKEYI 247 P S V P P P + F+ DL +EE ++I Sbjct: 811 PATQRSITHKVSLTPPPPPPSKHFEQDLTQEELDHI 846 >U56963-8|AAB38121.1| 155|Caenorhabditis elegans Hypothetical protein T13A10.1 protein. Length = 155 Score = 27.9 bits (59), Expect = 7.9 Identities = 20/72 (27%), Positives = 31/72 (43%) Frame = +2 Query: 47 YKHFYILLMRIPNDLGTTMSEIIKSEGACPAPEKPASSDENVQFPPLPEPTDEDFQDDLN 226 Y F I+LM + +G + K + P P SS+ V P P P E + Sbjct: 8 YHIFAIILMAMTVSVGCK-PKAKKPKATAPKPISSISSNSKVAPPAPPPPPPEAEKKPEK 66 Query: 227 EEEKEYIKTNLR 262 EEE + K+ ++ Sbjct: 67 EEEPKKEKSKMK 78 >U55373-1|AAC25894.1| 1829|Caenorhabditis elegans Lethal protein 418 protein. Length = 1829 Score = 27.9 bits (59), Expect = 7.9 Identities = 8/21 (38%), Positives = 11/21 (52%) Frame = +2 Query: 512 YCRWCGQGGQVFCCSDCPHVF 574 YC C Q G++ C CP + Sbjct: 258 YCEECKQDGELLLCDTCPRAY 278 >U50311-12|AAA92315.1| 169|Caenorhabditis elegans Hypothetical protein C25E10.10 protein. Length = 169 Score = 27.9 bits (59), Expect = 7.9 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = +3 Query: 627 KCVDDWKCFKCNPECLKTPACCMLGL 704 KCV+ KC KC EC + C ++ L Sbjct: 124 KCVEKNKCSKCTLECSENEKCELVEL 149 >AL021447-3|CAA16274.1| 459|Caenorhabditis elegans Hypothetical protein F19B2.7 protein. Length = 459 Score = 27.9 bits (59), Expect = 7.9 Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 1/35 (2%) Frame = +2 Query: 191 EPTDEDFQDDLNEEEKEYIKTN-LRVSNSSLKSCE 292 E +D Q+D+NEE+++ TN V+N + C+ Sbjct: 128 EHDSQDIQEDINEEQRKINNTNPTIVNNQEVYECQ 162 >AF308445-1|AAG29838.1| 1829|Caenorhabditis elegans LET-418 protein. Length = 1829 Score = 27.9 bits (59), Expect = 7.9 Identities = 8/21 (38%), Positives = 11/21 (52%) Frame = +2 Query: 512 YCRWCGQGGQVFCCSDCPHVF 574 YC C Q G++ C CP + Sbjct: 258 YCEECKQDGELLLCDTCPRAY 278 >AC006833-6|AAF60950.1| 491|Caenorhabditis elegans Hypothetical protein ZK616.1 protein. Length = 491 Score = 27.9 bits (59), Expect = 7.9 Identities = 11/40 (27%), Positives = 22/40 (55%) Frame = +2 Query: 113 IKSEGACPAPEKPASSDENVQFPPLPEPTDEDFQDDLNEE 232 +K + P PE+P +E V+ P +P+ + ++ + EE Sbjct: 378 LKEKTPEPIPEEPKKVEEEVKKPEVPKNEEPKMEEPVKEE 417 >AC006731-1|AAF60483.1| 4900|Caenorhabditis elegans Temporarily assigned gene nameprotein 80 protein. Length = 4900 Score = 27.9 bits (59), Expect = 7.9 Identities = 12/36 (33%), Positives = 17/36 (47%) Frame = +2 Query: 140 PEKPASSDENVQFPPLPEPTDEDFQDDLNEEEKEYI 247 P S V P P P + F+ DL +EE ++I Sbjct: 3493 PATQRSITHKVSLTPPPPPPSKHFEQDLTQEELDHI 3528 Database: celegans Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 12,740,198 Number of sequences in database: 27,780 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,409,070 Number of Sequences: 27780 Number of extensions: 325724 Number of successful extensions: 1646 Number of sequences better than 10.0: 31 Number of HSP's better than 10.0 without gapping: 1484 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1640 length of database: 12,740,198 effective HSP length: 80 effective length of database: 10,517,798 effective search space used: 1724918872 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -