BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0350 (739 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 62 6e-11 SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 32 0.098 SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha... 29 0.69 SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 28 1.2 SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|c... 28 1.6 SPBC21C3.05 |sap62||zinc finger protein Sap62|Schizosaccharomyce... 28 1.6 SPCC576.15c |ksg1||serine/threonine protein kinase Ksg1|Schizosa... 27 2.8 SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 25 8.5 SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|c... 25 8.5 >SPBC25H2.03 |||vacuolar protein involved in phosphoinositide metabolism|Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 62.5 bits (145), Expect = 6e-11 Identities = 32/90 (35%), Positives = 50/90 (55%), Gaps = 2/90 (2%) Frame = +1 Query: 244 EHSFVRGLCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSS--TNPNM 417 ++ VRGL KLY+KRK E+E++VK + + T +I+ +I L D + S PN Sbjct: 2 DNLLVRGLTHKLYDKRKATAYELERVVKGYLENDETEKIRAVISQLANDFVYSPARGPNA 61 Query: 418 KNGALMGLSTVAVGLGKASVDYLPELTNPI 507 G L+GL+ VA+ LG Y+ + P+ Sbjct: 62 TFGGLIGLAAVAIALGPKIDSYMESILLPV 91 Score = 52.8 bits (121), Expect = 5e-08 Identities = 21/47 (44%), Positives = 33/47 (70%) Frame = +3 Query: 513 CFSESESRVRYQAAEALFNVLKIVRGASLSQFPVIFDALAKLAADPE 653 CF++S+S++RY A E+++N+ K+ +G F +IFD L KL AD E Sbjct: 94 CFNDSDSKIRYYACESMYNIGKVAKGEVFRYFNLIFDVLCKLFADTE 140 Score = 29.9 bits (64), Expect = 0.40 Identities = 13/18 (72%), Positives = 16/18 (88%) Frame = +2 Query: 659 VKHAAELLDRLLKDIVTE 712 VK+ AELLDRL+KDIV + Sbjct: 143 VKNGAELLDRLIKDIVMQ 160 >SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2670 Score = 31.9 bits (69), Expect = 0.098 Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 1/78 (1%) Frame = +1 Query: 265 LCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALMGLS 444 L D + + R A + +++ + K + I +L VL S + + GA GLS Sbjct: 1650 LIDPVPDTRATAAKALGSLIEKLGEKKFPTLIPELFNVL----RSECSEVDRQGAAQGLS 1705 Query: 445 TVAVGLGKASV-DYLPEL 495 + GLG A + D LPE+ Sbjct: 1706 EILAGLGLARLEDVLPEI 1723 >SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase Alg10|Schizosaccharomyces pombe|chr 1|||Manual Length = 445 Score = 29.1 bits (62), Expect = 0.69 Identities = 19/47 (40%), Positives = 24/47 (51%) Frame = -3 Query: 371 INFFIWLVFFASLKSFTIFSISTPATFLFSYNLSQSPRTKLCSAVRS 231 IN+F+W FF S S+ I Y +S S R+KL SAV S Sbjct: 259 INYFLWFFFFFSFPSYII-----------KYLMSHSRRSKLLSAVFS 294 >SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1428 Score = 28.3 bits (60), Expect = 1.2 Identities = 16/58 (27%), Positives = 27/58 (46%) Frame = -3 Query: 467 LPSPTATVDRPISAPFFILGLVEDIKSWPKTLINFFIWLVFFASLKSFTIFSISTPAT 294 L +PT+ + AP +G + P+TL+N + + S + F +F PAT Sbjct: 577 LETPTSATTSKLMAPMDNIGKFSGFERPPETLLNKWRQQLESESAEKFKVFRNQLPAT 634 >SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1316 Score = 27.9 bits (59), Expect = 1.6 Identities = 17/54 (31%), Positives = 27/54 (50%) Frame = +3 Query: 453 SGAWQGISRLLTRINKSNRACFSESESRVRYQAAEALFNVLKIVRGASLSQFPV 614 SG W ISR + I K++ +E +SR+ Y + A +K + G + PV Sbjct: 1204 SGVWALISRWIPFIPKTSFPSLAEIQSRLTYVTSVATGEKVKSMPGCFYMRPPV 1257 >SPBC21C3.05 |sap62||zinc finger protein Sap62|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 27.9 bits (59), Expect = 1.6 Identities = 11/23 (47%), Positives = 15/23 (65%) Frame = -3 Query: 686 YPTIQQHASPALRISSEFSQRIE 618 YP I+ +A P RI S + QR+E Sbjct: 144 YPDIEVNAKPRYRIMSAYEQRVE 166 >SPCC576.15c |ksg1||serine/threonine protein kinase Ksg1|Schizosaccharomyces pombe|chr 3|||Manual Length = 592 Score = 27.1 bits (57), Expect = 2.8 Identities = 17/47 (36%), Positives = 22/47 (46%) Frame = -3 Query: 512 SPIGFVNSGK*STDALPSPTATVDRPISAPFFILGLVEDIKSWPKTL 372 SP+G + ST+A PSP T +R P L E+ K W L Sbjct: 410 SPLGVPSMVSASTNAAPSPVGTFNRGTLLP-CQSNLEEENKEWSSIL 455 >SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1502 Score = 25.4 bits (53), Expect = 8.5 Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 1/35 (2%) Frame = +3 Query: 597 LSQF-PVIFDALAKLAADPERR*SMLLNCWIDCSR 698 LSQF P+IF A S+ L C +DCS+ Sbjct: 964 LSQFEPLIFKCTASRICKVREIASLSLTCLLDCSK 998 >SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 386 Score = 25.4 bits (53), Expect = 8.5 Identities = 12/38 (31%), Positives = 20/38 (52%) Frame = -3 Query: 467 LPSPTATVDRPISAPFFILGLVEDIKSWPKTLINFFIW 354 L S AT+ +S + G++ I W +TL +F +W Sbjct: 112 LLSGDATILLTLSCSLIVEGVLIIINVWRETLDSFLVW 149 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,796,152 Number of Sequences: 5004 Number of extensions: 53355 Number of successful extensions: 122 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 117 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 122 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 349251756 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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