BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0350
(739 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 62 6e-11
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 32 0.098
SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha... 29 0.69
SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 28 1.2
SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|c... 28 1.6
SPBC21C3.05 |sap62||zinc finger protein Sap62|Schizosaccharomyce... 28 1.6
SPCC576.15c |ksg1||serine/threonine protein kinase Ksg1|Schizosa... 27 2.8
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 25 8.5
SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|c... 25 8.5
>SPBC25H2.03 |||vacuolar protein involved in phosphoinositide
metabolism|Schizosaccharomyces pombe|chr 2|||Manual
Length = 811
Score = 62.5 bits (145), Expect = 6e-11
Identities = 32/90 (35%), Positives = 50/90 (55%), Gaps = 2/90 (2%)
Frame = +1
Query: 244 EHSFVRGLCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSS--TNPNM 417
++ VRGL KLY+KRK E+E++VK + + T +I+ +I L D + S PN
Sbjct: 2 DNLLVRGLTHKLYDKRKATAYELERVVKGYLENDETEKIRAVISQLANDFVYSPARGPNA 61
Query: 418 KNGALMGLSTVAVGLGKASVDYLPELTNPI 507
G L+GL+ VA+ LG Y+ + P+
Sbjct: 62 TFGGLIGLAAVAIALGPKIDSYMESILLPV 91
Score = 52.8 bits (121), Expect = 5e-08
Identities = 21/47 (44%), Positives = 33/47 (70%)
Frame = +3
Query: 513 CFSESESRVRYQAAEALFNVLKIVRGASLSQFPVIFDALAKLAADPE 653
CF++S+S++RY A E+++N+ K+ +G F +IFD L KL AD E
Sbjct: 94 CFNDSDSKIRYYACESMYNIGKVAKGEVFRYFNLIFDVLCKLFADTE 140
Score = 29.9 bits (64), Expect = 0.40
Identities = 13/18 (72%), Positives = 16/18 (88%)
Frame = +2
Query: 659 VKHAAELLDRLLKDIVTE 712
VK+ AELLDRL+KDIV +
Sbjct: 143 VKNGAELLDRLIKDIVMQ 160
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 31.9 bits (69), Expect = 0.098
Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 1/78 (1%)
Frame = +1
Query: 265 LCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALMGLS 444
L D + + R A + +++ + K + I +L VL S + + GA GLS
Sbjct: 1650 LIDPVPDTRATAAKALGSLIEKLGEKKFPTLIPELFNVL----RSECSEVDRQGAAQGLS 1705
Query: 445 TVAVGLGKASV-DYLPEL 495
+ GLG A + D LPE+
Sbjct: 1706 EILAGLGLARLEDVLPEI 1723
>SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid
alpha-glucosyltransferase Alg10|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 445
Score = 29.1 bits (62), Expect = 0.69
Identities = 19/47 (40%), Positives = 24/47 (51%)
Frame = -3
Query: 371 INFFIWLVFFASLKSFTIFSISTPATFLFSYNLSQSPRTKLCSAVRS 231
IN+F+W FF S S+ I Y +S S R+KL SAV S
Sbjct: 259 INYFLWFFFFFSFPSYII-----------KYLMSHSRRSKLLSAVFS 294
>SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1428
Score = 28.3 bits (60), Expect = 1.2
Identities = 16/58 (27%), Positives = 27/58 (46%)
Frame = -3
Query: 467 LPSPTATVDRPISAPFFILGLVEDIKSWPKTLINFFIWLVFFASLKSFTIFSISTPAT 294
L +PT+ + AP +G + P+TL+N + + S + F +F PAT
Sbjct: 577 LETPTSATTSKLMAPMDNIGKFSGFERPPETLLNKWRQQLESESAEKFKVFRNQLPAT 634
>SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1316
Score = 27.9 bits (59), Expect = 1.6
Identities = 17/54 (31%), Positives = 27/54 (50%)
Frame = +3
Query: 453 SGAWQGISRLLTRINKSNRACFSESESRVRYQAAEALFNVLKIVRGASLSQFPV 614
SG W ISR + I K++ +E +SR+ Y + A +K + G + PV
Sbjct: 1204 SGVWALISRWIPFIPKTSFPSLAEIQSRLTYVTSVATGEKVKSMPGCFYMRPPV 1257
>SPBC21C3.05 |sap62||zinc finger protein Sap62|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 217
Score = 27.9 bits (59), Expect = 1.6
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -3
Query: 686 YPTIQQHASPALRISSEFSQRIE 618
YP I+ +A P RI S + QR+E
Sbjct: 144 YPDIEVNAKPRYRIMSAYEQRVE 166
>SPCC576.15c |ksg1||serine/threonine protein kinase
Ksg1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 592
Score = 27.1 bits (57), Expect = 2.8
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = -3
Query: 512 SPIGFVNSGK*STDALPSPTATVDRPISAPFFILGLVEDIKSWPKTL 372
SP+G + ST+A PSP T +R P L E+ K W L
Sbjct: 410 SPLGVPSMVSASTNAAPSPVGTFNRGTLLP-CQSNLEEENKEWSSIL 455
>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1502
Score = 25.4 bits (53), Expect = 8.5
Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +3
Query: 597 LSQF-PVIFDALAKLAADPERR*SMLLNCWIDCSR 698
LSQF P+IF A S+ L C +DCS+
Sbjct: 964 LSQFEPLIFKCTASRICKVREIASLSLTCLLDCSK 998
>SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 386
Score = 25.4 bits (53), Expect = 8.5
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = -3
Query: 467 LPSPTATVDRPISAPFFILGLVEDIKSWPKTLINFFIW 354
L S AT+ +S + G++ I W +TL +F +W
Sbjct: 112 LLSGDATILLTLSCSLIVEGVLIIINVWRETLDSFLVW 149
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,796,152
Number of Sequences: 5004
Number of extensions: 53355
Number of successful extensions: 122
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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