Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0346
(555 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si... 48 6e-06
At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co... 42 3e-04
At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si... 36 0.014
At3g19540.1 68416.m02477 expressed protein contains Pfam profile... 33 0.097
At1g49840.1 68414.m05588 expressed protein contains Pfam profile... 30 1.2
At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / hi... 29 2.8
At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly id... 29 2.8
At5g15700.1 68418.m01836 DNA-directed RNA polymerase (RPOT2) ide... 27 6.4
At4g13266.1 68417.m02071 hypothetical protein 27 6.4
At3g13340.1 68416.m01679 WD-40 repeat family protein contains Pf... 27 6.4
At1g15280.2 68414.m01829 glycine-rich protein 27 6.4
At1g15280.1 68414.m01828 glycine-rich protein 27 6.4
>At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein
similar to beta-hexosaminidase A SP:P13723 from
[Dictyostelium discoideum]
Length = 541
Score = 47.6 bits (108), Expect = 6e-06
Identities = 21/61 (34%), Positives = 32/61 (52%)
Frame = +3
Query: 72 WYLDHLKTGGDWTEFFNKDPRDMVSGLSKDINVDNIVGGEXVMWTEVVNDMNIMSRVWPR 251
WYLDHL W E +N +P ++G+ ++GGE MW E + ++ +WPR
Sbjct: 424 WYLDHLDV--PWEEVYNTEP---LNGIEDPSLQKLVIGGEVCMWGETADTSVVLQTIWPR 478
Query: 252 A 254
A
Sbjct: 479 A 479
>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
contains Pfam PF00728: Glycosyl hydrolase family 20,
catalytic domain; contains Pfam PF02838: Glycosyl
hydrolase family 20, domain 2; similar to
Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
(N-acetyl-beta- glucosaminidase)
(Beta-N-acetylhexosaminidase) (Hexosaminidase B)
(Swiss-Prot:P07686) [Homo sapiens]
Length = 535
Score = 41.9 bits (94), Expect = 3e-04
Identities = 19/61 (31%), Positives = 31/61 (50%)
Frame = +3
Query: 72 WYLDHLKTGGDWTEFFNKDPRDMVSGLSKDINVDNIVGGEXVMWTEVVNDMNIMSRVWPR 251
WYLDH+ W F+ +P ++ + ++GGE MW E ++ +I +WPR
Sbjct: 421 WYLDHIDA--PWQGFYANEPFQNITDKKQQ---SLVLGGEVCMWGEHIDASDIEQTIWPR 475
Query: 252 A 254
A
Sbjct: 476 A 476
>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
similar to beta-hexosaminidase precursor SP:P43077 from
[Candida albicans]
Length = 580
Score = 36.3 bits (80), Expect = 0.014
Identities = 17/50 (34%), Positives = 26/50 (52%)
Frame = +3
Query: 105 WTEFFNKDPRDMVSGLSKDINVDNIVGGEXVMWTEVVNDMNIMSRVWPRA 254
W +N D D GL + ++GGE +W+E + + SR+WPRA
Sbjct: 468 WQSIYNYDIAD---GLLNEEERKLVLGGEVALWSEQADSTVLDSRLWPRA 514
>At3g19540.1 68416.m02477 expressed protein contains Pfam profile
PF04788: Protein of unknown function (DUF620)
Length = 485
Score = 33.5 bits (73), Expect = 0.097
Identities = 18/51 (35%), Positives = 26/51 (50%)
Frame = -2
Query: 401 TKRLGRSQGHWVAGRVCRARSFCMCAPLSESILDMLPVIRGPTVFQLQHSS 249
+KRLG GHWV G++ RA S A + L +L + G + + SS
Sbjct: 98 SKRLGSGLGHWVKGQLSRAPSVAATAAYRRNDLRLLLGVMGAPLAPIHVSS 148
>At1g49840.1 68414.m05588 expressed protein contains Pfam profile
PF04788: Protein of unknown function (DUF620)
Length = 494
Score = 29.9 bits (64), Expect = 1.2
Identities = 21/62 (33%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
Frame = -2
Query: 401 TKRLGRSQGHWVAGRVCRARSFCMCAP-LSESILDMLPVIRGPTVFQLQHSSWPYSAHNV 225
+KRLG HWV G+ RA S P +S L +L + G + + SS S+H +
Sbjct: 105 SKRLGSGLSHWVKGQWSRAPSVTSTTPAYRKSDLRLLLGVMGAPLAPINVSS---SSHLL 161
Query: 224 HV 219
H+
Sbjct: 162 HL 163
>At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative /
histidine--tRNA ligase, putative similar to SP|P12081
Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA
ligase) (HisRS) {Homo sapiens}; contains Pfam profiles
PF00587: tRNA synthetase class II core domain (G, H, P,
S and T), PF03129: Anticodon binding domain
Length = 479
Score = 28.7 bits (61), Expect = 2.8
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +2
Query: 233 EQSMATSCAVAERLWGHESQAAYQVYSRLEEHTCRMNARGI 355
+ +A + + +LWG + A Y V R E+H R GI
Sbjct: 393 DNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAKESGI 433
>At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly
identical to auxin transport protein; BIG [Arabidopsis
thaliana] GI:21779966; contains Pfam profiles PF02207:
Putative zinc finger in N-recognin, PF00569: Zinc finger
ZZ type
Length = 5098
Score = 28.7 bits (61), Expect = 2.8
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -1
Query: 357 RMPRAFILHVCSSKREYT*YAACDSWPHS 271
R R LH+C SK +Y Y+ DSW S
Sbjct: 3023 RYARRLFLHLCGSKTQY--YSVRDSWQFS 3049
>At5g15700.1 68418.m01836 DNA-directed RNA polymerase (RPOT2)
identical to phage-type RNA polymerase rpoT2
[Arabidopsis thaliana] GI:11340683
Length = 1011
Score = 27.5 bits (58), Expect = 6.4
Identities = 21/87 (24%), Positives = 38/87 (43%), Gaps = 4/87 (4%)
Frame = -2
Query: 356 VCRARSFCMCAPLSESILDMLPVIRGPTVFQLQHSSW----PYSAHNVHVVNNFCPHDXF 189
VC + + + +P E++L +P+ + + LQH + A V++V P D +
Sbjct: 687 VCISLTEALRSPSPETVLSHIPIHQDGSCNGLQHYAALGRDTLGAEAVNLVAGEKPADVY 746
Query: 188 ATYNVVNIDVFAEAADHIPRVLVEKFR 108
+ +D+ AD P V E R
Sbjct: 747 SGIATRVLDIMRRDADRDPEVFPEALR 773
>At4g13266.1 68417.m02071 hypothetical protein
Length = 221
Score = 27.5 bits (58), Expect = 6.4
Identities = 11/27 (40%), Positives = 14/27 (51%)
Frame = +3
Query: 33 ILRASHQLIYSSGWYLDHLKTGGDWTE 113
+ R H SS W+L +L GDW E
Sbjct: 7 LTREEHPFQDSSAWFLRYLDHQGDWLE 33
>At3g13340.1 68416.m01679 WD-40 repeat family protein contains Pfam
profile: PF00400 WD domain, G-beta repeat (3 copies, 1
significant); similar to Trp-Asp repeat protein
(PIR:T40094) [Schizosaccharomyces]
Length = 447
Score = 27.5 bits (58), Expect = 6.4
Identities = 20/68 (29%), Positives = 33/68 (48%), Gaps = 4/68 (5%)
Frame = -2
Query: 269 FQLQHSSWPYSAHNVHVVNNF-CPHDXFAT---YNVVNIDVFAEAADHIPRVLVEKFRPI 102
FQL++ W S H+V++++NF H T + V+N+ ++ P L+E F
Sbjct: 132 FQLRNLVWATSKHDVYLMSNFLVTHYSSLTSGKHEVLNVRGHVAPSEKHPGSLLEGFTQT 191
Query: 101 ASSFQMVK 78
S VK
Sbjct: 192 QVSTLAVK 199
>At1g15280.2 68414.m01829 glycine-rich protein
Length = 585
Score = 27.5 bits (58), Expect = 6.4
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -2
Query: 296 LPVIRGPTVFQLQHSSWPYSAHNVH 222
+PV+ GP +S PY+AH H
Sbjct: 502 MPVLAGPGALGASYSPPPYAAHQAH 526
>At1g15280.1 68414.m01828 glycine-rich protein
Length = 584
Score = 27.5 bits (58), Expect = 6.4
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -2
Query: 296 LPVIRGPTVFQLQHSSWPYSAHNVH 222
+PV+ GP +S PY+AH H
Sbjct: 501 MPVLAGPGALGASYSPPPYAAHQAH 525
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,505,955
Number of Sequences: 28952
Number of extensions: 218204
Number of successful extensions: 543
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 543
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1053014392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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