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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0343
         (670 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po...   133   2e-32
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po...   126   2e-30
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ...    29   0.80 
SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosac...    28   1.1  
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy...    27   1.8  
SPACUNK4.14 |mdb1||BRCT domain protein|Schizosaccharomyces pombe...    27   2.4  
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ...    27   3.2  
SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|c...    26   4.3  
SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|...    26   5.6  
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc...    26   5.6  
SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|ch...    26   5.6  
SPAC869.02c |||nitric oxide dioxygenase |Schizosaccharomyces pom...    25   7.5  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    25   7.5  
SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizos...    25   9.9  
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr...    25   9.9  
SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|...    25   9.9  
SPBC18H10.03 |tif35||translation initiation factor eIF3g|Schizos...    25   9.9  

>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  133 bits (322), Expect = 2e-32
 Identities = 62/99 (62%), Positives = 79/99 (79%)
 Frame = +2

Query: 251 QDVIGARRASWRIISSIEQKEETKGXEDKLNMIRAYRSQVEXELRDICSDILGVLDKYLI 430
           +++IGARRASWRIISSIEQKEE++G   +  +I+ YR ++E EL DIC D+L VL+K+LI
Sbjct: 51  KNIIGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLI 110

Query: 431 PSSQTGESKVFYYKMKGDYHRYLAEFRPATTARGCRELA 547
           P++ TGESKVFYYKMKGDY+RYLAEF   T    C+E A
Sbjct: 111 PAATTGESKVFYYKMKGDYYRYLAEF---TVGEVCKEAA 146



 Score =  119 bits (286), Expect = 4e-28
 Identities = 78/191 (40%), Positives = 101/191 (52%), Gaps = 1/191 (0%)
 Frame = +3

Query: 99  SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKTXXXXXXX 278
           + RE++VY AKLAEQAERY+EMVE MK VA  N   ++L+VEERNLLSVAYK        
Sbjct: 3   NSRENSVYLAKLAEQAERYEEMVENMKKVACSN---DKLSVEERNLLSVAYKNIIGARRA 59

Query: 279 XXXXXXXXNRRKKQKAXKTN*T*SEXXXXXXXXXXXXXXXXXXXXXXXXXXPAHRPENQK 458
                    ++++ +         +                          PA      K
Sbjct: 60  SWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESK 119

Query: 459 FFITK*RATTTGTWPS-SDRQRPQEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS 635
            F  K +          +  +  +EAA++SL AYKAASDIA+ ELPPT P+RLGLALNFS
Sbjct: 120 VFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFS 179

Query: 636 VFYYGILNSPD 668
           VFYY IL+SP+
Sbjct: 180 VFYYEILDSPE 190


>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  126 bits (305), Expect = 2e-30
 Identities = 57/86 (66%), Positives = 73/86 (84%)
 Frame = +2

Query: 251 QDVIGARRASWRIISSIEQKEETKGXEDKLNMIRAYRSQVEXELRDICSDILGVLDKYLI 430
           ++VIGARRASWRI+SSIEQKEE+KG   ++ +I+ YR ++E EL  IC DIL VL+K+LI
Sbjct: 52  KNVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLI 111

Query: 431 PSSQTGESKVFYYKMKGDYHRYLAEF 508
           P++ + ESKVFYYKMKGDY+RYLAEF
Sbjct: 112 PNAASAESKVFYYKMKGDYYRYLAEF 137



 Score =  124 bits (298), Expect = 2e-29
 Identities = 81/191 (42%), Positives = 97/191 (50%), Gaps = 1/191 (0%)
 Frame = +3

Query: 99  SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKTXXXXXXX 278
           + RED VY AKLAEQAERY+ MVE MK+VAS   +D ELTVEERNLLSVAYK        
Sbjct: 4   TSREDAVYLAKLAEQAERYEGMVENMKSVAS---TDQELTVEERNLLSVAYKNVIGARRA 60

Query: 279 XXXXXXXXNRRKKQKAXKTN*T*SEXXXXXXXXXXXXXXXXXXXXXXXXXXPAHRPENQK 458
                    ++++ K         +                          P       K
Sbjct: 61  SWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESK 120

Query: 459 FFITK*RATTTGTWPS-SDRQRPQEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS 635
            F  K +          +  ++ Q +A+ SL  YKAAS+IA  EL PTHPIRLGLALNFS
Sbjct: 121 VFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFS 180

Query: 636 VFYYGILNSPD 668
           VFYY ILNSPD
Sbjct: 181 VFYYEILNSPD 191


>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
            zf-fungal binuclear cluster type |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 977

 Score = 28.7 bits (61), Expect = 0.80
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = +3

Query: 492  GTWPSSDRQRPQEAAENSLVAY 557
            G WPS D +  QE+  +SLVAY
Sbjct: 943  GLWPSDDLRNYQESTIDSLVAY 964


>SPAC23C4.02 |crn1||actin binding protein, coronin
           Crn1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 601

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 12/36 (33%), Positives = 18/36 (50%)
 Frame = +2

Query: 446 GESKVFYYKMKGDYHRYLAEFRPATTARGCRELAGR 553
           G+  + YY+ + D   YL+EF+     RG   L  R
Sbjct: 289 GDGNIRYYEYENDVFHYLSEFKSVDPQRGIAFLPKR 324


>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 541

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 12/32 (37%), Positives = 14/32 (43%)
 Frame = +2

Query: 455 KVFYYKMKGDYHRYLAEFRPATTARGCRELAG 550
           K F    K DY +Y+  FR    A  CR   G
Sbjct: 171 KEFLAHSKDDYAQYMIPFRDINVATSCRTFCG 202


>SPACUNK4.14 |mdb1||BRCT domain protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 520

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -2

Query: 453 DSPVCELGMRYLSRTPRMSEQMSRSSFSTW 364
           DSP   +G R +S+  R  +   RSS S+W
Sbjct: 481 DSPTKVIGKRNVSKASRTGQGRKRSSRSSW 510


>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
           Cct4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 527

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 10/29 (34%), Positives = 19/29 (65%)
 Frame = +3

Query: 510 DRQRPQEAAENSLVAYKAASDIAMTELPP 596
           DR++PQE   ++++A ++ +D   T L P
Sbjct: 13  DREKPQEVRLSNIMAARSVADAIRTSLGP 41


>SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 599

 Score = 26.2 bits (55), Expect = 4.3
 Identities = 10/37 (27%), Positives = 22/37 (59%)
 Frame = -2

Query: 408 PRMSEQMSRSSFSTWLRYALIMFNLSSXPFVSSFCSM 298
           P ++E  + +S S+W+  A ++ + +  P V  FC++
Sbjct: 121 PTIAEDFNDTSSSSWIGSAYVLASNAVLPAVGVFCNI 157


>SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 736

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 2/43 (4%)
 Frame = -2

Query: 216 SAHCRTRCVMLHSSSPPPFRHSAQPAQLTLLYT--HYLPFPTL 94
           S+H   + +  H    P F  S    QLTLL +   YLP P +
Sbjct: 486 SSHREKKSIYQHKEYMPDFLSSRDEFQLTLLESTWQYLPDPNV 528


>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
           Mis17|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 441

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 13/48 (27%), Positives = 24/48 (50%)
 Frame = +3

Query: 87  SSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEER 230
           SST S    N    ++  Q+E Y ++ +   + +  ++SD E  +E R
Sbjct: 45  SSTRSSPASNQQNLEIVSQSEAYHKISDLDDSTSIFSISDQENILERR 92


>SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 867

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 13/36 (36%), Positives = 20/36 (55%)
 Frame = +3

Query: 504 SSDRQRPQEAAENSLVAYKAASDIAMTELPPTHPIR 611
           +S   RP ++  N   +Y+ AS    T+ PP HP+R
Sbjct: 390 NSPSLRPLQSLNNVQNSYRVAS----TQAPPPHPLR 421


>SPAC869.02c |||nitric oxide dioxygenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 12/31 (38%), Positives = 16/31 (51%)
 Frame = +2

Query: 425 LIPSSQTGESKVFYYKMKGDYHRYLAEFRPA 517
           ++ SS    +K FY KM G+Y   L  F  A
Sbjct: 45  ILESSGVNLTKAFYQKMLGNYPEVLPYFNKA 75


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 10/32 (31%), Positives = 19/32 (59%)
 Frame = -2

Query: 264 PITSCRRPTAGSSPPLSAHCRTRCVMLHSSSP 169
           P+T    PT+ S+P  + +C T   + ++S+P
Sbjct: 408 PVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTP 439


>SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate
           synthase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 584

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -2

Query: 120 THYLPFPTLWMTNDFTLTKS 61
           T+YL FP  +  ND+TL+ +
Sbjct: 455 TYYLEFPGRFSVNDYTLSSN 474


>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 661

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 20/55 (36%), Positives = 26/55 (47%), Gaps = 12/55 (21%)
 Frame = +3

Query: 117 VYKAKLAEQAE----RYDEMVEAMKNVASR--------NVSDNELTVEERNLLSV 245
           +YK KL    E    R DE+V  MKN+  +        N SD  LT+ E  L S+
Sbjct: 202 IYKEKLRFSLESRKSRVDEIVNEMKNMCGKNPTSELPTNYSDKTLTLWENELSSM 256


>SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 809

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 9/31 (29%), Positives = 18/31 (58%)
 Frame = +3

Query: 108 EDNVYKAKLAEQAERYDEMVEAMKNVASRNV 200
           ED++++  + +Q   Y E +E  K   ++NV
Sbjct: 713 EDSMHEIAIKKQHSTYSEALEVAKKAGTKNV 743


>SPBC18H10.03 |tif35||translation initiation factor
           eIF3g|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 282

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +2

Query: 371 EXELRDICSDILGVLDKYLIPSSQTGESKVFYY 469
           E ELRD+     G+   YL    +TG +K F +
Sbjct: 215 EEELRDLFRRFGGIQRVYLAKDKETGRAKGFAF 247


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,372,563
Number of Sequences: 5004
Number of extensions: 44146
Number of successful extensions: 163
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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