BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0343 (670 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 133 2e-32 SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 126 2e-30 SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 29 0.80 SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosac... 28 1.1 SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 27 1.8 SPACUNK4.14 |mdb1||BRCT domain protein|Schizosaccharomyces pombe... 27 2.4 SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 27 3.2 SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|c... 26 4.3 SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|... 26 5.6 SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 26 5.6 SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|ch... 26 5.6 SPAC869.02c |||nitric oxide dioxygenase |Schizosaccharomyces pom... 25 7.5 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 7.5 SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizos... 25 9.9 SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 25 9.9 SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|... 25 9.9 SPBC18H10.03 |tif35||translation initiation factor eIF3g|Schizos... 25 9.9 >SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 133 bits (322), Expect = 2e-32 Identities = 62/99 (62%), Positives = 79/99 (79%) Frame = +2 Query: 251 QDVIGARRASWRIISSIEQKEETKGXEDKLNMIRAYRSQVEXELRDICSDILGVLDKYLI 430 +++IGARRASWRIISSIEQKEE++G + +I+ YR ++E EL DIC D+L VL+K+LI Sbjct: 51 KNIIGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLI 110 Query: 431 PSSQTGESKVFYYKMKGDYHRYLAEFRPATTARGCRELA 547 P++ TGESKVFYYKMKGDY+RYLAEF T C+E A Sbjct: 111 PAATTGESKVFYYKMKGDYYRYLAEF---TVGEVCKEAA 146 Score = 119 bits (286), Expect = 4e-28 Identities = 78/191 (40%), Positives = 101/191 (52%), Gaps = 1/191 (0%) Frame = +3 Query: 99 SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKTXXXXXXX 278 + RE++VY AKLAEQAERY+EMVE MK VA N ++L+VEERNLLSVAYK Sbjct: 3 NSRENSVYLAKLAEQAERYEEMVENMKKVACSN---DKLSVEERNLLSVAYKNIIGARRA 59 Query: 279 XXXXXXXXNRRKKQKAXKTN*T*SEXXXXXXXXXXXXXXXXXXXXXXXXXXPAHRPENQK 458 ++++ + + PA K Sbjct: 60 SWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESK 119 Query: 459 FFITK*RATTTGTWPS-SDRQRPQEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS 635 F K + + + +EAA++SL AYKAASDIA+ ELPPT P+RLGLALNFS Sbjct: 120 VFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFS 179 Query: 636 VFYYGILNSPD 668 VFYY IL+SP+ Sbjct: 180 VFYYEILDSPE 190 >SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 126 bits (305), Expect = 2e-30 Identities = 57/86 (66%), Positives = 73/86 (84%) Frame = +2 Query: 251 QDVIGARRASWRIISSIEQKEETKGXEDKLNMIRAYRSQVEXELRDICSDILGVLDKYLI 430 ++VIGARRASWRI+SSIEQKEE+KG ++ +I+ YR ++E EL IC DIL VL+K+LI Sbjct: 52 KNVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLI 111 Query: 431 PSSQTGESKVFYYKMKGDYHRYLAEF 508 P++ + ESKVFYYKMKGDY+RYLAEF Sbjct: 112 PNAASAESKVFYYKMKGDYYRYLAEF 137 Score = 124 bits (298), Expect = 2e-29 Identities = 81/191 (42%), Positives = 97/191 (50%), Gaps = 1/191 (0%) Frame = +3 Query: 99 SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKTXXXXXXX 278 + RED VY AKLAEQAERY+ MVE MK+VAS +D ELTVEERNLLSVAYK Sbjct: 4 TSREDAVYLAKLAEQAERYEGMVENMKSVAS---TDQELTVEERNLLSVAYKNVIGARRA 60 Query: 279 XXXXXXXXNRRKKQKAXKTN*T*SEXXXXXXXXXXXXXXXXXXXXXXXXXXPAHRPENQK 458 ++++ K + P K Sbjct: 61 SWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESK 120 Query: 459 FFITK*RATTTGTWPS-SDRQRPQEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS 635 F K + + ++ Q +A+ SL YKAAS+IA EL PTHPIRLGLALNFS Sbjct: 121 VFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFS 180 Query: 636 VFYYGILNSPD 668 VFYY ILNSPD Sbjct: 181 VFYYEILNSPD 191 >SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 977 Score = 28.7 bits (61), Expect = 0.80 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = +3 Query: 492 GTWPSSDRQRPQEAAENSLVAY 557 G WPS D + QE+ +SLVAY Sbjct: 943 GLWPSDDLRNYQESTIDSLVAY 964 >SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/36 (33%), Positives = 18/36 (50%) Frame = +2 Query: 446 GESKVFYYKMKGDYHRYLAEFRPATTARGCRELAGR 553 G+ + YY+ + D YL+EF+ RG L R Sbjct: 289 GDGNIRYYEYENDVFHYLSEFKSVDPQRGIAFLPKR 324 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 27.5 bits (58), Expect = 1.8 Identities = 12/32 (37%), Positives = 14/32 (43%) Frame = +2 Query: 455 KVFYYKMKGDYHRYLAEFRPATTARGCRELAG 550 K F K DY +Y+ FR A CR G Sbjct: 171 KEFLAHSKDDYAQYMIPFRDINVATSCRTFCG 202 >SPACUNK4.14 |mdb1||BRCT domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 520 Score = 27.1 bits (57), Expect = 2.4 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = -2 Query: 453 DSPVCELGMRYLSRTPRMSEQMSRSSFSTW 364 DSP +G R +S+ R + RSS S+W Sbjct: 481 DSPTKVIGKRNVSKASRTGQGRKRSSRSSW 510 >SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit Cct4|Schizosaccharomyces pombe|chr 2|||Manual Length = 527 Score = 26.6 bits (56), Expect = 3.2 Identities = 10/29 (34%), Positives = 19/29 (65%) Frame = +3 Query: 510 DRQRPQEAAENSLVAYKAASDIAMTELPP 596 DR++PQE ++++A ++ +D T L P Sbjct: 13 DREKPQEVRLSNIMAARSVADAIRTSLGP 41 >SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 599 Score = 26.2 bits (55), Expect = 4.3 Identities = 10/37 (27%), Positives = 22/37 (59%) Frame = -2 Query: 408 PRMSEQMSRSSFSTWLRYALIMFNLSSXPFVSSFCSM 298 P ++E + +S S+W+ A ++ + + P V FC++ Sbjct: 121 PTIAEDFNDTSSSSWIGSAYVLASNAVLPAVGVFCNI 157 >SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 736 Score = 25.8 bits (54), Expect = 5.6 Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 2/43 (4%) Frame = -2 Query: 216 SAHCRTRCVMLHSSSPPPFRHSAQPAQLTLLYT--HYLPFPTL 94 S+H + + H P F S QLTLL + YLP P + Sbjct: 486 SSHREKKSIYQHKEYMPDFLSSRDEFQLTLLESTWQYLPDPNV 528 >SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosaccharomyces pombe|chr 2|||Manual Length = 441 Score = 25.8 bits (54), Expect = 5.6 Identities = 13/48 (27%), Positives = 24/48 (50%) Frame = +3 Query: 87 SSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEER 230 SST S N ++ Q+E Y ++ + + + ++SD E +E R Sbjct: 45 SSTRSSPASNQQNLEIVSQSEAYHKISDLDDSTSIFSISDQENILERR 92 >SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|chr 3|||Manual Length = 867 Score = 25.8 bits (54), Expect = 5.6 Identities = 13/36 (36%), Positives = 20/36 (55%) Frame = +3 Query: 504 SSDRQRPQEAAENSLVAYKAASDIAMTELPPTHPIR 611 +S RP ++ N +Y+ AS T+ PP HP+R Sbjct: 390 NSPSLRPLQSLNNVQNSYRVAS----TQAPPPHPLR 421 >SPAC869.02c |||nitric oxide dioxygenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 25.4 bits (53), Expect = 7.5 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = +2 Query: 425 LIPSSQTGESKVFYYKMKGDYHRYLAEFRPA 517 ++ SS +K FY KM G+Y L F A Sbjct: 45 ILESSGVNLTKAFYQKMLGNYPEVLPYFNKA 75 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 25.4 bits (53), Expect = 7.5 Identities = 10/32 (31%), Positives = 19/32 (59%) Frame = -2 Query: 264 PITSCRRPTAGSSPPLSAHCRTRCVMLHSSSP 169 P+T PT+ S+P + +C T + ++S+P Sbjct: 408 PVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTP 439 >SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 584 Score = 25.0 bits (52), Expect = 9.9 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = -2 Query: 120 THYLPFPTLWMTNDFTLTKS 61 T+YL FP + ND+TL+ + Sbjct: 455 TYYLEFPGRFSVNDYTLSSN 474 >SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 661 Score = 25.0 bits (52), Expect = 9.9 Identities = 20/55 (36%), Positives = 26/55 (47%), Gaps = 12/55 (21%) Frame = +3 Query: 117 VYKAKLAEQAE----RYDEMVEAMKNVASR--------NVSDNELTVEERNLLSV 245 +YK KL E R DE+V MKN+ + N SD LT+ E L S+ Sbjct: 202 IYKEKLRFSLESRKSRVDEIVNEMKNMCGKNPTSELPTNYSDKTLTLWENELSSM 256 >SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|||Manual Length = 809 Score = 25.0 bits (52), Expect = 9.9 Identities = 9/31 (29%), Positives = 18/31 (58%) Frame = +3 Query: 108 EDNVYKAKLAEQAERYDEMVEAMKNVASRNV 200 ED++++ + +Q Y E +E K ++NV Sbjct: 713 EDSMHEIAIKKQHSTYSEALEVAKKAGTKNV 743 >SPBC18H10.03 |tif35||translation initiation factor eIF3g|Schizosaccharomyces pombe|chr 2|||Manual Length = 282 Score = 25.0 bits (52), Expect = 9.9 Identities = 12/33 (36%), Positives = 17/33 (51%) Frame = +2 Query: 371 EXELRDICSDILGVLDKYLIPSSQTGESKVFYY 469 E ELRD+ G+ YL +TG +K F + Sbjct: 215 EEELRDLFRRFGGIQRVYLAKDKETGRAKGFAF 247 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,372,563 Number of Sequences: 5004 Number of extensions: 44146 Number of successful extensions: 163 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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