SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0333
         (727 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC16C4.03 |pin1||peptidyl-prolyl cis-trans isomerase Pin1|Schi...   106   4e-24
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy...    40   5e-04
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc...    35   0.014
SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomy...    32   0.073
SPAP14E8.03 |bos1||SNARE Bos1|Schizosaccharomyces pombe|chr 1|||...    29   0.68 
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe...    29   0.89 
SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi...    28   1.2  
SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom...    28   1.6  
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch...    27   2.1  
SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pom...    27   2.7  
SPAC1B3.08 |||COP9 signalosome complex subunit 12 |Schizosacchar...    27   3.6  
SPAC8E11.08c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual       27   3.6  
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S...    27   3.6  
SPBC83.03c |tas3||RITS complex subunit 3 |Schizosaccharomyces po...    26   4.8  
SPBC4B4.03 |rsc1||RSC complex subunit Rsc1 |Schizosaccharomyces ...    26   6.3  
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb...    26   6.3  
SPBC1734.16c |pst3||SIN3 family co-repressor|Schizosaccharomyces...    26   6.3  

>SPCC16C4.03 |pin1||peptidyl-prolyl cis-trans isomerase
           Pin1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 175

 Score =  106 bits (254), Expect = 4e-24
 Identities = 48/84 (57%), Positives = 60/84 (71%)
 Frame = +2

Query: 257 HLLVKHSGSRRPSSWREEHITRTKEEALDILQEYRRKIIDREAKFEELASTYSDCSSAKR 436
           HLLVKH  SRRPSSW+EEHITR+KEEA  + + Y + +        +LA   SDCSSA+R
Sbjct: 71  HLLVKHRESRRPSSWKEEHITRSKEEARKLAEHYEQLLKSGSVSMHDLAMKESDCSSARR 130

Query: 437 DGDLGRFKKGQMQKPFEDVAFSLK 508
            G+LG F + +MQKPFED AF+LK
Sbjct: 131 GGELGEFGRDEMQKPFEDAAFALK 154



 Score = 32.7 bits (71), Expect = 0.055
 Identities = 13/31 (41%), Positives = 17/31 (54%)
 Frame = +3

Query: 90  LPEGWEARKSRSTGMTYYLNKHTKKSQWEKP 182
           LP+ W  + SRS    Y+ N  T +S WE P
Sbjct: 6   LPKPWIVKISRSRNRPYFFNTETHESLWEPP 36


>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 786

 Score = 39.5 bits (88), Expect = 5e-04
 Identities = 19/57 (33%), Positives = 33/57 (57%), Gaps = 2/57 (3%)
 Frame = +3

Query: 18  RKRKNLLAFPAQRTNDMASTQEEI--LPEGWEARKSRSTGMTYYLNKHTKKSQWEKP 182
           R+  N  A      + M+S +++   LP GWE R++ S G TYY++ +T+ + W +P
Sbjct: 212 RQTNNTSALSNSNAHIMSSFEDQYGRLPPGWE-RRADSLGRTYYVDHNTRTTTWTRP 267


>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 767

 Score = 34.7 bits (76), Expect = 0.014
 Identities = 14/36 (38%), Positives = 23/36 (63%)
 Frame = +3

Query: 90  LPEGWEARKSRSTGMTYYLNKHTKKSQWEKPGGPAS 197
           LP GWE R + +T   Y+++ +TK + W+ P  P+S
Sbjct: 347 LPSGWEMRLT-NTARVYFVDHNTKTTTWDDPRLPSS 381


>SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 411

 Score = 32.3 bits (70), Expect = 0.073
 Identities = 14/31 (45%), Positives = 19/31 (61%)
 Frame = +3

Query: 90  LPEGWEARKSRSTGMTYYLNKHTKKSQWEKP 182
           LP GW   K+ S G+ YY N   KKS +++P
Sbjct: 5   LPPGWTEHKAPS-GIPYYWNAELKKSTYQRP 34


>SPAP14E8.03 |bos1||SNARE Bos1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 235

 Score = 29.1 bits (62), Expect = 0.68
 Identities = 15/49 (30%), Positives = 27/49 (55%)
 Frame = +2

Query: 302 REEHITRTKEEALDILQEYRRKIIDREAKFEELASTYSDCSSAKRDGDL 448
           + E +   K++A   +QE+R+K +    KF+EL +   D + AK   +L
Sbjct: 62  QRELVPAKKKKATIRIQEFRQKHVQLLEKFDELKAHVRDIAQAKNRKEL 110


>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 273

 Score = 28.7 bits (61), Expect = 0.89
 Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
 Frame = +3

Query: 84  EILPEGWEARKSRSTGMTYYLNKHTK--KSQWEKP 182
           E LP GW A+     G  +Y+N+  +  + QWE P
Sbjct: 8   EGLPSGWVAQWDAEYGTYFYVNESAQNPQPQWEPP 42


>SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein
           Usp104|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 695

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = +3

Query: 102 WEARKSRSTGMTYYLNKHTKKSQWEKP 182
           W   K+  + + YY N  T+KS WEKP
Sbjct: 36  WHEVKTEDSRV-YYYNSVTRKSVWEKP 61


>SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 204

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 15/41 (36%), Positives = 20/41 (48%), Gaps = 2/41 (4%)
 Frame = +3

Query: 66  MASTQEEILPEGWEARKSRSTGMTYYLNKHTKKS--QWEKP 182
           MA    E LP GW A+        +Y+N+   K+  QWE P
Sbjct: 1   MAYQTREGLPNGWVAQWDERYKCYFYVNESDPKAKPQWECP 41


>SPCC162.08c |nup211||nuclear pore complex associated
            protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1837

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 24/75 (32%), Positives = 38/75 (50%), Gaps = 2/75 (2%)
 Frame = +2

Query: 296  SWREEHITRTKEEALDILQEYRRKIIDREAKFEELASTYSDCSSAKRDGDLGRFKKG--Q 469
            S REE IT  + E LD+ +       ++E+  +ELA    D +  ++D  L  FKK   +
Sbjct: 999  STREEKITSLRSELLDLNKRVEVLKEEKESSSKELAKQLED-AVREKDSALS-FKKDYEK 1056

Query: 470  MQKPFEDVAFSLK*D 514
            ++   + V  SLK D
Sbjct: 1057 IRSDADRVITSLKED 1071


>SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 781

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
 Frame = -3

Query: 662 T*LYLRYCGYIS-LILWFYTFLSSINCE 582
           T L+  +C Y+S LILW Y ++S   CE
Sbjct: 684 TGLHRPWCLYVSTLILWAYGYVSDGRCE 711


>SPAC1B3.08 |||COP9 signalosome complex subunit 12
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 423

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = +2

Query: 383 AKFEELASTYSDCSSAKRDGDLGRFKK 463
           +KF  LAS Y   + A + G+LG F K
Sbjct: 295 SKFPNLASVYIPLTRALKSGNLGEFGK 321


>SPAC8E11.08c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 100

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 8/19 (42%), Positives = 13/19 (68%)
 Frame = -1

Query: 535 CELVGSIVLFQGKCDVFKW 479
           C    ++V F GKCD+F++
Sbjct: 81  CSFPNNLVFFMGKCDLFRF 99


>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 971

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 11/31 (35%), Positives = 19/31 (61%)
 Frame = -3

Query: 707 KEDSLNLCSRKKKIYT*LYLRYCGYISLILW 615
           K+DS   CS+K+ +++ LY     Y+S + W
Sbjct: 263 KKDSNVKCSKKQILFSLLYFSSEVYLSFVFW 293


>SPBC83.03c |tas3||RITS complex subunit 3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 549

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 12/29 (41%), Positives = 19/29 (65%)
 Frame = -2

Query: 576 SLGSSKNDMNARVSVNWLAQLSYFKENAT 490
           SL + KND+     +NWL  LS+F+ N++
Sbjct: 430 SLTTDKNDLY----INWLKSLSFFQTNSS 454


>SPBC4B4.03 |rsc1||RSC complex subunit Rsc1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 803

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
 Frame = +2

Query: 308 EHITRTKEEALDILQEYRRKI-IDREAKFEELASTYSD 418
           E++   K +   ++ E  R   + REAKFE L ++ SD
Sbjct: 761 EYVLYKKSKGSQVITEKARSNELSREAKFENLVASLSD 798


>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 522

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 14/50 (28%), Positives = 23/50 (46%)
 Frame = +3

Query: 228 GGIPKEVRCATCL*NTVEAADHPHGVKSILHVLRRKLLIYYKSIAVKSLT 377
           G  P+ V+C TCL +  +      G+K I  +  + +     + A K LT
Sbjct: 185 GHSPELVQCITCLPDVADHLTSHSGIKHITFIGSQPIAKLVAASAAKQLT 234


>SPBC1734.16c |pst3||SIN3 family co-repressor|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1154

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 11/22 (50%), Positives = 16/22 (72%), Gaps = 1/22 (4%)
 Frame = -3

Query: 449 PNPHHVL-LMNNQNMYLPALQI 387
           P P HV  +M+++N YL ALQ+
Sbjct: 532 PKPSHVKNIMHHENQYLQALQL 553


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,948,836
Number of Sequences: 5004
Number of extensions: 57841
Number of successful extensions: 190
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -