BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0321 (632 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|c... 29 0.74 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 27 1.7 SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 27 2.3 SPCC1183.06 |ung1||uracil DNA N-glycosylase Ung1|Schizosaccharom... 27 2.3 SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 27 2.3 SPAC589.07c |||WD repeat protein Atg18|Schizosaccharomyces pombe... 27 2.3 SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pomb... 27 3.0 SPCC417.05c |chr2|cfh2|chitin synthase regulatory factor |Schizo... 26 3.9 SPAC17A5.09c |||protein phosphatase regulatory subunit Glc9|Schi... 26 3.9 SPCC162.11c |||uridine kinase |Schizosaccharomyces pombe|chr 3||... 25 6.9 SPBC216.06c |swi1||replication fork protection complex subunit S... 25 6.9 SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 9.1 SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 25 9.1 SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|S... 25 9.1 SPBC31F10.10c |||zf-MYND type zinc finger protein|Schizosaccharo... 25 9.1 >SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 392 Score = 28.7 bits (61), Expect = 0.74 Identities = 21/75 (28%), Positives = 30/75 (40%), Gaps = 9/75 (12%) Frame = +2 Query: 239 RVLWNSPGTSAATNPIAMATVFKNTVFT--------NVAETPEGGVWWEGMGPA-PERLV 391 +V W PG + T + M V K+T F N A P + E A P+ Sbjct: 11 KVQWGRPGNNLKTGIVGMPNVGKSTFFRAITKSVLGNPANYPYATIDPEEAKVAVPDERF 70 Query: 392 DWKGQPWDPSKKTPA 436 DW + + P + PA Sbjct: 71 DWLCEAYKPKSRVPA 85 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 27.5 bits (58), Expect = 1.7 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = -2 Query: 466 RGAESGIWMCSRSFLAGVPGLTFPVDEALRSWS 368 R ESG S++ + GV L F D+ LR W+ Sbjct: 175 RFLESGFLRLSKNLVPGVISLLFSRDDELRRWA 207 >SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 344 Score = 27.1 bits (57), Expect = 2.3 Identities = 11/24 (45%), Positives = 12/24 (50%) Frame = +3 Query: 348 GCGGKAWDQLRSASSTGKVNPGTP 419 G G W L+S TGK N G P Sbjct: 216 GYGATVWSPLKSGILTGKYNDGIP 239 >SPCC1183.06 |ung1||uracil DNA N-glycosylase Ung1|Schizosaccharomyces pombe|chr 3|||Manual Length = 322 Score = 27.1 bits (57), Expect = 2.3 Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 1/45 (2%) Frame = -2 Query: 400 FPVDEALRSWSHAFPPHPT-LRCFRHVSEHGVFEHRRHCYRIRSG 269 FP E + SWSH P H T + H + + C+ +R G Sbjct: 116 FPPKEDIYSWSHHTPLHKTKVILLGQDPYHNIGQAHGLCFSVRPG 160 >SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1338 Score = 27.1 bits (57), Expect = 2.3 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +3 Query: 384 ASSTGKVNPGTPARKLRLHIQIPDSAPRQSSV 479 AS+ G V P+ R+H +P+SAP S+ Sbjct: 555 ASNLGDVGLRLPSPSPRIHTIVPNSAPEHPSI 586 >SPAC589.07c |||WD repeat protein Atg18|Schizosaccharomyces pombe|chr 1|||Manual Length = 373 Score = 27.1 bits (57), Expect = 2.3 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +3 Query: 159 KVECVGDDIAWMKFDKDGVLRAINSENG 242 K+E D +A + F+ DG + A S+NG Sbjct: 183 KIEAHKDSLACLAFNSDGTMLATASDNG 210 >SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 26.6 bits (56), Expect = 3.0 Identities = 10/37 (27%), Positives = 18/37 (48%) Frame = +2 Query: 305 KNTVFTNVAETPEGGVWWEGMGPAPERLVDWKGQPWD 415 K+ +F +A + WW+ + P+PE+ W D Sbjct: 400 KDEIFKQIAFSVVEKAWWKMLLPSPEQWNSWPSSSRD 436 >SPCC417.05c |chr2|cfh2|chitin synthase regulatory factor |Schizosaccharomyces pombe|chr 3|||Manual Length = 512 Score = 26.2 bits (55), Expect = 3.9 Identities = 17/66 (25%), Positives = 29/66 (43%), Gaps = 3/66 (4%) Frame = -2 Query: 490 VYHGTLLCRGAESGIWMCSRSFLAGVPGLTFPVDEALRSWSH--AFPPHPTLRCF-RHVS 320 V++ L + +W S+ +L GV + +P E W+ A+ H F + V Sbjct: 404 VWYRRLARKRNPEAMWKLSQFYLNGVDDVIYPNPELANEWAKAAAYKNHHLASTFVQDVG 463 Query: 319 EHGVFE 302 + VFE Sbjct: 464 KEDVFE 469 >SPAC17A5.09c |||protein phosphatase regulatory subunit Glc9|Schizosaccharomyces pombe|chr 1|||Manual Length = 310 Score = 26.2 bits (55), Expect = 3.9 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = -1 Query: 581 RDSTTSGTPAGRLPPSRMADIGTPSSSPTHRLSWD 477 + + S TP+ + S + GTPS RL WD Sbjct: 27 QQNMVSSTPSIDMNESDCSGTGTPSEERIRRLRWD 61 >SPCC162.11c |||uridine kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 454 Score = 25.4 bits (53), Expect = 6.9 Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 1/61 (1%) Frame = +3 Query: 66 PQGKKRYIAAAFPSACGKTNLA-MMTPTLPGYKVECVGDDIAWMKFDKDGVLRAINSENG 242 P K R+I A PS GKT++A ++ L V + D + + + RA N++ Sbjct: 17 PWRKVRFIGIAGPSGSGKTSVAQLIVKALNLPHVVILSLDSFYKSLNAEQKKRAFNNDYD 76 Query: 243 F 245 F Sbjct: 77 F 77 >SPBC216.06c |swi1||replication fork protection complex subunit Swi1|Schizosaccharomyces pombe|chr 2|||Manual Length = 971 Score = 25.4 bits (53), Expect = 6.9 Identities = 14/38 (36%), Positives = 19/38 (50%) Frame = -1 Query: 299 PSPLLSDS*RLTYLVNSKEPVFRVDGPQYAVLVELHPG 186 P P L+D+ YL K P+F + G + L E PG Sbjct: 664 PFPKLTDT--FYYLEYGKSPLFSIHGSRKGPLYETVPG 699 >SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 395 Score = 25.0 bits (52), Expect = 9.1 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = +2 Query: 254 SPGTSAATNPIAMATVFKNTVFTNVAETPE 343 S G ++ +P V KN+V +VAE PE Sbjct: 79 STGDASTQSPETSENVVKNSVDESVAEKPE 108 >SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 855 Score = 25.0 bits (52), Expect = 9.1 Identities = 17/68 (25%), Positives = 34/68 (50%), Gaps = 1/68 (1%) Frame = -3 Query: 612 SSHEHAVLPVPRLDYERYAGRPSAAQQDGGYRHTLEL-SHSPSIMGHCSAGVQNLEFGCA 436 +S +HA V LD++ PS+ ++GG+ ++ +H ++ + + E G + Sbjct: 255 ASPKHADTDVLGLDFDMTPSEPSSFPENGGFPSFVDANTHEQTLFPSSATNSFSFEHG-S 313 Query: 435 AGVFLLGS 412 AG + GS Sbjct: 314 AGFPIPGS 321 >SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|Schizosaccharomyces pombe|chr 1|||Manual Length = 512 Score = 25.0 bits (52), Expect = 9.1 Identities = 9/23 (39%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = +2 Query: 455 FCTP-AEQCPMIDGEWESSRVCR 520 FCT + +CP +D W+ R+C+ Sbjct: 386 FCTGISSKCP-VDENWDDGRICQ 407 >SPBC31F10.10c |||zf-MYND type zinc finger protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 574 Score = 25.0 bits (52), Expect = 9.1 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = +1 Query: 505 LEGVPISAILLGGRRPAGV 561 L GVP ++ LL GRRP V Sbjct: 352 LPGVPSTSALLDGRRPENV 370 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,038,040 Number of Sequences: 5004 Number of extensions: 71529 Number of successful extensions: 252 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 226 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 252 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 281707720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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