Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0318
(658 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 66 4e-12
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 48 1e-06
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 48 2e-06
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 45 1e-05
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 41 2e-04
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 35 0.012
SPCC1753.05 |rsm1||RNA export factor Rsm1|Schizosaccharomyces po... 32 0.084
SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7 |Schizos... 29 0.45
SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyc... 28 1.4
SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 27 3.1
SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 26 5.5
SPBC800.08 |gcd10||translation initiation factor eIF-3 gamma sub... 25 7.3
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 66.1 bits (154), Expect = 4e-12
Identities = 30/83 (36%), Positives = 49/83 (59%)
Frame = +3
Query: 3 QYVDSKTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKTWSKSTVLTRQQLMF 182
+++ S E+ NPAT E+IG+V + + +E A+ AA A+KT+ T + R QL+
Sbjct: 25 KWISSPNNKTFEVDNPATGEIIGKVADVSVEETKKAISAANEAFKTYKNFTHVQRSQLLE 84
Query: 183 KFARLLRENQSKLAAKITEEQGK 251
++A L+ EN+ L +T E GK
Sbjct: 85 RWAELIMENKDDLVKMLTLENGK 107
Score = 26.6 bits (56), Expect = 3.1
Identities = 15/45 (33%), Positives = 21/45 (46%)
Frame = +2
Query: 374 SYKVPLGVVGGVAAFNFPVMIPLWMFPPALVTGNTCIIKPSEQDP 508
S K P+GV + +NFP + AL G T I P+ + P
Sbjct: 150 SIKQPVGVSALITPWNFPAAMIARKGGAALAAGCTAIFLPAFRTP 194
Score = 26.2 bits (55), Expect = 4.2
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +1
Query: 517 LMMMELLQEAGAPPGVVNII 576
L ++ L QEAG P GV+N+I
Sbjct: 198 LGLVRLAQEAGFPDGVLNVI 217
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 48.0 bits (109), Expect = 1e-06
Identities = 18/43 (41%), Positives = 28/43 (65%)
Frame = +2
Query: 380 KVPLGVVGGVAAFNFPVMIPLWMFPPALVTGNTCIIKPSEQDP 508
+ P GV+G + +NFP+ + LW PA+ +GN ++KPSE P
Sbjct: 160 RFPRGVIGVITPWNFPLKMALWKLVPAIASGNCVVLKPSELAP 202
Score = 43.6 bits (98), Expect = 3e-05
Identities = 26/78 (33%), Positives = 42/78 (53%), Gaps = 2/78 (2%)
Frame = +3
Query: 33 IELTNPATNEVIGRVPEATQDELTSALDAAKRAYKT--WSKSTVLTRQQLMFKFARLLRE 206
I L NPAT E+IG A+ ++ SA++ A +++ W+K R ++ K A+++RE
Sbjct: 40 IPLINPATEEIIGTCANASAKDVDSAVENAYNTFRSGIWAKWPGKQRGLVLRKIAKMMRE 99
Query: 207 NQSKLAAKITEEQGKT*P 260
+ LA T GK P
Sbjct: 100 KRELLAGIDTINCGKPTP 117
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 47.6 bits (108), Expect = 2e-06
Identities = 26/78 (33%), Positives = 41/78 (52%)
Frame = +3
Query: 18 KTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKTWSKSTVLTRQQLMFKFARL 197
K+ E +P+ EVI +V A + E+ A+ +AK A+KTW K + +L+ K A L
Sbjct: 31 KSAETWETVDPSIEEVIAKVYLAGEKEIDYAVKSAKEAFKTWKKVPGSEKGELLMKLAEL 90
Query: 198 LRENQSKLAAKITEEQGK 251
++ LAA + GK
Sbjct: 91 TEKHADTLAAIEAMDSGK 108
Score = 47.6 bits (108), Expect = 2e-06
Identities = 25/85 (29%), Positives = 42/85 (49%)
Frame = +2
Query: 254 LADAEGDVLRGIQSVEHCCSITSLQLGDSIQNIAKDMDTHSYKVPLGVVGGVAAFNFPVM 433
+++A GDV I + +C G I + + ++ + P+GV G + +N+P+
Sbjct: 111 VSNARGDVDGTIALLRYCAGWADKIYGQVIPTGPEKL-AYAKRTPIGVCGQIVPWNYPLN 169
Query: 434 IPLWMFPPALVTGNTCIIKPSEQDP 508
+ W PAL GN IIK +E P
Sbjct: 170 MAGWKIAPALAAGNCIIIKSAETTP 194
Score = 28.3 bits (60), Expect = 1.0
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +1
Query: 517 LMMMELLQEAGAPPGVVNIIHG 582
L L++EAG P GVVNII G
Sbjct: 198 LYFATLVEEAGFPKGVVNIISG 219
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 44.8 bits (101), Expect = 1e-05
Identities = 24/82 (29%), Positives = 37/82 (45%)
Frame = +2
Query: 263 AEGDVLRGIQSVEHCCSITSLQLGDSIQNIAKDMDTHSYKVPLGVVGGVAAFNFPVMIPL 442
A GDV + G +I+ K ++ P+GV G + +NFP ++
Sbjct: 120 ARGDVQAAADCFRYYGGWADKDYGQTIETDIKRF-AYTRHEPIGVCGQIIPWNFPFLMCA 178
Query: 443 WMFPPALVTGNTCIIKPSEQDP 508
W PA+ GNT I+K +E P
Sbjct: 179 WKIAPAVACGNTIILKTAELTP 200
Score = 44.0 bits (99), Expect = 2e-05
Identities = 28/109 (25%), Positives = 57/109 (52%), Gaps = 9/109 (8%)
Frame = +3
Query: 3 QYVDSKTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKT---WSKSTVLTRQQ 173
Q+VDS +++ +P+T ++I V +A ++++ A+ A+ A++T W K + R +
Sbjct: 30 QHVDSVHGGRVKVYSPSTEKLICEVADADEEDVDIAVKVARAAFQTDAPWRKFSSAQRGR 89
Query: 174 LMFKFARLLRENQSKLAAKITEEQGKT*PMLR------AMCFEEFSQWS 302
+ + A + +N LA+ T + GK+ + R A CF + W+
Sbjct: 90 CLSRLADCIEQNLEYLASIETLDNGKSITLARGDVQAAADCFRYYGGWA 138
Score = 27.5 bits (58), Expect = 1.8
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +1
Query: 511 ATLMMMELLQEAGAPPGVVNIIHG 582
+ L + + + E G PPGV+N++ G
Sbjct: 202 SALCLTKFVPECGFPPGVINVLSG 225
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 40.7 bits (91), Expect = 2e-04
Identities = 24/85 (28%), Positives = 40/85 (47%)
Frame = +2
Query: 254 LADAEGDVLRGIQSVEHCCSITSLQLGDSIQNIAKDMDTHSYKVPLGVVGGVAAFNFPVM 433
L DA+G+V+ + ++ GDS + + K P+GVVG + +NFP
Sbjct: 115 LGDAKGEVVYAAKFIDWFAGEALRISGDSSMSSNPQNRIITIKQPVGVVGIITPWNFPAA 174
Query: 434 IPLWMFPPALVTGNTCIIKPSEQDP 508
+ AL G T +I+P+ + P
Sbjct: 175 MITRKVGAALAAGCTVVIRPAAETP 199
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 34.7 bits (76), Expect = 0.012
Identities = 22/68 (32%), Positives = 36/68 (52%)
Frame = +2
Query: 386 PLGVVGGVAAFNFPVMIPLWMFPPALVTGNTCIIKPSEQDPEPRS**WSSFRKPGLLRAL 565
PLGV+ + ++N+P+ L AL GN ++K SE WS+ + ++R+L
Sbjct: 127 PLGVIAALVSWNYPLHNALGPIISALFAGNAIVVKGSELTA------WSTHQYCEMVRSL 180
Query: 566 LISFTGHT 589
L S GH+
Sbjct: 181 LQSM-GHS 187
Score = 31.5 bits (68), Expect = 0.11
Identities = 15/69 (21%), Positives = 30/69 (43%)
Frame = +3
Query: 48 PATNEVIGRVPEATQDELTSALDAAKRAYKTWSKSTVLTRQQLMFKFARLLRENQSKLAA 227
P ++G V + ++ ++ A+ A K W ++ R+ + + NQ K A
Sbjct: 10 PGDGSLLGEVKLFNKSDIDQSIILAEEAQKEWKSTSFAERRNFLKALKENIIRNQDKYAE 69
Query: 228 KITEEQGKT 254
++ GKT
Sbjct: 70 IACKDTGKT 78
>SPCC1753.05 |rsm1||RNA export factor Rsm1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 296
Score = 31.9 bits (69), Expect = 0.084
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Frame = +3
Query: 42 TNPATNEVIG---RVPEATQDELTSALDAAKRAYKTWSKSTVLTR 167
T+ TNE++ ++ E +D L +L A+K YK WS+ L R
Sbjct: 5 TDMETNEILDQLDKIDERNEDILLKSLKASKCTYKPWSREEFLRR 49
>SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 721
Score = 29.5 bits (63), Expect = 0.45
Identities = 17/44 (38%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = +3
Query: 6 YVDSKTTNWIELTNPATNEVIGRV-PEATQDELTSALDAAKRAY 134
Y ++T W+EL NPA EV G + E + E T+++ A RA+
Sbjct: 498 YEPARTGEWMELLNPA--EVFGNIMVEYSYPECTTSVILALRAF 539
>SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 529
Score = 27.9 bits (59), Expect = 1.4
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +2
Query: 380 KVPLGVVGGVAAFNFPVMIPLWMFPPALVTGNTCI 484
K +GVVGG +P P+ P ++TGN +
Sbjct: 291 KGQVGVVGGYPVMGYPPPSPVLQKPDLIMTGNRTV 325
>SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/47 (29%), Positives = 28/47 (59%)
Frame = +2
Query: 230 NH*RARENLADAEGDVLRGIQSVEHCCSITSLQLGDSIQNIAKDMDT 370
NH + ENL + + R I+++E +T+L+LG + + +++DT
Sbjct: 123 NHLKGLENLFFVQNRIRR-IENLEGLDRLTNLELGGNKIRVIENLDT 168
>SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2812
Score = 25.8 bits (54), Expect = 5.5
Identities = 17/56 (30%), Positives = 23/56 (41%), Gaps = 2/56 (3%)
Frame = -1
Query: 514 WLRILLR-RLDYTRVSGYQRWREHPQWDHHREIEGCNTS-NNSKWHLVAMRVHVFG 353
W R+ LR +L Y W WD HR I N NS + + ++ FG
Sbjct: 285 WKRLDLRLQLIQCLAISYPLWSNSETWDPHRSIRSFNLDLLNSSFFSLKNFLNFFG 340
>SPBC800.08 |gcd10||translation initiation factor eIF-3 gamma
subunit Gcd10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 462
Score = 25.4 bits (53), Expect = 7.3
Identities = 12/41 (29%), Positives = 19/41 (46%)
Frame = +3
Query: 18 KTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKT 140
KT NW ++TNP V + + EL ++ Y+T
Sbjct: 252 KTLNWYQVTNPTETLSEYSVEDIPESELNEMKLRHRKRYET 292
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,603,973
Number of Sequences: 5004
Number of extensions: 50710
Number of successful extensions: 130
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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