Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0310
(673 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1142.07c |vps32|snf7|vacuolar sorting protein Vps32|Schizosa... 77 3e-15
SPBC215.14c |vps20||vacuolar sorting protein Vps20|Schizosacchar... 36 0.005
SPBP8B7.20c |||RNA methyltransferase Nop2 |Schizosaccharomyces p... 31 0.15
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 27 1.9
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 5.7
SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|... 25 7.5
SPBC29A10.03c |rlf2|SPBC365.19c|chromatin remodeling complex sub... 25 7.5
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 25 7.5
>SPAC1142.07c |vps32|snf7|vacuolar sorting protein
Vps32|Schizosaccharomyces pombe|chr 1|||Manual
Length = 222
Score = 76.6 bits (180), Expect = 3e-15
Identities = 38/83 (45%), Positives = 52/83 (62%)
Frame = +3
Query: 258 ALKRKKRYEKQLTQIDGTLTQIEAQREALEGANTNAQVLNTMREAANAMKLAHKDIDVDK 437
ALKRKK +E +L +I+G+ IE Q +++ AN N + L MR+ A AMK + +D DK
Sbjct: 63 ALKRKKMHENELVKIEGSRNNIEQQLFSIQNANLNFETLQAMRQGAEAMKSIQRGMDADK 122
Query: 438 VHDIMDDIAEQHDISREITEAIS 506
V IMD I +Q IS EI+ IS
Sbjct: 123 VDQIMDKIRDQQTISEEISTMIS 145
Score = 40.3 bits (90), Expect = 2e-04
Identities = 21/61 (34%), Positives = 35/61 (57%)
Frame = +1
Query: 118 TTHEAIQKLRETEELLIKKQEFLEKKIDLEVQTARKHGTKNKRAAMQLSSGRRDTRSNLL 297
TT + I + +E L KK+E LE++I + + ARK+ T NKR A+ ++ + L+
Sbjct: 16 TTKDTIVRFQEMLALYDKKEEVLERQIAEQTEIARKNATTNKRLALTALKRKKMHENELV 75
Query: 298 K 300
K
Sbjct: 76 K 76
>SPBC215.14c |vps20||vacuolar sorting protein
Vps20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 226
Score = 35.9 bits (79), Expect = 0.005
Identities = 22/89 (24%), Positives = 40/89 (44%)
Frame = +3
Query: 237 EQKSCHAALKRKKRYEKQLTQIDGTLTQIEAQREALEGANTNAQVLNTMREAANAMKLAH 416
+++ ALK KK Y +TQ G L IE +E V+ ++E N ++
Sbjct: 51 DKRGALRALKAKKLYSGLITQTYGQLGNIEQLLSTIEFTLIQKDVMFGLQEGTNLIRQLQ 110
Query: 417 KDIDVDKVHDIMDDIAEQHDISREITEAI 503
D+ +++V I +D E E+ + +
Sbjct: 111 ADMPLERVGRICNDRDEAMSYVDEVNDML 139
>SPBP8B7.20c |||RNA methyltransferase Nop2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 608
Score = 31.1 bits (67), Expect = 0.15
Identities = 21/92 (22%), Positives = 40/92 (43%)
Frame = +3
Query: 240 QKSCHAALKRKKRYEKQLTQIDGTLTQIEAQREALEGANTNAQVLNTMREAANAMKLAHK 419
+K H +LK +RY + IDG A+ + + V + M++ N
Sbjct: 513 EKRFHPSLKLTRRYYPHVHNIDGFFV---AKLKKISDKIPTVNVADDMKDGTN------N 563
Query: 420 DIDVDKVHDIMDDIAEQHDISREITEAISKMW 515
D++++K +D+I + +EI E + W
Sbjct: 564 DVEIEKNSTEIDNITFNDEADKEIIEQNRRKW 595
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 27.5 bits (58), Expect = 1.9
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +3
Query: 177 RVFGKEDRFRSTDSQETWH*EQKSC 251
+ F E RFRSTD WH + C
Sbjct: 927 KTFAAEIRFRSTDGHYRWHLVKSVC 951
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 25.8 bits (54), Expect = 5.7
Identities = 21/77 (27%), Positives = 32/77 (41%)
Frame = +3
Query: 279 YEKQLTQIDGTLTQIEAQREALEGANTNAQVLNTMREAANAMKLAHKDIDVDKVHDIMDD 458
YE+ ++ + AQR LE A L T REA A + A K K +
Sbjct: 546 YERAQAGVEQERKAVIAQRSLLELRRGQADTLATQREAELAAQRALK----QKQESEAES 601
Query: 459 IAEQHDISREITEAISK 509
+ Q +I++ E I +
Sbjct: 602 LRVQEEINKRNAERIRR 618
>SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1402
Score = 25.4 bits (53), Expect = 7.5
Identities = 11/32 (34%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = +1
Query: 145 RETEELLIKKQEFLEK-KIDLEVQTARKHGTK 237
++TEEL ++KQ F+E+ ++ + Q H K
Sbjct: 608 KDTEELTLQKQSFIERDRMQMISQMRNMHDNK 639
>SPBC29A10.03c |rlf2|SPBC365.19c|chromatin remodeling complex
subunit Rlf2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 544
Score = 25.4 bits (53), Expect = 7.5
Identities = 17/43 (39%), Positives = 23/43 (53%), Gaps = 4/43 (9%)
Frame = +1
Query: 127 EAIQKLRETEELLIKKQEFLEK----KIDLEVQTARKHGTKNK 243
E +KLRE E++ KK + LEK +I L+ Q RK K
Sbjct: 108 EQEKKLREQEKIAAKKMKELEKLEKERIRLQEQQRRKEERDQK 150
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 25.4 bits (53), Expect = 7.5
Identities = 13/37 (35%), Positives = 24/37 (64%)
Frame = +1
Query: 139 KLRETEELLIKKQEFLEKKIDLEVQTARKHGTKNKRA 249
KL E+ + LIKKQE ++ ++ +QT ++ K++ A
Sbjct: 669 KLEESNKSLIKKQEDVD-SLEKNIQTLKEDLRKSEEA 704
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,544,470
Number of Sequences: 5004
Number of extensions: 47223
Number of successful extensions: 191
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 307866294
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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