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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0308
         (711 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C...   128   6e-31
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ...    50   2e-07
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu...    46   5e-06
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit...    40   4e-04
SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C...    39   6e-04
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni...    34   0.023
SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro...    31   0.21 
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ...    29   0.50 
SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |S...    27   2.0  
SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein 4|Schizosacc...    27   2.0  
SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|...    27   3.5  
SPAC4G9.11c |cmb1||cytosine-mismatch binding protein 1|Schizosac...    26   4.6  
SPCC970.02 |||mannan endo-1,6-alpha-mannosidase|Schizosaccharomy...    26   4.6  
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po...    25   8.1  
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa...    25   8.1  
SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex ...    25   8.1  

>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
           Cct6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 535

 Score =  128 bits (310), Expect = 6e-31
 Identities = 54/85 (63%), Positives = 77/85 (90%)
 Frame = +3

Query: 255 EMKHKTATETVLVKGLVMDHGARHPDMPKRVENAYILTCNVSLEYEKTEVNSGFFYKSAE 434
           +M++++A++T L++GL++DHGARHPDMPK+V+NAYIL  NVSLEYEK+E+NSGFFY ++E
Sbjct: 193 KMQNRSASDTQLIRGLLLDHGARHPDMPKQVKNAYILILNVSLEYEKSEINSGFFYSTSE 252

Query: 435 DREKLVAAEREFIDQRVRKIVALKK 509
            RE+LV +ER+F+D ++RKIV LKK
Sbjct: 253 QRERLVESERKFVDNKLRKIVELKK 277



 Score =  100 bits (239), Expect = 2e-22
 Identities = 45/86 (52%), Positives = 65/86 (75%)
 Frame = +1

Query: 1   ISEGLHPRIITEGFDIARNKSLEVLESMKIPIEIARENLVDVARTSLKTKVHPSLADVLT 180
           I EGLHP +I++GF++A+N++L  L+S K   E+ RE L++VA+TSL TK+   + + L 
Sbjct: 108 IREGLHPSLISDGFNLAKNEALTFLDSFKTDFEVDREVLLNVAKTSLSTKISSKVVESLA 167

Query: 181 DACVDAVLTIRTPGKPVDLHMVEIMR 258
            A VDA+LTIR P +P+DLHMVEIM+
Sbjct: 168 PAVVDAILTIRRPDEPIDLHMVEIMK 193



 Score = 84.2 bits (199), Expect = 2e-17
 Identities = 46/71 (64%), Positives = 52/71 (73%), Gaps = 2/71 (2%)
 Frame = +2

Query: 503 QKKLC--DGTDNTFVVINQKGIDPLSLDAFAKEGIIGLRRAKRRNMERLALACGGGPLLT 676
           +K++C  D T N FV+INQKGIDPLSLD  AK GI+ LRRAKRRNMERL LAC GG    
Sbjct: 276 KKEVCERDPTAN-FVIINQKGIDPLSLDVLAKNGIMALRRAKRRNMERLQLAC-GGVAQN 333

Query: 677 LVDDLTEDALG 709
            VDDL  + LG
Sbjct: 334 SVDDLNPEVLG 344


>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
           Cct4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 527

 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 28/89 (31%), Positives = 51/89 (57%), Gaps = 3/89 (3%)
 Frame = +1

Query: 1   ISEGLHPRIITEGFDIARNKSLEVLESMKIPIEIA-RENLVDVARTSLKTKVHPSLADVL 177
           + +G+HP +I E F  A   +++ ++   + IE++ RE+L+  A TSL +K+    +++L
Sbjct: 111 LKKGIHPTVIAESFQRAAGFTVDCMKENALAIELSDRESLLRAATTSLNSKIVSQYSNLL 170

Query: 178 TDACVDAVLTIRTP--GKPVDLHMVEIMR 258
               VDAVL +  P     VDL  + I++
Sbjct: 171 APIAVDAVLKVIDPRVATNVDLKDIRIVK 199



 Score = 31.1 bits (67), Expect = 0.16
 Identities = 23/90 (25%), Positives = 40/90 (44%), Gaps = 1/90 (1%)
 Frame = +3

Query: 279 ETVLVKGLVMDHGA-RHPDMPKRVENAYILTCNVSLEYEKTEVNSGFFYKSAEDREKLVA 455
           +T L+ GL +   A +    P R+E A I      L   K ++ +          +K++ 
Sbjct: 207 DTELIPGLALTQTAVKSAGGPTRIEKANIALIQFQLSPPKPDMENQVVVNDYRQMDKILK 266

Query: 456 AEREFIDQRVRKIVALKKNYATVLTILSSL 545
            ER+++    +KI   KK  A V+ I  S+
Sbjct: 267 EERQYLLNMCKKI---KKAGANVILIQKSI 293


>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
           Cct5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 546

 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 24/72 (33%), Positives = 35/72 (48%)
 Frame = +3

Query: 279 ETVLVKGLVMDHGARHPDMPKRVENAYILTCNVSLEYEKTEVNSGFFYKSAEDREKLVAA 458
           +T LVKG+V+D    HP MP R+ENA I       E  K +        S  + E L A 
Sbjct: 223 DTKLVKGVVVDKDMSHPQMPHRIENAKIAILTCPFEPPKPKTKHKLDITSVSEFEALQAY 282

Query: 459 EREFIDQRVRKI 494
           E+E   + ++ +
Sbjct: 283 EKEKFQEMIKHV 294



 Score = 42.3 bits (95), Expect = 7e-05
 Identities = 25/88 (28%), Positives = 50/88 (56%), Gaps = 4/88 (4%)
 Frame = +1

Query: 1   ISEGLHPRIITEGFDIARNKSLEVLESMKIPIEIARENLVDV---ARTSLKTKVHPSLAD 171
           I +G+HP  I +G++ A   +++ L+++   ++ + EN  ++   A+TSL +KV     D
Sbjct: 126 IDKGIHPIRIADGYEKACQVAVKHLDAISDVVDFSPENTTNLFRSAKTSLGSKVVSKAHD 185

Query: 172 VLTDACVDAVLTIR-TPGKPVDLHMVEI 252
              +  VDAVL++     K VD  ++++
Sbjct: 186 HFANIAVDAVLSVADLQRKDVDFELIKV 213


>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
           Cct3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 528

 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 19/73 (26%), Positives = 37/73 (50%)
 Frame = +3

Query: 288 LVKGLVMDHGARHPDMPKRVENAYILTCNVSLEYEKTEVNSGFFYKSAEDREKLVAAERE 467
           ++ G++++    HP M +R+EN  I+  +  LEY K E  +        D  +++  E E
Sbjct: 212 VLDGVMLNKDVTHPKMRRRIENPRIVLLDCPLEYRKGESQTNIEISKDTDWNRILEIEEE 271

Query: 468 FIDQRVRKIVALK 506
            + +    I+A+K
Sbjct: 272 QVKRMCDYIIAVK 284



 Score = 38.7 bits (86), Expect = 8e-04
 Identities = 22/70 (31%), Positives = 37/70 (52%), Gaps = 2/70 (2%)
 Frame = +2

Query: 506 KKLCDG--TDNTFVVINQKGIDPLSLDAFAKEGIIGLRRAKRRNMERLALACGGGPLLTL 679
           K++CD        +VI +KG+  L+     K  I  LRR ++ +  R+A ACG   ++  
Sbjct: 274 KRMCDYIIAVKPDLVITEKGVSDLAQHYLLKANITALRRTRKSDNNRIARACGAN-IVNR 332

Query: 680 VDDLTEDALG 709
           ++DL E  +G
Sbjct: 333 LEDLREKDVG 342



 Score = 30.3 bits (65), Expect = 0.28
 Identities = 14/67 (20%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
 Frame = +1

Query: 13  LHPRIITEGFDIARNKSLEVLESMKIPIEI-ARENLVDVARTSLKTKVHPSLADVLTDAC 189
           +HP ++   F  A   +L +++ + +P+ +     +  + RT + TK+    +D++    
Sbjct: 115 IHPVVMIRSFKQALEDALSIIDEITLPVNVDDNAEMFRLIRTCIGTKLVARWSDLMCHLA 174

Query: 190 VDAVLTI 210
           + AV T+
Sbjct: 175 LRAVRTV 181


>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
           Cct2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 527

 Score = 39.1 bits (87), Expect = 6e-04
 Identities = 22/90 (24%), Positives = 49/90 (54%), Gaps = 5/90 (5%)
 Frame = +1

Query: 1   ISEGLHPRIITEGFDIARNKSLEVLESMKI-----PIEIARENLVDVARTSLKTKVHPSL 165
           ++  +HP++I +G+ IA   +++ L +  I     P +  R +L ++ART+L +K+    
Sbjct: 113 VNAKIHPQVIIDGYRIATKTAIDALRASSIDNSSDPAKF-RSDLENIARTTLSSKILSQN 171

Query: 166 ADVLTDACVDAVLTIRTPGKPVDLHMVEIM 255
            +      VDAVL ++      ++ +++I+
Sbjct: 172 KNHFAQLAVDAVLRLKGSTNLDNIQIIKIL 201


>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
           Cct1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 33.9 bits (74), Expect = 0.023
 Identities = 22/77 (28%), Positives = 37/77 (48%)
 Frame = +3

Query: 267 KTATETVLVKGLVMDHGARHPDMPKRVENAYILTCNVSLEYEKTEVNSGFFYKSAEDREK 446
           K++ E+VLVKG  ++       M  RV+NA I   ++ L+  K  +         +  EK
Sbjct: 208 KSSRESVLVKGYALNCTIASQAMKTRVQNAKIAVLDMDLQKTKMALGVHVTIDDPDQLEK 267

Query: 447 LVAAEREFIDQRVRKIV 497
           +   E     +RV+KI+
Sbjct: 268 IREREVMITLERVKKIL 284


>SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting protein
            3 homolog Bud6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1385

 Score = 30.7 bits (66), Expect = 0.21
 Identities = 22/72 (30%), Positives = 37/72 (51%), Gaps = 1/72 (1%)
 Frame = +3

Query: 348  ENAYILTCNVSLEYEKTEVNSGFFYKSAEDRE-KLVAAEREFIDQRVRKIVALKKNYATV 524
            E+AY+     +LE    EV     +K   +RE   +  E+EF+D     I  LK++ + +
Sbjct: 1060 ESAYLEKRIQTLERSLEEVTP--IWKKQWERELNAIVQEQEFLDSHTVLISDLKRDLSAL 1117

Query: 525  LTILSSLSTKKE 560
             T+LS++S   E
Sbjct: 1118 STVLSNVSAIAE 1129


>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
           Cct7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 558

 Score = 29.5 bits (63), Expect = 0.50
 Identities = 18/75 (24%), Positives = 39/75 (52%), Gaps = 5/75 (6%)
 Frame = +1

Query: 1   ISEGLHPRIITEGFDIARNKSLEVLESMKIPIEIA-----RENLVDVARTSLKTKVHPSL 165
           + +G+   +I  G+  A   ++  ++ + I ++++     R+ L   A T++ +K+  S 
Sbjct: 116 VEDGVSSHLIIRGYRKAAQLAVNKIKEIAIHLDLSDEGKLRDLLTKCASTAMNSKLIRSN 175

Query: 166 ADVLTDACVDAVLTI 210
           +   T   VDAVLT+
Sbjct: 176 STFFTKMVVDAVLTL 190


>SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 690

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 12/23 (52%), Positives = 13/23 (56%)
 Frame = -3

Query: 199 PHQRKHQLAHLPSLGGP*F*VKY 131
           PHQR H  A L   GGP F + Y
Sbjct: 63  PHQRAHNAAALARSGGPGFSMNY 85


>SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein
           4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 636

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 12/42 (28%), Positives = 22/42 (52%)
 Frame = +2

Query: 485 KKDRCSQKKLCDGTDNTFVVINQKGIDPLSLDAFAKEGIIGL 610
           K D C   +LC G D   +++    ++P+  D F K+ + G+
Sbjct: 428 KGDYCEVMELCSGGDLYTLIMAAGRLEPMEADCFFKQLMRGV 469


>SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 997

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 10/24 (41%), Positives = 11/24 (45%)
 Frame = +2

Query: 284  CTCKRSSNGSWCTSPRYAKACRKC 355
            C  +R SNG WCT     K    C
Sbjct: 973  CIIERDSNGRWCTKHLQRKTKEVC 996


>SPAC4G9.11c |cmb1||cytosine-mismatch binding protein
           1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 223

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 9/15 (60%), Positives = 13/15 (86%)
 Frame = +3

Query: 321 RHPDMPKRVENAYIL 365
           RHPD+PK+  +A+IL
Sbjct: 142 RHPDVPKKPSSAFIL 156


>SPCC970.02 |||mannan endo-1,6-alpha-mannosidase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 442

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = +3

Query: 369 CNVSLEYEKTEVNSGFFYKS 428
           CN  L ++ TE NSG+ YK+
Sbjct: 162 CNGGLRWQITEFNSGYNYKN 181


>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 665

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 11/38 (28%), Positives = 19/38 (50%)
 Frame = +3

Query: 327 PDMPKRVENAYILTCNVSLEYEKTEVNSGFFYKSAEDR 440
           PD    V+NA + T +++     T V+ GF +  +  R
Sbjct: 11  PDFASDVDNASLFTQSIATTSASTSVDDGFSFDDSISR 48


>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1072

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 11/43 (25%), Positives = 27/43 (62%)
 Frame = +1

Query: 55  NKSLEVLESMKIPIEIARENLVDVARTSLKTKVHPSLADVLTD 183
           ++SL++L+     + ++ ++ VD+ART     ++ S ++V+ D
Sbjct: 270 SESLDLLDDFNYQVTVSNKSFVDIARTEYFITIY-SDSNVILD 311


>SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex
           subunit |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 509

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 12/21 (57%), Positives = 15/21 (71%)
 Frame = +1

Query: 28  ITEGFDIARNKSLEVLESMKI 90
           I EGF IAR K +E LE +K+
Sbjct: 448 IAEGFLIARAKRVESLERVKV 468


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,830,247
Number of Sequences: 5004
Number of extensions: 56661
Number of successful extensions: 176
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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